Amino acid dipepetide frequency for Geobacillus virus E2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.028AlaAla: 7.028 ± 1.759
0.937AlaCys: 0.937 ± 0.381
4.607AlaAsp: 4.607 ± 0.791
5.466AlaGlu: 5.466 ± 0.741
3.28AlaPhe: 3.28 ± 0.547
3.514AlaGly: 3.514 ± 0.457
0.859AlaHis: 0.859 ± 0.32
5.31AlaIle: 5.31 ± 0.895
6.169AlaLys: 6.169 ± 0.902
6.091AlaLeu: 6.091 ± 0.775
2.108AlaMet: 2.108 ± 0.398
4.295AlaAsn: 4.295 ± 1.281
1.171AlaPro: 1.171 ± 0.273
4.139AlaGln: 4.139 ± 0.839
3.826AlaArg: 3.826 ± 0.547
4.295AlaSer: 4.295 ± 0.587
4.607AlaThr: 4.607 ± 0.731
5.076AlaVal: 5.076 ± 1.015
1.015AlaTrp: 1.015 ± 0.296
2.265AlaTyr: 2.265 ± 0.48
0.0AlaXaa: 0.0 ± 0.0
Cys
0.547CysAla: 0.547 ± 0.236
0.156CysCys: 0.156 ± 0.159
0.625CysAsp: 0.625 ± 0.225
0.781CysGlu: 0.781 ± 0.293
0.469CysPhe: 0.469 ± 0.189
0.469CysGly: 0.469 ± 0.277
0.312CysHis: 0.312 ± 0.153
0.937CysIle: 0.937 ± 0.299
0.625CysLys: 0.625 ± 0.286
1.093CysLeu: 1.093 ± 0.328
0.156CysMet: 0.156 ± 0.106
0.625CysAsn: 0.625 ± 0.267
0.312CysPro: 0.312 ± 0.179
0.312CysGln: 0.312 ± 0.132
0.078CysArg: 0.078 ± 0.073
0.469CysSer: 0.469 ± 0.192
0.39CysThr: 0.39 ± 0.166
0.469CysVal: 0.469 ± 0.167
0.156CysTrp: 0.156 ± 0.096
0.547CysTyr: 0.547 ± 0.243
0.0CysXaa: 0.0 ± 0.0
Asp
2.889AspAla: 2.889 ± 0.588
0.859AspCys: 0.859 ± 0.318
3.592AspAsp: 3.592 ± 0.712
5.232AspGlu: 5.232 ± 0.677
2.811AspPhe: 2.811 ± 0.438
3.592AspGly: 3.592 ± 0.644
0.39AspHis: 0.39 ± 0.174
4.139AspIle: 4.139 ± 0.601
4.061AspLys: 4.061 ± 0.81
5.31AspLeu: 5.31 ± 0.819
1.484AspMet: 1.484 ± 0.398
3.045AspAsn: 3.045 ± 0.866
2.186AspPro: 2.186 ± 0.361
1.562AspGln: 1.562 ± 0.224
3.045AspArg: 3.045 ± 0.519
2.889AspSer: 2.889 ± 0.371
3.748AspThr: 3.748 ± 0.525
3.202AspVal: 3.202 ± 0.391
0.625AspTrp: 0.625 ± 0.251
2.733AspTyr: 2.733 ± 0.633
0.0AspXaa: 0.0 ± 0.0
Glu
5.935GluAla: 5.935 ± 0.687
0.547GluCys: 0.547 ± 0.211
2.889GluAsp: 2.889 ± 0.576
8.277GluGlu: 8.277 ± 1.005
2.811GluPhe: 2.811 ± 0.489
4.061GluGly: 4.061 ± 0.661
0.859GluHis: 0.859 ± 0.235
6.091GluIle: 6.091 ± 0.889
6.481GluLys: 6.481 ± 0.743
7.262GluLeu: 7.262 ± 0.697
2.577GluMet: 2.577 ± 0.527
4.139GluAsn: 4.139 ± 0.568
2.421GluPro: 2.421 ± 0.535
3.67GluGln: 3.67 ± 0.511
4.373GluArg: 4.373 ± 0.512
3.28GluSer: 3.28 ± 0.418
3.983GluThr: 3.983 ± 0.576
4.685GluVal: 4.685 ± 0.603
0.937GluTrp: 0.937 ± 0.233
4.295GluTyr: 4.295 ± 0.67
0.0GluXaa: 0.0 ± 0.0
Phe
3.202PheAla: 3.202 ± 0.432
0.625PheCys: 0.625 ± 0.222
2.655PheAsp: 2.655 ± 0.442
3.592PheGlu: 3.592 ± 0.528
2.343PhePhe: 2.343 ± 0.581
2.967PheGly: 2.967 ± 0.452
0.469PheHis: 0.469 ± 0.173
3.514PheIle: 3.514 ± 0.662
2.343PheLys: 2.343 ± 0.329
3.67PheLeu: 3.67 ± 0.574
0.781PheMet: 0.781 ± 0.3
1.562PheAsn: 1.562 ± 0.277
1.093PhePro: 1.093 ± 0.335
1.718PheGln: 1.718 ± 0.344
2.733PheArg: 2.733 ± 0.575
2.421PheSer: 2.421 ± 0.542
2.733PheThr: 2.733 ± 0.412
2.811PheVal: 2.811 ± 0.547
0.547PheTrp: 0.547 ± 0.204
1.718PheTyr: 1.718 ± 0.342
0.0PheXaa: 0.0 ± 0.0
Gly
4.451GlyAla: 4.451 ± 0.985
0.547GlyCys: 0.547 ± 0.274
3.592GlyAsp: 3.592 ± 0.955
4.763GlyGlu: 4.763 ± 0.773
2.421GlyPhe: 2.421 ± 0.408
3.514GlyGly: 3.514 ± 0.459
0.859GlyHis: 0.859 ± 0.29
4.763GlyIle: 4.763 ± 0.762
5.076GlyLys: 5.076 ± 0.583
4.92GlyLeu: 4.92 ± 0.536
2.265GlyMet: 2.265 ± 0.354
2.577GlyAsn: 2.577 ± 0.436
0.625GlyPro: 0.625 ± 0.207
2.108GlyGln: 2.108 ± 0.446
3.28GlyArg: 3.28 ± 0.735
2.967GlySer: 2.967 ± 0.441
3.436GlyThr: 3.436 ± 0.569
4.217GlyVal: 4.217 ± 0.588
1.484GlyTrp: 1.484 ± 0.414
2.655GlyTyr: 2.655 ± 0.452
0.0GlyXaa: 0.0 ± 0.0
His
0.39HisAla: 0.39 ± 0.161
0.156HisCys: 0.156 ± 0.106
0.937HisAsp: 0.937 ± 0.287
0.937HisGlu: 0.937 ± 0.34
0.781HisPhe: 0.781 ± 0.261
0.937HisGly: 0.937 ± 0.368
0.234HisHis: 0.234 ± 0.199
0.781HisIle: 0.781 ± 0.242
1.015HisLys: 1.015 ± 0.296
1.171HisLeu: 1.171 ± 0.315
0.39HisMet: 0.39 ± 0.179
0.859HisAsn: 0.859 ± 0.301
1.093HisPro: 1.093 ± 0.313
0.469HisGln: 0.469 ± 0.244
0.39HisArg: 0.39 ± 0.185
0.859HisSer: 0.859 ± 0.224
0.312HisThr: 0.312 ± 0.119
0.625HisVal: 0.625 ± 0.227
0.156HisTrp: 0.156 ± 0.092
1.328HisTyr: 1.328 ± 0.383
0.0HisXaa: 0.0 ± 0.0
Ile
6.013IleAla: 6.013 ± 0.645
0.547IleCys: 0.547 ± 0.221
5.154IleAsp: 5.154 ± 0.66
7.028IleGlu: 7.028 ± 0.687
2.265IlePhe: 2.265 ± 0.413
4.217IleGly: 4.217 ± 0.589
0.703IleHis: 0.703 ± 0.224
4.685IleIle: 4.685 ± 0.73
5.779IleLys: 5.779 ± 0.925
4.139IleLeu: 4.139 ± 0.794
1.562IleMet: 1.562 ± 0.379
2.811IleAsn: 2.811 ± 0.588
2.343IlePro: 2.343 ± 0.555
2.889IleGln: 2.889 ± 0.51
3.28IleArg: 3.28 ± 0.488
3.436IleSer: 3.436 ± 0.67
4.295IleThr: 4.295 ± 0.607
4.763IleVal: 4.763 ± 0.765
0.703IleTrp: 0.703 ± 0.209
2.733IleTyr: 2.733 ± 0.45
0.0IleXaa: 0.0 ± 0.0
Lys
4.529LysAla: 4.529 ± 0.712
0.234LysCys: 0.234 ± 0.123
3.67LysAsp: 3.67 ± 0.553
8.121LysGlu: 8.121 ± 1.146
2.811LysPhe: 2.811 ± 0.517
4.529LysGly: 4.529 ± 0.606
1.718LysHis: 1.718 ± 0.327
4.607LysIle: 4.607 ± 0.607
6.95LysLys: 6.95 ± 0.84
5.31LysLeu: 5.31 ± 0.687
3.045LysMet: 3.045 ± 0.489
4.685LysAsn: 4.685 ± 0.677
2.733LysPro: 2.733 ± 0.512
3.904LysGln: 3.904 ± 0.531
5.154LysArg: 5.154 ± 0.796
4.139LysSer: 4.139 ± 1.154
4.529LysThr: 4.529 ± 0.557
3.28LysVal: 3.28 ± 0.565
0.781LysTrp: 0.781 ± 0.221
2.811LysTyr: 2.811 ± 0.505
0.0LysXaa: 0.0 ± 0.0
Leu
6.716LeuAla: 6.716 ± 0.832
0.547LeuCys: 0.547 ± 0.229
4.529LeuAsp: 4.529 ± 0.608
5.7LeuGlu: 5.7 ± 0.728
3.67LeuPhe: 3.67 ± 0.781
5.232LeuGly: 5.232 ± 0.706
0.937LeuHis: 0.937 ± 0.275
4.92LeuIle: 4.92 ± 0.725
8.512LeuLys: 8.512 ± 0.704
7.965LeuLeu: 7.965 ± 0.92
2.108LeuMet: 2.108 ± 0.505
3.358LeuAsn: 3.358 ± 0.558
2.499LeuPro: 2.499 ± 0.455
4.295LeuGln: 4.295 ± 0.696
3.28LeuArg: 3.28 ± 0.534
4.998LeuSer: 4.998 ± 0.786
5.154LeuThr: 5.154 ± 0.544
4.217LeuVal: 4.217 ± 0.577
1.093LeuTrp: 1.093 ± 0.251
3.748LeuTyr: 3.748 ± 0.566
0.0LeuXaa: 0.0 ± 0.0
Met
2.186MetAla: 2.186 ± 0.49
0.156MetCys: 0.156 ± 0.099
1.328MetAsp: 1.328 ± 0.364
1.015MetGlu: 1.015 ± 0.316
0.937MetPhe: 0.937 ± 0.263
1.796MetGly: 1.796 ± 0.424
0.312MetHis: 0.312 ± 0.229
1.562MetIle: 1.562 ± 0.468
3.202MetLys: 3.202 ± 0.552
2.108MetLeu: 2.108 ± 0.3
1.015MetMet: 1.015 ± 0.371
1.328MetAsn: 1.328 ± 0.335
0.937MetPro: 0.937 ± 0.29
1.171MetGln: 1.171 ± 0.275
1.328MetArg: 1.328 ± 0.26
1.406MetSer: 1.406 ± 0.387
1.562MetThr: 1.562 ± 0.369
1.484MetVal: 1.484 ± 0.422
0.39MetTrp: 0.39 ± 0.155
1.015MetTyr: 1.015 ± 0.286
0.0MetXaa: 0.0 ± 0.0
Asn
5.232AsnAla: 5.232 ± 1.518
0.625AsnCys: 0.625 ± 0.277
2.889AsnAsp: 2.889 ± 0.575
3.436AsnGlu: 3.436 ± 0.453
1.562AsnPhe: 1.562 ± 0.349
5.076AsnGly: 5.076 ± 0.57
0.859AsnHis: 0.859 ± 0.257
2.967AsnIle: 2.967 ± 0.387
3.202AsnLys: 3.202 ± 0.561
3.904AsnLeu: 3.904 ± 0.586
0.781AsnMet: 0.781 ± 0.274
2.733AsnAsn: 2.733 ± 0.786
2.186AsnPro: 2.186 ± 0.509
1.874AsnGln: 1.874 ± 0.391
2.499AsnArg: 2.499 ± 0.442
2.265AsnSer: 2.265 ± 0.521
3.045AsnThr: 3.045 ± 0.7
2.967AsnVal: 2.967 ± 0.485
0.703AsnTrp: 0.703 ± 0.219
1.093AsnTyr: 1.093 ± 0.345
0.0AsnXaa: 0.0 ± 0.0
Pro
1.64ProAla: 1.64 ± 0.396
0.312ProCys: 0.312 ± 0.148
1.64ProAsp: 1.64 ± 0.434
1.952ProGlu: 1.952 ± 0.397
2.03ProPhe: 2.03 ± 0.494
1.562ProGly: 1.562 ± 0.303
0.781ProHis: 0.781 ± 0.254
2.811ProIle: 2.811 ± 0.507
2.108ProLys: 2.108 ± 0.442
2.343ProLeu: 2.343 ± 0.478
0.39ProMet: 0.39 ± 0.14
1.796ProAsn: 1.796 ± 0.49
1.015ProPro: 1.015 ± 0.27
0.937ProGln: 0.937 ± 0.253
1.562ProArg: 1.562 ± 0.4
2.577ProSer: 2.577 ± 0.426
2.03ProThr: 2.03 ± 0.417
1.874ProVal: 1.874 ± 0.373
0.234ProTrp: 0.234 ± 0.145
1.406ProTyr: 1.406 ± 0.402
0.0ProXaa: 0.0 ± 0.0
Gln
3.983GlnAla: 3.983 ± 0.643
0.39GlnCys: 0.39 ± 0.171
2.265GlnAsp: 2.265 ± 0.501
3.826GlnGlu: 3.826 ± 0.583
1.952GlnPhe: 1.952 ± 0.289
2.343GlnGly: 2.343 ± 0.526
0.312GlnHis: 0.312 ± 0.142
3.28GlnIle: 3.28 ± 0.504
3.358GlnLys: 3.358 ± 0.483
3.514GlnLeu: 3.514 ± 0.59
1.015GlnMet: 1.015 ± 0.313
1.952GlnAsn: 1.952 ± 0.432
1.328GlnPro: 1.328 ± 0.305
2.265GlnGln: 2.265 ± 0.467
2.03GlnArg: 2.03 ± 0.421
1.249GlnSer: 1.249 ± 0.274
2.186GlnThr: 2.186 ± 0.452
1.952GlnVal: 1.952 ± 0.363
0.547GlnTrp: 0.547 ± 0.165
1.952GlnTyr: 1.952 ± 0.389
0.0GlnXaa: 0.0 ± 0.0
Arg
2.967ArgAla: 2.967 ± 0.502
0.625ArgCys: 0.625 ± 0.217
2.733ArgAsp: 2.733 ± 0.532
4.529ArgGlu: 4.529 ± 0.691
3.124ArgPhe: 3.124 ± 0.58
2.577ArgGly: 2.577 ± 0.419
1.093ArgHis: 1.093 ± 0.274
3.28ArgIle: 3.28 ± 0.598
3.67ArgLys: 3.67 ± 0.756
5.232ArgLeu: 5.232 ± 0.627
1.562ArgMet: 1.562 ± 0.39
2.343ArgAsn: 2.343 ± 0.547
1.328ArgPro: 1.328 ± 0.454
2.499ArgGln: 2.499 ± 0.45
4.217ArgArg: 4.217 ± 0.814
3.514ArgSer: 3.514 ± 0.604
2.108ArgThr: 2.108 ± 0.414
3.748ArgVal: 3.748 ± 0.624
0.937ArgTrp: 0.937 ± 0.219
1.562ArgTyr: 1.562 ± 0.332
0.0ArgXaa: 0.0 ± 0.0
Ser
4.685SerAla: 4.685 ± 0.978
0.39SerCys: 0.39 ± 0.158
3.28SerAsp: 3.28 ± 0.584
2.733SerGlu: 2.733 ± 0.487
2.733SerPhe: 2.733 ± 0.437
2.889SerGly: 2.889 ± 0.665
0.547SerHis: 0.547 ± 0.179
4.685SerIle: 4.685 ± 0.62
2.889SerLys: 2.889 ± 0.399
4.998SerLeu: 4.998 ± 0.989
1.952SerMet: 1.952 ± 0.482
2.499SerAsn: 2.499 ± 0.415
2.265SerPro: 2.265 ± 0.614
1.406SerGln: 1.406 ± 0.352
2.889SerArg: 2.889 ± 0.423
3.514SerSer: 3.514 ± 0.707
3.202SerThr: 3.202 ± 0.48
3.904SerVal: 3.904 ± 0.615
0.625SerTrp: 0.625 ± 0.188
1.406SerTyr: 1.406 ± 0.29
0.0SerXaa: 0.0 ± 0.0
Thr
4.451ThrAla: 4.451 ± 0.551
0.39ThrCys: 0.39 ± 0.137
3.045ThrAsp: 3.045 ± 0.474
3.826ThrGlu: 3.826 ± 0.449
2.733ThrPhe: 2.733 ± 0.514
3.904ThrGly: 3.904 ± 0.595
0.859ThrHis: 0.859 ± 0.216
4.841ThrIle: 4.841 ± 0.575
4.139ThrLys: 4.139 ± 0.571
4.92ThrLeu: 4.92 ± 0.59
0.703ThrMet: 0.703 ± 0.215
3.124ThrAsn: 3.124 ± 0.632
2.577ThrPro: 2.577 ± 0.39
1.796ThrGln: 1.796 ± 0.43
2.499ThrArg: 2.499 ± 0.381
2.577ThrSer: 2.577 ± 0.577
3.436ThrThr: 3.436 ± 0.456
4.529ThrVal: 4.529 ± 0.471
0.859ThrTrp: 0.859 ± 0.257
2.265ThrTyr: 2.265 ± 0.445
0.0ThrXaa: 0.0 ± 0.0
Val
5.31ValAla: 5.31 ± 0.758
0.625ValCys: 0.625 ± 0.236
4.529ValAsp: 4.529 ± 0.615
3.904ValGlu: 3.904 ± 0.653
2.03ValPhe: 2.03 ± 0.413
3.28ValGly: 3.28 ± 0.42
1.015ValHis: 1.015 ± 0.306
3.124ValIle: 3.124 ± 0.587
4.685ValLys: 4.685 ± 0.494
4.998ValLeu: 4.998 ± 0.758
1.718ValMet: 1.718 ± 0.345
2.889ValAsn: 2.889 ± 0.556
1.796ValPro: 1.796 ± 0.438
2.265ValGln: 2.265 ± 0.433
3.748ValArg: 3.748 ± 0.635
3.826ValSer: 3.826 ± 0.861
3.983ValThr: 3.983 ± 0.667
4.139ValVal: 4.139 ± 0.707
1.328ValTrp: 1.328 ± 0.434
2.499ValTyr: 2.499 ± 0.517
0.0ValXaa: 0.0 ± 0.0
Trp
0.859TrpAla: 0.859 ± 0.292
0.234TrpCys: 0.234 ± 0.133
0.703TrpAsp: 0.703 ± 0.174
0.937TrpGlu: 0.937 ± 0.326
0.547TrpPhe: 0.547 ± 0.208
0.937TrpGly: 0.937 ± 0.218
0.234TrpHis: 0.234 ± 0.127
0.781TrpIle: 0.781 ± 0.216
0.781TrpLys: 0.781 ± 0.305
1.874TrpLeu: 1.874 ± 0.449
0.234TrpMet: 0.234 ± 0.133
1.171TrpAsn: 1.171 ± 0.407
0.0TrpPro: 0.0 ± 0.0
0.703TrpGln: 0.703 ± 0.206
0.781TrpArg: 0.781 ± 0.241
0.859TrpSer: 0.859 ± 0.249
0.234TrpThr: 0.234 ± 0.117
0.703TrpVal: 0.703 ± 0.318
0.156TrpTrp: 0.156 ± 0.086
0.937TrpTyr: 0.937 ± 0.302
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.045TyrAla: 3.045 ± 0.656
0.625TyrCys: 0.625 ± 0.231
2.733TyrAsp: 2.733 ± 0.49
3.514TyrGlu: 3.514 ± 0.706
2.186TyrPhe: 2.186 ± 0.389
3.202TyrGly: 3.202 ± 0.641
0.469TyrHis: 0.469 ± 0.197
2.265TyrIle: 2.265 ± 0.428
2.499TyrLys: 2.499 ± 0.493
2.655TyrLeu: 2.655 ± 0.504
0.312TyrMet: 0.312 ± 0.174
2.108TyrAsn: 2.108 ± 0.353
1.093TyrPro: 1.093 ± 0.329
1.718TyrGln: 1.718 ± 0.374
2.655TyrArg: 2.655 ± 0.502
1.952TyrSer: 1.952 ± 0.415
2.499TyrThr: 2.499 ± 0.392
3.045TyrVal: 3.045 ± 0.667
0.39TyrTrp: 0.39 ± 0.138
1.484TyrTyr: 1.484 ± 0.37
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 62 proteins (12807 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski