Amino acid dipepetide frequency for Microbacterium phage DizzyRudy

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.254AlaAla: 9.254 ± 1.823
0.058AlaCys: 0.058 ± 0.059
5.495AlaAsp: 5.495 ± 0.662
6.709AlaGlu: 6.709 ± 0.764
3.47AlaPhe: 3.47 ± 0.433
7.345AlaGly: 7.345 ± 1.433
1.215AlaHis: 1.215 ± 0.249
6.131AlaIle: 6.131 ± 0.868
5.09AlaLys: 5.09 ± 0.797
7.056AlaLeu: 7.056 ± 1.012
3.008AlaMet: 3.008 ± 0.84
3.933AlaAsn: 3.933 ± 0.568
2.66AlaPro: 2.66 ± 0.429
3.239AlaGln: 3.239 ± 0.45
5.032AlaArg: 5.032 ± 0.729
5.495AlaSer: 5.495 ± 0.871
5.784AlaThr: 5.784 ± 0.584
7.287AlaVal: 7.287 ± 0.997
1.619AlaTrp: 1.619 ± 0.316
3.181AlaTyr: 3.181 ± 0.498
0.0AlaXaa: 0.0 ± 0.0
Cys
0.289CysAla: 0.289 ± 0.147
0.0CysCys: 0.0 ± 0.0
0.463CysAsp: 0.463 ± 0.182
0.289CysGlu: 0.289 ± 0.116
0.116CysPhe: 0.116 ± 0.088
0.463CysGly: 0.463 ± 0.175
0.116CysHis: 0.116 ± 0.08
0.347CysIle: 0.347 ± 0.154
0.347CysLys: 0.347 ± 0.137
0.347CysLeu: 0.347 ± 0.147
0.231CysMet: 0.231 ± 0.107
0.116CysAsn: 0.116 ± 0.074
0.289CysPro: 0.289 ± 0.143
0.116CysGln: 0.116 ± 0.087
0.289CysArg: 0.289 ± 0.154
0.347CysSer: 0.347 ± 0.144
0.116CysThr: 0.116 ± 0.079
0.347CysVal: 0.347 ± 0.201
0.116CysTrp: 0.116 ± 0.078
0.0CysTyr: 0.0 ± 0.0
0.0CysXaa: 0.0 ± 0.0
Asp
5.842AspAla: 5.842 ± 0.605
0.463AspCys: 0.463 ± 0.171
5.032AspAsp: 5.032 ± 0.615
4.858AspGlu: 4.858 ± 0.661
2.313AspPhe: 2.313 ± 0.542
5.321AspGly: 5.321 ± 0.584
0.983AspHis: 0.983 ± 0.282
4.627AspIle: 4.627 ± 0.688
3.47AspLys: 3.47 ± 0.432
5.668AspLeu: 5.668 ± 0.555
1.677AspMet: 1.677 ± 0.426
3.008AspAsn: 3.008 ± 0.438
3.644AspPro: 3.644 ± 0.516
2.14AspGln: 2.14 ± 0.351
2.834AspArg: 2.834 ± 0.543
2.834AspSer: 2.834 ± 0.386
2.892AspThr: 2.892 ± 0.322
3.181AspVal: 3.181 ± 0.443
0.694AspTrp: 0.694 ± 0.175
2.487AspTyr: 2.487 ± 0.361
0.0AspXaa: 0.0 ± 0.0
Glu
6.478GluAla: 6.478 ± 0.741
0.0GluCys: 0.0 ± 0.0
3.991GluAsp: 3.991 ± 0.654
5.726GluGlu: 5.726 ± 0.672
3.008GluPhe: 3.008 ± 0.337
4.049GluGly: 4.049 ± 0.511
0.925GluHis: 0.925 ± 0.236
4.164GluIle: 4.164 ± 0.447
4.685GluLys: 4.685 ± 0.745
6.073GluLeu: 6.073 ± 0.613
2.024GluMet: 2.024 ± 0.383
2.95GluAsn: 2.95 ± 0.442
2.313GluPro: 2.313 ± 0.509
2.776GluGln: 2.776 ± 0.442
3.933GluArg: 3.933 ± 0.515
2.834GluSer: 2.834 ± 0.373
4.743GluThr: 4.743 ± 0.57
5.379GluVal: 5.379 ± 0.658
1.504GluTrp: 1.504 ± 0.305
2.14GluTyr: 2.14 ± 0.372
0.0GluXaa: 0.0 ± 0.0
Phe
2.718PheAla: 2.718 ± 0.465
0.521PheCys: 0.521 ± 0.162
3.065PheAsp: 3.065 ± 0.391
3.123PheGlu: 3.123 ± 0.506
1.388PhePhe: 1.388 ± 0.223
3.355PheGly: 3.355 ± 0.345
0.578PheHis: 0.578 ± 0.179
1.909PheIle: 1.909 ± 0.328
1.677PheLys: 1.677 ± 0.342
2.834PheLeu: 2.834 ± 0.311
0.694PheMet: 0.694 ± 0.213
1.909PheAsn: 1.909 ± 0.386
1.33PhePro: 1.33 ± 0.27
0.868PheGln: 0.868 ± 0.206
2.198PheArg: 2.198 ± 0.418
2.371PheSer: 2.371 ± 0.422
2.024PheThr: 2.024 ± 0.388
2.95PheVal: 2.95 ± 0.503
0.752PheTrp: 0.752 ± 0.328
1.388PheTyr: 1.388 ± 0.252
0.0PheXaa: 0.0 ± 0.0
Gly
7.461GlyAla: 7.461 ± 1.576
0.289GlyCys: 0.289 ± 0.13
5.784GlyAsp: 5.784 ± 0.428
4.685GlyGlu: 4.685 ± 0.464
3.065GlyPhe: 3.065 ± 0.44
6.94GlyGly: 6.94 ± 1.833
1.099GlyHis: 1.099 ± 0.289
4.8GlyIle: 4.8 ± 0.824
3.528GlyLys: 3.528 ± 0.461
6.131GlyLeu: 6.131 ± 1.023
1.735GlyMet: 1.735 ± 0.409
2.603GlyAsn: 2.603 ± 0.433
2.256GlyPro: 2.256 ± 0.432
2.371GlyGln: 2.371 ± 0.345
3.817GlyArg: 3.817 ± 0.47
4.569GlySer: 4.569 ± 0.68
5.784GlyThr: 5.784 ± 0.754
5.437GlyVal: 5.437 ± 0.579
1.272GlyTrp: 1.272 ± 0.268
2.95GlyTyr: 2.95 ± 0.433
0.0GlyXaa: 0.0 ± 0.0
His
1.504HisAla: 1.504 ± 0.318
0.116HisCys: 0.116 ± 0.07
1.272HisAsp: 1.272 ± 0.309
1.215HisGlu: 1.215 ± 0.31
0.636HisPhe: 0.636 ± 0.201
1.388HisGly: 1.388 ± 0.343
0.289HisHis: 0.289 ± 0.117
1.33HisIle: 1.33 ± 0.365
1.33HisLys: 1.33 ± 0.256
1.504HisLeu: 1.504 ± 0.351
0.463HisMet: 0.463 ± 0.226
1.041HisAsn: 1.041 ± 0.308
1.041HisPro: 1.041 ± 0.249
0.868HisGln: 0.868 ± 0.236
0.636HisArg: 0.636 ± 0.205
1.215HisSer: 1.215 ± 0.286
0.636HisThr: 0.636 ± 0.201
0.983HisVal: 0.983 ± 0.269
0.405HisTrp: 0.405 ± 0.168
0.983HisTyr: 0.983 ± 0.251
0.0HisXaa: 0.0 ± 0.0
Ile
5.032IleAla: 5.032 ± 0.55
0.289IleCys: 0.289 ± 0.129
4.164IleAsp: 4.164 ± 0.562
3.933IleGlu: 3.933 ± 0.524
2.313IlePhe: 2.313 ± 0.37
3.644IleGly: 3.644 ± 0.992
1.215IleHis: 1.215 ± 0.305
2.66IleIle: 2.66 ± 0.428
4.164IleLys: 4.164 ± 0.688
4.974IleLeu: 4.974 ± 0.633
1.446IleMet: 1.446 ± 0.371
2.256IleAsn: 2.256 ± 0.31
2.834IlePro: 2.834 ± 0.378
1.677IleGln: 1.677 ± 0.243
3.181IleArg: 3.181 ± 0.462
3.181IleSer: 3.181 ± 0.467
3.355IleThr: 3.355 ± 0.545
4.569IleVal: 4.569 ± 0.547
0.752IleTrp: 0.752 ± 0.218
1.966IleTyr: 1.966 ± 0.352
0.0IleXaa: 0.0 ± 0.0
