Acinetobacter phage AbP2
Average proteome isoelectric point is 6.3
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 88 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A220NQH6|A0A220NQH6_9CAUD Putative tail fiber protein OS=Acinetobacter phage AbP2 OX=2015804 GN=ABP2_030 PE=4 SV=1
MM1 pKa = 7.9 IYY3 pKa = 9.92 QIPLAQVPNQFFTTSLNGVTWAITLEE29 pKa = 4.08 TRR31 pKa = 11.84 LNNLYY36 pKa = 10.27 ISLSNNNDD44 pKa = 2.72 GDD46 pKa = 3.93 VLLNRR51 pKa = 11.84 ICLNRR56 pKa = 11.84 TYY58 pKa = 10.23 LGHH61 pKa = 6.75 GFIFVDD67 pKa = 3.51 IDD69 pKa = 3.79 GNSDD73 pKa = 3.53 PEE75 pKa = 4.25 YY76 pKa = 9.62 TGLGTRR82 pKa = 11.84 YY83 pKa = 10.14 LLIWTDD89 pKa = 3.71 EE90 pKa = 4.13 VV91 pKa = 3.51
Molecular weight: 10.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.019
IPC2_protein 4.317
IPC_protein 4.151
Toseland 3.961
ProMoST 4.329
Dawson 4.151
Bjellqvist 4.304
Wikipedia 4.101
Rodwell 3.999
Grimsley 3.884
Solomon 4.139
Lehninger 4.088
Nozaki 4.279
DTASelect 4.507
Thurlkill 4.024
EMBOSS 4.113
Sillero 4.279
Patrickios 0.782
IPC_peptide 4.126
IPC2_peptide 4.253
IPC2.peptide.svr19 4.167
Protein with the highest isoelectric point:
>tr|A0A220NQP3|A0A220NQP3_9CAUD Putative DNA-binding protein OS=Acinetobacter phage AbP2 OX=2015804 GN=ABP2_085 PE=4 SV=1
MM1 pKa = 7.62 SKK3 pKa = 10.18 PATSFLFLGEE13 pKa = 4.39 LKK15 pKa = 10.68 LLEE18 pKa = 4.07 STIIEE23 pKa = 4.01 NTKK26 pKa = 8.23 RR27 pKa = 11.84 TKK29 pKa = 10.5 VRR31 pKa = 11.84 DD32 pKa = 3.78 AIRR35 pKa = 11.84 RR36 pKa = 11.84 YY37 pKa = 10.37 GDD39 pKa = 3.17 RR40 pKa = 11.84 KK41 pKa = 10.6 FNTTQVGNNVIVTRR55 pKa = 11.84 IQQ57 pKa = 2.88
Molecular weight: 6.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.277
IPC2_protein 9.677
IPC_protein 10.262
Toseland 10.877
ProMoST 10.482
Dawson 10.935
Bjellqvist 10.584
Wikipedia 11.111
Rodwell 11.272
Grimsley 10.965
Solomon 11.067
Lehninger 11.038
Nozaki 10.833
DTASelect 10.599
Thurlkill 10.847
EMBOSS 11.272
Sillero 10.862
Patrickios 11.052
IPC_peptide 11.067
IPC2_peptide 9.151
IPC2.peptide.svr19 8.762
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
88
0
88
13983
32
776
158.9
17.91
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.223 ± 0.338
1.223 ± 0.127
6.05 ± 0.249
6.708 ± 0.404
4.491 ± 0.233
6.851 ± 0.283
1.666 ± 0.141
7.23 ± 0.223
7.638 ± 0.33
8.017 ± 0.273
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.853 ± 0.168
5.993 ± 0.294
2.904 ± 0.146
3.826 ± 0.258
3.876 ± 0.185
6.301 ± 0.201
5.364 ± 0.364
6.436 ± 0.268
1.416 ± 0.1
3.933 ± 0.187
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here