Amino acid dipepetide frequency for Bradyrhizobiaceae bacterium

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	17.278AlaAla: 17.278 ± 0.172	1.084AlaCys: 1.084 ± 0.034	7.005AlaAsp: 7.005 ± 0.08	6.582AlaGlu: 6.582 ± 0.069	4.422AlaPhe: 4.422 ± 0.053	10.018AlaGly: 10.018 ± 0.099	2.246AlaHis: 2.246 ± 0.043	6.969AlaIle: 6.969 ± 0.076	5.122AlaLys: 5.122 ± 0.08	12.737AlaLeu: 12.737 ± 0.119	3.832AlaMet: 3.832 ± 0.058	3.15AlaAsn: 3.15 ± 0.052	5.875AlaPro: 5.875 ± 0.09	4.413AlaGln: 4.413 ± 0.063	7.982AlaArg: 7.982 ± 0.091	6.982AlaSer: 6.982 ± 0.09	6.722AlaThr: 6.722 ± 0.079	8.88AlaVal: 8.88 ± 0.085	1.389AlaTrp: 1.389 ± 0.035	2.575AlaTyr: 2.575 ± 0.046	0.0AlaXaa: 0.0 ± 0.0
Cys	0.925CysAla: 0.925 ± 0.03	0.099CysCys: 0.099 ± 0.008	0.515CysAsp: 0.515 ± 0.025	0.377CysGlu: 0.377 ± 0.018	0.311CysPhe: 0.311 ± 0.016	0.888CysGly: 0.888 ± 0.032	0.21CysHis: 0.21 ± 0.013	0.43CysIle: 0.43 ± 0.019	0.21CysLys: 0.21 ± 0.012	0.676CysLeu: 0.676 ± 0.024	0.191CysMet: 0.191 ± 0.012	0.217CysAsn: 0.217 ± 0.014	0.383CysPro: 0.383 ± 0.02	0.225CysGln: 0.225 ± 0.013	0.538CysArg: 0.538 ± 0.023	0.453CysSer: 0.453 ± 0.019	0.426CysThr: 0.426 ± 0.019	0.624CysVal: 0.624 ± 0.023	0.097CysTrp: 0.097 ± 0.009	0.192CysTyr: 0.192 ± 0.013	0.0CysXaa: 0.0 ± 0.0
Asp	7.09AspAla: 7.09 ± 0.095	0.419AspCys: 0.419 ± 0.019	3.379AspAsp: 3.379 ± 0.065	3.161AspGlu: 3.161 ± 0.055	2.161AspPhe: 2.161 ± 0.041	5.095AspGly: 5.095 ± 0.069	1.337AspHis: 1.337 ± 0.036	3.377AspIle: 3.377 ± 0.054	2.241AspLys: 2.241 ± 0.044	5.634AspLeu: 5.634 ± 0.078	1.381AspMet: 1.381 ± 0.032	1.426AspAsn: 1.426 ± 0.036	3.222AspPro: 3.222 ± 0.05	1.722AspGln: 1.722 ± 0.037	4.006AspArg: 4.006 ± 0.068	2.257AspSer: 2.257 ± 0.04	2.727AspThr: 2.727 ± 0.053	4.589AspVal: 4.589 ± 0.057	0.845AspTrp: 0.845 ± 0.026	1.541AspTyr: 1.541 ± 0.033	0.0AspXaa: 0.0 ± 0.0
Glu	6.342GluAla: 6.342 ± 0.076	0.295GluCys: 0.295 ± 0.017	2.337GluAsp: 2.337 ± 0.043	2.212GluGlu: 2.212 ± 0.049	1.736GluPhe: 1.736 ± 0.034	3.466GluGly: 3.466 ± 0.051	1.036GluHis: 1.036 ± 0.027	3.313GluIle: 3.313 ± 0.053	2.17GluLys: 2.17 ± 0.052	4.94GluLeu: 4.94 ± 0.072	1.464GluMet: 1.464 ± 0.034	1.406GluAsn: 1.406 ± 0.033	2.322GluPro: 2.322 ± 0.042	2.081GluGln: 2.081 ± 0.041	4.163GluArg: 4.163 ± 0.067	2.195GluSer: 2.195 ± 0.04	3.072GluThr: 3.072 ± 0.053	3.516GluVal: 3.516 ± 0.049	0.594GluTrp: 0.594 ± 0.022	0.861GluTyr: 0.861 ± 0.024	0.0GluXaa: 0.0 ± 0.0
Phe	4.984PheAla: 4.984 ± 0.069	0.364PheCys: 0.364 ± 0.014	2.563PheAsp: 2.563 ± 0.048	1.935PheGlu: 1.935 ± 0.043	1.413PhePhe: 1.413 ± 0.039	3.913PheGly: 3.913 ± 0.054	0.678PheHis: 0.678 ± 0.028	1.789PheIle: 1.789 ± 0.04	1.309PheLys: 1.309 ± 0.036	3.146PheLeu: 3.146 ± 0.064	0.843PheMet: 0.843 ± 0.027	1.158PheAsn: 1.158 ± 0.035	1.587PhePro: 1.587 ± 0.037	1.016PheGln: 1.016 ± 0.029	2.095PheArg: 2.095 ± 0.039	2.167PheSer: 2.167 ± 0.037	2.153PheThr: 2.153 ± 0.044	2.898PheVal: 2.898 ± 0.051	0.515PheTrp: 0.515 ± 0.02	0.865PheTyr: 0.865 ± 0.024	0.0PheXaa: 0.0 ± 0.0
Gly	9.308GlyAla: 9.308 ± 0.087	0.807GlyCys: 0.807 ± 0.03	4.408GlyAsp: 4.408 ± 0.053	4.147GlyGlu: 4.147 ± 0.057	3.732GlyPhe: 3.732 ± 0.06	7.325GlyGly: 7.325 ± 0.11	1.86GlyHis: 1.86 ± 0.04	4.986GlyIle: 4.986 ± 0.064	3.665GlyLys: 3.665 ± 0.066	8.497GlyLeu: 8.497 ± 0.088	2.323GlyMet: 2.323 ± 0.041	2.315GlyAsn: 2.315 ± 0.048	3.378GlyPro: 3.378 ± 0.055	2.81GlyGln: 2.81 ± 0.049	5.414GlyArg: 5.414 ± 0.062	4.67GlySer: 4.67 ± 0.063	4.694GlyThr: 4.694 ± 0.083	6.206GlyVal: 6.206 ± 0.069	1.262GlyTrp: 1.262 ± 0.033	2.357GlyTyr: 2.357 ± 0.05	0.0GlyXaa: 0.0 ± 0.0
His	2.418HisAla: 2.418 ± 0.047	0.216HisCys: 0.216 ± 0.013	1.335HisAsp: 1.335 ± 0.032	0.897HisGlu: 0.897 ± 0.027	0.807HisPhe: 0.807 ± 0.026	1.996HisGly: 1.996 ± 0.049	0.603HisHis: 0.603 ± 0.025	0.946HisIle: 0.946 ± 0.027	0.507HisLys: 0.507 ± 0.023	1.904HisLeu: 1.904 ± 0.042	0.496HisMet: 0.496 ± 0.02	0.459HisAsn: 0.459 ± 0.019	1.3HisPro: 1.3 ± 0.038	0.617HisGln: 0.617 ± 0.018	1.393HisArg: 1.393 ± 0.034	0.91HisSer: 0.91 ± 0.025	0.82HisThr: 0.82 ± 0.025	1.538HisVal: 1.538 ± 0.032	0.3HisTrp: 0.3 ± 0.015	0.54HisTyr: 0.54 ± 0.02	0.0HisXaa: 0.0 ± 0.0
Ile	8.236IleAla: 8.236 ± 0.088	0.551IleCys: 0.551 ± 0.024	4.106IleAsp: 4.106 ± 0.061	3.42IleGlu: 3.42 ± 0.048	1.757IlePhe: 1.757 ± 0.037	5.586IleGly: 5.586 ± 0.082	0.932IleHis: 0.932 ± 0.028	2.511IleIle: 2.511 ± 0.053	2.043IleLys: 2.043 ± 0.045	4.225IleLeu: 4.225 ± 0.063	1.13IleMet: 1.13 ± 0.031	1.634IleAsn: 1.634 ± 0.034	2.386IlePro: 2.386 ± 0.049	1.268IleGln: 1.268 ± 0.03	3.173IleArg: 3.173 ± 0.049	3.21IleSer: 3.21 ± 0.05	3.093IleThr: 3.093 ± 0.054	4.747IleVal: 4.747 ± 0.058	0.617IleTrp: 0.617 ± 0.025	1.269IleTyr: 1.269 ± 0.035	0.0IleXaa: 0.0 ± 0.0
Lys	4.744LysAla: 4.744 ± 0.085	0.176LysCys: 0.176 ± 0.013	2.078LysAsp: 2.078 ± 0.046	1.624LysGlu: 1.624 ± 0.043	1.206LysPhe: 1.206 ± 0.03	2.798LysGly: 2.798 ± 0.055	0.748LysHis: 0.748 ± 0.025	2.171LysIle: 2.171 ± 0.046	1.795LysLys: 1.795 ± 0.052	4.077LysLeu: 4.077 ± 0.058	1.041LysMet: 1.041 ± 0.03	1.081LysAsn: 1.081 ± 0.027	2.665LysPro: 2.665 ± 0.053	1.365LysGln: 1.365 ± 0.034	2.593LysArg: 2.593 ± 0.05	2.216LysSer: 2.216 ± 0.044	2.299LysThr: 2.299 ± 0.048	2.797LysVal: 2.797 ± 0.05	0.411LysTrp: 0.411 ± 0.017	0.804LysTyr: 0.804 ± 0.029	0.0LysXaa: 0.0 ± 0.0
Leu	12.804LeuAla: 12.804 ± 0.113	0.82LeuCys: 0.82 ± 0.025	5.716LeuAsp: 5.716 ± 0.072	4.21LeuGlu: 4.21 ± 0.062	3.542LeuPhe: 3.542 ± 0.059	8.085LeuGly: 8.085 ± 0.08	1.816LeuHis: 1.816 ± 0.038	5.284LeuIle: 5.284 ± 0.071	3.951LeuLys: 3.951 ± 0.067	9.414LeuLeu: 9.414 ± 0.13	2.537LeuMet: 2.537 ± 0.048	2.722LeuAsn: 2.722 ± 0.053	5.287LeuPro: 5.287 ± 0.075	3.009LeuGln: 3.009 ± 0.048	6.281LeuArg: 6.281 ± 0.085	6.055LeuSer: 6.055 ± 0.075	5.719LeuThr: 5.719 ± 0.067	7.125LeuVal: 7.125 ± 0.084	1.085LeuTrp: 1.085 ± 0.033	1.969LeuTyr: 1.969 ± 0.044	0.0LeuXaa: 0.0 ± 0.0
Met	3.242MetAla: 3.242 ± 0.049	0.176MetCys: 0.176 ± 0.012	1.122MetAsp: 1.122 ± 0.031	0.981MetGlu: 0.981 ± 0.03	0.849MetPhe: 0.849 ± 0.026	1.818MetGly: 1.818 ± 0.04	0.462MetHis: 0.462 ± 0.019	1.637MetIle: 1.637 ± 0.039	1.126MetLys: 1.126 ± 0.03	2.727MetLeu: 2.727 ± 0.049	0.814MetMet: 0.814 ± 0.028	0.82MetAsn: 0.82 ± 0.023	1.659MetPro: 1.659 ± 0.036	0.983MetGln: 0.983 ± 0.029	1.872MetArg: 1.872 ± 0.037	1.872MetSer: 1.872 ± 0.035	2.118MetThr: 2.118 ± 0.037	1.806MetVal: 1.806 ± 0.039	0.256MetTrp: 0.256 ± 0.015	0.4MetTyr: 0.4 ± 0.018	0.0MetXaa: 0.0 ± 0.0
Asn	3.472AsnAla: 3.472 ± 0.053	0.234AsnCys: 0.234 ± 0.015	1.633AsnAsp: 1.633 ± 0.034	1.358AsnGlu: 1.358 ± 0.032	1.045AsnPhe: 1.045 ± 0.03	2.602AsnGly: 2.602 ± 0.05	0.479AsnHis: 0.479 ± 0.021	1.503AsnIle: 1.503 ± 0.037	0.965AsnLys: 0.965 ± 0.03	2.584AsnLeu: 2.584 ± 0.042	0.659AsnMet: 0.659 ± 0.02	0.871AsnAsn: 0.871 ± 0.03	1.792AsnPro: 1.792 ± 0.042	0.771AsnGln: 0.771 ± 0.027	1.702AsnArg: 1.702 ± 0.039	1.306AsnSer: 1.306 ± 0.038	1.371AsnThr: 1.371 ± 0.039	2.237AsnVal: 2.237 ± 0.049	0.459AsnTrp: 0.459 ± 0.018	0.761AsnTyr: 0.761 ± 0.025	0.0AsnXaa: 0.0 ± 0.0
Pro	6.245ProAla: 6.245 ± 0.088	0.267ProCys: 0.267 ± 0.015	3.638ProAsp: 3.638 ± 0.064	3.026ProGlu: 3.026 ± 0.053	1.963ProPhe: 1.963 ± 0.039	4.257ProGly: 4.257 ± 0.06	1.055ProHis: 1.055 ± 0.028	2.499ProIle: 2.499 ± 0.039	2.002ProLys: 2.002 ± 0.043	4.624ProLeu: 4.624 ± 0.065	1.324ProMet: 1.324 ± 0.032	1.542ProAsn: 1.542 ± 0.034	2.58ProPro: 2.58 ± 0.062	1.76ProGln: 1.76 ± 0.037	2.917ProArg: 2.917 ± 0.055	2.946ProSer: 2.946 ± 0.058	2.756ProThr: 2.756 ± 0.049	4.127ProVal: 4.127 ± 0.06	0.704ProTrp: 0.704 ± 0.022	1.195ProTyr: 1.195 ± 0.027	0.0ProXaa: 0.0 ± 0.0
Gln	4.121GlnAla: 4.121 ± 0.063	0.227GlnCys: 0.227 ± 0.012	1.491GlnAsp: 1.491 ± 0.035	1.232GlnGlu: 1.232 ± 0.03	1.159GlnPhe: 1.159 ± 0.029	2.349GlnGly: 2.349 ± 0.045	0.676GlnHis: 0.676 ± 0.022	2.02GlnIle: 2.02 ± 0.038	1.196GlnLys: 1.196 ± 0.033	3.135GlnLeu: 3.135 ± 0.054	0.981GlnMet: 0.981 ± 0.032	0.908GlnAsn: 0.908 ± 0.026	1.926GlnPro: 1.926 ± 0.04	1.48GlnGln: 1.48 ± 0.036	2.577GlnArg: 2.577 ± 0.052	1.924GlnSer: 1.924 ± 0.042	1.835GlnThr: 1.835 ± 0.04	2.218GlnVal: 2.218 ± 0.045	0.443GlnTrp: 0.443 ± 0.018	0.642GlnTyr: 0.642 ± 0.024	0.0GlnXaa: 0.0 ± 0.0
Arg	7.479ArgAla: 7.479 ± 0.079	0.476ArgCys: 0.476 ± 0.021	4.068ArgAsp: 4.068 ± 0.055	3.518ArgGlu: 3.518 ± 0.058	2.618ArgPhe: 2.618 ± 0.052	4.656ArgGly: 4.656 ± 0.057	1.551ArgHis: 1.551 ± 0.036	3.869ArgIle: 3.869 ± 0.051	2.511ArgLys: 2.511 ± 0.048	6.723ArgLeu: 6.723 ± 0.084	1.887ArgMet: 1.887 ± 0.042	1.929ArgAsn: 1.929 ± 0.039	3.188ArgPro: 3.188 ± 0.058	2.249ArgGln: 2.249 ± 0.045	4.702ArgArg: 4.702 ± 0.077	3.599ArgSer: 3.599 ± 0.057	3.185ArgThr: 3.185 ± 0.054	4.526ArgVal: 4.526 ± 0.06	0.913ArgTrp: 0.913 ± 0.03	1.614ArgTyr: 1.614 ± 0.031	0.0ArgXaa: 0.0 ± 0.0
Ser	6.615SerAla: 6.615 ± 0.089	0.411SerCys: 0.411 ± 0.021	3.212SerAsp: 3.212 ± 0.05	2.737SerGlu: 2.737 ± 0.048	2.326SerPhe: 2.326 ± 0.038	5.445SerGly: 5.445 ± 0.078	1.134SerHis: 1.134 ± 0.027	3.091SerIle: 3.091 ± 0.055	1.974SerLys: 1.974 ± 0.044	5.337SerLeu: 5.337 ± 0.074	1.467SerMet: 1.467 ± 0.03	1.554SerAsn: 1.554 ± 0.038	2.837SerPro: 2.837 ± 0.052	1.657SerGln: 1.657 ± 0.037	3.429SerArg: 3.429 ± 0.05	3.376SerSer: 3.376 ± 0.068	2.875SerThr: 2.875 ± 0.058	4.233SerVal: 4.233 ± 0.07	0.719SerTrp: 0.719 ± 0.024	1.39SerTyr: 1.39 ± 0.031	0.0SerXaa: 0.0 ± 0.0
Thr	6.609ThrAla: 6.609 ± 0.08	0.414ThrCys: 0.414 ± 0.02	2.95ThrAsp: 2.95 ± 0.049	2.582ThrGlu: 2.582 ± 0.046	2.096ThrPhe: 2.096 ± 0.043	5.05ThrGly: 5.05 ± 0.071	1.01ThrHis: 1.01 ± 0.028	3.316ThrIle: 3.316 ± 0.061	1.868ThrLys: 1.868 ± 0.04	5.806ThrLeu: 5.806 ± 0.067	1.442ThrMet: 1.442 ± 0.035	1.431ThrAsn: 1.431 ± 0.034	3.459ThrPro: 3.459 ± 0.062	1.595ThrGln: 1.595 ± 0.032	3.34ThrArg: 3.34 ± 0.05	3.278ThrSer: 3.278 ± 0.063	3.234ThrThr: 3.234 ± 0.062	4.435ThrVal: 4.435 ± 0.058	0.65ThrTrp: 0.65 ± 0.023	1.235ThrTyr: 1.235 ± 0.03	0.0ThrXaa: 0.0 ± 0.0
Val	9.631ValAla: 9.631 ± 0.084	0.585ValCys: 0.585 ± 0.021	4.007ValAsp: 4.007 ± 0.055	3.971ValGlu: 3.971 ± 0.056	2.8ValPhe: 2.8 ± 0.045	5.745ValGly: 5.745 ± 0.074	1.393ValHis: 1.393 ± 0.035	4.544ValIle: 4.544 ± 0.07	2.802ValLys: 2.802 ± 0.048	7.392ValLeu: 7.392 ± 0.078	2.099ValMet: 2.099 ± 0.04	2.115ValAsn: 2.115 ± 0.047	3.742ValPro: 3.742 ± 0.051	2.199ValGln: 2.199 ± 0.041	4.389ValArg: 4.389 ± 0.061	4.464ValSer: 4.464 ± 0.065	4.737ValThr: 4.737 ± 0.059	6.343ValVal: 6.343 ± 0.069	0.847ValTrp: 0.847 ± 0.024	1.513ValTyr: 1.513 ± 0.033	0.0ValXaa: 0.0 ± 0.0
Trp	1.104TrpAla: 1.104 ± 0.028	0.137TrpCys: 0.137 ± 0.01	0.619TrpAsp: 0.619 ± 0.023	0.457TrpGlu: 0.457 ± 0.017	0.523TrpPhe: 0.523 ± 0.022	0.875TrpGly: 0.875 ± 0.031	0.321TrpHis: 0.321 ± 0.017	0.682TrpIle: 0.682 ± 0.023	0.481TrpLys: 0.481 ± 0.02	1.481TrpLeu: 1.481 ± 0.034	0.381TrpMet: 0.381 ± 0.016	0.43TrpAsn: 0.43 ± 0.019	0.7TrpPro: 0.7 ± 0.024	0.554TrpGln: 0.554 ± 0.022	1.037TrpArg: 1.037 ± 0.031	0.792TrpSer: 0.792 ± 0.028	0.828TrpThr: 0.828 ± 0.026	0.792TrpVal: 0.792 ± 0.024	0.215TrpTrp: 0.215 ± 0.014	0.288TrpTyr: 0.288 ± 0.015	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.614TyrAla: 2.614 ± 0.046	0.23TyrCys: 0.23 ± 0.013	1.429TyrAsp: 1.429 ± 0.034	1.095TyrGlu: 1.095 ± 0.029	0.924TyrPhe: 0.924 ± 0.026	2.197TyrGly: 2.197 ± 0.04	0.456TyrHis: 0.456 ± 0.021	0.957TyrIle: 0.957 ± 0.03	0.749TyrLys: 0.749 ± 0.028	2.373TyrLeu: 2.373 ± 0.042	0.446TyrMet: 0.446 ± 0.02	0.638TyrAsn: 0.638 ± 0.02	1.14TyrPro: 1.14 ± 0.026	0.767TyrGln: 0.767 ± 0.025	1.66TyrArg: 1.66 ± 0.03	1.146TyrSer: 1.146 ± 0.028	1.136TyrThr: 1.136 ± 0.026	1.678TyrVal: 1.678 ± 0.039	0.375TyrTrp: 0.375 ± 0.018	0.64TyrTyr: 0.64 ± 0.02	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4479 proteins (1310401 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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