Alicyclobacillus hesperidum

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Alicyclobacillaceae; Alicyclobacillus

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2843 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H2SG27|A0A1H2SG27_9BACL Peptidoglycan/xylan/chitin deacetylase PgdA/CDA1 family OS=Alicyclobacillus hesperidum OX=89784 GN=SAMN04489725_10427 PE=4 SV=1
MM1 pKa = 7.51VVILLVVIVILLVVLLARR19 pKa = 11.84RR20 pKa = 11.84NQAGNGYY27 pKa = 9.79RR28 pKa = 11.84RR29 pKa = 11.84GPFQNNGFRR38 pKa = 11.84DD39 pKa = 3.6NQYY42 pKa = 11.01YY43 pKa = 10.75DD44 pKa = 3.28VTPPYY49 pKa = 9.13STPGYY54 pKa = 8.13TGGFGRR60 pKa = 11.84TGSFLGGMAAGALLSYY76 pKa = 10.62LLEE79 pKa = 4.03QGRR82 pKa = 11.84IDD84 pKa = 3.46MMQYY88 pKa = 11.55DD89 pKa = 4.96MFRR92 pKa = 11.84QMEE95 pKa = 4.35DD96 pKa = 2.78QQMINEE102 pKa = 4.85LMQQNIIQQDD112 pKa = 3.95EE113 pKa = 3.97IDD115 pKa = 3.5QLQRR119 pKa = 11.84EE120 pKa = 4.42IGMDD124 pKa = 3.43QYY126 pKa = 12.02GGADD130 pKa = 3.88WNNGPYY136 pKa = 10.28DD137 pKa = 3.51QGPYY141 pKa = 10.88GDD143 pKa = 4.74MGPNSWDD150 pKa = 3.51NQVDD154 pKa = 4.43FPTNDD159 pKa = 4.15DD160 pKa = 3.79PFQNDD165 pKa = 3.38SFNDD169 pKa = 3.55NNGDD173 pKa = 3.21NWVV176 pKa = 3.29

Molecular weight: 19.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H2X5R3|A0A1H2X5R3_9BACL Uncharacterized protein OS=Alicyclobacillus hesperidum OX=89784 GN=SAMN04489725_11852 PE=4 SV=1
MM1 pKa = 7.52KK2 pKa = 8.55PTYY5 pKa = 9.7QPNVRR10 pKa = 11.84KK11 pKa = 9.84RR12 pKa = 11.84KK13 pKa = 8.89KK14 pKa = 8.99NHH16 pKa = 5.02GFRR19 pKa = 11.84KK20 pKa = 9.98RR21 pKa = 11.84MSTKK25 pKa = 9.46NGRR28 pKa = 11.84RR29 pKa = 11.84VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.71GRR39 pKa = 11.84KK40 pKa = 8.73VLSAA44 pKa = 4.05

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2843

0

2843

851207

28

2691

299.4

33.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.261 ± 0.059

0.965 ± 0.014

5.136 ± 0.04

5.863 ± 0.053

3.72 ± 0.032

7.715 ± 0.045

2.494 ± 0.024

5.726 ± 0.037

3.364 ± 0.038

9.889 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.657 ± 0.022

2.944 ± 0.036

4.545 ± 0.032

4.107 ± 0.029

6.534 ± 0.063

5.686 ± 0.044

5.509 ± 0.041

8.467 ± 0.037

1.458 ± 0.022

2.96 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski