Hubei narna-like virus 13

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 9.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KIH2|A0A1L3KIH2_9VIRU RNA-dependent RNA polymerase OS=Hubei narna-like virus 13 OX=1922943 PE=4 SV=1
MM1 pKa = 7.7AISTPVTRR9 pKa = 11.84GGLRR13 pKa = 11.84KK14 pKa = 9.43QLEE17 pKa = 4.05GLARR21 pKa = 11.84GTGFLTRR28 pKa = 11.84SGCVVFAARR37 pKa = 11.84LASARR42 pKa = 11.84SFRR45 pKa = 11.84RR46 pKa = 11.84RR47 pKa = 11.84LIFFNALLTLKK58 pKa = 10.47KK59 pKa = 10.46AFPLVTRR66 pKa = 11.84DD67 pKa = 3.0RR68 pKa = 11.84DD69 pKa = 3.8VVQRR73 pKa = 11.84ALQKK77 pKa = 10.35HH78 pKa = 5.5RR79 pKa = 11.84EE80 pKa = 4.16VLASPPSSVSSAPGITWAVKK100 pKa = 9.87RR101 pKa = 11.84MAAVRR106 pKa = 11.84PILSQEE112 pKa = 4.1VLRR115 pKa = 11.84STSCSAPGASLPTTQGITEE134 pKa = 4.13RR135 pKa = 11.84DD136 pKa = 3.14RR137 pKa = 11.84FYY139 pKa = 10.74TIHH142 pKa = 7.37KK143 pKa = 10.14DD144 pKa = 3.58VLEE147 pKa = 4.32KK148 pKa = 7.63TTKK151 pKa = 9.24VISRR155 pKa = 11.84VVYY158 pKa = 10.35EE159 pKa = 5.04DD160 pKa = 3.1IPRR163 pKa = 11.84TDD165 pKa = 2.49SRR167 pKa = 11.84PIYY170 pKa = 9.83RR171 pKa = 11.84RR172 pKa = 11.84WGNQYY177 pKa = 11.05GSGITKK183 pKa = 10.05KK184 pKa = 10.08VLRR187 pKa = 11.84NQAYY191 pKa = 7.37TVMSRR196 pKa = 11.84RR197 pKa = 11.84PVTRR201 pKa = 11.84WEE203 pKa = 3.99EE204 pKa = 4.25VEE206 pKa = 4.09VEE208 pKa = 4.0RR209 pKa = 11.84LVRR212 pKa = 11.84KK213 pKa = 6.71PTVVEE218 pKa = 4.52RR219 pKa = 11.84PADD222 pKa = 3.62VFAGSPYY229 pKa = 10.73LDD231 pKa = 3.55GLRR234 pKa = 11.84LLKK237 pKa = 10.85DD238 pKa = 3.27KK239 pKa = 10.75AQPLTWTPGVRR250 pKa = 11.84LRR252 pKa = 11.84LSLLGNFPDD261 pKa = 4.04KK262 pKa = 11.28ACMRR266 pKa = 11.84RR267 pKa = 11.84APGAGSVRR275 pKa = 11.84SQFPFDD281 pKa = 3.7RR282 pKa = 11.84SVVFSKK288 pKa = 10.34PLPIRR293 pKa = 11.84PVPGVEE299 pKa = 4.09VRR301 pKa = 11.84PTDD304 pKa = 4.96LIHH307 pKa = 7.54RR308 pKa = 11.84DD309 pKa = 3.43YY310 pKa = 11.55LSAVKK315 pKa = 10.21DD316 pKa = 3.27QLMRR320 pKa = 11.84VASAAVGEE328 pKa = 4.64TGAEE332 pKa = 4.29AIPIPDD338 pKa = 3.44KK339 pKa = 11.31GKK341 pKa = 9.99VRR343 pKa = 11.84VITKK347 pKa = 7.88EE348 pKa = 3.78HH349 pKa = 6.68ASAKK353 pKa = 9.93CLVPIQTLVSLWLSGFRR370 pKa = 11.84EE371 pKa = 4.05TSITRR376 pKa = 11.84RR377 pKa = 11.84PQTTDD382 pKa = 3.65DD383 pKa = 4.07ILWIATDD390 pKa = 4.24PDD392 pKa = 4.02ASPDD396 pKa = 3.5SCFVSGDD403 pKa = 3.66YY404 pKa = 10.95EE405 pKa = 4.31SATDD409 pKa = 4.02YY410 pKa = 11.03MPLEE414 pKa = 4.28YY415 pKa = 10.71SRR417 pKa = 11.84AFWIAIISVIPGLSDD432 pKa = 3.79LEE434 pKa = 4.13KK435 pKa = 10.69QVIIGQFYY443 pKa = 10.43ARR445 pKa = 11.84RR446 pKa = 11.84ISYY449 pKa = 9.13PKK451 pKa = 10.58GSGIPPLLQRR461 pKa = 11.84RR462 pKa = 11.84GQLMGNLLSFPALTGINLISFRR484 pKa = 11.84SLFPYY489 pKa = 10.04RR490 pKa = 11.84RR491 pKa = 11.84VLINGDD497 pKa = 4.23DD498 pKa = 3.41ILFRR502 pKa = 11.84ATKK505 pKa = 10.2PEE507 pKa = 3.51FDD509 pKa = 3.08KK510 pKa = 10.89WVEE513 pKa = 4.0HH514 pKa = 5.02VQSYY518 pKa = 10.43GLVINVRR525 pKa = 11.84KK526 pKa = 8.38TIFSPFVANINSATYY541 pKa = 8.62HH542 pKa = 5.47WVPEE546 pKa = 4.05RR547 pKa = 11.84RR548 pKa = 11.84AVIQIPRR555 pKa = 11.84YY556 pKa = 8.18PYY558 pKa = 10.08HH559 pKa = 7.12LATTLHH565 pKa = 6.6PGGNVVLDD573 pKa = 3.9CSVKK577 pKa = 9.76EE578 pKa = 3.79SSIIKK583 pKa = 9.97RR584 pKa = 11.84FYY586 pKa = 10.03PFHH589 pKa = 6.89LNRR592 pKa = 11.84RR593 pKa = 11.84DD594 pKa = 3.29TRR596 pKa = 11.84PFFGEE601 pKa = 3.77TWRR604 pKa = 11.84GGMGFTYY611 pKa = 10.63AGFRR615 pKa = 11.84PSKK618 pKa = 8.75QEE620 pKa = 2.9RR621 pKa = 11.84WLYY624 pKa = 10.55LRR626 pKa = 11.84FRR628 pKa = 11.84AKK630 pKa = 10.42YY631 pKa = 9.32GRR633 pKa = 11.84KK634 pKa = 8.56PPCAIPRR641 pKa = 11.84HH642 pKa = 5.47LLQEE646 pKa = 4.08PSSIDD651 pKa = 3.13RR652 pKa = 11.84VLVRR656 pKa = 11.84TLGPYY661 pKa = 10.22SDD663 pKa = 6.01DD664 pKa = 4.05SICRR668 pKa = 11.84TGSSVSRR675 pKa = 11.84PTFRR679 pKa = 11.84IPEE682 pKa = 4.15KK683 pKa = 10.64LKK685 pKa = 10.73SLPQEE690 pKa = 3.93KK691 pKa = 10.23VKK693 pKa = 10.73EE694 pKa = 4.01ISQGKK699 pKa = 9.94LIFPSLGKK707 pKa = 9.64IDD709 pKa = 4.48CGLAGSTGTLPIGG722 pKa = 3.7

Molecular weight: 81.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KIH2|A0A1L3KIH2_9VIRU RNA-dependent RNA polymerase OS=Hubei narna-like virus 13 OX=1922943 PE=4 SV=1
MM1 pKa = 7.7AISTPVTRR9 pKa = 11.84GGLRR13 pKa = 11.84KK14 pKa = 9.43QLEE17 pKa = 4.05GLARR21 pKa = 11.84GTGFLTRR28 pKa = 11.84SGCVVFAARR37 pKa = 11.84LASARR42 pKa = 11.84SFRR45 pKa = 11.84RR46 pKa = 11.84RR47 pKa = 11.84LIFFNALLTLKK58 pKa = 10.47KK59 pKa = 10.46AFPLVTRR66 pKa = 11.84DD67 pKa = 3.0RR68 pKa = 11.84DD69 pKa = 3.8VVQRR73 pKa = 11.84ALQKK77 pKa = 10.35HH78 pKa = 5.5RR79 pKa = 11.84EE80 pKa = 4.16VLASPPSSVSSAPGITWAVKK100 pKa = 9.87RR101 pKa = 11.84MAAVRR106 pKa = 11.84PILSQEE112 pKa = 4.1VLRR115 pKa = 11.84STSCSAPGASLPTTQGITEE134 pKa = 4.13RR135 pKa = 11.84DD136 pKa = 3.14RR137 pKa = 11.84FYY139 pKa = 10.74TIHH142 pKa = 7.37KK143 pKa = 10.14DD144 pKa = 3.58VLEE147 pKa = 4.32KK148 pKa = 7.63TTKK151 pKa = 9.24VISRR155 pKa = 11.84VVYY158 pKa = 10.35EE159 pKa = 5.04DD160 pKa = 3.1IPRR163 pKa = 11.84TDD165 pKa = 2.49SRR167 pKa = 11.84PIYY170 pKa = 9.83RR171 pKa = 11.84RR172 pKa = 11.84WGNQYY177 pKa = 11.05GSGITKK183 pKa = 10.05KK184 pKa = 10.08VLRR187 pKa = 11.84NQAYY191 pKa = 7.37TVMSRR196 pKa = 11.84RR197 pKa = 11.84PVTRR201 pKa = 11.84WEE203 pKa = 3.99EE204 pKa = 4.25VEE206 pKa = 4.09VEE208 pKa = 4.0RR209 pKa = 11.84LVRR212 pKa = 11.84KK213 pKa = 6.71PTVVEE218 pKa = 4.52RR219 pKa = 11.84PADD222 pKa = 3.62VFAGSPYY229 pKa = 10.73LDD231 pKa = 3.55GLRR234 pKa = 11.84LLKK237 pKa = 10.85DD238 pKa = 3.27KK239 pKa = 10.75AQPLTWTPGVRR250 pKa = 11.84LRR252 pKa = 11.84LSLLGNFPDD261 pKa = 4.04KK262 pKa = 11.28ACMRR266 pKa = 11.84RR267 pKa = 11.84APGAGSVRR275 pKa = 11.84SQFPFDD281 pKa = 3.7RR282 pKa = 11.84SVVFSKK288 pKa = 10.34PLPIRR293 pKa = 11.84PVPGVEE299 pKa = 4.09VRR301 pKa = 11.84PTDD304 pKa = 4.96LIHH307 pKa = 7.54RR308 pKa = 11.84DD309 pKa = 3.43YY310 pKa = 11.55LSAVKK315 pKa = 10.21DD316 pKa = 3.27QLMRR320 pKa = 11.84VASAAVGEE328 pKa = 4.64TGAEE332 pKa = 4.29AIPIPDD338 pKa = 3.44KK339 pKa = 11.31GKK341 pKa = 9.99VRR343 pKa = 11.84VITKK347 pKa = 7.88EE348 pKa = 3.78HH349 pKa = 6.68ASAKK353 pKa = 9.93CLVPIQTLVSLWLSGFRR370 pKa = 11.84EE371 pKa = 4.05TSITRR376 pKa = 11.84RR377 pKa = 11.84PQTTDD382 pKa = 3.65DD383 pKa = 4.07ILWIATDD390 pKa = 4.24PDD392 pKa = 4.02ASPDD396 pKa = 3.5SCFVSGDD403 pKa = 3.66YY404 pKa = 10.95EE405 pKa = 4.31SATDD409 pKa = 4.02YY410 pKa = 11.03MPLEE414 pKa = 4.28YY415 pKa = 10.71SRR417 pKa = 11.84AFWIAIISVIPGLSDD432 pKa = 3.79LEE434 pKa = 4.13KK435 pKa = 10.69QVIIGQFYY443 pKa = 10.43ARR445 pKa = 11.84RR446 pKa = 11.84ISYY449 pKa = 9.13PKK451 pKa = 10.58GSGIPPLLQRR461 pKa = 11.84RR462 pKa = 11.84GQLMGNLLSFPALTGINLISFRR484 pKa = 11.84SLFPYY489 pKa = 10.04RR490 pKa = 11.84RR491 pKa = 11.84VLINGDD497 pKa = 4.23DD498 pKa = 3.41ILFRR502 pKa = 11.84ATKK505 pKa = 10.2PEE507 pKa = 3.51FDD509 pKa = 3.08KK510 pKa = 10.89WVEE513 pKa = 4.0HH514 pKa = 5.02VQSYY518 pKa = 10.43GLVINVRR525 pKa = 11.84KK526 pKa = 8.38TIFSPFVANINSATYY541 pKa = 8.62HH542 pKa = 5.47WVPEE546 pKa = 4.05RR547 pKa = 11.84RR548 pKa = 11.84AVIQIPRR555 pKa = 11.84YY556 pKa = 8.18PYY558 pKa = 10.08HH559 pKa = 7.12LATTLHH565 pKa = 6.6PGGNVVLDD573 pKa = 3.9CSVKK577 pKa = 9.76EE578 pKa = 3.79SSIIKK583 pKa = 9.97RR584 pKa = 11.84FYY586 pKa = 10.03PFHH589 pKa = 6.89LNRR592 pKa = 11.84RR593 pKa = 11.84DD594 pKa = 3.29TRR596 pKa = 11.84PFFGEE601 pKa = 3.77TWRR604 pKa = 11.84GGMGFTYY611 pKa = 10.63AGFRR615 pKa = 11.84PSKK618 pKa = 8.75QEE620 pKa = 2.9RR621 pKa = 11.84WLYY624 pKa = 10.55LRR626 pKa = 11.84FRR628 pKa = 11.84AKK630 pKa = 10.42YY631 pKa = 9.32GRR633 pKa = 11.84KK634 pKa = 8.56PPCAIPRR641 pKa = 11.84HH642 pKa = 5.47LLQEE646 pKa = 4.08PSSIDD651 pKa = 3.13RR652 pKa = 11.84VLVRR656 pKa = 11.84TLGPYY661 pKa = 10.22SDD663 pKa = 6.01DD664 pKa = 4.05SICRR668 pKa = 11.84TGSSVSRR675 pKa = 11.84PTFRR679 pKa = 11.84IPEE682 pKa = 4.15KK683 pKa = 10.64LKK685 pKa = 10.73SLPQEE690 pKa = 3.93KK691 pKa = 10.23VKK693 pKa = 10.73EE694 pKa = 4.01ISQGKK699 pKa = 9.94LIFPSLGKK707 pKa = 9.64IDD709 pKa = 4.48CGLAGSTGTLPIGG722 pKa = 3.7

Molecular weight: 81.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1

0

1

722

722

722

722.0

81.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.51 ± 0.0

1.247 ± 0.0

4.432 ± 0.0

4.017 ± 0.0

4.432 ± 0.0

6.787 ± 0.0

1.385 ± 0.0

6.371 ± 0.0

4.986 ± 0.0

9.141 ± 0.0

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.108 ± 0.0

1.662 ± 0.0

7.756 ± 0.0

3.047 ± 0.0

10.249 ± 0.0

8.31 ± 0.0

6.233 ± 0.0

7.756 ± 0.0

1.524 ± 0.0

3.047 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski