Giant panda circovirus 2
Average proteome isoelectric point is 6.86
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A220IGS9|A0A220IGS9_9CIRC Capsid protein OS=Giant panda circovirus 2 OX=2016457 PE=4 SV=1
MM1 pKa = 8.15 DD2 pKa = 4.36 ICLHH6 pKa = 6.28 CKK8 pKa = 10.19 RR9 pKa = 11.84 SPLHH13 pKa = 5.85 WRR15 pKa = 11.84 WTICSMVEE23 pKa = 3.87 NQLPQQDD30 pKa = 3.67 SDD32 pKa = 3.21 ICRR35 pKa = 11.84 AILSSLRR42 pKa = 11.84 DD43 pKa = 3.56 ADD45 pKa = 3.61 STLCEE50 pKa = 4.03 RR51 pKa = 11.84 CFLEE55 pKa = 3.76 HH56 pKa = 6.23 TLNVRR61 pKa = 11.84 KK62 pKa = 9.83 ALTNKK67 pKa = 9.04 TMTIAQRR74 pKa = 11.84 TEE76 pKa = 3.82 TLKK79 pKa = 11.14 NLVNSPPTKK88 pKa = 9.38 EE89 pKa = 3.63 ASVPVFGNLTEE100 pKa = 4.85 NGASPWTTSPLIWKK114 pKa = 9.2 SLNPFPLCTEE124 pKa = 5.38 DD125 pKa = 2.98 IAVRR129 pKa = 11.84 ADD131 pKa = 3.8 LSQTFTALAQQSEE144 pKa = 4.91 SEE146 pKa = 4.21 SSEE149 pKa = 4.02 TGSQSSSSASKK160 pKa = 9.78 MSPTIEE166 pKa = 4.04 QLNSSSTSLEE176 pKa = 3.81 EE177 pKa = 4.31 SVSLGSADD185 pKa = 3.39 TYY187 pKa = 11.26 SANN190 pKa = 3.6
Molecular weight: 20.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.983
IPC2_protein 5.08
IPC_protein 4.952
Toseland 4.863
ProMoST 5.067
Dawson 4.914
Bjellqvist 5.054
Wikipedia 4.774
Rodwell 4.838
Grimsley 4.787
Solomon 4.914
Lehninger 4.876
Nozaki 5.041
DTASelect 5.156
Thurlkill 4.863
EMBOSS 4.813
Sillero 5.105
Patrickios 3.961
IPC_peptide 4.927
IPC2_peptide 5.105
IPC2.peptide.svr19 5.08
Protein with the highest isoelectric point:
>tr|A0A220IGT9|A0A220IGT9_9CIRC Replication-associated protein OS=Giant panda circovirus 2 OX=2016457 PE=4 SV=1
MM1 pKa = 7.5 AALVPYY7 pKa = 10.14 VGRR10 pKa = 11.84 LAMRR14 pKa = 11.84 AGLSNPYY21 pKa = 10.2 ARR23 pKa = 11.84 RR24 pKa = 11.84 AGYY27 pKa = 10.8 AFAGRR32 pKa = 11.84 VAGMAARR39 pKa = 11.84 AGMKK43 pKa = 9.57 RR44 pKa = 11.84 GYY46 pKa = 9.59 RR47 pKa = 11.84 AVRR50 pKa = 11.84 RR51 pKa = 11.84 FARR54 pKa = 11.84 RR55 pKa = 11.84 PRR57 pKa = 11.84 AKK59 pKa = 10.02 RR60 pKa = 11.84 QRR62 pKa = 11.84 LNSRR66 pKa = 11.84 SHH68 pKa = 6.88 VGEE71 pKa = 4.78 AIGTTNCKK79 pKa = 8.94 TKK81 pKa = 10.38 QCSLTDD87 pKa = 3.26 ITAKK91 pKa = 10.35 NSRR94 pKa = 11.84 TLYY97 pKa = 8.95 RR98 pKa = 11.84 TGVTDD103 pKa = 4.23 IPGGSNPDD111 pKa = 2.91 EE112 pKa = 4.04 RR113 pKa = 11.84 LRR115 pKa = 11.84 NHH117 pKa = 5.79 VNLRR121 pKa = 11.84 GFKK124 pKa = 10.13 VCLEE128 pKa = 3.81 FRR130 pKa = 11.84 NNVTTPLYY138 pKa = 11.12 VNVAVLAPKK147 pKa = 8.73 NTSGNILSEE156 pKa = 3.97 TDD158 pKa = 3.71 FFRR161 pKa = 11.84 DD162 pKa = 3.37 SGTTRR167 pKa = 11.84 AKK169 pKa = 10.76 DD170 pKa = 3.35 FSTALTAMEE179 pKa = 4.42 FHH181 pKa = 7.1 CLPVNTDD188 pKa = 3.48 DD189 pKa = 4.92 YY190 pKa = 12.07 VVLKK194 pKa = 10.28 HH195 pKa = 6.2 KK196 pKa = 10.51 RR197 pKa = 11.84 FRR199 pKa = 11.84 LGPLPGATLYY209 pKa = 10.88 QNSVGNNYY217 pKa = 10.23 KK218 pKa = 10.59 NVDD221 pKa = 3.12 WYY223 pKa = 10.42 IKK225 pKa = 10.13 LGRR228 pKa = 11.84 QVRR231 pKa = 11.84 YY232 pKa = 9.44 EE233 pKa = 4.14 SSSSQATDD241 pKa = 2.89 GHH243 pKa = 6.74 VSVAYY248 pKa = 9.15 WCDD251 pKa = 2.78 AWGAAAGTAAASGSMSVSQRR271 pKa = 11.84 YY272 pKa = 4.83 ITYY275 pKa = 8.96 WKK277 pKa = 10.05 EE278 pKa = 3.66 PKK280 pKa = 9.02 TT281 pKa = 3.79
Molecular weight: 31.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.323
IPC2_protein 9.794
IPC_protein 10.496
Toseland 10.379
ProMoST 10.145
Dawson 10.57
Bjellqvist 10.306
Wikipedia 10.789
Rodwell 10.804
Grimsley 10.643
Solomon 10.628
Lehninger 10.584
Nozaki 10.379
DTASelect 10.292
Thurlkill 10.423
EMBOSS 10.789
Sillero 10.496
Patrickios 10.394
IPC_peptide 10.613
IPC2_peptide 9.341
IPC2.peptide.svr19 8.481
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
792
190
321
264.0
29.53
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.081 ± 1.555
2.904 ± 0.508
5.177 ± 0.845
5.177 ± 1.22
3.409 ± 0.473
6.313 ± 1.201
2.273 ± 0.41
3.157 ± 0.466
5.051 ± 0.45
9.217 ± 1.126
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.399 ± 0.199
4.293 ± 1.092
4.672 ± 0.341
3.03 ± 0.51
7.576 ± 1.29
9.343 ± 1.921
6.439 ± 1.604
6.061 ± 0.748
1.641 ± 0.134
3.788 ± 0.905
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here