Wuhan heteroptera virus 2

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KEV8|A0A1L3KEV8_9VIRU Capsid protein OS=Wuhan heteroptera virus 2 OX=1923702 PE=3 SV=1
MM1 pKa = 7.78DD2 pKa = 4.59FDD4 pKa = 4.08VRR6 pKa = 11.84VGRR9 pKa = 11.84ALKK12 pKa = 9.9EE13 pKa = 4.07CQPDD17 pKa = 3.7SVTGLGYY24 pKa = 10.23FRR26 pKa = 11.84NYY28 pKa = 7.2QTIGDD33 pKa = 4.21AFGHH37 pKa = 5.77EE38 pKa = 4.45CGFYY42 pKa = 10.61TNAHH46 pKa = 6.42NIAILVNIVRR56 pKa = 11.84EE57 pKa = 4.25KK58 pKa = 10.74IRR60 pKa = 11.84FLDD63 pKa = 3.3SGGQYY68 pKa = 10.82GHH70 pKa = 6.87GPDD73 pKa = 3.84DD74 pKa = 3.89AKK76 pKa = 11.51VFIKK80 pKa = 10.54FEE82 pKa = 3.94ATKK85 pKa = 10.1QSKK88 pKa = 8.63VQEE91 pKa = 3.8QRR93 pKa = 11.84YY94 pKa = 8.96RR95 pKa = 11.84LIWSLSVEE103 pKa = 4.1DD104 pKa = 4.32QIVDD108 pKa = 3.46RR109 pKa = 11.84LLFGHH114 pKa = 7.48AFRR117 pKa = 11.84AEE119 pKa = 4.12VNHH122 pKa = 5.7FTEE125 pKa = 4.99TTSKK129 pKa = 9.58TGWSPLPGGYY139 pKa = 9.8SYY141 pKa = 11.42FNSTFNGHH149 pKa = 5.76VLATDD154 pKa = 3.87ASAFDD159 pKa = 3.51WTVQNWMVAMLYY171 pKa = 8.2DD172 pKa = 3.64TLMDD176 pKa = 3.53RR177 pKa = 11.84TRR179 pKa = 11.84GNTEE183 pKa = 3.45SYY185 pKa = 11.12KK186 pKa = 10.78RR187 pKa = 11.84MAFNRR192 pKa = 11.84IQQVLGPEE200 pKa = 4.29CVVRR204 pKa = 11.84LPNGRR209 pKa = 11.84RR210 pKa = 11.84FRR212 pKa = 11.84QRR214 pKa = 11.84VFGVMKK220 pKa = 10.56SGWLLTLMANTNVMLASSLCAWARR244 pKa = 11.84AAPAYY249 pKa = 10.5FEE251 pKa = 4.24ATPDD255 pKa = 3.35GLYY258 pKa = 10.32PDD260 pKa = 5.11MPLSWGMGDD269 pKa = 4.07DD270 pKa = 6.21VIMKK274 pKa = 9.04WDD276 pKa = 3.64PTLSPEE282 pKa = 4.15PYY284 pKa = 9.59VRR286 pKa = 11.84EE287 pKa = 4.05LNRR290 pKa = 11.84TGLKK294 pKa = 9.45VKK296 pKa = 10.2QYY298 pKa = 8.01SHH300 pKa = 6.02EE301 pKa = 4.15RR302 pKa = 11.84EE303 pKa = 3.8FAGFRR308 pKa = 11.84IQPSSGDD315 pKa = 3.49QQPTVTPIYY324 pKa = 8.24TAKK327 pKa = 10.12HH328 pKa = 4.34MTMIAYY334 pKa = 7.92TPRR337 pKa = 11.84EE338 pKa = 4.16RR339 pKa = 11.84LEE341 pKa = 4.16EE342 pKa = 3.76MAIAYY347 pKa = 8.84GLIYY351 pKa = 10.47ALSDD355 pKa = 3.49SASPQMKK362 pKa = 10.11KK363 pKa = 10.7FIDD366 pKa = 4.38DD367 pKa = 3.59YY368 pKa = 11.45SPYY371 pKa = 8.9GSSYY375 pKa = 9.79FEE377 pKa = 3.8HH378 pKa = 7.29WARR381 pKa = 11.84GQPLPGNDD389 pKa = 3.59GPRR392 pKa = 11.84VRR394 pKa = 11.84MQLDD398 pKa = 3.07NAYY401 pKa = 10.51ALARR405 pKa = 11.84TQDD408 pKa = 3.21QQ409 pKa = 3.26

Molecular weight: 46.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KEV8|A0A1L3KEV8_9VIRU Capsid protein OS=Wuhan heteroptera virus 2 OX=1923702 PE=3 SV=1
MM1 pKa = 6.87VRR3 pKa = 11.84SRR5 pKa = 11.84KK6 pKa = 7.89KK7 pKa = 8.98TKK9 pKa = 8.7PTSKK13 pKa = 10.46LVAEE17 pKa = 4.63VAAATKK23 pKa = 10.21AVQALKK29 pKa = 10.43IGTPARR35 pKa = 11.84KK36 pKa = 9.1RR37 pKa = 11.84KK38 pKa = 8.67PRR40 pKa = 11.84SRR42 pKa = 11.84VPRR45 pKa = 11.84QRR47 pKa = 11.84GAGNVYY53 pKa = 10.34SQANDD58 pKa = 3.02ATVVFTRR65 pKa = 11.84SEE67 pKa = 3.95MLEE70 pKa = 4.44AITVSDD76 pKa = 2.97QGTYY80 pKa = 10.36LKK82 pKa = 10.51HH83 pKa = 6.59FKK85 pKa = 8.7ITPATFPLLSKK96 pKa = 10.28IAQHH100 pKa = 6.62FDD102 pKa = 2.89QSQWLAVEE110 pKa = 4.81VYY112 pKa = 6.51WTSDD116 pKa = 2.75VGTTTGGSITYY127 pKa = 10.17GFDD130 pKa = 3.25WDD132 pKa = 3.73PTTAEE137 pKa = 4.55SKK139 pKa = 10.52NKK141 pKa = 8.31TRR143 pKa = 11.84AAITALTPSHH153 pKa = 6.41TGAVYY158 pKa = 10.26RR159 pKa = 11.84QTDD162 pKa = 3.88TAPLKK167 pKa = 10.72APKK170 pKa = 9.95NQLDD174 pKa = 3.56TLKK177 pKa = 10.07WYY179 pKa = 10.05KK180 pKa = 10.94HH181 pKa = 5.54NDD183 pKa = 2.92ATASADD189 pKa = 3.64SNLIAPCTVSVMASSTPKK207 pKa = 10.39LAIGVLWCRR216 pKa = 11.84YY217 pKa = 9.09RR218 pKa = 11.84IRR220 pKa = 11.84LRR222 pKa = 11.84GTTSXRR228 pKa = 11.84QLYY231 pKa = 9.34QLQTLSSVWTHH242 pKa = 7.07RR243 pKa = 11.84DD244 pKa = 3.3TQSAAWNTRR253 pKa = 11.84VWEE256 pKa = 4.12TDD258 pKa = 2.68EE259 pKa = 4.75LAPSKK264 pKa = 10.5PRR266 pKa = 11.84VYY268 pKa = 10.29AYY270 pKa = 9.75KK271 pKa = 10.37AATRR275 pKa = 11.84PVEE278 pKa = 3.9LPVRR282 pKa = 11.84SEE284 pKa = 3.62PTKK287 pKa = 10.56RR288 pKa = 11.84DD289 pKa = 3.26LSVQEE294 pKa = 4.72VKK296 pKa = 10.85QKK298 pKa = 11.67DD299 pKa = 3.53NMDD302 pKa = 3.96NVWSFNAGRR311 pKa = 11.84LPTPHH316 pKa = 6.89VIWTCQLYY324 pKa = 10.26VPVSFQPLAGTIADD338 pKa = 3.82ICFDD342 pKa = 3.47MQLVHH347 pKa = 6.72SHH349 pKa = 6.96DD350 pKa = 4.09RR351 pKa = 11.84LPTDD355 pKa = 3.15MTITLAPQALLEE367 pKa = 4.26SGYY370 pKa = 9.4VQEE373 pKa = 5.14STPSMYY379 pKa = 10.85LGGRR383 pKa = 11.84AMTVFSMLIGKK394 pKa = 9.62LMDD397 pKa = 3.21WTKK400 pKa = 11.16EE401 pKa = 3.7MSVLIGFEE409 pKa = 4.02FDD411 pKa = 4.53FNAFDD416 pKa = 3.92SSATQSLTFSYY427 pKa = 10.91NVTVRR432 pKa = 11.84LTSDD436 pKa = 3.7GASKK440 pKa = 10.91ASDD443 pKa = 3.45QQQLICKK450 pKa = 8.6SCQGVAEE457 pKa = 4.46IVPSRR462 pKa = 11.84PSSPGIPFYY471 pKa = 11.28GGVDD475 pKa = 3.11PGYY478 pKa = 10.11ISNGDD483 pKa = 3.7FVFVLPDD490 pKa = 3.56GSEE493 pKa = 4.07LPSRR497 pKa = 11.84PPINN501 pKa = 3.28

Molecular weight: 55.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1658

409

748

552.7

61.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.263 ± 0.208

1.206 ± 0.093

4.885 ± 0.584

4.403 ± 0.416

3.317 ± 0.741

5.911 ± 0.629

1.93 ± 0.131

4.282 ± 0.211

4.584 ± 0.381

8.323 ± 0.553

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.714 ± 0.451

3.739 ± 0.326

6.092 ± 0.28

5.609 ± 0.425

6.212 ± 0.346

9.59 ± 1.458

7.6 ± 0.808

5.971 ± 0.575

1.689 ± 0.322

3.619 ± 0.574
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski