Amino acid dipepetide frequency for Clostridium septicum

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	3.271AlaAla: 3.271 ± 0.085	0.609AlaCys: 0.609 ± 0.028	2.37AlaAsp: 2.37 ± 0.057	3.466AlaGlu: 3.466 ± 0.068	2.35AlaPhe: 2.35 ± 0.052	3.495AlaGly: 3.495 ± 0.076	0.746AlaHis: 0.746 ± 0.032	5.802AlaIle: 5.802 ± 0.093	4.669AlaLys: 4.669 ± 0.075	5.656AlaLeu: 5.656 ± 0.085	1.516AlaMet: 1.516 ± 0.05	2.842AlaAsn: 2.842 ± 0.066	1.393AlaPro: 1.393 ± 0.038	1.196AlaGln: 1.196 ± 0.039	1.705AlaArg: 1.705 ± 0.044	3.202AlaSer: 3.202 ± 0.065	2.941AlaThr: 2.941 ± 0.059	3.568AlaVal: 3.568 ± 0.071	0.339AlaTrp: 0.339 ± 0.026	1.994AlaTyr: 1.994 ± 0.051	0.0AlaXaa: 0.0 ± 0.0
Cys	0.479CysAla: 0.479 ± 0.024	0.186CysCys: 0.186 ± 0.02	0.608CysAsp: 0.608 ± 0.031	0.692CysGlu: 0.692 ± 0.028	0.436CysPhe: 0.436 ± 0.023	0.942CysGly: 0.942 ± 0.035	0.188CysHis: 0.188 ± 0.014	1.072CysIle: 1.072 ± 0.04	1.02CysLys: 1.02 ± 0.038	0.779CysLeu: 0.779 ± 0.031	0.229CysMet: 0.229 ± 0.017	0.775CysAsn: 0.775 ± 0.032	0.442CysPro: 0.442 ± 0.023	0.207CysGln: 0.207 ± 0.015	0.334CysArg: 0.334 ± 0.018	0.689CysSer: 0.689 ± 0.029	0.6CysThr: 0.6 ± 0.028	0.569CysVal: 0.569 ± 0.023	0.064CysTrp: 0.064 ± 0.008	0.423CysTyr: 0.423 ± 0.025	0.0CysXaa: 0.0 ± 0.0
Asp	2.468AspAla: 2.468 ± 0.064	0.512AspCys: 0.512 ± 0.025	2.613AspAsp: 2.613 ± 0.058	4.677AspGlu: 4.677 ± 0.079	2.762AspPhe: 2.762 ± 0.054	3.498AspGly: 3.498 ± 0.069	0.508AspHis: 0.508 ± 0.025	6.47AspIle: 6.47 ± 0.088	5.56AspLys: 5.56 ± 0.093	5.164AspLeu: 5.164 ± 0.077	1.426AspMet: 1.426 ± 0.038	3.543AspAsn: 3.543 ± 0.062	1.28AspPro: 1.28 ± 0.04	0.691AspGln: 0.691 ± 0.031	1.83AspArg: 1.83 ± 0.046	3.319AspSer: 3.319 ± 0.061	2.472AspThr: 2.472 ± 0.047	3.535AspVal: 3.535 ± 0.069	0.378AspTrp: 0.378 ± 0.025	2.569AspTyr: 2.569 ± 0.057	0.0AspXaa: 0.0 ± 0.0
Glu	4.271GluAla: 4.271 ± 0.078	0.665GluCys: 0.665 ± 0.027	4.383GluAsp: 4.383 ± 0.081	7.509GluGlu: 7.509 ± 0.129	3.294GluPhe: 3.294 ± 0.065	4.406GluGly: 4.406 ± 0.075	0.833GluHis: 0.833 ± 0.034	7.66GluIle: 7.66 ± 0.115	7.868GluLys: 7.868 ± 0.113	6.941GluLeu: 6.941 ± 0.095	1.877GluMet: 1.877 ± 0.046	5.904GluAsn: 5.904 ± 0.09	1.277GluPro: 1.277 ± 0.041	1.471GluGln: 1.471 ± 0.049	2.648GluArg: 2.648 ± 0.059	3.87GluSer: 3.87 ± 0.075	2.929GluThr: 2.929 ± 0.057	5.655GluVal: 5.655 ± 0.092	0.467GluTrp: 0.467 ± 0.023	3.181GluTyr: 3.181 ± 0.062	0.0GluXaa: 0.0 ± 0.0
Phe	2.145PheAla: 2.145 ± 0.047	0.467PheCys: 0.467 ± 0.023	2.4PheAsp: 2.4 ± 0.049	2.66PheGlu: 2.66 ± 0.062	1.953PhePhe: 1.953 ± 0.051	2.784PheGly: 2.784 ± 0.064	0.484PheHis: 0.484 ± 0.024	5.072PheIle: 5.072 ± 0.094	4.343PheLys: 4.343 ± 0.08	3.922PheLeu: 3.922 ± 0.077	1.154PheMet: 1.154 ± 0.038	3.332PheAsn: 3.332 ± 0.065	1.039PhePro: 1.039 ± 0.032	0.875PheGln: 0.875 ± 0.031	1.32PheArg: 1.32 ± 0.038	2.986PheSer: 2.986 ± 0.062	2.358PheThr: 2.358 ± 0.054	2.44PheVal: 2.44 ± 0.053	0.28PheTrp: 0.28 ± 0.02	1.788PheTyr: 1.788 ± 0.048	0.0PheXaa: 0.0 ± 0.0
Gly	3.895GlyAla: 3.895 ± 0.08	0.853GlyCys: 0.853 ± 0.04	3.47GlyAsp: 3.47 ± 0.059	4.763GlyGlu: 4.763 ± 0.083	3.128GlyPhe: 3.128 ± 0.065	4.147GlyGly: 4.147 ± 0.083	0.88GlyHis: 0.88 ± 0.031	6.766GlyIle: 6.766 ± 0.11	5.615GlyLys: 5.615 ± 0.087	5.419GlyLeu: 5.419 ± 0.095	1.647GlyMet: 1.647 ± 0.049	3.663GlyAsn: 3.663 ± 0.083	1.188GlyPro: 1.188 ± 0.04	1.363GlyGln: 1.363 ± 0.04	2.216GlyArg: 2.216 ± 0.052	3.545GlySer: 3.545 ± 0.065	3.317GlyThr: 3.317 ± 0.065	4.919GlyVal: 4.919 ± 0.083	0.486GlyTrp: 0.486 ± 0.026	2.986GlyTyr: 2.986 ± 0.068	0.0GlyXaa: 0.0 ± 0.0
His	0.591HisAla: 0.591 ± 0.025	0.17HisCys: 0.17 ± 0.014	0.597HisAsp: 0.597 ± 0.026	0.748HisGlu: 0.748 ± 0.028	0.566HisPhe: 0.566 ± 0.022	0.95HisGly: 0.95 ± 0.034	0.272HisHis: 0.272 ± 0.022	1.224HisIle: 1.224 ± 0.033	0.983HisLys: 0.983 ± 0.036	1.06HisLeu: 1.06 ± 0.035	0.339HisMet: 0.339 ± 0.021	0.774HisAsn: 0.774 ± 0.023	0.532HisPro: 0.532 ± 0.027	0.258HisGln: 0.258 ± 0.017	0.457HisArg: 0.457 ± 0.022	0.79HisSer: 0.79 ± 0.034	0.6HisThr: 0.6 ± 0.024	0.64HisVal: 0.64 ± 0.027	0.106HisTrp: 0.106 ± 0.012	0.532HisTyr: 0.532 ± 0.024	0.0HisXaa: 0.0 ± 0.0
Ile	5.574IleAla: 5.574 ± 0.1	1.147IleCys: 1.147 ± 0.04	6.144IleAsp: 6.144 ± 0.094	7.555IleGlu: 7.555 ± 0.103	4.486IlePhe: 4.486 ± 0.105	6.249IleGly: 6.249 ± 0.11	1.175IleHis: 1.175 ± 0.034	10.256IleIle: 10.256 ± 0.185	9.705IleLys: 9.705 ± 0.128	9.425IleLeu: 9.425 ± 0.135	2.277IleMet: 2.277 ± 0.051	7.264IleAsn: 7.264 ± 0.102	3.265IlePro: 3.265 ± 0.07	2.119IleGln: 2.119 ± 0.046	3.1IleArg: 3.1 ± 0.062	7.208IleSer: 7.208 ± 0.117	5.089IleThr: 5.089 ± 0.079	6.23IleVal: 6.23 ± 0.096	0.56IleTrp: 0.56 ± 0.026	3.887IleTyr: 3.887 ± 0.084	0.0IleXaa: 0.0 ± 0.0
Lys	4.815LysAla: 4.815 ± 0.078	0.869LysCys: 0.869 ± 0.033	6.185LysAsp: 6.185 ± 0.087	9.667LysGlu: 9.667 ± 0.121	3.45LysPhe: 3.45 ± 0.06	5.437LysGly: 5.437 ± 0.093	1.016LysHis: 1.016 ± 0.034	8.698LysIle: 8.698 ± 0.11	8.279LysLys: 8.279 ± 0.105	7.888LysLeu: 7.888 ± 0.083	2.232LysMet: 2.232 ± 0.054	6.859LysAsn: 6.859 ± 0.087	2.015LysPro: 2.015 ± 0.051	1.821LysGln: 1.821 ± 0.048	3.144LysArg: 3.144 ± 0.056	5.474LysSer: 5.474 ± 0.084	4.064LysThr: 4.064 ± 0.067	6.487LysVal: 6.487 ± 0.092	0.612LysTrp: 0.612 ± 0.03	4.233LysTyr: 4.233 ± 0.072	0.0LysXaa: 0.0 ± 0.0
Leu	5.259LeuAla: 5.259 ± 0.087	0.981LeuCys: 0.981 ± 0.037	5.313LeuAsp: 5.313 ± 0.078	6.811LeuGlu: 6.811 ± 0.093	3.605LeuPhe: 3.605 ± 0.084	6.207LeuGly: 6.207 ± 0.091	1.0LeuHis: 1.0 ± 0.035	8.583LeuIle: 8.583 ± 0.147	8.793LeuLys: 8.793 ± 0.113	7.777LeuLeu: 7.777 ± 0.125	2.221LeuMet: 2.221 ± 0.045	6.419LeuAsn: 6.419 ± 0.09	2.664LeuPro: 2.664 ± 0.058	2.029LeuGln: 2.029 ± 0.05	2.934LeuArg: 2.934 ± 0.059	6.454LeuSer: 6.454 ± 0.106	4.617LeuThr: 4.617 ± 0.061	5.547LeuVal: 5.547 ± 0.088	0.588LeuTrp: 0.588 ± 0.03	3.151LeuTyr: 3.151 ± 0.064	0.0LeuXaa: 0.0 ± 0.0
Met	1.68MetAla: 1.68 ± 0.044	0.234MetCys: 0.234 ± 0.018	1.446MetAsp: 1.446 ± 0.041	1.716MetGlu: 1.716 ± 0.042	1.008MetPhe: 1.008 ± 0.036	1.684MetGly: 1.684 ± 0.053	0.299MetHis: 0.299 ± 0.018	2.206MetIle: 2.206 ± 0.05	2.375MetLys: 2.375 ± 0.051	2.084MetLeu: 2.084 ± 0.057	0.608MetMet: 0.608 ± 0.028	1.535MetAsn: 1.535 ± 0.044	0.828MetPro: 0.828 ± 0.03	0.595MetGln: 0.595 ± 0.023	0.819MetArg: 0.819 ± 0.031	1.517MetSer: 1.517 ± 0.038	1.077MetThr: 1.077 ± 0.035	1.569MetVal: 1.569 ± 0.043	0.184MetTrp: 0.184 ± 0.015	0.874MetTyr: 0.874 ± 0.03	0.0MetXaa: 0.0 ± 0.0
Asn	2.783AsnAla: 2.783 ± 0.067	0.704AsnCys: 0.704 ± 0.029	3.326AsnAsp: 3.326 ± 0.068	4.994AsnGlu: 4.994 ± 0.085	2.827AsnPhe: 2.827 ± 0.054	4.259AsnGly: 4.259 ± 0.092	0.785AsnHis: 0.785 ± 0.033	8.002AsnIle: 8.002 ± 0.102	7.171AsnLys: 7.171 ± 0.093	6.366AsnLeu: 6.366 ± 0.1	1.571AsnMet: 1.571 ± 0.047	5.457AsnAsn: 5.457 ± 0.112	2.176AsnPro: 2.176 ± 0.057	1.351AsnGln: 1.351 ± 0.045	2.12AsnArg: 2.12 ± 0.05	4.508AsnSer: 4.508 ± 0.08	3.133AsnThr: 3.133 ± 0.065	3.707AsnVal: 3.707 ± 0.062	0.492AsnTrp: 0.492 ± 0.024	2.885AsnTyr: 2.885 ± 0.059	0.0AsnXaa: 0.0 ± 0.0
Pro	1.288ProAla: 1.288 ± 0.043	0.276ProCys: 0.276 ± 0.019	1.218ProAsp: 1.218 ± 0.038	2.066ProGlu: 2.066 ± 0.058	1.321ProPhe: 1.321 ± 0.037	1.694ProGly: 1.694 ± 0.046	0.432ProHis: 0.432 ± 0.024	2.718ProIle: 2.718 ± 0.058	2.221ProLys: 2.221 ± 0.051	2.349ProLeu: 2.349 ± 0.048	0.663ProMet: 0.663 ± 0.026	1.709ProAsn: 1.709 ± 0.048	0.527ProPro: 0.527 ± 0.029	0.758ProGln: 0.758 ± 0.029	0.777ProArg: 0.777 ± 0.033	1.727ProSer: 1.727 ± 0.047	1.438ProThr: 1.438 ± 0.04	1.848ProVal: 1.848 ± 0.046	0.234ProTrp: 0.234 ± 0.017	1.22ProTyr: 1.22 ± 0.04	0.0ProXaa: 0.0 ± 0.0
Gln	1.169GlnAla: 1.169 ± 0.039	0.244GlnCys: 0.244 ± 0.017	1.085GlnAsp: 1.085 ± 0.035	1.625GlnGlu: 1.625 ± 0.046	0.823GlnPhe: 0.823 ± 0.031	1.544GlnGly: 1.544 ± 0.041	0.248GlnHis: 0.248 ± 0.018	1.922GlnIle: 1.922 ± 0.045	1.765GlnLys: 1.765 ± 0.048	1.845GlnLeu: 1.845 ± 0.054	0.524GlnMet: 0.524 ± 0.027	1.319GlnAsn: 1.319 ± 0.034	0.54GlnPro: 0.54 ± 0.025	0.547GlnGln: 0.547 ± 0.028	0.824GlnArg: 0.824 ± 0.035	1.233GlnSer: 1.233 ± 0.041	0.912GlnThr: 0.912 ± 0.038	1.358GlnVal: 1.358 ± 0.042	0.201GlnTrp: 0.201 ± 0.017	0.954GlnTyr: 0.954 ± 0.033	0.0GlnXaa: 0.0 ± 0.0
Arg	1.761ArgAla: 1.761 ± 0.049	0.394ArgCys: 0.394 ± 0.024	1.916ArgAsp: 1.916 ± 0.048	2.934ArgGlu: 2.934 ± 0.056	1.446ArgPhe: 1.446 ± 0.041	2.059ArgGly: 2.059 ± 0.059	0.435ArgHis: 0.435 ± 0.021	3.088ArgIle: 3.088 ± 0.061	3.04ArgLys: 3.04 ± 0.057	2.823ArgLeu: 2.823 ± 0.059	0.888ArgMet: 0.888 ± 0.033	2.088ArgAsn: 2.088 ± 0.042	0.761ArgPro: 0.761 ± 0.03	0.766ArgGln: 0.766 ± 0.028	1.33ArgArg: 1.33 ± 0.045	1.618ArgSer: 1.618 ± 0.041	1.379ArgThr: 1.379 ± 0.037	2.371ArgVal: 2.371 ± 0.055	0.281ArgTrp: 0.281 ± 0.02	1.416ArgTyr: 1.416 ± 0.038	0.0ArgXaa: 0.0 ± 0.0
Ser	2.985SerAla: 2.985 ± 0.059	0.628SerCys: 0.628 ± 0.028	3.187SerAsp: 3.187 ± 0.061	4.201SerGlu: 4.201 ± 0.082	3.028SerPhe: 3.028 ± 0.063	4.214SerGly: 4.214 ± 0.075	0.83SerHis: 0.83 ± 0.027	6.887SerIle: 6.887 ± 0.096	6.001SerLys: 6.001 ± 0.077	6.15SerLeu: 6.15 ± 0.093	1.548SerMet: 1.548 ± 0.041	4.334SerAsn: 4.334 ± 0.075	1.573SerPro: 1.573 ± 0.04	1.374SerGln: 1.374 ± 0.036	1.961SerArg: 1.961 ± 0.052	4.031SerSer: 4.031 ± 0.082	3.226SerThr: 3.226 ± 0.062	3.565SerVal: 3.565 ± 0.061	0.444SerTrp: 0.444 ± 0.026	2.624SerTyr: 2.624 ± 0.062	0.0SerXaa: 0.0 ± 0.0
Thr	2.697ThrAla: 2.697 ± 0.054	0.48ThrCys: 0.48 ± 0.023	2.349ThrAsp: 2.349 ± 0.052	3.087ThrGlu: 3.087 ± 0.068	2.336ThrPhe: 2.336 ± 0.057	3.495ThrGly: 3.495 ± 0.061	0.675ThrHis: 0.675 ± 0.028	5.013ThrIle: 5.013 ± 0.076	3.746ThrLys: 3.746 ± 0.065	5.066ThrLeu: 5.066 ± 0.079	1.091ThrMet: 1.091 ± 0.032	3.005ThrAsn: 3.005 ± 0.068	1.793ThrPro: 1.793 ± 0.039	0.95ThrGln: 0.95 ± 0.033	1.524ThrArg: 1.524 ± 0.044	3.153ThrSer: 3.153 ± 0.059	2.65ThrThr: 2.65 ± 0.06	3.237ThrVal: 3.237 ± 0.063	0.382ThrTrp: 0.382 ± 0.025	1.855ThrTyr: 1.855 ± 0.054	0.0ThrXaa: 0.0 ± 0.0
Val	3.813ValAla: 3.813 ± 0.074	0.746ValCys: 0.746 ± 0.03	3.96ValAsp: 3.96 ± 0.067	4.614ValGlu: 4.614 ± 0.076	2.743ValPhe: 2.743 ± 0.058	4.136ValGly: 4.136 ± 0.07	0.756ValHis: 0.756 ± 0.03	6.385ValIle: 6.385 ± 0.091	5.662ValLys: 5.662 ± 0.087	6.008ValLeu: 6.008 ± 0.082	1.425ValMet: 1.425 ± 0.042	4.241ValAsn: 4.241 ± 0.078	1.816ValPro: 1.816 ± 0.043	1.329ValGln: 1.329 ± 0.035	2.033ValArg: 2.033 ± 0.051	4.237ValSer: 4.237 ± 0.063	3.332ValThr: 3.332 ± 0.065	4.538ValVal: 4.538 ± 0.072	0.345ValTrp: 0.345 ± 0.019	2.319ValTyr: 2.319 ± 0.055	0.0ValXaa: 0.0 ± 0.0
Trp	0.381TrpAla: 0.381 ± 0.023	0.087TrpCys: 0.087 ± 0.01	0.41TrpAsp: 0.41 ± 0.021	0.434TrpGlu: 0.434 ± 0.023	0.316TrpPhe: 0.316 ± 0.023	0.478TrpGly: 0.478 ± 0.026	0.131TrpHis: 0.131 ± 0.011	0.689TrpIle: 0.689 ± 0.03	0.528TrpLys: 0.528 ± 0.024	0.543TrpLeu: 0.543 ± 0.028	0.169TrpMet: 0.169 ± 0.014	0.487TrpAsn: 0.487 ± 0.03	0.149TrpPro: 0.149 ± 0.012	0.194TrpGln: 0.194 ± 0.014	0.252TrpArg: 0.252 ± 0.018	0.421TrpSer: 0.421 ± 0.023	0.347TrpThr: 0.347 ± 0.023	0.429TrpVal: 0.429 ± 0.025	0.073TrpTrp: 0.073 ± 0.01	0.281TrpTyr: 0.281 ± 0.022	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.807TyrAla: 1.807 ± 0.044	0.48TyrCys: 0.48 ± 0.025	2.294TyrAsp: 2.294 ± 0.061	2.892TyrGlu: 2.892 ± 0.059	1.923TyrPhe: 1.923 ± 0.053	2.589TyrGly: 2.589 ± 0.055	0.492TyrHis: 0.492 ± 0.022	4.205TyrIle: 4.205 ± 0.082	3.903TyrLys: 3.903 ± 0.074	3.686TyrLeu: 3.686 ± 0.076	0.93TyrMet: 0.93 ± 0.029	3.067TyrAsn: 3.067 ± 0.064	1.226TyrPro: 1.226 ± 0.04	0.786TyrGln: 0.786 ± 0.032	1.463TyrArg: 1.463 ± 0.039	2.811TyrSer: 2.811 ± 0.065	2.107TyrThr: 2.107 ± 0.051	2.226TyrVal: 2.226 ± 0.049	0.283TyrTrp: 0.283 ± 0.02	1.892TyrTyr: 1.892 ± 0.052	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2908 proteins (919036 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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