Halonotius sp. F13-13
Average proteome isoelectric point is 4.75
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2460 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3A6PSN4|A0A3A6PSN4_9EURY Uncharacterized protein OS=Halonotius sp. F13-13 OX=2216978 GN=DM826_12615 PE=4 SV=1
MM1 pKa = 7.46 TISDD5 pKa = 3.67 RR6 pKa = 11.84 VVFDD10 pKa = 4.21 AEE12 pKa = 4.24 PLVAHH17 pKa = 7.24 ADD19 pKa = 3.81 DD20 pKa = 4.57 EE21 pKa = 5.0 PGSEE25 pKa = 4.22 VVEE28 pKa = 5.02 DD29 pKa = 3.94 YY30 pKa = 11.47 LDD32 pKa = 3.96 AVAADD37 pKa = 3.91 DD38 pKa = 3.71 TTGYY42 pKa = 10.45 VSYY45 pKa = 9.83 VTLTEE50 pKa = 3.51 VRR52 pKa = 11.84 YY53 pKa = 9.89 ILSQKK58 pKa = 9.24 YY59 pKa = 10.12 DD60 pKa = 3.1 RR61 pKa = 11.84 GTADD65 pKa = 5.12 EE66 pKa = 4.7 YY67 pKa = 11.07 IDD69 pKa = 3.58 WLTDD73 pKa = 3.49 LGIEE77 pKa = 4.14 PVGVADD83 pKa = 4.54 CWQGTADD90 pKa = 4.44 HH91 pKa = 6.37 VLSHH95 pKa = 6.42 NPALGDD101 pKa = 3.66 AFALATADD109 pKa = 4.28 HH110 pKa = 7.21 LDD112 pKa = 3.28 ATLLVGADD120 pKa = 3.96 DD121 pKa = 6.2 DD122 pKa = 4.64 YY123 pKa = 12.06 DD124 pKa = 4.16 AVDD127 pKa = 4.23 DD128 pKa = 4.23 VSITRR133 pKa = 11.84 FRR135 pKa = 11.84 NEE137 pKa = 3.42 AGG139 pKa = 3.28
Molecular weight: 15.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.69
IPC2_protein 3.643
IPC_protein 3.668
Toseland 3.439
ProMoST 3.859
Dawson 3.681
Bjellqvist 3.834
Wikipedia 3.656
Rodwell 3.49
Grimsley 3.35
Solomon 3.668
Lehninger 3.63
Nozaki 3.795
DTASelect 4.088
Thurlkill 3.503
EMBOSS 3.656
Sillero 3.795
Patrickios 1.036
IPC_peptide 3.656
IPC2_peptide 3.77
IPC2.peptide.svr19 3.73
Protein with the highest isoelectric point:
>tr|A0A3A6PUN0|A0A3A6PUN0_9EURY Uncharacterized protein OS=Halonotius sp. F13-13 OX=2216978 GN=DM826_10195 PE=4 SV=1
MM1 pKa = 7.22 VVGLVVTAFWAMLPAYY17 pKa = 9.57 VPNNAAVLAGGGRR30 pKa = 11.84 PIDD33 pKa = 4.31 GGRR36 pKa = 11.84 TWGDD40 pKa = 2.84 RR41 pKa = 11.84 RR42 pKa = 11.84 LLGDD46 pKa = 3.85 GKK48 pKa = 7.53 TWRR51 pKa = 11.84 GTAVGTLVGITLAAGLNVAAAPLGSAIGFSLPTFPPAGALGLAAGAMAGDD101 pKa = 4.12 IAASFVKK108 pKa = 10.14 RR109 pKa = 11.84 RR110 pKa = 11.84 SGRR113 pKa = 11.84 ARR115 pKa = 11.84 GAAFPGLDD123 pKa = 3.01 QLDD126 pKa = 4.07 FVVGAILFAFVLAPGWAQATFTLGVLAVIVVVTPVLHH163 pKa = 6.23 VGTNIIAYY171 pKa = 10.09 LIGAKK176 pKa = 10.04 NEE178 pKa = 3.87 PWW180 pKa = 3.37
Molecular weight: 18.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.324
IPC2_protein 9.633
IPC_protein 10.555
Toseland 10.628
ProMoST 10.379
Dawson 10.745
Bjellqvist 10.467
Wikipedia 10.979
Rodwell 10.862
Grimsley 10.804
Solomon 10.877
Lehninger 10.847
Nozaki 10.599
DTASelect 10.467
Thurlkill 10.643
EMBOSS 11.038
Sillero 10.672
Patrickios 10.672
IPC_peptide 10.891
IPC2_peptide 9.311
IPC2.peptide.svr19 8.669
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2460
0
2460
717023
10
1850
291.5
31.58
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.184 ± 0.077
0.7 ± 0.015
8.847 ± 0.07
8.205 ± 0.07
3.177 ± 0.033
8.082 ± 0.053
1.956 ± 0.025
4.901 ± 0.043
1.937 ± 0.033
8.84 ± 0.052
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.703 ± 0.022
2.472 ± 0.028
4.611 ± 0.033
2.921 ± 0.03
5.834 ± 0.039
5.549 ± 0.036
7.094 ± 0.042
8.352 ± 0.054
1.041 ± 0.018
2.595 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here