Deer faeces associated circular DNA virus 1
Average proteome isoelectric point is 7.93
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A161IBA2|A0A161IBA2_9VIRU Replication associated protein OS=Deer faeces associated circular DNA virus 1 OX=1843767 PE=4 SV=1
MM1 pKa = 8.24 RR2 pKa = 11.84 SRR4 pKa = 11.84 TYY6 pKa = 10.43 CYY8 pKa = 10.02 TSFKK12 pKa = 10.31 EE13 pKa = 4.03 SEE15 pKa = 4.25 PFVDD19 pKa = 3.07 INQIVFQIHH28 pKa = 4.72 QLEE31 pKa = 4.3 ICEE34 pKa = 4.52 TTGRR38 pKa = 11.84 PHH40 pKa = 5.86 WQGIVAFPNAKK51 pKa = 10.25 APVGVKK57 pKa = 9.91 QYY59 pKa = 11.37 LCDD62 pKa = 3.49 TSAHH66 pKa = 6.17 IEE68 pKa = 4.01 YY69 pKa = 9.01 TKK71 pKa = 10.72 NIPASIAYY79 pKa = 7.01 CTKK82 pKa = 10.34 EE83 pKa = 3.86 EE84 pKa = 4.16 TRR86 pKa = 11.84 VDD88 pKa = 3.86 GPFSYY93 pKa = 9.09 GTVPDD98 pKa = 4.94 LEE100 pKa = 4.99 PDD102 pKa = 3.33 NWWQQLSDD110 pKa = 3.55 VQLWTEE116 pKa = 3.67 HH117 pKa = 6.04 SKK119 pKa = 10.48 WMLRR123 pKa = 11.84 NYY125 pKa = 10.55 NGVAAFRR132 pKa = 11.84 KK133 pKa = 9.84 SIKK136 pKa = 9.97 CVYY139 pKa = 10.06 APRR142 pKa = 11.84 ATPTVILLFGEE153 pKa = 4.41 TGSGKK158 pKa = 10.11 SRR160 pKa = 11.84 AARR163 pKa = 11.84 EE164 pKa = 3.87 LAGDD168 pKa = 3.77 EE169 pKa = 5.17 LYY171 pKa = 10.01 PKK173 pKa = 10.58 PMGAWWDD180 pKa = 3.9 GYY182 pKa = 9.91 RR183 pKa = 11.84 GHH185 pKa = 6.94 RR186 pKa = 11.84 VVLFDD191 pKa = 4.73 DD192 pKa = 5.41 FYY194 pKa = 11.9 GNEE197 pKa = 4.06 QYY199 pKa = 11.44 SDD201 pKa = 3.68 LLRR204 pKa = 11.84 WLSEE208 pKa = 4.04 LPISVPYY215 pKa = 10.38 KK216 pKa = 10.84 GGFTPLLADD225 pKa = 3.78 RR226 pKa = 11.84 FFFTSNAHH234 pKa = 5.82 PRR236 pKa = 11.84 DD237 pKa = 4.05 WYY239 pKa = 10.56 PNIPDD244 pKa = 4.71 KK245 pKa = 11.05 SALWRR250 pKa = 11.84 RR251 pKa = 11.84 VTKK254 pKa = 10.16 IYY256 pKa = 10.94 EE257 pKa = 4.16 CFLDD261 pKa = 5.14 RR262 pKa = 11.84 FLICDD267 pKa = 3.9 KK268 pKa = 10.91 KK269 pKa = 10.59 STLII273 pKa = 3.96
Molecular weight: 31.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.687
IPC2_protein 6.81
IPC_protein 6.898
Toseland 6.707
ProMoST 7.366
Dawson 7.512
Bjellqvist 7.585
Wikipedia 7.439
Rodwell 7.497
Grimsley 6.766
Solomon 7.571
Lehninger 7.585
Nozaki 7.863
DTASelect 7.673
Thurlkill 7.717
EMBOSS 7.775
Sillero 7.966
Patrickios 3.923
IPC_peptide 7.571
IPC2_peptide 7.22
IPC2.peptide.svr19 7.159
Protein with the highest isoelectric point:
>tr|A0A161IBA2|A0A161IBA2_9VIRU Replication associated protein OS=Deer faeces associated circular DNA virus 1 OX=1843767 PE=4 SV=1
MM1 pKa = 7.36 SLGVIPRR8 pKa = 11.84 VWPLIARR15 pKa = 11.84 GIQRR19 pKa = 11.84 LEE21 pKa = 4.17 TYY23 pKa = 10.07 PGGPRR28 pKa = 11.84 GFAEE32 pKa = 3.9 DD33 pKa = 3.48 VVRR36 pKa = 11.84 GVGYY40 pKa = 10.09 GIQGYY45 pKa = 9.03 KK46 pKa = 8.12 VAKK49 pKa = 10.02 NIAKK53 pKa = 10.09 AYY55 pKa = 8.99 GVHH58 pKa = 6.28 KK59 pKa = 10.49 VIEE62 pKa = 4.31 AEE64 pKa = 4.01 RR65 pKa = 11.84 SVKK68 pKa = 10.16 KK69 pKa = 10.24 SRR71 pKa = 11.84 KK72 pKa = 9.17 NIQSIDD78 pKa = 3.43 NPFMVKK84 pKa = 9.57 RR85 pKa = 11.84 RR86 pKa = 11.84 FAGSIIRR93 pKa = 11.84 RR94 pKa = 11.84 RR95 pKa = 11.84 RR96 pKa = 11.84 RR97 pKa = 11.84 RR98 pKa = 11.84 PLRR101 pKa = 11.84 RR102 pKa = 11.84 PTHH105 pKa = 6.51 RR106 pKa = 11.84 FRR108 pKa = 11.84 AGKK111 pKa = 10.04 SRR113 pKa = 11.84 FGHH116 pKa = 4.71 GRR118 pKa = 11.84 RR119 pKa = 11.84 RR120 pKa = 11.84 FGRR123 pKa = 11.84 RR124 pKa = 11.84 FGRR127 pKa = 11.84 GGVKK131 pKa = 9.45 RR132 pKa = 11.84 VHH134 pKa = 6.14 HH135 pKa = 7.31 AIPTVTSRR143 pKa = 11.84 EE144 pKa = 3.89 FSMVTSFADD153 pKa = 3.92 GARR156 pKa = 11.84 YY157 pKa = 9.32 LPLSIANSVTPSQFFQFFKK176 pKa = 10.35 EE177 pKa = 4.15 YY178 pKa = 10.22 RR179 pKa = 11.84 LEE181 pKa = 4.31 RR182 pKa = 11.84 CLVALRR188 pKa = 11.84 RR189 pKa = 11.84 RR190 pKa = 11.84 RR191 pKa = 11.84 PPCSVFEE198 pKa = 4.32 SATGGNTVAAGRR210 pKa = 11.84 EE211 pKa = 4.11 EE212 pKa = 4.25 TNDD215 pKa = 3.46 IYY217 pKa = 10.47 WVPWSTTDD225 pKa = 5.4 LPVQHH230 pKa = 6.86 PRR232 pKa = 11.84 QIKK235 pKa = 7.45 SARR238 pKa = 11.84 MLTAMWSVCPIRR250 pKa = 11.84 QRR252 pKa = 11.84 CIEE255 pKa = 4.12 MKK257 pKa = 10.54 LINRR261 pKa = 11.84 PLDD264 pKa = 3.85 DD265 pKa = 4.37 FLATIGGVPPNQIVTDD281 pKa = 4.01 NSWAGTTQKK290 pKa = 10.43 FPGYY294 pKa = 10.15 AVQSWTRR301 pKa = 11.84 MPWQQTDD308 pKa = 2.77 ASLGDD313 pKa = 3.37 RR314 pKa = 11.84 HH315 pKa = 6.24 LVHH318 pKa = 7.02 VSQLLVLFLCLVLRR332 pKa = 11.84 LFLVFRR338 pKa = 11.84 ILIPFRR344 pKa = 11.84 LTVFGG349 pKa = 4.4
Molecular weight: 39.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.173
IPC2_protein 10.116
IPC_protein 11.418
Toseland 11.55
ProMoST 12.003
Dawson 11.564
Bjellqvist 11.535
Wikipedia 12.018
Rodwell 11.359
Grimsley 11.608
Solomon 12.018
Lehninger 11.93
Nozaki 11.55
DTASelect 11.535
Thurlkill 11.55
EMBOSS 12.047
Sillero 11.55
Patrickios 11.067
IPC_peptide 12.032
IPC2_peptide 11.008
IPC2.peptide.svr19 9.37
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
622
273
349
311.0
35.8
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.27 ± 0.025
1.929 ± 0.372
4.18 ± 0.986
4.18 ± 0.771
5.788 ± 0.172
7.074 ± 0.712
2.251 ± 0.031
5.788 ± 0.172
4.662 ± 0.488
7.556 ± 0.08
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.608 ± 0.299
2.894 ± 0.236
6.431 ± 0.095
3.698 ± 0.235
10.129 ± 2.289
6.109 ± 0.069
5.788 ± 0.258
7.235 ± 1.021
2.733 ± 0.546
3.698 ± 1.054
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here