Mycobacterium phage Donkeykong

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Cheoctovirus; unclassified Cheoctovirus

Average proteome isoelectric point is 5.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 110 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A649VEU4|A0A649VEU4_9CAUD Uncharacterized protein OS=Mycobacterium phage Donkeykong OX=2656572 GN=59 PE=4 SV=1
MM1 pKa = 7.19NASEE5 pKa = 5.76DD6 pKa = 3.69GLEE9 pKa = 4.36PLGEE13 pKa = 4.45APDD16 pKa = 4.44LGAPHH21 pKa = 6.8YY22 pKa = 10.26PMSAEE27 pKa = 4.24VTFHH31 pKa = 7.62RR32 pKa = 11.84ARR34 pKa = 11.84CTKK37 pKa = 10.5CGDD40 pKa = 3.08IEE42 pKa = 4.31TDD44 pKa = 3.37YY45 pKa = 11.69GDD47 pKa = 3.98FSAYY51 pKa = 10.02VDD53 pKa = 3.44PGGAINAVLAAADD66 pKa = 3.49WFGRR70 pKa = 11.84SAPTGEE76 pKa = 5.08LIDD79 pKa = 4.08FGGGRR84 pKa = 11.84MGQRR88 pKa = 11.84YY89 pKa = 7.81QLVEE93 pKa = 4.45LLCPDD98 pKa = 3.6CRR100 pKa = 11.84RR101 pKa = 11.84CEE103 pKa = 3.99VCGTAKK109 pKa = 10.44AYY111 pKa = 9.85PIDD114 pKa = 3.85DD115 pKa = 4.0HH116 pKa = 6.93LVCEE120 pKa = 4.29DD121 pKa = 4.12HH122 pKa = 7.29EE123 pKa = 4.31DD124 pKa = 3.94HH125 pKa = 6.79EE126 pKa = 5.25FEE128 pKa = 4.96AAPP131 pKa = 3.95

Molecular weight: 14.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A649VFJ3|A0A649VFJ3_9CAUD Uncharacterized protein OS=Mycobacterium phage Donkeykong OX=2656572 GN=47 PE=4 SV=1
MM1 pKa = 7.43TFFSGRR7 pKa = 11.84RR8 pKa = 11.84GSARR12 pKa = 11.84HH13 pKa = 5.13GKK15 pKa = 8.78GVGMARR21 pKa = 11.84RR22 pKa = 11.84GMARR26 pKa = 11.84LGTTWQAWLGMARR39 pKa = 11.84LGWAWLGAARR49 pKa = 11.84HH50 pKa = 5.89GRR52 pKa = 11.84HH53 pKa = 6.43DD54 pKa = 3.43MAGTARR60 pKa = 11.84RR61 pKa = 11.84GKK63 pKa = 10.47ARR65 pKa = 11.84LGTARR70 pKa = 11.84RR71 pKa = 11.84GKK73 pKa = 10.17ARR75 pKa = 11.84QAGQGQARR83 pKa = 11.84RR84 pKa = 11.84GRR86 pKa = 11.84ARR88 pKa = 11.84QGKK91 pKa = 8.43AWQARR96 pKa = 11.84LGEE99 pKa = 4.27ARR101 pKa = 11.84PGEE104 pKa = 4.35AGQGQARR111 pKa = 11.84RR112 pKa = 11.84GRR114 pKa = 11.84NGGAWLGNQPTQKK127 pKa = 10.79GG128 pKa = 3.44

Molecular weight: 13.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

110

0

110

19092

28

1226

173.6

19.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.827 ± 0.452

1.294 ± 0.191

6.767 ± 0.225

6.076 ± 0.341

2.876 ± 0.208

8.805 ± 0.584

2.247 ± 0.188

4.4 ± 0.208

3.295 ± 0.181

7.391 ± 0.198

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.331 ± 0.127

3.185 ± 0.157

5.966 ± 0.191

3.352 ± 0.205

7.129 ± 0.428

5.709 ± 0.308

6.552 ± 0.229

7.05 ± 0.259

2.305 ± 0.14

2.446 ± 0.149
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski