Salmonella phage LPSE1
Average proteome isoelectric point is 6.42
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 59 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L7DRT9|A0A1L7DRT9_9CAUD Uncharacterized protein OS=Salmonella phage LPSE1 OX=1929963 GN=LPSE_00028 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 10.39 VYY4 pKa = 10.13 IVSGWFHH11 pKa = 7.01 YY12 pKa = 10.86 DD13 pKa = 2.84 GADD16 pKa = 3.01 ILGVYY21 pKa = 10.2 SSEE24 pKa = 4.2 EE25 pKa = 3.85 KK26 pKa = 11.08 AEE28 pKa = 3.98 AAAEE32 pKa = 4.0 QEE34 pKa = 4.22 RR35 pKa = 11.84 DD36 pKa = 3.19 KK37 pKa = 11.6 SDD39 pKa = 3.34 YY40 pKa = 11.38 DD41 pKa = 4.5 GIDD44 pKa = 3.16 VCEE47 pKa = 4.45 WEE49 pKa = 4.38 VQEE52 pKa = 4.76
Molecular weight: 5.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.81
IPC2_protein 4.037
IPC_protein 3.91
Toseland 3.732
ProMoST 4.012
Dawson 3.884
Bjellqvist 4.126
Wikipedia 3.795
Rodwell 3.757
Grimsley 3.656
Solomon 3.859
Lehninger 3.821
Nozaki 4.012
DTASelect 4.151
Thurlkill 3.783
EMBOSS 3.808
Sillero 4.024
Patrickios 1.901
IPC_peptide 3.872
IPC2_peptide 4.012
IPC2.peptide.svr19 3.968
Protein with the highest isoelectric point:
>tr|A0A1L7DS97|A0A1L7DS97_9CAUD Tailspike protein OS=Salmonella phage LPSE1 OX=1929963 GN=LPSE_00072 PE=4 SV=1
MM1 pKa = 7.52 LACDD5 pKa = 3.52 SSSCQRR11 pKa = 11.84 RR12 pKa = 11.84 MMAARR17 pKa = 11.84 ILMLYY22 pKa = 9.86 PEE24 pKa = 3.85 IRR26 pKa = 11.84 INVFSLSKK34 pKa = 10.73 RR35 pKa = 11.84 NTLLGLPPFEE45 pKa = 5.04 GKK47 pKa = 8.24 TFSSKK52 pKa = 10.39 LRR54 pKa = 11.84 EE55 pKa = 4.02 SSCAIFNISRR65 pKa = 11.84 RR66 pKa = 11.84 TLSCSFPVNSAISRR80 pKa = 11.84 LDD82 pKa = 3.4 MVCASLTFINSFMVSSPLVGVDD104 pKa = 2.62 IFNFEE109 pKa = 3.86 HH110 pKa = 5.95 SHH112 pKa = 6.67 RR113 pKa = 11.84 SNQILYY119 pKa = 9.99 RR120 pKa = 11.84 DD121 pKa = 3.76 DD122 pKa = 3.55 KK123 pKa = 10.97 KK124 pKa = 10.94 RR125 pKa = 11.84 PRR127 pKa = 11.84 KK128 pKa = 10.2 GPFSHH133 pKa = 7.17 SSILLRR139 pKa = 11.84 YY140 pKa = 8.38 FRR142 pKa = 11.84 NFSRR146 pKa = 11.84 LSIASAKK153 pKa = 9.74 SVPVSLCPRR162 pKa = 11.84 KK163 pKa = 9.1 PVHH166 pKa = 6.41 NSFFNACCSAGSRR179 pKa = 11.84 ISNALRR185 pKa = 11.84 TSYY188 pKa = 10.16 TSQVILSPSVFVIVRR203 pKa = 11.84 LYY205 pKa = 11.25 LLVIPASPVKK215 pKa = 10.64
Molecular weight: 24.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.355
IPC2_protein 9.677
IPC_protein 10.482
Toseland 10.657
ProMoST 10.409
Dawson 10.76
Bjellqvist 10.496
Wikipedia 10.979
Rodwell 10.965
Grimsley 10.818
Solomon 10.877
Lehninger 10.847
Nozaki 10.687
DTASelect 10.482
Thurlkill 10.672
EMBOSS 11.067
Sillero 10.701
Patrickios 10.687
IPC_peptide 10.877
IPC2_peptide 9.838
IPC2.peptide.svr19 8.492
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
59
0
59
11987
34
852
203.2
22.47
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.869 ± 0.617
0.934 ± 0.142
5.998 ± 0.247
6.749 ± 0.433
3.837 ± 0.167
7.667 ± 0.353
1.593 ± 0.161
4.738 ± 0.221
5.648 ± 0.304
7.633 ± 0.33
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.561 ± 0.152
4.563 ± 0.3
3.921 ± 0.243
3.904 ± 0.254
5.623 ± 0.319
6.123 ± 0.342
6.298 ± 0.351
7.567 ± 0.331
1.418 ± 0.151
3.354 ± 0.195
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here