Amino acid dipepetide frequency for Azoarcus sp. (strain BH72)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	21.341AlaAla: 21.341 ± 0.231	1.412AlaCys: 1.412 ± 0.034	7.37AlaAsp: 7.37 ± 0.075	8.255AlaGlu: 8.255 ± 0.089	4.289AlaPhe: 4.289 ± 0.067	11.957AlaGly: 11.957 ± 0.119	2.793AlaHis: 2.793 ± 0.047	5.15AlaIle: 5.15 ± 0.076	3.023AlaLys: 3.023 ± 0.057	16.343AlaLeu: 16.343 ± 0.165	3.078AlaMet: 3.078 ± 0.048	2.757AlaAsn: 2.757 ± 0.049	6.704AlaPro: 6.704 ± 0.094	4.494AlaGln: 4.494 ± 0.062	10.859AlaArg: 10.859 ± 0.116	5.911AlaSer: 5.911 ± 0.071	5.928AlaThr: 5.928 ± 0.073	9.686AlaVal: 9.686 ± 0.104	1.88AlaTrp: 1.88 ± 0.038	2.505AlaTyr: 2.505 ± 0.048	0.0AlaXaa: 0.0 ± 0.0
Cys	1.261CysAla: 1.261 ± 0.029	0.149CysCys: 0.149 ± 0.011	0.545CysAsp: 0.545 ± 0.022	0.547CysGlu: 0.547 ± 0.02	0.345CysPhe: 0.345 ± 0.018	1.086CysGly: 1.086 ± 0.027	0.315CysHis: 0.315 ± 0.016	0.398CysIle: 0.398 ± 0.019	0.222CysLys: 0.222 ± 0.012	0.871CysLeu: 0.871 ± 0.027	0.171CysMet: 0.171 ± 0.012	0.247CysAsn: 0.247 ± 0.012	0.547CysPro: 0.547 ± 0.021	0.216CysGln: 0.216 ± 0.014	0.707CysArg: 0.707 ± 0.022	0.506CysSer: 0.506 ± 0.021	0.46CysThr: 0.46 ± 0.021	0.737CysVal: 0.737 ± 0.027	0.127CysTrp: 0.127 ± 0.01	0.248CysTyr: 0.248 ± 0.015	0.0CysXaa: 0.0 ± 0.0
Asp	7.439AspAla: 7.439 ± 0.084	0.543AspCys: 0.543 ± 0.021	3.146AspAsp: 3.146 ± 0.056	3.649AspGlu: 3.649 ± 0.064	2.305AspPhe: 2.305 ± 0.044	5.056AspGly: 5.056 ± 0.073	1.23AspHis: 1.23 ± 0.031	2.57AspIle: 2.57 ± 0.05	1.612AspLys: 1.612 ± 0.042	5.487AspLeu: 5.487 ± 0.069	1.096AspMet: 1.096 ± 0.033	1.313AspAsn: 1.313 ± 0.034	3.114AspPro: 3.114 ± 0.05	1.396AspGln: 1.396 ± 0.037	3.722AspArg: 3.722 ± 0.055	2.279AspSer: 2.279 ± 0.042	2.602AspThr: 2.602 ± 0.045	3.774AspVal: 3.774 ± 0.055	1.002AspTrp: 1.002 ± 0.027	1.583AspTyr: 1.583 ± 0.037	0.0AspXaa: 0.0 ± 0.0
Glu	8.067GluAla: 8.067 ± 0.089	0.459GluCys: 0.459 ± 0.019	2.67GluAsp: 2.67 ± 0.052	2.888GluGlu: 2.888 ± 0.053	1.85GluPhe: 1.85 ± 0.039	4.282GluGly: 4.282 ± 0.058	1.445GluHis: 1.445 ± 0.036	3.013GluIle: 3.013 ± 0.052	1.773GluLys: 1.773 ± 0.048	6.395GluLeu: 6.395 ± 0.071	1.292GluMet: 1.292 ± 0.033	1.368GluAsn: 1.368 ± 0.029	2.57GluPro: 2.57 ± 0.051	2.423GluGln: 2.423 ± 0.044	5.589GluArg: 5.589 ± 0.077	2.418GluSer: 2.418 ± 0.046	2.852GluThr: 2.852 ± 0.059	4.53GluVal: 4.53 ± 0.069	0.838GluTrp: 0.838 ± 0.027	1.122GluTyr: 1.122 ± 0.029	0.0GluXaa: 0.0 ± 0.0
Phe	4.472PheAla: 4.472 ± 0.061	0.402PheCys: 0.402 ± 0.017	2.533PheAsp: 2.533 ± 0.043	2.174PheGlu: 2.174 ± 0.041	1.34PhePhe: 1.34 ± 0.037	3.555PheGly: 3.555 ± 0.06	0.86PheHis: 0.86 ± 0.022	1.489PheIle: 1.489 ± 0.037	0.899PheLys: 0.899 ± 0.031	3.144PheLeu: 3.144 ± 0.054	0.722PheMet: 0.722 ± 0.025	1.048PheAsn: 1.048 ± 0.026	1.61PhePro: 1.61 ± 0.034	0.991PheGln: 0.991 ± 0.031	2.318PheArg: 2.318 ± 0.039	2.092PheSer: 2.092 ± 0.041	1.672PheThr: 1.672 ± 0.035	2.847PheVal: 2.847 ± 0.049	0.452PheTrp: 0.452 ± 0.022	0.853PheTyr: 0.853 ± 0.029	0.0PheXaa: 0.0 ± 0.0
Gly	9.756GlyAla: 9.756 ± 0.107	0.991GlyCys: 0.991 ± 0.029	4.433GlyAsp: 4.433 ± 0.062	5.562GlyGlu: 5.562 ± 0.074	3.373GlyPhe: 3.373 ± 0.056	7.426GlyGly: 7.426 ± 0.108	2.005GlyHis: 2.005 ± 0.044	3.957GlyIle: 3.957 ± 0.064	2.984GlyLys: 2.984 ± 0.058	9.028GlyLeu: 9.028 ± 0.102	2.272GlyMet: 2.272 ± 0.045	2.082GlyAsn: 2.082 ± 0.043	2.985GlyPro: 2.985 ± 0.049	2.781GlyGln: 2.781 ± 0.064	6.744GlyArg: 6.744 ± 0.073	4.295GlySer: 4.295 ± 0.062	4.05GlyThr: 4.05 ± 0.072	6.594GlyVal: 6.594 ± 0.076	1.442GlyTrp: 1.442 ± 0.038	2.357GlyTyr: 2.357 ± 0.042	0.0GlyXaa: 0.0 ± 0.0
His	2.932HisAla: 2.932 ± 0.056	0.294HisCys: 0.294 ± 0.017	1.315HisAsp: 1.315 ± 0.033	1.122HisGlu: 1.122 ± 0.031	0.945HisPhe: 0.945 ± 0.027	2.245HisGly: 2.245 ± 0.044	0.667HisHis: 0.667 ± 0.024	0.912HisIle: 0.912 ± 0.025	0.501HisLys: 0.501 ± 0.022	2.414HisLeu: 2.414 ± 0.045	0.392HisMet: 0.392 ± 0.016	0.496HisAsn: 0.496 ± 0.017	1.653HisPro: 1.653 ± 0.039	0.662HisGln: 0.662 ± 0.023	1.672HisArg: 1.672 ± 0.042	0.952HisSer: 0.952 ± 0.03	0.991HisThr: 0.991 ± 0.027	1.487HisVal: 1.487 ± 0.033	0.353HisTrp: 0.353 ± 0.016	0.716HisTyr: 0.716 ± 0.023	0.0HisXaa: 0.0 ± 0.0
Ile	6.195IleAla: 6.195 ± 0.083	0.4IleCys: 0.4 ± 0.017	3.077IleAsp: 3.077 ± 0.049	3.143IleGlu: 3.143 ± 0.056	1.24IlePhe: 1.24 ± 0.033	4.21IleGly: 4.21 ± 0.062	0.953IleHis: 0.953 ± 0.027	1.51IleIle: 1.51 ± 0.038	1.263IleLys: 1.263 ± 0.032	3.466IleLeu: 3.466 ± 0.05	0.667IleMet: 0.667 ± 0.024	1.314IleAsn: 1.314 ± 0.033	2.042IlePro: 2.042 ± 0.039	1.111IleGln: 1.111 ± 0.031	2.962IleArg: 2.962 ± 0.046	2.126IleSer: 2.126 ± 0.043	2.196IleThr: 2.196 ± 0.046	3.4IleVal: 3.4 ± 0.051	0.426IleTrp: 0.426 ± 0.018	0.963IleTyr: 0.963 ± 0.027	0.0IleXaa: 0.0 ± 0.0
Lys	3.321LysAla: 3.321 ± 0.059	0.175LysCys: 0.175 ± 0.014	1.454LysAsp: 1.454 ± 0.04	1.304LysGlu: 1.304 ± 0.032	0.715LysPhe: 0.715 ± 0.022	2.131LysGly: 2.131 ± 0.046	0.579LysHis: 0.579 ± 0.024	1.289LysIle: 1.289 ± 0.034	1.049LysLys: 1.049 ± 0.038	3.069LysLeu: 3.069 ± 0.057	0.614LysMet: 0.614 ± 0.022	0.799LysAsn: 0.799 ± 0.027	1.787LysPro: 1.787 ± 0.04	0.997LysGln: 0.997 ± 0.031	1.99LysArg: 1.99 ± 0.045	1.398LysSer: 1.398 ± 0.034	1.478LysThr: 1.478 ± 0.037	2.301LysVal: 2.301 ± 0.048	0.318LysTrp: 0.318 ± 0.017	0.602LysTyr: 0.602 ± 0.023	0.0LysXaa: 0.0 ± 0.0
Leu	16.97LeuAla: 16.97 ± 0.151	1.004LeuCys: 1.004 ± 0.03	6.44LeuAsp: 6.44 ± 0.082	5.917LeuGlu: 5.917 ± 0.069	3.679LeuPhe: 3.679 ± 0.06	9.094LeuGly: 9.094 ± 0.097	2.325LeuHis: 2.325 ± 0.049	4.365LeuIle: 4.365 ± 0.061	3.082LeuLys: 3.082 ± 0.052	11.745LeuLeu: 11.745 ± 0.136	2.226LeuMet: 2.226 ± 0.044	2.532LeuAsn: 2.532 ± 0.05	6.656LeuPro: 6.656 ± 0.079	3.691LeuGln: 3.691 ± 0.061	8.728LeuArg: 8.728 ± 0.101	5.395LeuSer: 5.395 ± 0.069	5.178LeuThr: 5.178 ± 0.058	7.951LeuVal: 7.951 ± 0.081	1.258LeuTrp: 1.258 ± 0.035	2.242LeuTyr: 2.242 ± 0.044	0.0LeuXaa: 0.0 ± 0.0
Met	2.551MetAla: 2.551 ± 0.044	0.158MetCys: 0.158 ± 0.011	1.056MetAsp: 1.056 ± 0.032	1.083MetGlu: 1.083 ± 0.028	0.618MetPhe: 0.618 ± 0.021	1.503MetGly: 1.503 ± 0.037	0.48MetHis: 0.48 ± 0.021	0.907MetIle: 0.907 ± 0.024	0.937MetLys: 0.937 ± 0.025	2.508MetLeu: 2.508 ± 0.041	0.528MetMet: 0.528 ± 0.023	0.801MetAsn: 0.801 ± 0.026	1.321MetPro: 1.321 ± 0.032	0.879MetGln: 0.879 ± 0.03	1.626MetArg: 1.626 ± 0.031	1.463MetSer: 1.463 ± 0.037	1.342MetThr: 1.342 ± 0.032	1.545MetVal: 1.545 ± 0.036	0.198MetTrp: 0.198 ± 0.014	0.345MetTyr: 0.345 ± 0.015	0.0MetXaa: 0.0 ± 0.0
Asn	3.027AsnAla: 3.027 ± 0.049	0.287AsnCys: 0.287 ± 0.014	1.227AsnAsp: 1.227 ± 0.03	1.156AsnGlu: 1.156 ± 0.028	0.882AsnPhe: 0.882 ± 0.026	2.062AsnGly: 2.062 ± 0.043	0.517AsnHis: 0.517 ± 0.021	1.139AsnIle: 1.139 ± 0.035	0.661AsnLys: 0.661 ± 0.025	2.625AsnLeu: 2.625 ± 0.05	0.479AsnMet: 0.479 ± 0.019	0.658AsnAsn: 0.658 ± 0.024	1.781AsnPro: 1.781 ± 0.035	0.754AsnGln: 0.754 ± 0.025	1.839AsnArg: 1.839 ± 0.042	1.031AsnSer: 1.031 ± 0.03	1.248AsnThr: 1.248 ± 0.034	1.72AsnVal: 1.72 ± 0.041	0.389AsnTrp: 0.389 ± 0.015	0.639AsnTyr: 0.639 ± 0.02	0.0AsnXaa: 0.0 ± 0.0
Pro	8.355ProAla: 8.355 ± 0.112	0.398ProCys: 0.398 ± 0.016	3.314ProAsp: 3.314 ± 0.051	3.465ProGlu: 3.465 ± 0.058	1.813ProPhe: 1.813 ± 0.032	4.588ProGly: 4.588 ± 0.054	1.088ProHis: 1.088 ± 0.029	1.791ProIle: 1.791 ± 0.034	1.209ProLys: 1.209 ± 0.034	5.706ProLeu: 5.706 ± 0.069	1.034ProMet: 1.034 ± 0.031	1.166ProAsn: 1.166 ± 0.03	3.25ProPro: 3.25 ± 0.07	1.933ProGln: 1.933 ± 0.036	3.51ProArg: 3.51 ± 0.054	2.257ProSer: 2.257 ± 0.043	2.325ProThr: 2.325 ± 0.048	4.283ProVal: 4.283 ± 0.059	0.752ProTrp: 0.752 ± 0.027	1.149ProTyr: 1.149 ± 0.028	0.0ProXaa: 0.0 ± 0.0
Gln	4.517GlnAla: 4.517 ± 0.053	0.257GlnCys: 0.257 ± 0.015	1.44GlnAsp: 1.44 ± 0.034	1.411GlnGlu: 1.411 ± 0.04	1.08GlnPhe: 1.08 ± 0.031	2.553GlnGly: 2.553 ± 0.047	0.836GlnHis: 0.836 ± 0.025	1.515GlnIle: 1.515 ± 0.032	0.887GlnLys: 0.887 ± 0.028	3.797GlnLeu: 3.797 ± 0.057	0.799GlnMet: 0.799 ± 0.026	0.673GlnAsn: 0.673 ± 0.025	2.046GlnPro: 2.046 ± 0.043	1.608GlnGln: 1.608 ± 0.043	3.09GlnArg: 3.09 ± 0.053	1.512GlnSer: 1.512 ± 0.038	1.554GlnThr: 1.554 ± 0.037	2.515GlnVal: 2.515 ± 0.046	0.501GlnTrp: 0.501 ± 0.019	0.67GlnTyr: 0.67 ± 0.024	0.0GlnXaa: 0.0 ± 0.0
Arg	9.222ArgAla: 9.222 ± 0.095	0.709ArgCys: 0.709 ± 0.021	4.144ArgAsp: 4.144 ± 0.057	4.884ArgGlu: 4.884 ± 0.067	3.317ArgPhe: 3.317 ± 0.054	5.356ArgGly: 5.356 ± 0.072	2.119ArgHis: 2.119 ± 0.041	3.903ArgIle: 3.903 ± 0.064	1.931ArgLys: 1.931 ± 0.043	9.442ArgLeu: 9.442 ± 0.094	1.955ArgMet: 1.955 ± 0.033	1.984ArgAsn: 1.984 ± 0.039	3.768ArgPro: 3.768 ± 0.053	2.797ArgGln: 2.797 ± 0.051	6.557ArgArg: 6.557 ± 0.081	3.695ArgSer: 3.695 ± 0.056	3.144ArgThr: 3.144 ± 0.048	5.619ArgVal: 5.619 ± 0.077	1.169ArgTrp: 1.169 ± 0.027	2.159ArgTyr: 2.159 ± 0.039	0.0ArgXaa: 0.0 ± 0.0
Ser	6.318SerAla: 6.318 ± 0.083	0.448SerCys: 0.448 ± 0.021	2.255SerAsp: 2.255 ± 0.041	2.291SerGlu: 2.291 ± 0.047	1.924SerPhe: 1.924 ± 0.041	4.907SerGly: 4.907 ± 0.07	1.05SerHis: 1.05 ± 0.029	2.1SerIle: 2.1 ± 0.04	1.092SerLys: 1.092 ± 0.031	5.199SerLeu: 5.199 ± 0.065	1.093SerMet: 1.093 ± 0.027	1.139SerAsn: 1.139 ± 0.032	2.633SerPro: 2.633 ± 0.05	1.405SerGln: 1.405 ± 0.032	3.596SerArg: 3.596 ± 0.063	2.412SerSer: 2.412 ± 0.048	2.377SerThr: 2.377 ± 0.049	3.561SerVal: 3.561 ± 0.057	0.644SerTrp: 0.644 ± 0.021	1.145SerTyr: 1.145 ± 0.03	0.0SerXaa: 0.0 ± 0.0
Thr	5.905ThrAla: 5.905 ± 0.078	0.424ThrCys: 0.424 ± 0.018	2.429ThrAsp: 2.429 ± 0.044	2.484ThrGlu: 2.484 ± 0.045	1.497ThrPhe: 1.497 ± 0.032	4.233ThrGly: 4.233 ± 0.059	1.068ThrHis: 1.068 ± 0.027	1.853ThrIle: 1.853 ± 0.039	0.97ThrLys: 0.97 ± 0.033	6.167ThrLeu: 6.167 ± 0.079	0.847ThrMet: 0.847 ± 0.027	0.973ThrAsn: 0.973 ± 0.033	3.25ThrPro: 3.25 ± 0.049	1.399ThrGln: 1.399 ± 0.036	3.54ThrArg: 3.54 ± 0.05	2.073ThrSer: 2.073 ± 0.043	2.468ThrThr: 2.468 ± 0.061	4.191ThrVal: 4.191 ± 0.082	0.588ThrTrp: 0.588 ± 0.021	0.992ThrTyr: 0.992 ± 0.026	0.0ThrXaa: 0.0 ± 0.0
Val	10.129ValAla: 10.129 ± 0.1	0.778ValCys: 0.778 ± 0.027	4.163ValAsp: 4.163 ± 0.058	4.766ValGlu: 4.766 ± 0.067	2.843ValPhe: 2.843 ± 0.047	5.88ValGly: 5.88 ± 0.069	1.444ValHis: 1.444 ± 0.038	3.419ValIle: 3.419 ± 0.055	2.238ValLys: 2.238 ± 0.055	8.54ValLeu: 8.54 ± 0.083	1.797ValMet: 1.797 ± 0.042	1.757ValAsn: 1.757 ± 0.035	3.823ValPro: 3.823 ± 0.059	2.278ValGln: 2.278 ± 0.044	5.53ValArg: 5.53 ± 0.063	3.952ValSer: 3.952 ± 0.053	3.747ValThr: 3.747 ± 0.068	6.606ValVal: 6.606 ± 0.086	0.918ValTrp: 0.918 ± 0.029	1.42ValTyr: 1.42 ± 0.035	0.0ValXaa: 0.0 ± 0.0
Trp	1.335TrpAla: 1.335 ± 0.034	0.143TrpCys: 0.143 ± 0.009	0.628TrpAsp: 0.628 ± 0.023	0.61TrpGlu: 0.61 ± 0.023	0.511TrpPhe: 0.511 ± 0.022	0.903TrpGly: 0.903 ± 0.029	0.367TrpHis: 0.367 ± 0.016	0.605TrpIle: 0.605 ± 0.021	0.428TrpLys: 0.428 ± 0.019	2.043TrpLeu: 2.043 ± 0.044	0.376TrpMet: 0.376 ± 0.017	0.4TrpAsn: 0.4 ± 0.017	0.67TrpPro: 0.67 ± 0.024	0.66TrpGln: 0.66 ± 0.024	1.273TrpArg: 1.273 ± 0.035	0.7TrpSer: 0.7 ± 0.023	0.637TrpThr: 0.637 ± 0.024	1.032TrpVal: 1.032 ± 0.031	0.25TrpTrp: 0.25 ± 0.015	0.296TrpTyr: 0.296 ± 0.016	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.623TyrAla: 2.623 ± 0.048	0.273TyrCys: 0.273 ± 0.015	1.281TyrAsp: 1.281 ± 0.035	1.132TyrGlu: 1.132 ± 0.032	0.907TyrPhe: 0.907 ± 0.025	2.087TyrGly: 2.087 ± 0.052	0.581TyrHis: 0.581 ± 0.02	0.779TyrIle: 0.779 ± 0.027	0.608TyrLys: 0.608 ± 0.023	2.488TyrLeu: 2.488 ± 0.043	0.412TyrMet: 0.412 ± 0.018	0.613TyrAsn: 0.613 ± 0.022	1.175TyrPro: 1.175 ± 0.03	0.785TyrGln: 0.785 ± 0.024	2.024TyrArg: 2.024 ± 0.042	1.12TyrSer: 1.12 ± 0.03	1.103TyrThr: 1.103 ± 0.031	1.65TyrVal: 1.65 ± 0.037	0.362TyrTrp: 0.362 ± 0.016	0.65TyrTyr: 0.65 ± 0.022	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3981 proteins (1326302 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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