Proteome-pI 2.0 - Proteome Isoelectric Point Database

Methylobacterium radiotolerans (strain ATCC 27329 / DSM 1819 / JCM 2831 / NBRC 15690 / NCIMB 10815 / 0-1)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Methylobacteriaceae; Methylobacterium; Methylobacterium radiotolerans

Average proteome isoelectric point is 6.95

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 6388 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions

Protein with the lowest isoelectric point:

>tr|B1M9M7|B1M9M7_METRJ Uncharacterized protein OS=Methylobacterium radiotolerans (strain ATCC 27329 / DSM 1819 / JCM 2831 / NBRC 15690 / NCIMB 10815 / 0-1) OX=426355 GN=Mrad2831_6279 PE=4 SV=1

MM1 pKa = 7.37TYY3 pKa = 10.71VVTEE7 pKa = 3.51NCIRR11 pKa = 11.84CKK13 pKa = 10.23YY14 pKa = 7.47TDD16 pKa = 3.93CVEE19 pKa = 4.15VCPVDD24 pKa = 3.47CFYY27 pKa = 11.45VGEE30 pKa = 4.3TMLVIDD36 pKa = 4.99PDD38 pKa = 3.65EE39 pKa = 5.72CIDD42 pKa = 4.45CGVCEE47 pKa = 4.63PEE49 pKa = 5.02CPADD53 pKa = 4.46AIKK56 pKa = 10.95ADD58 pKa = 4.12TEE60 pKa = 4.4PGLEE64 pKa = 3.78GWKK67 pKa = 10.61AFNAKK72 pKa = 9.2YY73 pKa = 10.09AALWPNIAEE82 pKa = 4.51KK83 pKa = 10.73VDD85 pKa = 3.83PAADD89 pKa = 3.33AAEE92 pKa = 3.93WDD94 pKa = 3.83GRR96 pKa = 11.84DD97 pKa = 3.38GKK99 pKa = 11.02LIAVFGAADD108 pKa = 3.36PAAAA112 pKa = 4.67

Molecular weight: 12.04 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>tr|B1LUS6|B1LUS6_METRJ Histidine kinase OS=Methylobacterium radiotolerans (strain ATCC 27329 / DSM 1819 / JCM 2831 / NBRC 15690 / NCIMB 10815 / 0-1) OX=426355 GN=Mrad2831_3535 PE=4 SV=1

MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATAGGRR28 pKa = 11.84KK29 pKa = 9.12VIARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AHH37 pKa = 4.95GRR39 pKa = 11.84KK40 pKa = 9.25RR41 pKa = 11.84LSAA44 pKa = 4.01

Molecular weight: 5.17 kDa
Isoelectric point according different methods:

Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
6388	0	6388	1944682	29	2487	304.4	32.6

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu
14.819 ± 0.056	0.84 ± 0.01	5.616 ± 0.024	5.267 ± 0.031	3.294 ± 0.021	9.118 ± 0.033	1.917 ± 0.014	4.279 ± 0.02	2.315 ± 0.024	10.467 ± 0.04

Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
2.013 ± 0.015	2.021 ± 0.018	5.93 ± 0.029	2.753 ± 0.017	8.371 ± 0.034	4.743 ± 0.023	5.336 ± 0.02	7.623 ± 0.026	1.243 ± 0.012	2.034 ± 0.018

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski