Thalassobius gelatinovorus (Ruegeria gelatinovora)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Thalassobius

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3764 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0P1FF59|A0A0P1FF59_THAGE Nicotinate dehydrogenase FAD-subunit OS=Thalassobius gelatinovorus OX=53501 GN=ndhF PE=4 SV=1
MM1 pKa = 7.45NEE3 pKa = 3.72NAEE6 pKa = 4.21PLEE9 pKa = 4.24GEE11 pKa = 4.25PLIAPSSIDD20 pKa = 3.26HH21 pKa = 6.87PLYY24 pKa = 9.35EE25 pKa = 4.38QVVEE29 pKa = 4.35ACRR32 pKa = 11.84TVYY35 pKa = 10.74DD36 pKa = 3.74PEE38 pKa = 5.17IPVNIYY44 pKa = 10.8DD45 pKa = 4.28LGLIYY50 pKa = 9.57TVKK53 pKa = 9.19ITPEE57 pKa = 3.99NKK59 pKa = 9.52VSIIMTLTAPGCPVAGDD76 pKa = 3.56MPGWVVDD83 pKa = 3.92AVEE86 pKa = 4.51PLDD89 pKa = 4.22GVQHH93 pKa = 6.03VEE95 pKa = 5.36VDD97 pKa = 4.2LTWDD101 pKa = 4.09PPWGMEE107 pKa = 3.9MMSDD111 pKa = 3.74EE112 pKa = 4.65ARR114 pKa = 11.84LEE116 pKa = 4.14LGFMM120 pKa = 4.54

Molecular weight: 13.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0P1G141|A0A0P1G141_THAGE Alanine racemase OS=Thalassobius gelatinovorus OX=53501 GN=TG4357_01871 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.31QPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.55ILNSRR34 pKa = 11.84RR35 pKa = 11.84AHH37 pKa = 5.31GRR39 pKa = 11.84KK40 pKa = 9.31SLSAA44 pKa = 3.91

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3764

0

3764

1164479

31

2130

309.4

33.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.542 ± 0.052

0.891 ± 0.011

6.41 ± 0.039

5.676 ± 0.036

3.754 ± 0.026

8.549 ± 0.039

2.057 ± 0.017

5.445 ± 0.028

3.567 ± 0.034

10.051 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.9 ± 0.018

2.843 ± 0.021

4.898 ± 0.03

3.451 ± 0.024

6.297 ± 0.031

5.336 ± 0.029

5.511 ± 0.026

7.213 ± 0.031

1.344 ± 0.015

2.266 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski