Cowpea mosaic virus (strain SB) (CPMV)
Average proteome isoelectric point is 7.0
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q66169|Q66169_CPMVS Uncharacterized protein (Fragment) OS=Cowpea mosaic virus (strain SB) OX=928299 PE=4 SV=2
MM1 pKa = 7.66 GLPEE5 pKa = 4.18 YY6 pKa = 10.24 EE7 pKa = 4.56 ADD9 pKa = 4.29 SEE11 pKa = 4.53 ALLSQLTIEE20 pKa = 4.8 FTPGMTVSSLLAQVTTNDD38 pKa = 3.01 FHH40 pKa = 8.82 SAIEE44 pKa = 3.83 FFAAEE49 pKa = 4.18 KK50 pKa = 10.62 AVDD53 pKa = 3.49 IEE55 pKa = 4.42 GVHH58 pKa = 5.93 YY59 pKa = 9.84 NAYY62 pKa = 7.87 MQQIRR67 pKa = 11.84 KK68 pKa = 9.04 NPSLLRR74 pKa = 11.84 ISVVAYY80 pKa = 9.61 AFHH83 pKa = 6.77 VSDD86 pKa = 4.08 MVAEE90 pKa = 4.23 TMSYY94 pKa = 10.92 DD95 pKa = 3.5 VYY97 pKa = 11.06 EE98 pKa = 4.41 FLYY101 pKa = 10.34 KK102 pKa = 10.31 HH103 pKa = 5.77 YY104 pKa = 11.29 ALFISNLVTRR114 pKa = 11.84 TLRR117 pKa = 11.84 FKK119 pKa = 10.86 EE120 pKa = 3.86 LLLFCKK126 pKa = 10.13 QQFLEE131 pKa = 4.61 KK132 pKa = 8.82 MQASIVWAPEE142 pKa = 3.65 LEE144 pKa = 4.26 QYY146 pKa = 10.8 LQVEE150 pKa = 4.95 GDD152 pKa = 3.51 AVAQGVSQLLYY163 pKa = 11.41 KK164 pKa = 9.91 MVTWVPTFVRR174 pKa = 11.84 GAVDD178 pKa = 3.33 WSVDD182 pKa = 3.58 AILVSFRR189 pKa = 11.84 KK190 pKa = 9.79 HH191 pKa = 4.73 FEE193 pKa = 4.01 KK194 pKa = 10.19 MVQEE198 pKa = 4.56 YY199 pKa = 10.17 VPMAHH204 pKa = 7.11 RR205 pKa = 11.84 VCSWLSQLWDD215 pKa = 5.13 KK216 pKa = 10.67 IVQWISQASEE226 pKa = 3.81 TMGWFLDD233 pKa = 3.84 GCRR236 pKa = 11.84 DD237 pKa = 3.5 LMTWGIATLATCSALSLVEE256 pKa = 4.05 KK257 pKa = 10.53 LLVAMGFLVEE267 pKa = 4.24 PFGLSGIFLRR277 pKa = 11.84 TGVVAAACYY286 pKa = 10.54 NYY288 pKa = 8.87 GTNSKK293 pKa = 10.34 GFAEE297 pKa = 4.3 MMALLSLAANCVSTVIVGGFFPGEE321 pKa = 3.79 KK322 pKa = 10.23 DD323 pKa = 3.32 NAQSSPVILLEE334 pKa = 4.1 GLAGQMQNFCEE345 pKa = 4.34 TTLVSVGKK353 pKa = 8.44 TCTAVNAISTCCGNLKK369 pKa = 10.4 ALAGRR374 pKa = 11.84 ILGMLRR380 pKa = 11.84 DD381 pKa = 5.28 FIWKK385 pKa = 8.43 TLGFEE390 pKa = 4.35 TRR392 pKa = 11.84 FLADD396 pKa = 3.13 ASLLFGEE403 pKa = 6.0 DD404 pKa = 2.64 VDD406 pKa = 4.17 GWLKK410 pKa = 10.9 AISDD414 pKa = 4.17 LRR416 pKa = 11.84 DD417 pKa = 3.13 QFIAKK422 pKa = 9.47 SYY424 pKa = 9.86 CSQDD428 pKa = 2.59 EE429 pKa = 4.15 MMQILVLLEE438 pKa = 4.24 KK439 pKa = 10.09 GRR441 pKa = 11.84 QMRR444 pKa = 11.84 KK445 pKa = 9.55 SGLSKK450 pKa = 10.96 GGISPAIINLILKK463 pKa = 8.84 GINDD467 pKa = 4.4 LEE469 pKa = 4.07 QLNRR473 pKa = 11.84 SCSVQGVRR481 pKa = 11.84 GVRR484 pKa = 11.84 KK485 pKa = 8.55 MPFTIFFQGKK495 pKa = 8.67 SRR497 pKa = 11.84 TGKK500 pKa = 10.05 SLLMSQVTKK509 pKa = 10.77 DD510 pKa = 3.78 FQDD513 pKa = 3.21 HH514 pKa = 6.33 YY515 pKa = 10.73 GLGGEE520 pKa = 4.57 TVYY523 pKa = 11.12 SRR525 pKa = 11.84 NPCDD529 pKa = 3.89 QYY531 pKa = 9.14 WSGYY535 pKa = 7.99 RR536 pKa = 11.84 RR537 pKa = 11.84 QPFVLMDD544 pKa = 3.98 DD545 pKa = 4.6 FAAVVTEE552 pKa = 4.66 PSAEE556 pKa = 3.65 AQMINLISSAPYY568 pKa = 8.06 PLNMAGLEE576 pKa = 4.1 EE577 pKa = 4.8 KK578 pKa = 10.56 GICFDD583 pKa = 3.6 SQFVFVSTNFLEE595 pKa = 4.44 VSPEE599 pKa = 3.62 AKK601 pKa = 10.38 VRR603 pKa = 11.84 DD604 pKa = 3.74 DD605 pKa = 3.51 EE606 pKa = 4.4 AFKK609 pKa = 10.93 NRR611 pKa = 11.84 RR612 pKa = 11.84 HH613 pKa = 6.5 VIVQVSNDD621 pKa = 3.39 PAKK624 pKa = 10.74 AYY626 pKa = 9.86 DD627 pKa = 3.72 AANFASNQIYY637 pKa = 9.0 TILAWKK643 pKa = 9.69 DD644 pKa = 3.07 GRR646 pKa = 11.84 YY647 pKa = 8.22 NTVCVIEE654 pKa = 5.73 DD655 pKa = 3.53 YY656 pKa = 11.38 DD657 pKa = 3.88 EE658 pKa = 4.39 LVAYY662 pKa = 10.58 LLTRR666 pKa = 11.84 SQQHH670 pKa = 6.31 AEE672 pKa = 4.04 EE673 pKa = 4.32 QEE675 pKa = 4.12 KK676 pKa = 10.95 NLANMMKK683 pKa = 10.02 SATFEE688 pKa = 4.28 SHH690 pKa = 6.75 FKK692 pKa = 10.99 SLVEE696 pKa = 3.84 VLEE699 pKa = 4.59 LGSMISAGFDD709 pKa = 3.13 IIRR712 pKa = 11.84 PEE714 pKa = 4.06 KK715 pKa = 10.53 LPSEE719 pKa = 4.28 AKK721 pKa = 9.47 EE722 pKa = 3.81 KK723 pKa = 10.45 RR724 pKa = 11.84 VLYY727 pKa = 9.49 SIPYY731 pKa = 8.88 NGEE734 pKa = 3.6 YY735 pKa = 10.57 CNALIDD741 pKa = 4.34 DD742 pKa = 4.99 NYY744 pKa = 11.55 NVTCWFGEE752 pKa = 4.41 CVGNPEE758 pKa = 4.01 QLSKK762 pKa = 11.33 YY763 pKa = 7.84 SEE765 pKa = 3.98 KK766 pKa = 10.12 MLLGAYY772 pKa = 8.05 EE773 pKa = 4.49 FLLCSEE779 pKa = 4.58 SLNVVIQAHH788 pKa = 6.13 LKK790 pKa = 10.54 EE791 pKa = 4.86 MVCPHH796 pKa = 6.88 HH797 pKa = 6.92 YY798 pKa = 10.58 DD799 pKa = 3.52 KK800 pKa = 11.26 EE801 pKa = 4.48 LNFIGKK807 pKa = 9.23 IGEE810 pKa = 4.5 TYY812 pKa = 10.46 YY813 pKa = 10.81 HH814 pKa = 6.09 NQMVSNIGSMQKK826 pKa = 6.82 WHH828 pKa = 6.64 RR829 pKa = 11.84 AILFGIGVLLGKK841 pKa = 10.11 EE842 pKa = 4.39 KK843 pKa = 10.87 EE844 pKa = 4.29 KK845 pKa = 9.57 TWYY848 pKa = 8.97 QVQVANVKK856 pKa = 9.43 QALYY860 pKa = 11.34 DD861 pKa = 3.51 MYY863 pKa = 10.77 TKK865 pKa = 10.32 EE866 pKa = 3.92 IRR868 pKa = 11.84 DD869 pKa = 3.45 WPMPIKK875 pKa = 8.78 VTCGIVLAAIGGSAFWKK892 pKa = 10.22 VFQQLVGSGNGPVLMGVAAGAFSAEE917 pKa = 4.13 PQSRR921 pKa = 11.84 KK922 pKa = 9.49 PNRR925 pKa = 11.84 FDD927 pKa = 3.24 MQQYY931 pKa = 10.03 RR932 pKa = 11.84 YY933 pKa = 10.84 NNVPLKK939 pKa = 10.51 RR940 pKa = 11.84 RR941 pKa = 11.84 VWADD945 pKa = 2.84 AQMSLDD951 pKa = 3.74 QSSVAIMSKK960 pKa = 10.2 CRR962 pKa = 11.84 ANLVFGGTNLQIVMVPGRR980 pKa = 11.84 RR981 pKa = 11.84 FLACKK986 pKa = 10.26 HH987 pKa = 5.84 FFTHH991 pKa = 6.71 IKK993 pKa = 8.56 TKK995 pKa = 10.48 LRR997 pKa = 11.84 VEE999 pKa = 3.8 IVMDD1003 pKa = 3.47 GRR1005 pKa = 11.84 RR1006 pKa = 11.84 YY1007 pKa = 9.36 YY1008 pKa = 10.83 HH1009 pKa = 6.42 QFDD1012 pKa = 3.63 PANIYY1017 pKa = 9.63 DD1018 pKa = 4.04 IPDD1021 pKa = 3.83 SEE1023 pKa = 4.81 LVLYY1027 pKa = 7.74 SHH1029 pKa = 7.81 PSLEE1033 pKa = 4.68 DD1034 pKa = 3.35 VSHH1037 pKa = 6.21 SCWDD1041 pKa = 4.04 LFCWDD1046 pKa = 4.69 PDD1048 pKa = 3.61 KK1049 pKa = 11.03 EE1050 pKa = 4.3 LPSVFGADD1058 pKa = 4.01 FLSCKK1063 pKa = 9.36 YY1064 pKa = 10.75 NKK1066 pKa = 10.18 FGGFYY1071 pKa = 8.99 EE1072 pKa = 4.29 AQYY1075 pKa = 11.75 ADD1077 pKa = 2.81 IKK1079 pKa = 11.24 VRR1081 pKa = 11.84 TKK1083 pKa = 10.68 KK1084 pKa = 10.35 EE1085 pKa = 3.81 CLTIQSGNYY1094 pKa = 7.02 VNKK1097 pKa = 8.99 VSRR1100 pKa = 11.84 YY1101 pKa = 9.52 LEE1103 pKa = 4.32 YY1104 pKa = 10.19 EE1105 pKa = 3.99 APTIPEE1111 pKa = 4.32 DD1112 pKa = 3.94 CGSLVIAHH1120 pKa = 7.28 IGGKK1124 pKa = 9.24 HH1125 pKa = 6.0 KK1126 pKa = 10.58 IVGVHH1131 pKa = 4.92 VAGIQGKK1138 pKa = 9.26 IGCASLLPPLEE1149 pKa = 5.35 PIAQAQGAEE1158 pKa = 4.39 EE1159 pKa = 4.37 YY1160 pKa = 10.9 FDD1162 pKa = 4.21 FLPAEE1167 pKa = 4.51 EE1168 pKa = 4.45 NVSSGVAMVAGLKK1181 pKa = 9.93 QGVYY1185 pKa = 10.08 IPLPTKK1191 pKa = 9.36 TALVEE1196 pKa = 4.59 TPSEE1200 pKa = 3.93 WHH1202 pKa = 7.18 LDD1204 pKa = 3.94 TPCDD1208 pKa = 3.8 KK1209 pKa = 10.81 VPSILVPTDD1218 pKa = 3.15 PRR1220 pKa = 11.84 IPAQHH1225 pKa = 6.58 EE1226 pKa = 4.87 GYY1228 pKa = 10.54 DD1229 pKa = 3.74 PAKK1232 pKa = 10.55 SGVSKK1237 pKa = 10.73 YY1238 pKa = 8.76 SQPMSALDD1246 pKa = 3.78 PEE1248 pKa = 4.77 LLGEE1252 pKa = 4.28 VANDD1256 pKa = 3.98 VLEE1259 pKa = 4.46 LWHH1262 pKa = 7.24 DD1263 pKa = 4.11 CAVDD1267 pKa = 3.11 WDD1269 pKa = 4.41 DD1270 pKa = 4.43 FGEE1273 pKa = 4.36 VSLEE1277 pKa = 3.85 EE1278 pKa = 4.21 ALNGCEE1284 pKa = 3.46 GVEE1287 pKa = 4.0 YY1288 pKa = 10.15 MEE1290 pKa = 5.74 RR1291 pKa = 11.84 IPLATSEE1298 pKa = 4.43 GFPHH1302 pKa = 6.64 ILSRR1306 pKa = 11.84 NGKK1309 pKa = 8.54 EE1310 pKa = 3.41 KK1311 pKa = 10.19 GKK1313 pKa = 10.47 RR1314 pKa = 11.84 RR1315 pKa = 11.84 FVQGDD1320 pKa = 3.7 DD1321 pKa = 4.12 CVVSLIPGTTVAKK1334 pKa = 10.34 AYY1336 pKa = 10.1 EE1337 pKa = 4.04 EE1338 pKa = 4.77 LEE1340 pKa = 4.25 ASAHH1344 pKa = 5.54 RR1345 pKa = 11.84 FVPALVGIEE1354 pKa = 4.15 CPKK1357 pKa = 10.8 DD1358 pKa = 3.28 EE1359 pKa = 4.93 KK1360 pKa = 11.41 LPMRR1364 pKa = 11.84 KK1365 pKa = 9.22 VFDD1368 pKa = 3.86 KK1369 pKa = 10.99 PKK1371 pKa = 9.17 TRR1373 pKa = 11.84 CFTILPMEE1381 pKa = 4.41 YY1382 pKa = 10.16 NLVVRR1387 pKa = 11.84 RR1388 pKa = 11.84 KK1389 pKa = 8.91 FLNFVRR1395 pKa = 11.84 FIMANRR1401 pKa = 11.84 HH1402 pKa = 5.42 RR1403 pKa = 11.84 LSCQVGINPYY1413 pKa = 9.64 SMEE1416 pKa = 3.87 WSRR1419 pKa = 11.84 LAARR1423 pKa = 11.84 MKK1425 pKa = 10.77 EE1426 pKa = 3.76 KK1427 pKa = 11.07 GNDD1430 pKa = 3.67 VLCCDD1435 pKa = 3.82 YY1436 pKa = 11.65 SSFDD1440 pKa = 3.64 GLLSKK1445 pKa = 10.75 QVMDD1449 pKa = 5.6 VIASMINEE1457 pKa = 4.25 LCGGEE1462 pKa = 4.29 DD1463 pKa = 3.21 QLKK1466 pKa = 9.18 NARR1469 pKa = 11.84 RR1470 pKa = 11.84 NLLMACCSRR1479 pKa = 11.84 LAICKK1484 pKa = 7.49 NTVWRR1489 pKa = 11.84 VEE1491 pKa = 4.02 CGIPSGFPMTVIVNSIFNEE1510 pKa = 3.52 ILIRR1514 pKa = 11.84 YY1515 pKa = 7.82 HH1516 pKa = 5.75 YY1517 pKa = 9.26 KK1518 pKa = 10.48 KK1519 pKa = 10.75 LMRR1522 pKa = 11.84 EE1523 pKa = 3.98 QQAPEE1528 pKa = 4.23 LMVQSFDD1535 pKa = 3.61 KK1536 pKa = 11.33 LIGLVTYY1543 pKa = 10.88 GDD1545 pKa = 4.46 DD1546 pKa = 3.94 NLISVNAVVTPYY1558 pKa = 10.85 FDD1560 pKa = 3.77 GKK1562 pKa = 10.78 KK1563 pKa = 9.95 LKK1565 pKa = 10.38 QSLAQGGVTITDD1577 pKa = 3.93 GKK1579 pKa = 10.86 DD1580 pKa = 3.01 KK1581 pKa = 11.02 TSLEE1585 pKa = 4.09 LPFRR1589 pKa = 11.84 RR1590 pKa = 11.84 LEE1592 pKa = 3.95 EE1593 pKa = 4.15 CDD1595 pKa = 3.27 FLKK1598 pKa = 10.13 RR1599 pKa = 11.84 TFVQRR1604 pKa = 11.84 SSTIWDD1610 pKa = 3.56 APEE1613 pKa = 4.96 DD1614 pKa = 4.01 KK1615 pKa = 10.97 ASLWSQLHH1623 pKa = 5.21 YY1624 pKa = 11.14 VNCNNCEE1631 pKa = 3.98 KK1632 pKa = 10.55 EE1633 pKa = 4.23 VAYY1636 pKa = 8.6 LTNVVNVLRR1645 pKa = 11.84 EE1646 pKa = 4.28 LYY1648 pKa = 8.87 MHH1650 pKa = 6.87 SPRR1653 pKa = 11.84 EE1654 pKa = 3.96 ATEE1657 pKa = 3.78 FRR1659 pKa = 11.84 RR1660 pKa = 11.84 KK1661 pKa = 9.06 VLKK1664 pKa = 10.05 KK1665 pKa = 10.62 VSWITSGDD1673 pKa = 4.05 LPTLAQLQEE1682 pKa = 4.2 FYY1684 pKa = 10.67 EE1685 pKa = 4.27 YY1686 pKa = 10.68 QRR1688 pKa = 11.84 QQGGADD1694 pKa = 3.58 NNDD1697 pKa = 3.35 TCDD1700 pKa = 3.83 LLTSVDD1706 pKa = 4.14 LLGPPLSFEE1715 pKa = 4.08 KK1716 pKa = 10.67 EE1717 pKa = 3.9 AMHH1720 pKa = 6.14 GCKK1723 pKa = 10.05 VSEE1726 pKa = 4.47 EE1727 pKa = 4.09 IVTKK1731 pKa = 10.85 NLAYY1735 pKa = 10.78 YY1736 pKa = 9.95 DD1737 pKa = 4.25 FKK1739 pKa = 11.28 RR1740 pKa = 11.84 KK1741 pKa = 10.53 GEE1743 pKa = 4.16 DD1744 pKa = 3.0 EE1745 pKa = 4.5 VVFLFNTLYY1754 pKa = 9.95 PQSSLPDD1761 pKa = 3.62 GCHH1764 pKa = 5.6 SVTWSQGSGRR1774 pKa = 11.84 GGLPTQSWMSYY1785 pKa = 9.63 NISRR1789 pKa = 11.84 KK1790 pKa = 9.8 DD1791 pKa = 3.43 SNINKK1796 pKa = 9.82 IIRR1799 pKa = 11.84 TAVSSKK1805 pKa = 10.49 KK1806 pKa = 10.18 RR1807 pKa = 11.84 VIFCARR1813 pKa = 11.84 DD1814 pKa = 3.19 NMVPVNIVALLCAVRR1829 pKa = 11.84 NKK1831 pKa = 10.5 LMPTAVSNATLVKK1844 pKa = 10.76 VMEE1847 pKa = 4.14 NAKK1850 pKa = 10.29 AFKK1853 pKa = 10.12 FLPEE1857 pKa = 3.9 EE1858 pKa = 4.1 FNFAFSDD1865 pKa = 3.75 VV1866 pKa = 3.44
Molecular weight: 209.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.618
IPC2_protein 5.703
IPC_protein 5.741
Toseland 5.97
ProMoST 6.059
Dawson 5.957
Bjellqvist 5.995
Wikipedia 5.944
Rodwell 5.931
Grimsley 6.046
Solomon 5.957
Lehninger 5.944
Nozaki 6.211
DTASelect 6.389
Thurlkill 6.364
EMBOSS 6.326
Sillero 6.313
Patrickios 3.923
IPC_peptide 5.97
IPC2_peptide 6.275
IPC2.peptide.svr19 6.299
Protein with the highest isoelectric point:
>sp|P03599-2|POL2-2_CPMVS Isoform of P03599 Isoform 2 of RNA2 polyprotein OS=Cowpea mosaic virus (strain SB) OX=928299 PE=1 SV=1
HHH2 pKa = 6.97 EE3 pKa = 4.54 SSTLSDDD10 pKa = 3.15 ASAPVQCFLSLKKK23 pKa = 9.77 NQRR26 pKa = 11.84 SLCGHHH32 pKa = 5.37 VRR34 pKa = 11.84 RR35 pKa = 11.84 H
Molecular weight: 4.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.184
IPC2_protein 9.663
IPC_protein 11.155
Toseland 11.359
ProMoST 11.813
Dawson 11.359
Bjellqvist 11.33
Wikipedia 11.813
Rodwell 11.096
Grimsley 11.403
Solomon 11.828
Lehninger 11.74
Nozaki 11.359
DTASelect 11.33
Thurlkill 11.359
EMBOSS 11.842
Sillero 11.359
Patrickios 10.979
IPC_peptide 11.828
IPC2_peptide 10.833
IPC2.peptide.svr19 9.693
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
1
4
3877
36
1866
969.3
108.12
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.299 ± 0.088
2.605 ± 0.151
4.823 ± 0.13
5.597 ± 0.582
5.133 ± 0.234
6.397 ± 0.108
1.831 ± 0.112
5.417 ± 0.286
5.494 ± 0.338
8.589 ± 0.58
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.94 ± 0.165
4.669 ± 0.242
4.823 ± 0.425
3.766 ± 0.3
4.643 ± 0.323
7.944 ± 0.527
5.52 ± 0.847
7.738 ± 0.349
1.625 ± 0.033
3.147 ± 0.371
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here