Lys
5.899LysAla: 5.899 ± 0.797
0.231LysCys: 0.231 ± 0.108
2.834LysAsp: 2.834 ± 0.444
3.528LysGlu: 3.528 ± 0.473
1.909LysPhe: 1.909 ± 0.39
3.47LysGly: 3.47 ± 0.519
1.793LysHis: 1.793 ± 0.331
3.644LysIle: 3.644 ± 0.507
4.28LysLys: 4.28 ± 0.771
5.032LysLeu: 5.032 ± 0.712
2.14LysMet: 2.14 ± 0.428
3.239LysAsn: 3.239 ± 0.418
2.256LysPro: 2.256 ± 0.394
1.966LysGln: 1.966 ± 0.458
2.603LysArg: 2.603 ± 0.509
3.412LysSer: 3.412 ± 0.593
3.702LysThr: 3.702 ± 0.41
4.106LysVal: 4.106 ± 0.422
0.868LysTrp: 0.868 ± 0.203
2.082LysTyr: 2.082 ± 0.488
0.0LysXaa: 0.0 ± 0.0
Leu
7.634LeuAla: 7.634 ± 1.127
0.116LeuCys: 0.116 ± 0.085
4.916LeuAsp: 4.916 ± 0.591
5.61LeuGlu: 5.61 ± 0.495
3.065LeuPhe: 3.065 ± 0.348
5.899LeuGly: 5.899 ± 0.706
1.157LeuHis: 1.157 ± 0.293
3.759LeuIle: 3.759 ± 0.712
5.437LeuLys: 5.437 ± 0.743
4.974LeuLeu: 4.974 ± 0.727
2.603LeuMet: 2.603 ± 0.429
3.181LeuAsn: 3.181 ± 0.424
3.355LeuPro: 3.355 ± 0.489
2.313LeuGln: 2.313 ± 0.326
4.106LeuArg: 4.106 ± 0.698
4.916LeuSer: 4.916 ± 0.653
5.495LeuThr: 5.495 ± 0.611
6.362LeuVal: 6.362 ± 0.508
0.752LeuTrp: 0.752 ± 0.202
2.66LeuTyr: 2.66 ± 0.548
0.0LeuXaa: 0.0 ± 0.0
Met
2.371MetAla: 2.371 ± 0.444
0.116MetCys: 0.116 ± 0.08
1.619MetAsp: 1.619 ± 0.303
1.735MetGlu: 1.735 ± 0.25
0.925MetPhe: 0.925 ± 0.221
1.966MetGly: 1.966 ± 0.509
0.347MetHis: 0.347 ± 0.152
1.504MetIle: 1.504 ± 0.367
1.215MetLys: 1.215 ± 0.302
2.082MetLeu: 2.082 ± 0.308
0.636MetMet: 0.636 ± 0.245
0.983MetAsn: 0.983 ± 0.218
1.215MetPro: 1.215 ± 0.21
1.099MetGln: 1.099 ± 0.307
1.619MetArg: 1.619 ± 0.364
2.603MetSer: 2.603 ± 0.564
1.966MetThr: 1.966 ± 0.371
1.909MetVal: 1.909 ± 0.404
0.174MetTrp: 0.174 ± 0.092
0.925MetTyr: 0.925 ± 0.197
0.0MetXaa: 0.0 ± 0.0
Asn
4.106AsnAla: 4.106 ± 0.692
0.289AsnCys: 0.289 ± 0.133
2.603AsnAsp: 2.603 ± 0.444
2.718AsnGlu: 2.718 ± 0.414
1.851AsnPhe: 1.851 ± 0.312
3.875AsnGly: 3.875 ± 0.505
1.157AsnHis: 1.157 ± 0.28
2.256AsnIle: 2.256 ± 0.379
1.966AsnLys: 1.966 ± 0.308
3.239AsnLeu: 3.239 ± 0.455
1.099AsnMet: 1.099 ± 0.274
1.966AsnAsn: 1.966 ± 0.26
2.256AsnPro: 2.256 ± 0.381
1.157AsnGln: 1.157 ± 0.206
2.776AsnArg: 2.776 ± 0.5
3.239AsnSer: 3.239 ± 0.445
1.909AsnThr: 1.909 ± 0.323
2.776AsnVal: 2.776 ± 0.368
0.752AsnTrp: 0.752 ± 0.231
1.388AsnTyr: 1.388 ± 0.353
0.0AsnXaa: 0.0 ± 0.0
Pro
4.338ProAla: 4.338 ± 0.597
0.347ProCys: 0.347 ± 0.139
2.545ProAsp: 2.545 ± 0.395
3.008ProGlu: 3.008 ± 0.503
1.446ProPhe: 1.446 ± 0.284
3.008ProGly: 3.008 ± 0.469
0.868ProHis: 0.868 ± 0.28
1.909ProIle: 1.909 ± 0.402
2.603ProLys: 2.603 ± 0.437
2.66ProLeu: 2.66 ± 0.49
1.272ProMet: 1.272 ± 0.266
1.677ProAsn: 1.677 ± 0.329
1.446ProPro: 1.446 ± 0.291
1.099ProGln: 1.099 ± 0.251
3.297ProArg: 3.297 ± 0.549
2.313ProSer: 2.313 ± 0.381
2.776ProThr: 2.776 ± 0.494
2.892ProVal: 2.892 ± 0.446
0.521ProTrp: 0.521 ± 0.17
1.33ProTyr: 1.33 ± 0.304
0.0ProXaa: 0.0 ± 0.0
Gln
3.875GlnAla: 3.875 ± 0.503
0.116GlnCys: 0.116 ± 0.078
1.157GlnAsp: 1.157 ± 0.272
2.024GlnGlu: 2.024 ± 0.401
0.868GlnPhe: 0.868 ± 0.207
2.256GlnGly: 2.256 ± 0.392
0.983GlnHis: 0.983 ± 0.266
2.198GlnIle: 2.198 ± 0.392
2.256GlnLys: 2.256 ± 0.403
2.603GlnLeu: 2.603 ± 0.521
0.925GlnMet: 0.925 ± 0.236
1.272GlnAsn: 1.272 ± 0.243
1.446GlnPro: 1.446 ± 0.362
1.215GlnGln: 1.215 ± 0.291
2.313GlnArg: 2.313 ± 0.373
1.735GlnSer: 1.735 ± 0.334
1.909GlnThr: 1.909 ± 0.335
1.966GlnVal: 1.966 ± 0.374
0.578GlnTrp: 0.578 ± 0.152
0.868GlnTyr: 0.868 ± 0.218
0.0GlnXaa: 0.0 ± 0.0
Arg
4.049ArgAla: 4.049 ± 0.483
0.231ArgCys: 0.231 ± 0.117
3.875ArgAsp: 3.875 ± 0.605
3.412ArgGlu: 3.412 ± 0.485
2.371ArgPhe: 2.371 ± 0.461
3.702ArgGly: 3.702 ± 0.461
1.677ArgHis: 1.677 ± 0.435
1.966ArgIle: 1.966 ± 0.325
3.759ArgLys: 3.759 ± 0.525
4.974ArgLeu: 4.974 ± 0.618
1.388ArgMet: 1.388 ± 0.285
2.718ArgAsn: 2.718 ± 0.479
2.14ArgPro: 2.14 ± 0.36
1.272ArgGln: 1.272 ± 0.276
2.95ArgArg: 2.95 ± 0.423
3.412ArgSer: 3.412 ± 0.461
4.222ArgThr: 4.222 ± 0.564
3.412ArgVal: 3.412 ± 0.525
0.925ArgTrp: 0.925 ± 0.248
1.966ArgTyr: 1.966 ± 0.411
0.0ArgXaa: 0.0 ± 0.0
Ser
5.321SerAla: 5.321 ± 0.762
0.289SerCys: 0.289 ± 0.157
2.545SerAsp: 2.545 ± 0.373
4.106SerGlu: 4.106 ± 0.446
2.603SerPhe: 2.603 ± 0.363
5.899SerGly: 5.899 ± 0.667
0.752SerHis: 0.752 ± 0.223
3.528SerIle: 3.528 ± 0.442
3.817SerLys: 3.817 ± 0.682
4.974SerLeu: 4.974 ± 0.928
1.562SerMet: 1.562 ± 0.36
2.198SerAsn: 2.198 ± 0.445
2.487SerPro: 2.487 ± 0.381
2.14SerGln: 2.14 ± 0.282
2.545SerArg: 2.545 ± 0.415
4.396SerSer: 4.396 ± 0.831
3.355SerThr: 3.355 ± 0.512
3.528SerVal: 3.528 ± 0.461
1.272SerTrp: 1.272 ± 0.275
2.256SerTyr: 2.256 ± 0.41
0.0SerXaa: 0.0 ± 0.0
Thr
6.015ThrAla: 6.015 ± 0.777
0.174ThrCys: 0.174 ± 0.112
3.47ThrAsp: 3.47 ± 0.512
3.991ThrGlu: 3.991 ± 0.52
2.024ThrPhe: 2.024 ± 0.305
5.379ThrGly: 5.379 ± 0.63
1.157ThrHis: 1.157 ± 0.37
3.759ThrIle: 3.759 ± 0.485
3.239ThrLys: 3.239 ± 0.477
5.437ThrLeu: 5.437 ± 0.485
1.272ThrMet: 1.272 ± 0.374
2.371ThrAsn: 2.371 ± 0.532
3.47ThrPro: 3.47 ± 0.418
2.082ThrGln: 2.082 ± 0.401
3.297ThrArg: 3.297 ± 0.482
3.586ThrSer: 3.586 ± 0.528
3.759ThrThr: 3.759 ± 0.553
4.338ThrVal: 4.338 ± 0.542
0.81ThrTrp: 0.81 ± 0.233
1.562ThrTyr: 1.562 ± 0.262
0.0ThrXaa: 0.0 ± 0.0
Val
6.883ValAla: 6.883 ± 0.573
0.521ValCys: 0.521 ± 0.229
5.032ValAsp: 5.032 ± 0.553
5.321ValGlu: 5.321 ± 0.794
2.313ValPhe: 2.313 ± 0.334
4.974ValGly: 4.974 ± 0.687
1.157ValHis: 1.157 ± 0.284
4.453ValIle: 4.453 ± 0.412
3.991ValLys: 3.991 ± 0.476
4.338ValLeu: 4.338 ± 0.468
1.272ValMet: 1.272 ± 0.224
3.644ValAsn: 3.644 ± 0.482
3.355ValPro: 3.355 ± 0.45
2.487ValGln: 2.487 ± 0.349
3.528ValArg: 3.528 ± 0.431
4.28ValSer: 4.28 ± 0.613
3.528ValThr: 3.528 ± 0.481
5.784ValVal: 5.784 ± 0.638
1.041ValTrp: 1.041 ± 0.231
2.718ValTyr: 2.718 ± 0.509
0.0ValXaa: 0.0 ± 0.0
Trp
1.504TrpAla: 1.504 ± 0.272
0.116TrpCys: 0.116 ± 0.072
1.33TrpAsp: 1.33 ± 0.328
1.157TrpGlu: 1.157 ± 0.267
0.868TrpPhe: 0.868 ± 0.253
1.272TrpGly: 1.272 ± 0.317
0.289TrpHis: 0.289 ± 0.133
0.694TrpIle: 0.694 ± 0.21
0.405TrpLys: 0.405 ± 0.189
0.925TrpLeu: 0.925 ± 0.261
0.81TrpMet: 0.81 ± 0.203
0.868TrpAsn: 0.868 ± 0.235
0.174TrpPro: 0.174 ± 0.101
0.231TrpGln: 0.231 ± 0.118
0.925TrpArg: 0.925 ± 0.22
0.752TrpSer: 0.752 ± 0.208
1.215TrpThr: 1.215 ± 0.252
1.041TrpVal: 1.041 ± 0.259
0.174TrpTrp: 0.174 ± 0.097
0.694TrpTyr: 0.694 ± 0.2
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.487TyrAla: 2.487 ± 0.382
0.463TyrCys: 0.463 ± 0.191
2.776TyrAsp: 2.776 ± 0.514
2.545TyrGlu: 2.545 ± 0.456
1.099TyrPhe: 1.099 ± 0.269
2.371TyrGly: 2.371 ± 0.39
1.099TyrHis: 1.099 ± 0.262
1.966TyrIle: 1.966 ± 0.372
1.735TyrLys: 1.735 ± 0.458
2.487TyrLeu: 2.487 ± 0.412
0.694TyrMet: 0.694 ± 0.209
1.504TyrAsn: 1.504 ± 0.279
1.388TyrPro: 1.388 ± 0.293
1.446TyrGln: 1.446 ± 0.256
2.545TyrArg: 2.545 ± 0.5
2.082TyrSer: 2.082 ± 0.427
2.024TyrThr: 2.024 ± 0.392
2.371TyrVal: 2.371 ± 0.413
0.463TyrTrp: 0.463 ± 0.187
1.446TyrTyr: 1.446 ± 0.341
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 86 proteins (17291 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski