Inhella crocodyli
Average proteome isoelectric point is 7.1
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3430 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3S2URV5|A0A3S2URV5_9BURK Uncharacterized protein OS=Inhella crocodyli OX=2499851 GN=EOD73_15270 PE=4 SV=1
DDD2 pKa = 4.01 AVVTGVATGSVTEEE16 pKa = 5.08 DD17 pKa = 3.39 TVLASGTLIVSDDD30 pKa = 3.85 DDD32 pKa = 3.63 ATTVVPGSVAGTYYY46 pKa = 11.19 DDD48 pKa = 3.76 TINAAGQWTYYY59 pKa = 7.64 LRR61 pKa = 11.84 NGAANVQALTSADDD75 pKa = 3.7 HH76 pKa = 6.29 VEEE79 pKa = 4.34 FTVTT
Molecular weight: 8.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.802
IPC2_protein 3.681
IPC_protein 3.541
Toseland 3.363
ProMoST 3.884
Dawson 3.592
Bjellqvist 3.757
Wikipedia 3.605
Rodwell 3.401
Grimsley 3.287
Solomon 3.528
Lehninger 3.478
Nozaki 3.745
DTASelect 3.961
Thurlkill 3.478
EMBOSS 3.605
Sillero 3.694
Patrickios 1.863
IPC_peptide 3.516
IPC2_peptide 3.643
IPC2.peptide.svr19 3.658
Protein with the highest isoelectric point:
>tr|A0A437LHS8|A0A437LHS8_9BURK Cytochrome P450 OS=Inhella crocodyli OX=2499851 GN=EOD73_12575 PE=3 SV=1
MM1 pKa = 7.31 KK2 pKa = 9.39 RR3 pKa = 11.84 TYY5 pKa = 10.12 QGSKK9 pKa = 7.18 VRR11 pKa = 11.84 RR12 pKa = 11.84 ARR14 pKa = 11.84 THH16 pKa = 5.65 GFLVRR21 pKa = 11.84 MKK23 pKa = 10.53 SRR25 pKa = 11.84 GGRR28 pKa = 11.84 AVLAARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.87 GRR39 pKa = 11.84 KK40 pKa = 8.75 RR41 pKa = 11.84 LAVV44 pKa = 3.41
Molecular weight: 5.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.431
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.34
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.076
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.079
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3430
0
3430
1173898
37
3140
342.2
37.03
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.969 ± 0.066
0.856 ± 0.013
5.241 ± 0.028
5.106 ± 0.037
3.191 ± 0.024
8.462 ± 0.035
2.357 ± 0.025
3.192 ± 0.031
2.814 ± 0.035
11.679 ± 0.058
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.136 ± 0.018
2.264 ± 0.031
5.768 ± 0.04
4.487 ± 0.027
7.46 ± 0.047
4.857 ± 0.036
4.85 ± 0.046
7.631 ± 0.031
1.831 ± 0.024
1.85 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here