Porphyromonas gingivalis (strain ATCC BAA-308 / W83)
Average proteome isoelectric point is 6.76
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1863 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q7MUS2|Q7MUS2_PORGI Fumarate hydratase class I OS=Porphyromonas gingivalis (strain ATCC BAA-308 / W83) OX=242619 GN=fumB PE=3 SV=1
MM1 pKa = 7.78 AYY3 pKa = 10.09 VINDD7 pKa = 3.34 SCVACGSCIDD17 pKa = 4.08 EE18 pKa = 4.96 CPVSAISEE26 pKa = 4.05 GSIYY30 pKa = 10.48 KK31 pKa = 9.72 IDD33 pKa = 4.41 ADD35 pKa = 3.96 TCIDD39 pKa = 4.16 CGTCAAACPSEE50 pKa = 4.79 AIHH53 pKa = 6.35 PAEE56 pKa = 4.02
Molecular weight: 5.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.918
IPC2_protein 3.732
IPC_protein 3.567
Toseland 3.401
ProMoST 3.732
Dawson 3.592
Bjellqvist 3.884
Wikipedia 3.567
Rodwell 3.427
Grimsley 3.325
Solomon 3.528
Lehninger 3.478
Nozaki 3.745
DTASelect 3.897
Thurlkill 3.49
EMBOSS 3.567
Sillero 3.706
Patrickios 0.006
IPC_peptide 3.528
IPC2_peptide 3.668
IPC2.peptide.svr19 3.772
Protein with the highest isoelectric point:
>tr|Q7MV44|Q7MV44_PORGI Ribonuclease Rne/Rng family OS=Porphyromonas gingivalis (strain ATCC BAA-308 / W83) OX=242619 GN=PG_1256 PE=4 SV=1
MM1 pKa = 7.82 PNGKK5 pKa = 9.01 KK6 pKa = 9.95 RR7 pKa = 11.84 KK8 pKa = 6.45 RR9 pKa = 11.84 HH10 pKa = 5.03 KK11 pKa = 10.05 MATHH15 pKa = 6.08 KK16 pKa = 10.38 RR17 pKa = 11.84 KK18 pKa = 9.82 KK19 pKa = 9.07 RR20 pKa = 11.84 LKK22 pKa = 10.27 KK23 pKa = 10.01 NRR25 pKa = 11.84 HH26 pKa = 5.04 KK27 pKa = 10.71 KK28 pKa = 9.82 KK29 pKa = 10.44 KK30 pKa = 9.85
Molecular weight: 3.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.408
IPC2_protein 10.965
IPC_protein 12.442
Toseland 12.647
ProMoST 13.1
Dawson 12.647
Bjellqvist 12.618
Wikipedia 13.1
Rodwell 12.735
Grimsley 12.691
Solomon 13.115
Lehninger 13.027
Nozaki 12.647
DTASelect 12.618
Thurlkill 12.647
EMBOSS 13.13
Sillero 12.647
Patrickios 12.457
IPC_peptide 13.13
IPC2_peptide 12.091
IPC2.peptide.svr19 8.929
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1863
0
1863
632208
30
2316
339.3
38.15
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.791 ± 0.052
1.169 ± 0.021
5.412 ± 0.034
6.718 ± 0.052
4.449 ± 0.036
6.947 ± 0.048
2.111 ± 0.022
6.768 ± 0.043
5.622 ± 0.052
9.782 ± 0.062
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.771 ± 0.028
4.032 ± 0.042
4.096 ± 0.034
3.288 ± 0.03
6.038 ± 0.048
6.413 ± 0.047
5.282 ± 0.04
6.39 ± 0.042
1.028 ± 0.02
3.897 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here