Tortoise microvirus 89

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 7.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P8W6X0|A0A4P8W6X0_9VIRU Replication initiation protein OS=Tortoise microvirus 89 OX=2583197 PE=4 SV=1
MM1 pKa = 7.08EE2 pKa = 5.08RR3 pKa = 11.84KK4 pKa = 9.07VRR6 pKa = 11.84NWLNNHH12 pKa = 5.21EE13 pKa = 4.14FLYY16 pKa = 10.81VGEE19 pKa = 4.36VNDD22 pKa = 4.49MPSLTKK28 pKa = 10.19PDD30 pKa = 3.68DD31 pKa = 3.64TLSISEE37 pKa = 3.92ILDD40 pKa = 3.51RR41 pKa = 11.84YY42 pKa = 10.82SRR44 pKa = 11.84GLPLTGMKK52 pKa = 10.32VPVFDD57 pKa = 5.46EE58 pKa = 4.52YY59 pKa = 11.63DD60 pKa = 3.51DD61 pKa = 4.28TPDD64 pKa = 4.21IRR66 pKa = 11.84TLDD69 pKa = 3.37LSEE72 pKa = 5.63RR73 pKa = 11.84FDD75 pKa = 3.64LTAEE79 pKa = 3.83FHH81 pKa = 6.98EE82 pKa = 4.35EE83 pKa = 3.65LKK85 pKa = 10.63EE86 pKa = 3.93IRR88 pKa = 11.84SNAARR93 pKa = 11.84RR94 pKa = 11.84RR95 pKa = 11.84AEE97 pKa = 4.0RR98 pKa = 11.84EE99 pKa = 3.69AKK101 pKa = 9.97LAAEE105 pKa = 4.24RR106 pKa = 11.84EE107 pKa = 4.18EE108 pKa = 4.04MEE110 pKa = 5.08LIRR113 pKa = 11.84KK114 pKa = 7.55EE115 pKa = 3.83VQKK118 pKa = 10.61RR119 pKa = 11.84KK120 pKa = 10.24KK121 pKa = 10.08EE122 pKa = 3.75ADD124 pKa = 3.26EE125 pKa = 5.17AEE127 pKa = 4.32NSTNPSS133 pKa = 2.99

Molecular weight: 15.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P8W791|A0A4P8W791_9VIRU Replication initiation protein OS=Tortoise microvirus 89 OX=2583197 PE=4 SV=1
MM1 pKa = 7.76TIRR4 pKa = 11.84NKK6 pKa = 8.83VTGEE10 pKa = 3.88WNTFPCGKK18 pKa = 10.05CPEE21 pKa = 4.29CLARR25 pKa = 11.84RR26 pKa = 11.84TSGWSFRR33 pKa = 11.84LMEE36 pKa = 4.08EE37 pKa = 4.11SKK39 pKa = 10.51RR40 pKa = 11.84AEE42 pKa = 4.04SAHH45 pKa = 5.99FVTLTYY51 pKa = 9.82ATAPVTKK58 pKa = 9.76KK59 pKa = 11.03GFMTLCKK66 pKa = 9.99KK67 pKa = 10.32DD68 pKa = 3.32VQDD71 pKa = 3.49FFKK74 pKa = 10.82RR75 pKa = 11.84LRR77 pKa = 11.84YY78 pKa = 9.82YY79 pKa = 10.52LGKK82 pKa = 10.07DD83 pKa = 3.3VKK85 pKa = 10.26IKK87 pKa = 10.06YY88 pKa = 9.19YY89 pKa = 10.84VVGEE93 pKa = 4.1YY94 pKa = 8.36GTRR97 pKa = 11.84TLRR100 pKa = 11.84PHH102 pKa = 4.94YY103 pKa = 9.95HH104 pKa = 6.89AIIFGANYY112 pKa = 10.38YY113 pKa = 10.45GIEE116 pKa = 4.28KK117 pKa = 10.24AWSLDD122 pKa = 3.11NKK124 pKa = 10.63RR125 pKa = 11.84IGLVHH130 pKa = 6.1YY131 pKa = 9.5GVVNGASVGYY141 pKa = 6.39TLKK144 pKa = 11.06YY145 pKa = 8.55MSKK148 pKa = 9.15PSKK151 pKa = 10.51IPLHH155 pKa = 6.56KK156 pKa = 10.67NDD158 pKa = 4.21DD159 pKa = 3.81RR160 pKa = 11.84VKK162 pKa = 10.41EE163 pKa = 4.04FSLMSKK169 pKa = 10.41GLAATISHH177 pKa = 7.27LGCC180 pKa = 4.7

Molecular weight: 20.57 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1236

95

509

247.2

28.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.9 ± 1.813

0.89 ± 0.397

6.149 ± 0.79

6.796 ± 1.675

3.722 ± 0.621

6.472 ± 1.05

2.104 ± 0.314

5.421 ± 0.468

6.472 ± 1.583

7.282 ± 0.901

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.236 ± 0.169

4.935 ± 0.438

4.45 ± 1.081

3.883 ± 1.064

7.039 ± 0.993

5.097 ± 0.684

5.421 ± 0.776

5.502 ± 0.918

1.375 ± 0.205

4.854 ± 0.937
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski