Amino acid dipepetide frequency for Streptomyces phage Satis

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.097AlaAla: 12.097 ± 1.211
0.907AlaCys: 0.907 ± 0.131
6.729AlaAsp: 6.729 ± 0.375
7.871AlaGlu: 7.871 ± 0.504
2.938AlaPhe: 2.938 ± 0.243
7.835AlaGly: 7.835 ± 0.582
1.832AlaHis: 1.832 ± 0.173
4.008AlaIle: 4.008 ± 0.429
3.918AlaLys: 3.918 ± 0.332
9.286AlaLeu: 9.286 ± 0.381
2.848AlaMet: 2.848 ± 0.245
2.684AlaAsn: 2.684 ± 0.286
4.77AlaPro: 4.77 ± 0.321
3.972AlaGln: 3.972 ± 0.486
6.856AlaArg: 6.856 ± 0.335
5.786AlaSer: 5.786 ± 0.365
5.659AlaThr: 5.659 ± 0.618
7.273AlaVal: 7.273 ± 0.395
1.687AlaTrp: 1.687 ± 0.17
2.866AlaTyr: 2.866 ± 0.233
0.0AlaXaa: 0.0 ± 0.0
Cys
0.707CysAla: 0.707 ± 0.123
0.236CysCys: 0.236 ± 0.064
0.707CysAsp: 0.707 ± 0.125
0.798CysGlu: 0.798 ± 0.127
0.254CysPhe: 0.254 ± 0.071
1.505CysGly: 1.505 ± 0.191
0.218CysHis: 0.218 ± 0.067
0.272CysIle: 0.272 ± 0.075
0.49CysLys: 0.49 ± 0.092
0.943CysLeu: 0.943 ± 0.146
0.145CysMet: 0.145 ± 0.05
0.181CysAsn: 0.181 ± 0.063
0.961CysPro: 0.961 ± 0.17
0.272CysGln: 0.272 ± 0.064
1.052CysArg: 1.052 ± 0.142
0.617CysSer: 0.617 ± 0.105
0.617CysThr: 0.617 ± 0.108
0.58CysVal: 0.58 ± 0.114
0.218CysTrp: 0.218 ± 0.056
0.272CysTyr: 0.272 ± 0.072
0.0CysXaa: 0.0 ± 0.0
Asp
5.895AspAla: 5.895 ± 0.386
0.725AspCys: 0.725 ± 0.115
4.19AspAsp: 4.19 ± 0.274
5.096AspGlu: 5.096 ± 0.295
1.977AspPhe: 1.977 ± 0.231
6.783AspGly: 6.783 ± 0.395
1.614AspHis: 1.614 ± 0.191
2.594AspIle: 2.594 ± 0.208
1.959AspLys: 1.959 ± 0.212
5.459AspLeu: 5.459 ± 0.355
1.632AspMet: 1.632 ± 0.174
1.433AspAsn: 1.433 ± 0.176
4.099AspPro: 4.099 ± 0.26
2.086AspGln: 2.086 ± 0.165
4.625AspArg: 4.625 ± 0.326
2.993AspSer: 2.993 ± 0.3
2.956AspThr: 2.956 ± 0.247
4.643AspVal: 4.643 ± 0.276
1.705AspTrp: 1.705 ± 0.168
1.415AspTyr: 1.415 ± 0.163
0.0AspXaa: 0.0 ± 0.0
Glu
7.055GluAla: 7.055 ± 0.43
0.617GluCys: 0.617 ± 0.13
4.208GluAsp: 4.208 ± 0.314
5.876GluGlu: 5.876 ± 0.463
2.122GluPhe: 2.122 ± 0.191
5.731GluGly: 5.731 ± 0.33
1.868GluHis: 1.868 ± 0.173
3.428GluIle: 3.428 ± 0.275
3.519GluLys: 3.519 ± 0.283
5.931GluLeu: 5.931 ± 0.4
2.249GluMet: 2.249 ± 0.201
2.285GluAsn: 2.285 ± 0.202
3.21GluPro: 3.21 ± 0.316
2.575GluGln: 2.575 ± 0.245
5.369GluArg: 5.369 ± 0.42
3.646GluSer: 3.646 ± 0.26
4.172GluThr: 4.172 ± 0.267
4.679GluVal: 4.679 ± 0.268
1.378GluTrp: 1.378 ± 0.161
2.122GluTyr: 2.122 ± 0.218
0.0GluXaa: 0.0 ± 0.0
Phe
2.358PheAla: 2.358 ± 0.228
0.725PheCys: 0.725 ± 0.112
2.068PheAsp: 2.068 ± 0.18
2.195PheGlu: 2.195 ± 0.184
0.943PhePhe: 0.943 ± 0.146
2.412PheGly: 2.412 ± 0.212
0.635PheHis: 0.635 ± 0.13
1.052PheIle: 1.052 ± 0.181
0.943PheLys: 0.943 ± 0.138
2.503PheLeu: 2.503 ± 0.22
0.58PheMet: 0.58 ± 0.103
0.78PheAsn: 0.78 ± 0.119
1.723PhePro: 1.723 ± 0.173
1.034PheGln: 1.034 ± 0.169
1.777PheArg: 1.777 ± 0.188
1.886PheSer: 1.886 ± 0.218
2.068PheThr: 2.068 ± 0.208
1.759PheVal: 1.759 ± 0.176
0.562PheTrp: 0.562 ± 0.093
0.689PheTyr: 0.689 ± 0.122
0.0PheXaa: 0.0 ± 0.0
Gly
7.545GlyAla: 7.545 ± 0.653
0.998GlyCys: 0.998 ± 0.132
5.677GlyAsp: 5.677 ± 0.354
6.058GlyGlu: 6.058 ± 0.36
3.029GlyPhe: 3.029 ± 0.224
6.965GlyGly: 6.965 ± 0.516
2.068GlyHis: 2.068 ± 0.16
3.936GlyIle: 3.936 ± 0.287
3.899GlyLys: 3.899 ± 0.311
6.765GlyLeu: 6.765 ± 0.336
2.376GlyMet: 2.376 ± 0.184
2.448GlyAsn: 2.448 ± 0.303
4.679GlyPro: 4.679 ± 0.398
2.92GlyGln: 2.92 ± 0.196
5.731GlyArg: 5.731 ± 0.365
6.547GlySer: 6.547 ± 0.427
6.221GlyThr: 6.221 ± 0.469
6.058GlyVal: 6.058 ± 0.354
2.195GlyTrp: 2.195 ± 0.241
2.213GlyTyr: 2.213 ± 0.201
0.0GlyXaa: 0.0 ± 0.0
His
2.031HisAla: 2.031 ± 0.195
0.236HisCys: 0.236 ± 0.074
1.415HisAsp: 1.415 ± 0.178
1.632HisGlu: 1.632 ± 0.216
0.599HisPhe: 0.599 ± 0.102
2.195HisGly: 2.195 ± 0.219
0.472HisHis: 0.472 ± 0.107
0.798HisIle: 0.798 ± 0.124
0.635HisLys: 0.635 ± 0.12
2.049HisLeu: 2.049 ± 0.214
0.381HisMet: 0.381 ± 0.084
0.49HisAsn: 0.49 ± 0.099
1.614HisPro: 1.614 ± 0.175
0.725HisGln: 0.725 ± 0.108
1.487HisArg: 1.487 ± 0.204
0.907HisSer: 0.907 ± 0.131
1.342HisThr: 1.342 ± 0.161
1.56HisVal: 1.56 ± 0.171
0.363HisTrp: 0.363 ± 0.086
0.744HisTyr: 0.744 ± 0.113
0.0HisXaa: 0.0 ± 0.0
Ile
3.899IleAla: 3.899 ± 0.272
0.544IleCys: 0.544 ± 0.109
3.047IleAsp: 3.047 ± 0.27
2.993IleGlu: 2.993 ± 0.279
0.943IlePhe: 0.943 ± 0.137
3.265IleGly: 3.265 ± 0.23
0.852IleHis: 0.852 ± 0.122
0.979IleIle: 0.979 ± 0.135
1.524IleLys: 1.524 ± 0.18
3.337IleLeu: 3.337 ± 0.21
0.889IleMet: 0.889 ± 0.115
1.215IleAsn: 1.215 ± 0.174
2.757IlePro: 2.757 ± 0.228
1.215IleGln: 1.215 ± 0.148
3.373IleArg: 3.373 ± 0.241
2.848IleSer: 2.848 ± 0.245
3.138IleThr: 3.138 ± 0.219
2.684IleVal: 2.684 ± 0.256
0.58IleTrp: 0.58 ± 0.092
0.816IleTyr: 0.816 ± 0.114
0.0IleXaa: 0.0 ± 0.0
Lys
4.516LysAla: 4.516 ± 0.324
0.345LysCys: 0.345 ± 0.07
2.267LysAsp: 2.267 ± 0.215
2.358LysGlu: 2.358 ± 0.197
1.07LysPhe: 1.07 ± 0.13
3.718LysGly: 3.718 ± 0.352
0.889LysHis: 0.889 ± 0.148
2.068LysIle: 2.068 ± 0.184
2.358LysLys: 2.358 ± 0.25
3.845LysLeu: 3.845 ± 0.346
1.088LysMet: 1.088 ± 0.148
1.36LysAsn: 1.36 ± 0.156
2.122LysPro: 2.122 ± 0.191
1.36LysGln: 1.36 ± 0.149
2.521LysArg: 2.521 ± 0.23
2.013LysSer: 2.013 ± 0.265
2.503LysThr: 2.503 ± 0.249
3.011LysVal: 3.011 ± 0.274
0.834LysTrp: 0.834 ± 0.12
0.925LysTyr: 0.925 ± 0.15
0.0LysXaa: 0.0 ± 0.0
Leu
9.993LeuAla: 9.993 ± 0.647
0.889LeuCys: 0.889 ± 0.147
5.441LeuAsp: 5.441 ± 0.334
4.97LeuGlu: 4.97 ± 0.312
1.85LeuPhe: 1.85 ± 0.162
6.42LeuGly: 6.42 ± 0.315
1.741LeuHis: 1.741 ± 0.186
3.355LeuIle: 3.355 ± 0.214
4.008LeuLys: 4.008 ± 0.252
6.928LeuLeu: 6.928 ± 0.465
2.213LeuMet: 2.213 ± 0.234
2.594LeuAsn: 2.594 ± 0.216
4.534LeuPro: 4.534 ± 0.325
1.705LeuGln: 1.705 ± 0.169
5.731LeuArg: 5.731 ± 0.381
4.48LeuSer: 4.48 ± 0.318
5.604LeuThr: 5.604 ± 0.258
5.786LeuVal: 5.786 ± 0.34
1.143LeuTrp: 1.143 ± 0.189
2.213LeuTyr: 2.213 ± 0.242
0.0LeuXaa: 0.0 ± 0.0
Met
3.682MetAla: 3.682 ± 0.27
0.127MetCys: 0.127 ± 0.051
1.868MetAsp: 1.868 ± 0.164
1.524MetGlu: 1.524 ± 0.158
0.544MetPhe: 0.544 ± 0.103
1.832MetGly: 1.832 ± 0.156
0.544MetHis: 0.544 ± 0.109
1.016MetIle: 1.016 ± 0.136
1.487MetLys: 1.487 ± 0.191
1.759MetLeu: 1.759 ± 0.209
0.562MetMet: 0.562 ± 0.123
1.251MetAsn: 1.251 ± 0.173
1.179MetPro: 1.179 ± 0.153
0.998MetGln: 0.998 ± 0.113
1.904MetArg: 1.904 ± 0.21
1.85MetSer: 1.85 ± 0.179
1.923MetThr: 1.923 ± 0.161
1.487MetVal: 1.487 ± 0.141
0.508MetTrp: 0.508 ± 0.095
0.508MetTyr: 0.508 ± 0.11
0.0MetXaa: 0.0 ± 0.0
Asn
3.174AsnAla: 3.174 ± 0.295
0.236AsnCys: 0.236 ± 0.061
1.56AsnAsp: 1.56 ± 0.16
1.814AsnGlu: 1.814 ± 0.172
0.635AsnPhe: 0.635 ± 0.131
3.718AsnGly: 3.718 ± 0.309
0.58AsnHis: 0.58 ± 0.095
0.979AsnIle: 0.979 ± 0.13
1.016AsnLys: 1.016 ± 0.147
2.376AsnLeu: 2.376 ± 0.269
0.617AsnMet: 0.617 ± 0.114
0.871AsnAsn: 0.871 ± 0.153
2.249AsnPro: 2.249 ± 0.218
0.925AsnGln: 0.925 ± 0.149
1.832AsnArg: 1.832 ± 0.207
1.542AsnSer: 1.542 ± 0.216
1.542AsnThr: 1.542 ± 0.203
1.904AsnVal: 1.904 ± 0.18
0.381AsnTrp: 0.381 ± 0.086
0.725AsnTyr: 0.725 ± 0.13
0.0AsnXaa: 0.0 ± 0.0
Pro
4.861ProAla: 4.861 ± 0.313
0.816ProCys: 0.816 ± 0.154
3.646ProAsp: 3.646 ± 0.283
5.441ProGlu: 5.441 ± 0.345
1.36ProPhe: 1.36 ± 0.171
6.021ProGly: 6.021 ± 0.393
1.179ProHis: 1.179 ± 0.146
1.36ProIle: 1.36 ± 0.154
2.104ProLys: 2.104 ± 0.213
3.555ProLeu: 3.555 ± 0.297
1.397ProMet: 1.397 ± 0.167
1.251ProAsn: 1.251 ± 0.15
3.047ProPro: 3.047 ± 0.28
2.049ProGln: 2.049 ± 0.226
3.156ProArg: 3.156 ± 0.273
3.065ProSer: 3.065 ± 0.279
2.829ProThr: 2.829 ± 0.241
4.879ProVal: 4.879 ± 0.32
0.943ProTrp: 0.943 ± 0.153
1.36ProTyr: 1.36 ± 0.16
0.0ProXaa: 0.0 ± 0.0
Gln
3.664GlnAla: 3.664 ± 0.372
0.417GlnCys: 0.417 ± 0.084
1.796GlnAsp: 1.796 ± 0.181
2.086GlnGlu: 2.086 ± 0.211
0.998GlnPhe: 0.998 ± 0.147
2.829GlnGly: 2.829 ± 0.21
0.852GlnHis: 0.852 ± 0.125
1.959GlnIle: 1.959 ± 0.206
1.433GlnLys: 1.433 ± 0.22
2.104GlnLeu: 2.104 ± 0.235
0.816GlnMet: 0.816 ± 0.122
1.016GlnAsn: 1.016 ± 0.141
1.433GlnPro: 1.433 ± 0.154
1.36GlnGln: 1.36 ± 0.199
2.322GlnArg: 2.322 ± 0.235
1.505GlnSer: 1.505 ± 0.163
1.451GlnThr: 1.451 ± 0.158
2.503GlnVal: 2.503 ± 0.244
0.599GlnTrp: 0.599 ± 0.1
1.088GlnTyr: 1.088 ± 0.129
0.0GlnXaa: 0.0 ± 0.0
Arg
6.674ArgAla: 6.674 ± 0.386
0.689ArgCys: 0.689 ± 0.106
4.552ArgAsp: 4.552 ± 0.299
5.804ArgGlu: 5.804 ± 0.4
2.195ArgPhe: 2.195 ± 0.184
4.806ArgGly: 4.806 ± 0.395
1.65ArgHis: 1.65 ± 0.191
3.827ArgIle: 3.827 ± 0.271
3.337ArgLys: 3.337 ± 0.355
5.677ArgLeu: 5.677 ± 0.374
2.213ArgMet: 2.213 ± 0.226
2.358ArgAsn: 2.358 ± 0.204
3.083ArgPro: 3.083 ± 0.286
2.086ArgGln: 2.086 ± 0.191
5.514ArgArg: 5.514 ± 0.414
3.537ArgSer: 3.537 ± 0.258
3.863ArgThr: 3.863 ± 0.273
4.843ArgVal: 4.843 ± 0.36
1.451ArgTrp: 1.451 ± 0.168
1.632ArgTyr: 1.632 ± 0.187
0.0ArgXaa: 0.0 ± 0.0
Ser
5.967SerAla: 5.967 ± 0.488
0.617SerCys: 0.617 ± 0.104
3.627SerAsp: 3.627 ± 0.261
3.591SerGlu: 3.591 ± 0.245
1.832SerPhe: 1.832 ± 0.185
6.62SerGly: 6.62 ± 0.468
0.889SerHis: 0.889 ± 0.122
2.14SerIle: 2.14 ± 0.19
2.231SerLys: 2.231 ± 0.225
4.425SerLeu: 4.425 ± 0.339
1.868SerMet: 1.868 ± 0.19
1.233SerAsn: 1.233 ± 0.181
2.721SerPro: 2.721 ± 0.213
1.433SerGln: 1.433 ± 0.124
4.045SerArg: 4.045 ± 0.291
3.446SerSer: 3.446 ± 0.344
3.12SerThr: 3.12 ± 0.267
4.97SerVal: 4.97 ± 0.309
1.143SerTrp: 1.143 ± 0.13
1.324SerTyr: 1.324 ± 0.182
0.0SerXaa: 0.0 ± 0.0
Thr
6.584ThrAla: 6.584 ± 0.536
0.689ThrCys: 0.689 ± 0.129
3.627ThrAsp: 3.627 ± 0.297
3.827ThrGlu: 3.827 ± 0.302
1.705ThrPhe: 1.705 ± 0.191
6.493ThrGly: 6.493 ± 0.394
1.07ThrHis: 1.07 ± 0.138
2.303ThrIle: 2.303 ± 0.198
1.977ThrLys: 1.977 ± 0.193
4.534ThrLeu: 4.534 ± 0.318
1.487ThrMet: 1.487 ± 0.169
1.705ThrAsn: 1.705 ± 0.204
3.736ThrPro: 3.736 ± 0.294
1.868ThrGln: 1.868 ± 0.205
3.555ThrArg: 3.555 ± 0.224
3.464ThrSer: 3.464 ± 0.275
3.5ThrThr: 3.5 ± 0.271
5.967ThrVal: 5.967 ± 0.396
1.27ThrTrp: 1.27 ± 0.157
1.542ThrTyr: 1.542 ± 0.183
0.0ThrXaa: 0.0 ± 0.0
Val
7.001ValAla: 7.001 ± 0.362
0.744ValCys: 0.744 ± 0.136
4.444ValAsp: 4.444 ± 0.303
5.26ValGlu: 5.26 ± 0.363
2.63ValPhe: 2.63 ± 0.205
5.169ValGly: 5.169 ± 0.406
1.814ValHis: 1.814 ± 0.202
3.265ValIle: 3.265 ± 0.25
2.739ValLys: 2.739 ± 0.265
5.695ValLeu: 5.695 ± 0.405
2.158ValMet: 2.158 ± 0.191
2.195ValAsn: 2.195 ± 0.207
4.045ValPro: 4.045 ± 0.264
2.467ValGln: 2.467 ± 0.233
5.532ValArg: 5.532 ± 0.326
4.516ValSer: 4.516 ± 0.308
5.314ValThr: 5.314 ± 0.274
5.731ValVal: 5.731 ± 0.385
1.378ValTrp: 1.378 ± 0.18
2.049ValTyr: 2.049 ± 0.185
0.0ValXaa: 0.0 ± 0.0
Trp
1.378TrpAla: 1.378 ± 0.189
0.29TrpCys: 0.29 ± 0.068
1.324TrpAsp: 1.324 ± 0.162
1.106TrpGlu: 1.106 ± 0.155
0.49TrpPhe: 0.49 ± 0.095
1.27TrpGly: 1.27 ± 0.159
0.363TrpHis: 0.363 ± 0.074
0.907TrpIle: 0.907 ± 0.144
0.852TrpLys: 0.852 ± 0.127
1.995TrpLeu: 1.995 ± 0.205
0.58TrpMet: 0.58 ± 0.09
0.58TrpAsn: 0.58 ± 0.113
0.943TrpPro: 0.943 ± 0.12
0.508TrpGln: 0.508 ± 0.09
1.306TrpArg: 1.306 ± 0.153
1.306TrpSer: 1.306 ± 0.174
1.596TrpThr: 1.596 ± 0.191
1.505TrpVal: 1.505 ± 0.178
0.49TrpTrp: 0.49 ± 0.109
0.562TrpTyr: 0.562 ± 0.105
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.739TyrAla: 2.739 ± 0.225
0.2TyrCys: 0.2 ± 0.053
1.687TyrAsp: 1.687 ± 0.181
1.741TyrGlu: 1.741 ± 0.228
0.58TyrPhe: 0.58 ± 0.098
2.739TyrGly: 2.739 ± 0.24
0.544TyrHis: 0.544 ± 0.097
0.653TyrIle: 0.653 ± 0.124
0.744TyrLys: 0.744 ± 0.131
2.43TyrLeu: 2.43 ± 0.191
0.435TyrMet: 0.435 ± 0.093
0.744TyrAsn: 0.744 ± 0.132
1.415TyrPro: 1.415 ± 0.186
0.671TyrGln: 0.671 ± 0.116
2.104TyrArg: 2.104 ± 0.22
1.288TyrSer: 1.288 ± 0.17
1.487TyrThr: 1.487 ± 0.163
2.34TyrVal: 2.34 ± 0.236
0.526TyrTrp: 0.526 ± 0.094
0.58TyrTyr: 0.58 ± 0.107
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 325 proteins (55137 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski