Bacillus phage Shbh1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Herelleviridae; Bastillevirinae; Shalavirus; Bacillus virus Shbh1

Average proteome isoelectric point is 5.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 177 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A142F1C6|A0A142F1C6_9CAUD Putative membrane protein OS=Bacillus phage Shbh1 OX=1796992 PE=4 SV=1
MM1 pKa = 8.06PYY3 pKa = 10.89GLNEE7 pKa = 5.18DD8 pKa = 3.96EE9 pKa = 4.3LTTYY13 pKa = 9.82KK14 pKa = 10.6QLVQLYY20 pKa = 8.88TNEE23 pKa = 4.88GILPSVTEE31 pKa = 5.05SIPFMIPTNKK41 pKa = 9.84HH42 pKa = 5.33GEE44 pKa = 3.91PDD46 pKa = 3.62EE47 pKa = 4.49VFLASLLKK55 pKa = 10.62DD56 pKa = 3.44VEE58 pKa = 4.43LSVTLSVVYY67 pKa = 9.67MSLEE71 pKa = 4.03YY72 pKa = 10.92ADD74 pKa = 5.0LQCSDD79 pKa = 3.99KK80 pKa = 11.41SEE82 pKa = 4.05EE83 pKa = 4.94DD84 pKa = 2.9IVEE87 pKa = 4.16YY88 pKa = 11.03LRR90 pKa = 11.84VKK92 pKa = 10.2YY93 pKa = 10.29EE94 pKa = 4.16NYY96 pKa = 10.71LSTEE100 pKa = 3.81LLNRR104 pKa = 11.84RR105 pKa = 11.84VCFSWEE111 pKa = 4.11TVSDD115 pKa = 4.21VIIEE119 pKa = 4.28ALLEE123 pKa = 4.02LPYY126 pKa = 10.02MYY128 pKa = 10.96VNSVEE133 pKa = 4.2NLEE136 pKa = 3.98FDD138 pKa = 3.3EE139 pKa = 6.24DD140 pKa = 3.74KK141 pKa = 11.05FLEE144 pKa = 4.29EE145 pKa = 5.18RR146 pKa = 11.84LLAYY150 pKa = 10.1DD151 pKa = 4.39KK152 pKa = 11.4YY153 pKa = 10.19ITLEE157 pKa = 3.98FLDD160 pKa = 4.55EE161 pKa = 4.32EE162 pKa = 4.74DD163 pKa = 4.58FSEE166 pKa = 4.47EE167 pKa = 4.72DD168 pKa = 3.94EE169 pKa = 4.94EE170 pKa = 4.76EE171 pKa = 4.29DD172 pKa = 5.59DD173 pKa = 4.32YY174 pKa = 12.15FEE176 pKa = 4.45

Molecular weight: 20.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A142F1B9|A0A142F1B9_9CAUD Uncharacterized protein OS=Bacillus phage Shbh1 OX=1796992 PE=4 SV=1
MM1 pKa = 7.35TKK3 pKa = 10.04IFIDD7 pKa = 4.02PGHH10 pKa = 6.79GGRR13 pKa = 11.84DD14 pKa = 3.36PGAVGNGLKK23 pKa = 10.61EE24 pKa = 4.01KK25 pKa = 10.79DD26 pKa = 3.37LTLDD30 pKa = 3.54ISRR33 pKa = 11.84KK34 pKa = 9.53LDD36 pKa = 3.11TYY38 pKa = 11.23LRR40 pKa = 11.84NNYY43 pKa = 9.84QGVQTRR49 pKa = 11.84LSRR52 pKa = 11.84TNDD55 pKa = 3.08TFVSLTARR63 pKa = 11.84ADD65 pKa = 3.37AANNWGADD73 pKa = 3.08IFVSIHH79 pKa = 5.19VNAGGGTGFEE89 pKa = 4.23TLINGTLSLNSTTGRR104 pKa = 11.84IAKK107 pKa = 8.54EE108 pKa = 3.89FNDD111 pKa = 3.45EE112 pKa = 4.19VVNQLLQNRR121 pKa = 11.84SGWGIVNRR129 pKa = 11.84GVKK132 pKa = 8.29NQRR135 pKa = 11.84NLTVLQRR142 pKa = 11.84TRR144 pKa = 11.84MSAILSEE151 pKa = 4.32SLFIDD156 pKa = 3.7RR157 pKa = 11.84ADD159 pKa = 4.13DD160 pKa = 4.24ARR162 pKa = 11.84LLKK165 pKa = 10.62NRR167 pKa = 11.84AFIDD171 pKa = 3.65ALVEE175 pKa = 3.87AHH177 pKa = 6.87AKK179 pKa = 10.34ALEE182 pKa = 4.19KK183 pKa = 10.75VLKK186 pKa = 10.43LKK188 pKa = 10.71RR189 pKa = 11.84KK190 pKa = 7.38STNNSGSSNSGSSNSGSSSPSSNGSSNSGSSRR222 pKa = 11.84PRR224 pKa = 11.84KK225 pKa = 9.45FNLPNAVYY233 pKa = 10.15RR234 pKa = 11.84PNRR237 pKa = 11.84PFPRR241 pKa = 11.84GSGVRR246 pKa = 11.84TIQNALVSVYY256 pKa = 8.99FFPDD260 pKa = 2.88SSAPNRR266 pKa = 11.84GVDD269 pKa = 3.69GVFGPKK275 pKa = 9.62TSDD278 pKa = 2.71AVRR281 pKa = 11.84RR282 pKa = 11.84FQLMYY287 pKa = 9.42MGSSEE292 pKa = 4.52ADD294 pKa = 3.64GIYY297 pKa = 10.66GPRR300 pKa = 11.84TRR302 pKa = 11.84AALDD306 pKa = 4.01RR307 pKa = 11.84IVNKK311 pKa = 10.55

Molecular weight: 33.73 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

177

0

177

39079

40

1511

220.8

25.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.588 ± 0.235

0.699 ± 0.064

6.666 ± 0.154

8.291 ± 0.252

4.066 ± 0.109

5.983 ± 0.286

2.078 ± 0.1

6.702 ± 0.168

7.393 ± 0.337

8.57 ± 0.217

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.275 ± 0.104

5.704 ± 0.228

3.222 ± 0.158

3.782 ± 0.17

4.849 ± 0.209

6.927 ± 0.2

5.816 ± 0.188

6.95 ± 0.171

0.983 ± 0.054

4.458 ± 0.146
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski