candidate division MSBL1 archaeon SCGC-AAA259D14

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Euryarchaeota incertae sedis; candidate division MSBL1

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 885 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A133U7N2|A0A133U7N2_9EURY Uncharacterized protein OS=candidate division MSBL1 archaeon SCGC-AAA259D14 OX=1698261 GN=AKJ62_01350 PE=4 SV=1
EEE2 pKa = 4.45EE3 pKa = 4.16EE4 pKa = 3.77DDD6 pKa = 3.68EE7 pKa = 5.17VGKKK11 pKa = 9.81IRR13 pKa = 11.84ALEEE17 pKa = 3.91LGIDDD22 pKa = 3.58EEE24 pKa = 4.38EEE26 pKa = 3.7KK27 pKa = 11.13DD28 pKa = 3.5LSRR31 pKa = 11.84EEE33 pKa = 4.08EE34 pKa = 5.04EEE36 pKa = 4.72EE37 pKa = 4.49PTISNMIHHH46 pKa = 6.35DDD48 pKa = 3.54RR49 pKa = 11.84PLEEE53 pKa = 4.12KK54 pKa = 10.02IDDD57 pKa = 3.35DDD59 pKa = 3.58GQSEEE64 pKa = 4.65CEEE67 pKa = 3.92CGDDD71 pKa = 3.88EE72 pKa = 4.21EE73 pKa = 4.73RR74 pKa = 11.84TVILGGEEE82 pKa = 4.24EE83 pKa = 4.51YY84 pKa = 10.56EE85 pKa = 4.61VPADDD90 pKa = 4.53IVNAGLRR97 pKa = 11.84AVSRR101 pKa = 11.84VPSDDD106 pKa = 4.19EEE108 pKa = 4.43CSSSGEEE115 pKa = 4.13YY116 pKa = 9.65CCDDD120 pKa = 2.96

Molecular weight: 13.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A133U4H9|A0A133U4H9_9EURY Cold-shock protein OS=candidate division MSBL1 archaeon SCGC-AAA259D14 OX=1698261 GN=AKJ62_03760 PE=4 SV=1
MM1 pKa = 7.4SHH3 pKa = 6.35GLSRR7 pKa = 11.84NRR9 pKa = 11.84VGGKK13 pKa = 9.84RR14 pKa = 11.84NIRR17 pKa = 11.84PAEE20 pKa = 3.78KK21 pKa = 9.63RR22 pKa = 11.84NKK24 pKa = 9.59YY25 pKa = 8.47RR26 pKa = 11.84TNQEE30 pKa = 3.68YY31 pKa = 11.2GEE33 pKa = 4.17FNQRR37 pKa = 11.84NRR39 pKa = 11.84TEE41 pKa = 4.18SDD43 pKa = 2.87RR44 pKa = 11.84LLYY47 pKa = 9.76PWSSGGNKK55 pKa = 9.42EE56 pKa = 3.96VTQEE60 pKa = 3.78VSEE63 pKa = 4.23SSRR66 pKa = 11.84RR67 pKa = 11.84YY68 pKa = 9.9IRR70 pKa = 11.84TTT72 pKa = 2.93

Molecular weight: 8.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

885

0

885

192968

36

1111

218.0

24.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.01 ± 0.083

1.082 ± 0.044

5.651 ± 0.075

10.073 ± 0.118

4.009 ± 0.074

7.588 ± 0.098

1.603 ± 0.037

7.193 ± 0.094

7.576 ± 0.116

9.006 ± 0.099

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.223 ± 0.034

3.738 ± 0.067

4.075 ± 0.05

2.228 ± 0.046

5.742 ± 0.079

6.402 ± 0.079

4.656 ± 0.058

7.067 ± 0.077

1.129 ± 0.038

2.949 ± 0.046
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski