Amino acid dipepetide frequency for Parvularcula sp. SM1705

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	14.864AlaAla: 14.864 ± 0.17	1.019AlaCys: 1.019 ± 0.038	6.885AlaAsp: 6.885 ± 0.077	8.292AlaGlu: 8.292 ± 0.114	4.352AlaPhe: 4.352 ± 0.066	10.045AlaGly: 10.045 ± 0.124	1.993AlaHis: 1.993 ± 0.049	5.802AlaIle: 5.802 ± 0.08	4.042AlaLys: 4.042 ± 0.086	11.439AlaLeu: 11.439 ± 0.133	3.14AlaMet: 3.14 ± 0.061	2.719AlaAsn: 2.719 ± 0.067	5.315AlaPro: 5.315 ± 0.087	3.39AlaGln: 3.39 ± 0.063	7.317AlaArg: 7.317 ± 0.099	6.114AlaSer: 6.114 ± 0.088	5.079AlaThr: 5.079 ± 0.077	7.356AlaVal: 7.356 ± 0.091	1.182AlaTrp: 1.182 ± 0.034	2.714AlaTyr: 2.714 ± 0.058	0.0AlaXaa: 0.0 ± 0.0
Cys	0.853CysAla: 0.853 ± 0.025	0.086CysCys: 0.086 ± 0.01	0.476CysAsp: 0.476 ± 0.024	0.476CysGlu: 0.476 ± 0.024	0.302CysPhe: 0.302 ± 0.017	0.844CysGly: 0.844 ± 0.033	0.222CysHis: 0.222 ± 0.02	0.314CysIle: 0.314 ± 0.019	0.177CysLys: 0.177 ± 0.015	0.777CysLeu: 0.777 ± 0.03	0.133CysMet: 0.133 ± 0.012	0.172CysAsn: 0.172 ± 0.014	0.452CysPro: 0.452 ± 0.024	0.225CysGln: 0.225 ± 0.016	0.518CysArg: 0.518 ± 0.026	0.446CysSer: 0.446 ± 0.022	0.407CysThr: 0.407 ± 0.02	0.559CysVal: 0.559 ± 0.024	0.09CysTrp: 0.09 ± 0.01	0.183CysTyr: 0.183 ± 0.014	0.0CysXaa: 0.0 ± 0.0
Asp	6.643AspAla: 6.643 ± 0.087	0.479AspCys: 0.479 ± 0.025	4.506AspAsp: 4.506 ± 0.126	4.934AspGlu: 4.934 ± 0.086	2.55AspPhe: 2.55 ± 0.06	6.288AspGly: 6.288 ± 0.119	1.305AspHis: 1.305 ± 0.044	3.73AspIle: 3.73 ± 0.068	1.92AspLys: 1.92 ± 0.048	5.962AspLeu: 5.962 ± 0.085	1.567AspMet: 1.567 ± 0.038	1.666AspAsn: 1.666 ± 0.056	3.522AspPro: 3.522 ± 0.068	1.931AspGln: 1.931 ± 0.043	4.094AspArg: 4.094 ± 0.066	2.457AspSer: 2.457 ± 0.065	3.267AspThr: 3.267 ± 0.108	4.603AspVal: 4.603 ± 0.075	1.064AspTrp: 1.064 ± 0.036	1.832AspTyr: 1.832 ± 0.045	0.0AspXaa: 0.0 ± 0.0
Glu	8.533GluAla: 8.533 ± 0.137	0.368GluCys: 0.368 ± 0.022	4.568GluAsp: 4.568 ± 0.071	5.526GluGlu: 5.526 ± 0.102	2.124GluPhe: 2.124 ± 0.042	5.786GluGly: 5.786 ± 0.09	1.214GluHis: 1.214 ± 0.038	3.909GluIle: 3.909 ± 0.065	3.06GluLys: 3.06 ± 0.065	5.802GluLeu: 5.802 ± 0.086	1.962GluMet: 1.962 ± 0.045	2.18GluAsn: 2.18 ± 0.048	2.768GluPro: 2.768 ± 0.062	2.22GluGln: 2.22 ± 0.046	4.604GluArg: 4.604 ± 0.073	2.583GluSer: 2.583 ± 0.053	4.303GluThr: 4.303 ± 0.061	4.3GluVal: 4.3 ± 0.065	0.859GluTrp: 0.859 ± 0.03	1.348GluTyr: 1.348 ± 0.04	0.0GluXaa: 0.0 ± 0.0
Phe	4.373PheAla: 4.373 ± 0.068	0.378PheCys: 0.378 ± 0.02	2.928PheAsp: 2.928 ± 0.064	2.654PheGlu: 2.654 ± 0.047	1.59PhePhe: 1.59 ± 0.039	4.018PheGly: 4.018 ± 0.075	0.741PheHis: 0.741 ± 0.029	2.031PheIle: 2.031 ± 0.057	0.982PheLys: 0.982 ± 0.035	3.787PheLeu: 3.787 ± 0.07	0.864PheMet: 0.864 ± 0.029	1.218PheAsn: 1.218 ± 0.039	1.595PhePro: 1.595 ± 0.04	0.938PheGln: 0.938 ± 0.029	2.265PheArg: 2.265 ± 0.045	2.608PheSer: 2.608 ± 0.056	2.373PheThr: 2.373 ± 0.052	2.709PheVal: 2.709 ± 0.06	0.606PheTrp: 0.606 ± 0.026	1.036PheTyr: 1.036 ± 0.037	0.0PheXaa: 0.0 ± 0.0
Gly	9.23GlyAla: 9.23 ± 0.123	0.699GlyCys: 0.699 ± 0.028	5.473GlyAsp: 5.473 ± 0.14	6.011GlyGlu: 6.011 ± 0.1	3.822GlyPhe: 3.822 ± 0.077	7.892GlyGly: 7.892 ± 0.151	1.852GlyHis: 1.852 ± 0.045	4.612GlyIle: 4.612 ± 0.076	3.242GlyLys: 3.242 ± 0.06	8.613GlyLeu: 8.613 ± 0.109	2.418GlyMet: 2.418 ± 0.053	2.618GlyAsn: 2.618 ± 0.1	3.553GlyPro: 3.553 ± 0.064	2.913GlyGln: 2.913 ± 0.052	5.447GlyArg: 5.447 ± 0.081	4.529GlySer: 4.529 ± 0.093	4.805GlyThr: 4.805 ± 0.094	5.992GlyVal: 5.992 ± 0.092	1.36GlyTrp: 1.36 ± 0.04	2.416GlyTyr: 2.416 ± 0.045	0.0GlyXaa: 0.0 ± 0.0
His	1.916HisAla: 1.916 ± 0.042	0.192HisCys: 0.192 ± 0.014	1.214HisAsp: 1.214 ± 0.036	1.31HisGlu: 1.31 ± 0.041	0.828HisPhe: 0.828 ± 0.027	1.792HisGly: 1.792 ± 0.047	0.563HisHis: 0.563 ± 0.027	0.957HisIle: 0.957 ± 0.029	0.577HisLys: 0.577 ± 0.027	2.034HisLeu: 2.034 ± 0.051	0.461HisMet: 0.461 ± 0.017	0.453HisAsn: 0.453 ± 0.021	1.246HisPro: 1.246 ± 0.034	0.614HisGln: 0.614 ± 0.025	1.203HisArg: 1.203 ± 0.036	0.872HisSer: 0.872 ± 0.032	0.792HisThr: 0.792 ± 0.026	1.294HisVal: 1.294 ± 0.034	0.262HisTrp: 0.262 ± 0.016	0.594HisTyr: 0.594 ± 0.025	0.0HisXaa: 0.0 ± 0.0
Ile	6.637IleAla: 6.637 ± 0.088	0.502IleCys: 0.502 ± 0.023	4.026IleAsp: 4.026 ± 0.068	4.122IleGlu: 4.122 ± 0.068	2.121IlePhe: 2.121 ± 0.058	5.029IleGly: 5.029 ± 0.083	0.95IleHis: 0.95 ± 0.034	2.862IleIle: 2.862 ± 0.054	1.546IleLys: 1.546 ± 0.038	4.831IleLeu: 4.831 ± 0.077	1.028IleMet: 1.028 ± 0.031	1.562IleAsn: 1.562 ± 0.053	2.451IlePro: 2.451 ± 0.052	1.233IleGln: 1.233 ± 0.034	3.423IleArg: 3.423 ± 0.06	3.57IleSer: 3.57 ± 0.064	3.066IleThr: 3.066 ± 0.072	3.853IleVal: 3.853 ± 0.07	0.649IleTrp: 0.649 ± 0.027	1.3IleTyr: 1.3 ± 0.037	0.0IleXaa: 0.0 ± 0.0
Lys	4.229LysAla: 4.229 ± 0.078	0.162LysCys: 0.162 ± 0.012	2.157LysAsp: 2.157 ± 0.049	2.216LysGlu: 2.216 ± 0.049	1.152LysPhe: 1.152 ± 0.037	2.878LysGly: 2.878 ± 0.062	0.58LysHis: 0.58 ± 0.024	1.825LysIle: 1.825 ± 0.048	1.761LysLys: 1.761 ± 0.065	3.177LysLeu: 3.177 ± 0.058	0.863LysMet: 0.863 ± 0.031	0.961LysAsn: 0.961 ± 0.033	1.894LysPro: 1.894 ± 0.043	0.906LysGln: 0.906 ± 0.032	2.301LysArg: 2.301 ± 0.062	2.168LysSer: 2.168 ± 0.054	2.17LysThr: 2.17 ± 0.051	2.263LysVal: 2.263 ± 0.057	0.445LysTrp: 0.445 ± 0.021	0.738LysTyr: 0.738 ± 0.03	0.0LysXaa: 0.0 ± 0.0
Leu	11.068LeuAla: 11.068 ± 0.125	0.851LeuCys: 0.851 ± 0.027	5.945LeuAsp: 5.945 ± 0.082	5.751LeuGlu: 5.751 ± 0.078	3.983LeuPhe: 3.983 ± 0.078	7.971LeuGly: 7.971 ± 0.101	1.693LeuHis: 1.693 ± 0.04	5.698LeuIle: 5.698 ± 0.09	3.681LeuLys: 3.681 ± 0.072	8.759LeuLeu: 8.759 ± 0.129	2.33LeuMet: 2.33 ± 0.051	2.822LeuAsn: 2.822 ± 0.056	5.223LeuPro: 5.223 ± 0.076	2.341LeuGln: 2.341 ± 0.05	5.853LeuArg: 5.853 ± 0.093	6.984LeuSer: 6.984 ± 0.093	6.147LeuThr: 6.147 ± 0.085	5.953LeuVal: 5.953 ± 0.088	1.052LeuTrp: 1.052 ± 0.037	2.299LeuTyr: 2.299 ± 0.051	0.0LeuXaa: 0.0 ± 0.0
Met	3.107MetAla: 3.107 ± 0.053	0.145MetCys: 0.145 ± 0.013	1.255MetAsp: 1.255 ± 0.044	1.332MetGlu: 1.332 ± 0.042	0.784MetPhe: 0.784 ± 0.029	1.864MetGly: 1.864 ± 0.044	0.381MetHis: 0.381 ± 0.023	1.428MetIle: 1.428 ± 0.031	1.096MetLys: 1.096 ± 0.034	2.259MetLeu: 2.259 ± 0.053	0.801MetMet: 0.801 ± 0.03	0.814MetAsn: 0.814 ± 0.027	1.467MetPro: 1.467 ± 0.038	0.727MetGln: 0.727 ± 0.028	1.654MetArg: 1.654 ± 0.043	1.879MetSer: 1.879 ± 0.04	2.133MetThr: 2.133 ± 0.048	1.568MetVal: 1.568 ± 0.04	0.228MetTrp: 0.228 ± 0.016	0.303MetTyr: 0.303 ± 0.018	0.0MetXaa: 0.0 ± 0.0
Asn	3.062AsnAla: 3.062 ± 0.062	0.22AsnCys: 0.22 ± 0.016	1.897AsnAsp: 1.897 ± 0.094	1.603AsnGlu: 1.603 ± 0.044	1.168AsnPhe: 1.168 ± 0.043	2.801AsnGly: 2.801 ± 0.113	0.495AsnHis: 0.495 ± 0.022	1.584AsnIle: 1.584 ± 0.049	0.764AsnLys: 0.764 ± 0.031	2.535AsnLeu: 2.535 ± 0.06	0.723AsnMet: 0.723 ± 0.027	0.83AsnAsn: 0.83 ± 0.037	1.849AsnPro: 1.849 ± 0.041	0.839AsnGln: 0.839 ± 0.035	1.739AsnArg: 1.739 ± 0.043	1.456AsnSer: 1.456 ± 0.045	1.346AsnThr: 1.346 ± 0.037	2.102AsnVal: 2.102 ± 0.059	0.478AsnTrp: 0.478 ± 0.022	0.802AsnTyr: 0.802 ± 0.029	0.0AsnXaa: 0.0 ± 0.0
Pro	5.471ProAla: 5.471 ± 0.085	0.33ProCys: 0.33 ± 0.02	3.807ProAsp: 3.807 ± 0.069	4.12ProGlu: 4.12 ± 0.073	2.117ProPhe: 2.117 ± 0.048	4.276ProGly: 4.276 ± 0.069	0.97ProHis: 0.97 ± 0.029	2.397ProIle: 2.397 ± 0.051	1.776ProLys: 1.776 ± 0.042	4.484ProLeu: 4.484 ± 0.07	1.167ProMet: 1.167 ± 0.037	1.449ProAsn: 1.449 ± 0.044	2.295ProPro: 2.295 ± 0.063	1.649ProGln: 1.649 ± 0.046	2.535ProArg: 2.535 ± 0.057	2.913ProSer: 2.913 ± 0.06	2.273ProThr: 2.273 ± 0.048	3.941ProVal: 3.941 ± 0.061	0.535ProTrp: 0.535 ± 0.023	1.287ProTyr: 1.287 ± 0.037	0.0ProXaa: 0.0 ± 0.0
Gln	3.213GlnAla: 3.213 ± 0.066	0.205GlnCys: 0.205 ± 0.015	1.647GlnAsp: 1.647 ± 0.042	1.672GlnGlu: 1.672 ± 0.044	1.19GlnPhe: 1.19 ± 0.037	2.381GlnGly: 2.381 ± 0.051	0.57GlnHis: 0.57 ± 0.026	1.881GlnIle: 1.881 ± 0.044	1.149GlnLys: 1.149 ± 0.036	2.837GlnLeu: 2.837 ± 0.052	0.898GlnMet: 0.898 ± 0.029	0.932GlnAsn: 0.932 ± 0.031	1.487GlnPro: 1.487 ± 0.035	1.019GlnGln: 1.019 ± 0.034	1.959GlnArg: 1.959 ± 0.048	1.896GlnSer: 1.896 ± 0.041	1.773GlnThr: 1.773 ± 0.04	1.964GlnVal: 1.964 ± 0.045	0.355GlnTrp: 0.355 ± 0.02	0.739GlnTyr: 0.739 ± 0.029	0.0GlnXaa: 0.0 ± 0.0
Arg	6.665ArgAla: 6.665 ± 0.098	0.386ArgCys: 0.386 ± 0.02	3.798ArgAsp: 3.798 ± 0.059	4.468ArgGlu: 4.468 ± 0.076	2.567ArgPhe: 2.567 ± 0.055	4.369ArgGly: 4.369 ± 0.078	1.402ArgHis: 1.402 ± 0.037	3.776ArgIle: 3.776 ± 0.061	2.303ArgLys: 2.303 ± 0.051	7.002ArgLeu: 7.002 ± 0.104	1.671ArgMet: 1.671 ± 0.05	1.705ArgAsn: 1.705 ± 0.045	3.045ArgPro: 3.045 ± 0.064	2.479ArgGln: 2.479 ± 0.053	4.745ArgArg: 4.745 ± 0.081	3.183ArgSer: 3.183 ± 0.056	3.209ArgThr: 3.209 ± 0.059	3.96ArgVal: 3.96 ± 0.066	0.789ArgTrp: 0.789 ± 0.029	1.838ArgTyr: 1.838 ± 0.05	0.0ArgXaa: 0.0 ± 0.0
Ser	6.175SerAla: 6.175 ± 0.097	0.414SerCys: 0.414 ± 0.017	3.532SerAsp: 3.532 ± 0.073	3.398SerGlu: 3.398 ± 0.061	2.588SerPhe: 2.588 ± 0.058	5.582SerGly: 5.582 ± 0.09	1.126SerHis: 1.126 ± 0.033	2.959SerIle: 2.959 ± 0.064	1.682SerLys: 1.682 ± 0.044	5.769SerLeu: 5.769 ± 0.078	1.438SerMet: 1.438 ± 0.044	1.548SerAsn: 1.548 ± 0.05	3.008SerPro: 3.008 ± 0.057	1.672SerGln: 1.672 ± 0.04	3.577SerArg: 3.577 ± 0.058	3.398SerSer: 3.398 ± 0.074	2.87SerThr: 2.87 ± 0.064	3.992SerVal: 3.992 ± 0.079	0.793SerTrp: 0.793 ± 0.028	1.478SerTyr: 1.478 ± 0.04	0.0SerXaa: 0.0 ± 0.0
Thr	6.41ThrAla: 6.41 ± 0.092	0.394ThrCys: 0.394 ± 0.022	3.792ThrAsp: 3.792 ± 0.086	3.49ThrGlu: 3.49 ± 0.059	2.126ThrPhe: 2.126 ± 0.056	5.446ThrGly: 5.446 ± 0.087	1.027ThrHis: 1.027 ± 0.031	2.994ThrIle: 2.994 ± 0.063	1.707ThrLys: 1.707 ± 0.047	5.738ThrLeu: 5.738 ± 0.076	1.138ThrMet: 1.138 ± 0.032	1.499ThrAsn: 1.499 ± 0.043	3.458ThrPro: 3.458 ± 0.055	1.493ThrGln: 1.493 ± 0.039	3.07ThrArg: 3.07 ± 0.06	3.153ThrSer: 3.153 ± 0.06	2.85ThrThr: 2.85 ± 0.073	4.172ThrVal: 4.172 ± 0.082	0.481ThrTrp: 0.481 ± 0.024	1.443ThrTyr: 1.443 ± 0.043	0.0ThrXaa: 0.0 ± 0.0
Val	6.8ValAla: 6.8 ± 0.097	0.542ValCys: 0.542 ± 0.024	3.841ValAsp: 3.841 ± 0.069	4.33ValGlu: 4.33 ± 0.069	2.75ValPhe: 2.75 ± 0.057	4.906ValGly: 4.906 ± 0.075	1.252ValHis: 1.252 ± 0.036	4.261ValIle: 4.261 ± 0.074	2.265ValLys: 2.265 ± 0.055	6.797ValLeu: 6.797 ± 0.093	1.836ValMet: 1.836 ± 0.047	2.007ValAsn: 2.007 ± 0.057	3.455ValPro: 3.455 ± 0.061	1.741ValGln: 1.741 ± 0.039	4.214ValArg: 4.214 ± 0.06	4.592ValSer: 4.592 ± 0.079	4.981ValThr: 4.981 ± 0.093	4.52ValVal: 4.52 ± 0.079	0.869ValTrp: 0.869 ± 0.029	1.568ValTyr: 1.568 ± 0.041	0.0ValXaa: 0.0 ± 0.0
Trp	1.209TrpAla: 1.209 ± 0.038	0.1TrpCys: 0.1 ± 0.012	0.681TrpAsp: 0.681 ± 0.029	0.691TrpGlu: 0.691 ± 0.028	0.53TrpPhe: 0.53 ± 0.023	0.942TrpGly: 0.942 ± 0.034	0.301TrpHis: 0.301 ± 0.017	0.635TrpIle: 0.635 ± 0.027	0.409TrpLys: 0.409 ± 0.021	1.356TrpLeu: 1.356 ± 0.041	0.309TrpMet: 0.309 ± 0.02	0.393TrpAsn: 0.393 ± 0.021	0.643TrpPro: 0.643 ± 0.029	0.569TrpGln: 0.569 ± 0.026	1.052TrpArg: 1.052 ± 0.034	0.843TrpSer: 0.843 ± 0.032	0.738TrpThr: 0.738 ± 0.029	0.779TrpVal: 0.779 ± 0.027	0.224TrpTrp: 0.224 ± 0.017	0.294TrpTyr: 0.294 ± 0.019	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.599TyrAla: 2.599 ± 0.058	0.24TyrCys: 0.24 ± 0.014	1.888TyrAsp: 1.888 ± 0.046	1.622TyrGlu: 1.622 ± 0.05	1.053TyrPhe: 1.053 ± 0.038	2.39TyrGly: 2.39 ± 0.054	0.536TyrHis: 0.536 ± 0.024	1.105TyrIle: 1.105 ± 0.038	0.708TyrLys: 0.708 ± 0.029	2.442TyrLeu: 2.442 ± 0.05	0.479TyrMet: 0.479 ± 0.022	0.744TyrAsn: 0.744 ± 0.03	1.064TyrPro: 1.064 ± 0.033	0.87TyrGln: 0.87 ± 0.031	1.79TyrArg: 1.79 ± 0.043	1.358TyrSer: 1.358 ± 0.043	1.299TyrThr: 1.299 ± 0.04	1.648TyrVal: 1.648 ± 0.042	0.375TyrTrp: 0.375 ± 0.019	0.756TyrTyr: 0.756 ± 0.031	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3100 proteins (1024218 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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