Amino acid dipepetide frequency for Gordonia phage Getalong

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.522AlaAla: 13.522 ± 1.144
0.842AlaCys: 0.842 ± 0.274
7.35AlaAsp: 7.35 ± 0.749
8.528AlaGlu: 8.528 ± 0.886
2.861AlaPhe: 2.861 ± 0.73
8.753AlaGly: 8.753 ± 0.876
1.908AlaHis: 1.908 ± 0.368
5.723AlaIle: 5.723 ± 0.693
3.535AlaLys: 3.535 ± 0.491
9.763AlaLeu: 9.763 ± 0.714
3.366AlaMet: 3.366 ± 0.544
3.142AlaAsn: 3.142 ± 0.468
5.218AlaPro: 5.218 ± 0.574
4.881AlaGln: 4.881 ± 0.653
6.677AlaArg: 6.677 ± 0.637
6.396AlaSer: 6.396 ± 0.59
5.667AlaThr: 5.667 ± 0.667
8.248AlaVal: 8.248 ± 1.06
2.188AlaTrp: 2.188 ± 0.378
2.357AlaTyr: 2.357 ± 0.328
0.0AlaXaa: 0.0 ± 0.0
Cys
1.122CysAla: 1.122 ± 0.308
0.337CysCys: 0.337 ± 0.247
0.954CysAsp: 0.954 ± 0.244
0.673CysGlu: 0.673 ± 0.225
0.337CysPhe: 0.337 ± 0.153
1.739CysGly: 1.739 ± 0.507
0.729CysHis: 0.729 ± 0.243
0.449CysIle: 0.449 ± 0.152
0.393CysLys: 0.393 ± 0.171
0.898CysLeu: 0.898 ± 0.237
0.112CysMet: 0.112 ± 0.08
0.505CysAsn: 0.505 ± 0.245
0.617CysPro: 0.617 ± 0.198
0.561CysGln: 0.561 ± 0.185
1.066CysArg: 1.066 ± 0.366
1.29CysSer: 1.29 ± 0.454
0.561CysThr: 0.561 ± 0.195
1.01CysVal: 1.01 ± 0.323
0.393CysTrp: 0.393 ± 0.137
0.281CysTyr: 0.281 ± 0.117
0.0CysXaa: 0.0 ± 0.0
Asp
7.126AspAla: 7.126 ± 0.638
0.673AspCys: 0.673 ± 0.184
4.264AspAsp: 4.264 ± 0.587
4.545AspGlu: 4.545 ± 0.545
1.908AspPhe: 1.908 ± 0.358
5.162AspGly: 5.162 ± 0.594
1.459AspHis: 1.459 ± 0.288
3.086AspIle: 3.086 ± 0.471
1.908AspLys: 1.908 ± 0.329
5.274AspLeu: 5.274 ± 0.702
1.178AspMet: 1.178 ± 0.242
1.403AspAsn: 1.403 ± 0.308
4.489AspPro: 4.489 ± 0.395
1.739AspGln: 1.739 ± 0.384
4.376AspArg: 4.376 ± 0.658
3.928AspSer: 3.928 ± 0.458
3.086AspThr: 3.086 ± 0.404
6.172AspVal: 6.172 ± 0.51
1.178AspTrp: 1.178 ± 0.247
1.795AspTyr: 1.795 ± 0.35
0.0AspXaa: 0.0 ± 0.0
Glu
7.406GluAla: 7.406 ± 0.635
0.898GluCys: 0.898 ± 0.268
3.198GluAsp: 3.198 ± 0.428
2.974GluGlu: 2.974 ± 0.504
1.627GluPhe: 1.627 ± 0.247
3.928GluGly: 3.928 ± 0.438
1.683GluHis: 1.683 ± 0.355
2.357GluIle: 2.357 ± 0.324
2.525GluLys: 2.525 ± 0.487
6.116GluLeu: 6.116 ± 0.876
1.234GluMet: 1.234 ± 0.265
1.01GluAsn: 1.01 ± 0.193
2.805GluPro: 2.805 ± 0.492
2.693GluGln: 2.693 ± 0.496
4.601GluArg: 4.601 ± 0.629
3.366GluSer: 3.366 ± 0.529
3.198GluThr: 3.198 ± 0.36
3.647GluVal: 3.647 ± 0.471
1.627GluTrp: 1.627 ± 0.349
0.954GluTyr: 0.954 ± 0.22
0.0GluXaa: 0.0 ± 0.0
Phe
3.086PheAla: 3.086 ± 0.417
0.281PheCys: 0.281 ± 0.147
1.515PheAsp: 1.515 ± 0.341
1.515PheGlu: 1.515 ± 0.233
0.954PhePhe: 0.954 ± 0.2
2.749PheGly: 2.749 ± 0.426
0.449PheHis: 0.449 ± 0.143
0.954PheIle: 0.954 ± 0.237
1.122PheLys: 1.122 ± 0.23
1.459PheLeu: 1.459 ± 0.311
1.066PheMet: 1.066 ± 0.277
0.842PheAsn: 0.842 ± 0.275
1.571PhePro: 1.571 ± 0.356
1.01PheGln: 1.01 ± 0.207
1.964PheArg: 1.964 ± 0.346
1.683PheSer: 1.683 ± 0.349
1.964PheThr: 1.964 ± 0.268
1.739PheVal: 1.739 ± 0.345
0.449PheTrp: 0.449 ± 0.154
0.842PheTyr: 0.842 ± 0.242
0.0PheXaa: 0.0 ± 0.0
Gly
7.406GlyAla: 7.406 ± 1.127
0.842GlyCys: 0.842 ± 0.318
4.881GlyAsp: 4.881 ± 0.577
3.31GlyGlu: 3.31 ± 0.436
3.31GlyPhe: 3.31 ± 0.612
8.023GlyGly: 8.023 ± 1.202
1.795GlyHis: 1.795 ± 0.349
3.647GlyIle: 3.647 ± 0.665
3.366GlyLys: 3.366 ± 0.44
6.116GlyLeu: 6.116 ± 0.653
2.3GlyMet: 2.3 ± 0.349
2.244GlyAsn: 2.244 ± 0.333
3.254GlyPro: 3.254 ± 0.424
3.142GlyGln: 3.142 ± 0.413
6.396GlyArg: 6.396 ± 0.587
5.218GlySer: 5.218 ± 0.682
5.33GlyThr: 5.33 ± 0.551
7.238GlyVal: 7.238 ± 0.734
2.693GlyTrp: 2.693 ± 0.339
1.908GlyTyr: 1.908 ± 0.264
0.0GlyXaa: 0.0 ± 0.0
His
1.739HisAla: 1.739 ± 0.329
0.393HisCys: 0.393 ± 0.161
1.178HisAsp: 1.178 ± 0.284
1.122HisGlu: 1.122 ± 0.275
0.505HisPhe: 0.505 ± 0.164
1.795HisGly: 1.795 ± 0.275
0.505HisHis: 0.505 ± 0.19
1.403HisIle: 1.403 ± 0.298
0.561HisLys: 0.561 ± 0.234
1.964HisLeu: 1.964 ± 0.367
0.786HisMet: 0.786 ± 0.218
0.505HisAsn: 0.505 ± 0.168
1.178HisPro: 1.178 ± 0.235
0.954HisGln: 0.954 ± 0.212
1.908HisArg: 1.908 ± 0.364
0.842HisSer: 0.842 ± 0.231
1.571HisThr: 1.571 ± 0.309
1.964HisVal: 1.964 ± 0.327
0.729HisTrp: 0.729 ± 0.206
0.898HisTyr: 0.898 ± 0.267
0.0HisXaa: 0.0 ± 0.0
Ile
5.723IleAla: 5.723 ± 0.639
0.617IleCys: 0.617 ± 0.203
3.535IleAsp: 3.535 ± 0.45
2.581IleGlu: 2.581 ± 0.386
0.842IlePhe: 0.842 ± 0.274
4.825IleGly: 4.825 ± 0.485
0.729IleHis: 0.729 ± 0.265
1.403IleIle: 1.403 ± 0.319
2.132IleLys: 2.132 ± 0.484
3.086IleLeu: 3.086 ± 0.411
0.617IleMet: 0.617 ± 0.208
1.515IleAsn: 1.515 ± 0.312
2.918IlePro: 2.918 ± 0.321
1.683IleGln: 1.683 ± 0.274
3.591IleArg: 3.591 ± 0.384
1.908IleSer: 1.908 ± 0.328
3.142IleThr: 3.142 ± 0.485
3.142IleVal: 3.142 ± 0.453
0.729IleTrp: 0.729 ± 0.203
1.178IleTyr: 1.178 ± 0.328
0.0IleXaa: 0.0 ± 0.0
Lys
4.713LysAla: 4.713 ± 0.729
0.505LysCys: 0.505 ± 0.202
2.749LysAsp: 2.749 ± 0.498
1.403LysGlu: 1.403 ± 0.296
1.347LysPhe: 1.347 ± 0.292
2.525LysGly: 2.525 ± 0.532
0.898LysHis: 0.898 ± 0.241
1.122LysIle: 1.122 ± 0.186
2.3LysLys: 2.3 ± 0.528
2.749LysLeu: 2.749 ± 0.299
0.673LysMet: 0.673 ± 0.189
1.066LysAsn: 1.066 ± 0.296
1.627LysPro: 1.627 ± 0.3
1.122LysGln: 1.122 ± 0.27
3.142LysArg: 3.142 ± 0.455
1.627LysSer: 1.627 ± 0.384
2.974LysThr: 2.974 ± 0.384
2.469LysVal: 2.469 ± 0.43
0.842LysTrp: 0.842 ± 0.22
0.729LysTyr: 0.729 ± 0.183
0.0LysXaa: 0.0 ± 0.0
Leu
9.37LeuAla: 9.37 ± 0.919
0.842LeuCys: 0.842 ± 0.244
4.994LeuAsp: 4.994 ± 0.554
3.928LeuGlu: 3.928 ± 0.698
1.908LeuPhe: 1.908 ± 0.427
6.228LeuGly: 6.228 ± 0.642
1.627LeuHis: 1.627 ± 0.378
3.423LeuIle: 3.423 ± 0.423
2.525LeuLys: 2.525 ± 0.369
5.891LeuLeu: 5.891 ± 0.787
1.627LeuMet: 1.627 ± 0.281
1.739LeuAsn: 1.739 ± 0.303
4.489LeuPro: 4.489 ± 0.484
2.413LeuGln: 2.413 ± 0.361
5.947LeuArg: 5.947 ± 0.629
4.376LeuSer: 4.376 ± 0.492
5.611LeuThr: 5.611 ± 0.605
6.284LeuVal: 6.284 ± 0.725
1.571LeuTrp: 1.571 ± 0.38
1.683LeuTyr: 1.683 ± 0.344
0.0LeuXaa: 0.0 ± 0.0
Met
2.637MetAla: 2.637 ± 0.444
0.561MetCys: 0.561 ± 0.212
1.29MetAsp: 1.29 ± 0.279
0.842MetGlu: 0.842 ± 0.191
0.449MetPhe: 0.449 ± 0.153
1.683MetGly: 1.683 ± 0.292
0.729MetHis: 0.729 ± 0.26
0.954MetIle: 0.954 ± 0.217
0.729MetLys: 0.729 ± 0.222
1.178MetLeu: 1.178 ± 0.262
0.337MetMet: 0.337 ± 0.136
0.561MetAsn: 0.561 ± 0.179
1.683MetPro: 1.683 ± 0.306
0.673MetGln: 0.673 ± 0.172
2.02MetArg: 2.02 ± 0.462
2.469MetSer: 2.469 ± 0.31
2.413MetThr: 2.413 ± 0.428
1.908MetVal: 1.908 ± 0.361
0.449MetTrp: 0.449 ± 0.153
0.449MetTyr: 0.449 ± 0.16
0.0MetXaa: 0.0 ± 0.0
Asn
3.815AsnAla: 3.815 ± 0.476
0.393AsnCys: 0.393 ± 0.152
1.852AsnAsp: 1.852 ± 0.301
1.29AsnGlu: 1.29 ± 0.224
0.393AsnPhe: 0.393 ± 0.127
2.918AsnGly: 2.918 ± 0.437
0.729AsnHis: 0.729 ± 0.195
1.234AsnIle: 1.234 ± 0.277
0.449AsnLys: 0.449 ± 0.137
2.02AsnLeu: 2.02 ± 0.324
0.505AsnMet: 0.505 ± 0.177
0.729AsnAsn: 0.729 ± 0.235
2.132AsnPro: 2.132 ± 0.307
1.234AsnGln: 1.234 ± 0.305
2.525AsnArg: 2.525 ± 0.441
1.403AsnSer: 1.403 ± 0.266
1.795AsnThr: 1.795 ± 0.347
2.02AsnVal: 2.02 ± 0.393
0.393AsnTrp: 0.393 ± 0.141
0.617AsnTyr: 0.617 ± 0.177
0.0AsnXaa: 0.0 ± 0.0
Pro
6.172ProAla: 6.172 ± 0.625
1.234ProCys: 1.234 ± 0.313
4.432ProAsp: 4.432 ± 0.518
4.04ProGlu: 4.04 ± 0.559
1.234ProPhe: 1.234 ± 0.276
3.815ProGly: 3.815 ± 0.453
0.842ProHis: 0.842 ± 0.191
2.357ProIle: 2.357 ± 0.407
2.749ProLys: 2.749 ± 0.414
3.254ProLeu: 3.254 ± 0.478
1.234ProMet: 1.234 ± 0.281
1.515ProAsn: 1.515 ± 0.347
2.637ProPro: 2.637 ± 0.509
1.571ProGln: 1.571 ± 0.377
3.815ProArg: 3.815 ± 0.517
3.423ProSer: 3.423 ± 0.497
3.815ProThr: 3.815 ± 0.478
3.984ProVal: 3.984 ± 0.47
0.617ProTrp: 0.617 ± 0.182
1.515ProTyr: 1.515 ± 0.364
0.0ProXaa: 0.0 ± 0.0
Gln
3.815GlnAla: 3.815 ± 0.545
0.729GlnCys: 0.729 ± 0.197
1.515GlnAsp: 1.515 ± 0.304
1.964GlnGlu: 1.964 ± 0.268
0.954GlnPhe: 0.954 ± 0.208
2.525GlnGly: 2.525 ± 0.327
1.122GlnHis: 1.122 ± 0.307
1.795GlnIle: 1.795 ± 0.294
1.178GlnLys: 1.178 ± 0.277
2.861GlnLeu: 2.861 ± 0.389
1.066GlnMet: 1.066 ± 0.216
0.954GlnAsn: 0.954 ± 0.22
1.908GlnPro: 1.908 ± 0.237
1.964GlnGln: 1.964 ± 0.336
3.254GlnArg: 3.254 ± 0.441
2.076GlnSer: 2.076 ± 0.305
2.132GlnThr: 2.132 ± 0.421
2.749GlnVal: 2.749 ± 0.403
1.178GlnTrp: 1.178 ± 0.261
0.786GlnTyr: 0.786 ± 0.208
0.0GlnXaa: 0.0 ± 0.0
Arg
6.901ArgAla: 6.901 ± 0.586
1.066ArgCys: 1.066 ± 0.246
4.432ArgAsp: 4.432 ± 0.442
4.545ArgGlu: 4.545 ± 0.563
1.795ArgPhe: 1.795 ± 0.291
5.162ArgGly: 5.162 ± 0.583
2.244ArgHis: 2.244 ± 0.425
4.152ArgIle: 4.152 ± 0.533
3.31ArgLys: 3.31 ± 0.539
6.228ArgLeu: 6.228 ± 0.472
1.852ArgMet: 1.852 ± 0.32
2.805ArgAsn: 2.805 ± 0.375
4.376ArgPro: 4.376 ± 0.652
2.637ArgGln: 2.637 ± 0.355
6.228ArgArg: 6.228 ± 0.943
3.928ArgSer: 3.928 ± 0.556
3.984ArgThr: 3.984 ± 0.503
4.713ArgVal: 4.713 ± 0.557
1.739ArgTrp: 1.739 ± 0.304
2.188ArgTyr: 2.188 ± 0.32
0.0ArgXaa: 0.0 ± 0.0
Ser
6.06SerAla: 6.06 ± 0.572
0.617SerCys: 0.617 ± 0.255
3.984SerAsp: 3.984 ± 0.692
3.815SerGlu: 3.815 ± 0.49
1.571SerPhe: 1.571 ± 0.279
6.508SerGly: 6.508 ± 0.829
1.403SerHis: 1.403 ± 0.281
2.469SerIle: 2.469 ± 0.351
1.515SerLys: 1.515 ± 0.276
3.928SerLeu: 3.928 ± 0.48
2.357SerMet: 2.357 ± 0.342
1.683SerAsn: 1.683 ± 0.418
2.413SerPro: 2.413 ± 0.302
1.29SerGln: 1.29 ± 0.291
4.264SerArg: 4.264 ± 0.608
3.759SerSer: 3.759 ± 0.55
3.759SerThr: 3.759 ± 0.518
5.106SerVal: 5.106 ± 0.821
1.29SerTrp: 1.29 ± 0.221
0.954SerTyr: 0.954 ± 0.231
0.0SerXaa: 0.0 ± 0.0
Thr
7.182ThrAla: 7.182 ± 0.876
1.066ThrCys: 1.066 ± 0.571
3.535ThrAsp: 3.535 ± 0.401
3.254ThrGlu: 3.254 ± 0.605
1.515ThrPhe: 1.515 ± 0.257
4.601ThrGly: 4.601 ± 0.631
1.627ThrHis: 1.627 ± 0.324
3.759ThrIle: 3.759 ± 0.447
1.403ThrLys: 1.403 ± 0.3
5.779ThrLeu: 5.779 ± 0.576
0.729ThrMet: 0.729 ± 0.173
1.852ThrAsn: 1.852 ± 0.292
4.264ThrPro: 4.264 ± 0.5
2.525ThrGln: 2.525 ± 0.363
3.759ThrArg: 3.759 ± 0.425
3.928ThrSer: 3.928 ± 0.647
3.647ThrThr: 3.647 ± 0.498
5.386ThrVal: 5.386 ± 0.542
1.403ThrTrp: 1.403 ± 0.269
1.403ThrTyr: 1.403 ± 0.288
0.0ThrXaa: 0.0 ± 0.0
Val
9.089ValAla: 9.089 ± 0.831
1.403ValCys: 1.403 ± 0.464
5.218ValAsp: 5.218 ± 0.593
4.994ValGlu: 4.994 ± 0.486
2.413ValPhe: 2.413 ± 0.378
5.779ValGly: 5.779 ± 0.698
1.234ValHis: 1.234 ± 0.247
3.815ValIle: 3.815 ± 0.486
2.805ValLys: 2.805 ± 0.511
4.601ValLeu: 4.601 ± 0.472
1.795ValMet: 1.795 ± 0.314
2.525ValAsn: 2.525 ± 0.456
4.152ValPro: 4.152 ± 0.573
2.637ValGln: 2.637 ± 0.435
4.881ValArg: 4.881 ± 0.607
4.881ValSer: 4.881 ± 0.724
4.32ValThr: 4.32 ± 0.688
6.06ValVal: 6.06 ± 0.555
2.3ValTrp: 2.3 ± 0.385
1.627ValTyr: 1.627 ± 0.318
0.0ValXaa: 0.0 ± 0.0
Trp
2.02TrpAla: 2.02 ± 0.421
0.337TrpCys: 0.337 ± 0.126
1.571TrpAsp: 1.571 ± 0.275
1.347TrpGlu: 1.347 ± 0.315
0.561TrpPhe: 0.561 ± 0.149
1.29TrpGly: 1.29 ± 0.277
0.393TrpHis: 0.393 ± 0.17
1.178TrpIle: 1.178 ± 0.245
0.898TrpLys: 0.898 ± 0.225
1.964TrpLeu: 1.964 ± 0.376
0.617TrpMet: 0.617 ± 0.19
1.234TrpAsn: 1.234 ± 0.343
1.515TrpPro: 1.515 ± 0.3
1.178TrpGln: 1.178 ± 0.264
1.964TrpArg: 1.964 ± 0.311
0.842TrpSer: 0.842 ± 0.216
1.515TrpThr: 1.515 ± 0.275
1.178TrpVal: 1.178 ± 0.291
0.505TrpTrp: 0.505 ± 0.228
0.505TrpTyr: 0.505 ± 0.156
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.02TyrAla: 2.02 ± 0.374
0.393TyrCys: 0.393 ± 0.18
2.3TyrAsp: 2.3 ± 0.355
1.178TyrGlu: 1.178 ± 0.293
0.786TyrPhe: 0.786 ± 0.213
2.413TyrGly: 2.413 ± 0.377
0.393TyrHis: 0.393 ± 0.156
0.617TyrIle: 0.617 ± 0.218
1.178TyrLys: 1.178 ± 0.252
1.347TyrLeu: 1.347 ± 0.357
0.505TyrMet: 0.505 ± 0.144
0.617TyrAsn: 0.617 ± 0.182
1.01TyrPro: 1.01 ± 0.239
0.617TyrGln: 0.617 ± 0.234
1.908TyrArg: 1.908 ± 0.34
1.403TyrSer: 1.403 ± 0.282
2.132TyrThr: 2.132 ± 0.443
1.515TyrVal: 1.515 ± 0.244
0.337TyrTrp: 0.337 ± 0.144
0.673TyrTyr: 0.673 ± 0.183
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 104 proteins (17824 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski