Marinobacter sp. Z-F4-2
Average proteome isoelectric point is 6.16
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2568 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2T4DBT8|A0A2T4DBT8_9ALTE Fatty acid-binding protein DegV (Fragment) OS=Marinobacter sp. Z-F4-2 OX=2137199 GN=C9993_12540 PE=4 SV=1
MM1 pKa = 7.74 SDD3 pKa = 3.39 FRR5 pKa = 11.84 EE6 pKa = 4.16 EE7 pKa = 5.22 LEE9 pKa = 4.34 GCQGNKK15 pKa = 10.18 SLILGNGFGISYY27 pKa = 10.45 DD28 pKa = 3.35 VAARR32 pKa = 11.84 TDD34 pKa = 3.76 SFCWDD39 pKa = 3.79 SLADD43 pKa = 4.67 LCDD46 pKa = 4.09 FDD48 pKa = 5.34 NDD50 pKa = 3.71 NPLLEE55 pKa = 5.0 LLEE58 pKa = 4.24 EE59 pKa = 4.62 CNFDD63 pKa = 4.91 FEE65 pKa = 5.41 IVHH68 pKa = 5.8 QKK70 pKa = 9.83 INNAISVII78 pKa = 3.82
Molecular weight: 8.78 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.752
IPC2_protein 3.821
IPC_protein 3.745
Toseland 3.554
ProMoST 3.923
Dawson 3.745
Bjellqvist 3.91
Wikipedia 3.694
Rodwell 3.579
Grimsley 3.465
Solomon 3.719
Lehninger 3.681
Nozaki 3.872
DTASelect 4.075
Thurlkill 3.617
EMBOSS 3.694
Sillero 3.872
Patrickios 1.875
IPC_peptide 3.719
IPC2_peptide 3.846
IPC2.peptide.svr19 3.796
Protein with the highest isoelectric point:
>tr|A0A2T4E0F0|A0A2T4E0F0_9ALTE Acyl-coenzyme A dehydrogenase OS=Marinobacter sp. Z-F4-2 OX=2137199 GN=C9993_02600 PE=3 SV=1
MM1 pKa = 7.24 SRR3 pKa = 11.84 RR4 pKa = 11.84 RR5 pKa = 11.84 KK6 pKa = 9.74 YY7 pKa = 9.74 SPEE10 pKa = 3.78 FKK12 pKa = 10.41 RR13 pKa = 11.84 EE14 pKa = 3.99 AIALTRR20 pKa = 11.84 QPGVSCRR27 pKa = 11.84 QVALEE32 pKa = 4.24 IGINPNLLSRR42 pKa = 11.84 WRR44 pKa = 11.84 RR45 pKa = 11.84 EE46 pKa = 3.64 ADD48 pKa = 3.35 EE49 pKa = 4.4 EE50 pKa = 4.06 TDD52 pKa = 3.55 KK53 pKa = 11.54 AFKK56 pKa = 10.81 GSGSPRR62 pKa = 11.84 DD63 pKa = 3.6 EE64 pKa = 3.44 EE65 pKa = 4.31 VARR68 pKa = 11.84 LKK70 pKa = 10.99 RR71 pKa = 11.84 EE72 pKa = 3.69 LARR75 pKa = 11.84 VKK77 pKa = 10.55 KK78 pKa = 10.29 EE79 pKa = 3.49 RR80 pKa = 11.84 GFFARR85 pKa = 11.84 SGSVLRR91 pKa = 11.84 KK92 pKa = 9.88 GVVLRR97 pKa = 11.84 YY98 pKa = 9.18 QAIQRR103 pKa = 11.84 CRR105 pKa = 11.84 NTFPIRR111 pKa = 11.84 LMCRR115 pKa = 11.84 CLKK118 pKa = 10.57 VSASGYY124 pKa = 7.0 YY125 pKa = 9.83 AWVSRR130 pKa = 11.84 PEE132 pKa = 4.12 SARR135 pKa = 11.84 SKK137 pKa = 11.05 ANQHH141 pKa = 5.5 LLSRR145 pKa = 11.84 IRR147 pKa = 11.84 EE148 pKa = 3.94 IHH150 pKa = 7.05 DD151 pKa = 4.18 DD152 pKa = 3.73 SGGMIGSPRR161 pKa = 11.84 MHH163 pKa = 7.24 EE164 pKa = 4.12 DD165 pKa = 3.22 LVAEE169 pKa = 4.6 GASTSLNRR177 pKa = 11.84 VARR180 pKa = 11.84 LMAKK184 pKa = 10.01 HH185 pKa = 6.92 GIQGWPRR192 pKa = 11.84 KK193 pKa = 9.58 KK194 pKa = 10.05 RR195 pKa = 11.84 GRR197 pKa = 11.84 LGRR200 pKa = 11.84 QSYY203 pKa = 9.62 RR204 pKa = 11.84 PPGIRR209 pKa = 11.84 NHH211 pKa = 6.25 LEE213 pKa = 3.62 RR214 pKa = 11.84 DD215 pKa = 4.45 FSALEE220 pKa = 4.44 PDD222 pKa = 4.25 TKK224 pKa = 10.22 WVTDD228 pKa = 3.18 ITEE231 pKa = 4.07 IPTQEE236 pKa = 3.94 GKK238 pKa = 10.91 LFLCVVLDD246 pKa = 4.41 LFSKK250 pKa = 10.8 LIVGWSMHH258 pKa = 5.4 HH259 pKa = 5.74 RR260 pKa = 11.84 QDD262 pKa = 2.6 RR263 pKa = 11.84 HH264 pKa = 3.58 MVIRR268 pKa = 11.84 AVEE271 pKa = 3.81 MAVWQRR277 pKa = 11.84 QGKK280 pKa = 9.2 VPVILHH286 pKa = 6.05 SDD288 pKa = 3.38 RR289 pKa = 11.84 GSQFTSGDD297 pKa = 3.41 YY298 pKa = 10.36 QRR300 pKa = 11.84 YY301 pKa = 8.95 LGSNRR306 pKa = 11.84 LVSSMSAVGHH316 pKa = 6.43 CGDD319 pKa = 3.77 NAACEE324 pKa = 4.52 GFFGMLKK331 pKa = 9.98 RR332 pKa = 11.84 EE333 pKa = 4.44 RR334 pKa = 11.84 IHH336 pKa = 5.73 HH337 pKa = 5.51 RR338 pKa = 11.84 RR339 pKa = 11.84 YY340 pKa = 8.89 RR341 pKa = 11.84 TQNEE345 pKa = 3.11 ARR347 pKa = 11.84 ADD349 pKa = 3.49 VFDD352 pKa = 4.63 YY353 pKa = 10.82 IEE355 pKa = 4.7 RR356 pKa = 11.84 FHH358 pKa = 6.55 NPRR361 pKa = 11.84 MRR363 pKa = 11.84 RR364 pKa = 11.84 RR365 pKa = 11.84 VAAQDD370 pKa = 3.12 RR371 pKa = 11.84 KK372 pKa = 10.57 LFAVLKK378 pKa = 9.91 PSVEE382 pKa = 4.31 TGG384 pKa = 2.95
Molecular weight: 44.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.349
IPC2_protein 9.633
IPC_protein 10.555
Toseland 10.774
ProMoST 10.54
Dawson 10.847
Bjellqvist 10.599
Wikipedia 11.096
Rodwell 10.979
Grimsley 10.891
Solomon 11.008
Lehninger 10.979
Nozaki 10.76
DTASelect 10.584
Thurlkill 10.774
EMBOSS 11.184
Sillero 10.789
Patrickios 10.672
IPC_peptide 11.023
IPC2_peptide 9.721
IPC2.peptide.svr19 8.678
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2568
0
2568
642497
20
1734
250.2
27.75
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.519 ± 0.062
0.96 ± 0.016
5.781 ± 0.037
6.833 ± 0.052
3.945 ± 0.035
7.523 ± 0.05
2.197 ± 0.029
5.298 ± 0.037
4.082 ± 0.055
10.558 ± 0.059
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.475 ± 0.029
3.395 ± 0.033
4.511 ± 0.034
4.017 ± 0.043
6.477 ± 0.047
6.156 ± 0.039
5.064 ± 0.028
7.081 ± 0.047
1.427 ± 0.02
2.7 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here