Weizmannia ginsengihum
Average proteome isoelectric point is 6.56
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2914 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0A6Y1J2|A0A0A6Y1J2_9BACI Ribosome biogenesis GTPase A OS=Weizmannia ginsengihum OX=363870 GN=ylqF PE=3 SV=1
MM1 pKa = 7.64 AKK3 pKa = 8.8 YY4 pKa = 9.02 TIVDD8 pKa = 3.75 RR9 pKa = 11.84 EE10 pKa = 4.13 TCIACGSCEE19 pKa = 3.87 AVAPDD24 pKa = 3.37 IYY26 pKa = 10.91 GYY28 pKa = 10.69 DD29 pKa = 3.54 DD30 pKa = 3.73 EE31 pKa = 6.65 GISFVLLDD39 pKa = 4.27 DD40 pKa = 4.37 NKK42 pKa = 10.05 GTEE45 pKa = 4.0 AVPDD49 pKa = 3.86 EE50 pKa = 5.23 FIDD53 pKa = 5.01 DD54 pKa = 3.52 IVDD57 pKa = 3.49 AFEE60 pKa = 4.72 GCPSEE65 pKa = 4.46 SIKK68 pKa = 10.85 IADD71 pKa = 4.15 RR72 pKa = 11.84 PFNGNPHH79 pKa = 6.48 AFEE82 pKa = 4.26
Molecular weight: 8.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.724
IPC2_protein 3.795
IPC_protein 3.745
Toseland 3.541
ProMoST 3.884
Dawson 3.745
Bjellqvist 3.961
Wikipedia 3.681
Rodwell 3.579
Grimsley 3.452
Solomon 3.719
Lehninger 3.681
Nozaki 3.859
DTASelect 4.075
Thurlkill 3.605
EMBOSS 3.694
Sillero 3.872
Patrickios 0.846
IPC_peptide 3.719
IPC2_peptide 3.846
IPC2.peptide.svr19 3.803
Protein with the highest isoelectric point:
>tr|A0A0A6VEN8|A0A0A6VEN8_9BACI Ribonuclease M5 OS=Weizmannia ginsengihum OX=363870 GN=rnmV PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.42 RR3 pKa = 11.84 TYY5 pKa = 10.34 QPNKK9 pKa = 8.21 RR10 pKa = 11.84 KK11 pKa = 9.62 RR12 pKa = 11.84 SKK14 pKa = 9.83 VHH16 pKa = 5.77 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MSTKK25 pKa = 10.19 NGRR28 pKa = 11.84 KK29 pKa = 8.86 VLASRR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 8.73 GRR39 pKa = 11.84 KK40 pKa = 8.76 VLSAA44 pKa = 4.05
Molecular weight: 5.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.415
IPC2_protein 11.096
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.398
Grimsley 12.661
Solomon 13.115
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.135
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.013
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2914
0
2914
806891
26
2386
276.9
31.11
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.042 ± 0.048
0.814 ± 0.012
4.921 ± 0.039
7.049 ± 0.062
4.434 ± 0.037
6.747 ± 0.043
2.361 ± 0.021
8.483 ± 0.044
7.108 ± 0.047
9.604 ± 0.055
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.741 ± 0.019
4.486 ± 0.03
3.544 ± 0.027
3.981 ± 0.035
3.988 ± 0.03
5.91 ± 0.037
5.394 ± 0.031
6.91 ± 0.037
1.001 ± 0.018
3.485 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here