Desulfovibrio gigas (strain ATCC 19364 / DSM 1382 / NCIMB 9332 / VKM B-1759)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; Desulfovibrionales; Desulfovibrionaceae; Desulfovibrio; Desulfovibrio gigas

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3321 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|T2GAH1|T2GAH1_DESGG DD-transpeptidase OS=Desulfovibrio gigas (strain ATCC 19364 / DSM 1382 / NCIMB 9332 / VKM B-1759) OX=1121448 GN=DGI_1323 PE=3 SV=1
MM1 pKa = 7.86PLNIPDD7 pKa = 4.61LDD9 pKa = 3.43IDD11 pKa = 3.92YY12 pKa = 11.31GIITSGACATPGDD25 pKa = 3.91QAQFDD30 pKa = 4.0AHH32 pKa = 6.95QMGKK36 pKa = 9.61PPMAEE41 pKa = 3.93DD42 pKa = 3.26

Molecular weight: 4.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|T2GEK6|T2GEK6_DESGG Uncharacterized protein OS=Desulfovibrio gigas (strain ATCC 19364 / DSM 1382 / NCIMB 9332 / VKM B-1759) OX=1121448 GN=DGI_3347 PE=4 SV=1
MM1 pKa = 6.81AQKK4 pKa = 8.03MTYY7 pKa = 9.36QPHH10 pKa = 4.12TTRR13 pKa = 11.84RR14 pKa = 11.84KK15 pKa = 7.56RR16 pKa = 11.84THH18 pKa = 5.88GFLVRR23 pKa = 11.84SRR25 pKa = 11.84TKK27 pKa = 10.46NGQAVLRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84AKK39 pKa = 9.86GRR41 pKa = 11.84KK42 pKa = 8.77RR43 pKa = 11.84LAAA46 pKa = 4.42

Molecular weight: 5.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3321

0

3321

1099738

33

2870

331.1

36.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.972 ± 0.061

1.441 ± 0.018

5.234 ± 0.028

6.002 ± 0.04

3.639 ± 0.029

7.849 ± 0.045

2.406 ± 0.023

4.327 ± 0.032

3.458 ± 0.039

11.499 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.681 ± 0.017

2.415 ± 0.022

5.471 ± 0.04

3.98 ± 0.027

6.825 ± 0.04

4.927 ± 0.024

5.055 ± 0.029

7.309 ± 0.036

1.275 ± 0.019

2.236 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski