Mus musculus (Mouse)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria;

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 63656 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B0GSR9|A0A1B0GSR9_MOUSE Isoform of P06151 L-lactate dehydrogenase OS=Mus musculus OX=10090 GN=Ldha PE=1 SV=1
MM1 pKa = 7.1EE2 pKa = 3.86QAADD6 pKa = 4.36LFNEE10 pKa = 4.26DD11 pKa = 3.11WDD13 pKa = 4.81LEE15 pKa = 4.38LKK17 pKa = 10.49ADD19 pKa = 3.94QGNPYY24 pKa = 10.37DD25 pKa = 4.89ADD27 pKa = 5.08DD28 pKa = 3.93IQGSISQEE36 pKa = 3.42IKK38 pKa = 10.32PWVCCAPQGDD48 pKa = 4.66MIYY51 pKa = 10.59DD52 pKa = 4.29PSWHH56 pKa = 6.9HH57 pKa = 6.75PPPLIPHH64 pKa = 5.6YY65 pKa = 10.76SKK67 pKa = 10.49MVFEE71 pKa = 5.05TGQFDD76 pKa = 4.21DD77 pKa = 6.01AEE79 pKa = 4.35DD80 pKa = 3.31

Molecular weight: 9.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G3X9A2|G3X9A2_MOUSE Keratin-associated protein 24-1 OS=Mus musculus OX=10090 GN=Krtap24-1 PE=4 SV=1
MM1 pKa = 7.72RR2 pKa = 11.84SSGKK6 pKa = 10.53APMLQRR12 pKa = 11.84SPASPAASFSMAWALPGVRR31 pKa = 11.84RR32 pKa = 11.84PGTPTASPAQLGLRR46 pKa = 11.84AHH48 pKa = 6.65HH49 pKa = 6.69LLLGRR54 pKa = 11.84SGAPRR59 pKa = 11.84LAAQLSLGSHH69 pKa = 5.66VASLLPRR76 pKa = 11.84GISPHH81 pKa = 5.18PHH83 pKa = 6.52PRR85 pKa = 11.84PAPRR89 pKa = 11.84PPGPHH94 pKa = 6.65PRR96 pKa = 11.84SAQASFLRR104 pKa = 11.84KK105 pKa = 9.75KK106 pKa = 10.6INN108 pKa = 3.07

Molecular weight: 11.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

22001

41655

63656

28559426

5

35390

448.7

50.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.85 ± 0.012

2.221 ± 0.01

4.853 ± 0.009

7.086 ± 0.017

3.627 ± 0.009

6.332 ± 0.014

2.598 ± 0.007

4.353 ± 0.011

5.724 ± 0.017

9.947 ± 0.02

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.222 ± 0.006

3.58 ± 0.008

6.2 ± 0.016

4.848 ± 0.014

5.616 ± 0.011

8.586 ± 0.017

5.443 ± 0.014

6.066 ± 0.013

1.187 ± 0.004

2.645 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski