Streptococcus phage Javan37
Average proteome isoelectric point is 6.09
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 57 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D6A9J0|A0A4D6A9J0_9CAUD DUF3799 domain-containing protein OS=Streptococcus phage Javan37 OX=2548130 GN=Javan37_0038 PE=4 SV=1
MM1 pKa = 7.89 EE2 pKa = 5.85 LLQYY6 pKa = 10.68 NNKK9 pKa = 9.88 KK10 pKa = 10.05 ISLVDD15 pKa = 3.22 IDD17 pKa = 4.66 DD18 pKa = 5.14 EE19 pKa = 4.43 IWTGTAYY26 pKa = 11.08 YY27 pKa = 10.52 CDD29 pKa = 4.2 ADD31 pKa = 4.04 TNEE34 pKa = 4.01 TSEE37 pKa = 4.59 DD38 pKa = 3.59 VLVVKK43 pKa = 10.1 NEE45 pKa = 3.8 RR46 pKa = 11.84 GYY48 pKa = 9.13 TEE50 pKa = 3.79 ISEE53 pKa = 4.55 SEE55 pKa = 3.94 IKK57 pKa = 10.61 SIEE60 pKa = 4.11 III62 pKa = 3.76
Molecular weight: 7.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.061
IPC2_protein 3.999
IPC_protein 3.884
Toseland 3.706
ProMoST 4.024
Dawson 3.846
Bjellqvist 4.012
Wikipedia 3.757
Rodwell 3.719
Grimsley 3.63
Solomon 3.834
Lehninger 3.783
Nozaki 3.973
DTASelect 4.113
Thurlkill 3.757
EMBOSS 3.77
Sillero 3.999
Patrickios 3.02
IPC_peptide 3.834
IPC2_peptide 3.973
IPC2.peptide.svr19 3.902
Protein with the highest isoelectric point:
>tr|A0A4D6ABF1|A0A4D6ABF1_9CAUD Uncharacterized protein OS=Streptococcus phage Javan37 OX=2548130 GN=Javan37_0026 PE=4 SV=1
MM1 pKa = 7.53 SKK3 pKa = 10.69 FKK5 pKa = 10.97 FKK7 pKa = 10.93 LNKK10 pKa = 10.25 AGVAEE15 pKa = 4.25 LMKK18 pKa = 10.49 SSEE21 pKa = 4.17 MQQVLTTKK29 pKa = 9.48 ATAIRR34 pKa = 11.84 EE35 pKa = 4.24 RR36 pKa = 11.84 CGDD39 pKa = 3.93 GYY41 pKa = 11.45 AQDD44 pKa = 2.92 IHH46 pKa = 7.92 VGKK49 pKa = 10.15 NRR51 pKa = 11.84 ANAMVSAKK59 pKa = 9.54 TIKK62 pKa = 10.38 AKK64 pKa = 10.43 KK65 pKa = 10.15 DD66 pKa = 3.23 NSKK69 pKa = 11.36 NNTLLKK75 pKa = 10.41 AVRR78 pKa = 3.79
Molecular weight: 8.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.11
IPC2_protein 9.502
IPC_protein 9.443
Toseland 10.643
ProMoST 10.189
Dawson 10.701
Bjellqvist 10.248
Wikipedia 10.774
Rodwell 11.447
Grimsley 10.716
Solomon 10.73
Lehninger 10.73
Nozaki 10.599
DTASelect 10.248
Thurlkill 10.599
EMBOSS 11.008
Sillero 10.613
Patrickios 11.199
IPC_peptide 10.745
IPC2_peptide 8.653
IPC2.peptide.svr19 8.478
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
57
0
57
10842
38
1271
190.2
21.55
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.244 ± 0.383
0.738 ± 0.106
6.41 ± 0.27
7.323 ± 0.444
4.04 ± 0.258
6.549 ± 0.338
1.513 ± 0.167
7.572 ± 0.282
8.744 ± 0.401
8.19 ± 0.321
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.5 ± 0.204
5.875 ± 0.254
2.73 ± 0.201
3.791 ± 0.193
3.883 ± 0.238
6.595 ± 0.355
6.041 ± 0.391
5.774 ± 0.268
1.264 ± 0.134
4.224 ± 0.299
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here