Molossus molossus circovirus 1
Average proteome isoelectric point is 5.58
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2I2MP62|A0A2I2MP62_9CIRC ATP-dependent helicase Rep OS=Molossus molossus circovirus 1 OX=1959842 GN=Rep PE=3 SV=1
MM1 pKa = 7.17 QARR4 pKa = 11.84 AYY6 pKa = 10.61 CFTLNNPTEE15 pKa = 4.18 LLDD18 pKa = 5.06 LEE20 pKa = 5.18 DD21 pKa = 3.29 GHH23 pKa = 7.6 VRR25 pKa = 11.84 YY26 pKa = 9.79 AIYY29 pKa = 10.29 QEE31 pKa = 4.56 EE32 pKa = 4.31 IGDD35 pKa = 3.51 NGTNHH40 pKa = 5.68 FQGYY44 pKa = 9.77 IEE46 pKa = 4.43 FDD48 pKa = 3.01 RR49 pKa = 11.84 PTRR52 pKa = 11.84 LGYY55 pKa = 9.86 CKK57 pKa = 10.22 RR58 pKa = 11.84 LIPGAHH64 pKa = 5.94 FEE66 pKa = 4.09 QRR68 pKa = 11.84 RR69 pKa = 11.84 GTRR72 pKa = 11.84 EE73 pKa = 3.43 EE74 pKa = 3.57 ARR76 pKa = 11.84 EE77 pKa = 3.98 YY78 pKa = 10.47 CRR80 pKa = 11.84 KK81 pKa = 9.25 PDD83 pKa = 3.44 TRR85 pKa = 11.84 VGEE88 pKa = 4.23 VFEE91 pKa = 4.37 TGVWNPTGQGNRR103 pKa = 11.84 TDD105 pKa = 3.88 YY106 pKa = 11.57 DD107 pKa = 3.56 GLKK110 pKa = 10.21 QLLVDD115 pKa = 4.32 GATTRR120 pKa = 11.84 EE121 pKa = 4.32 VLDD124 pKa = 3.72 QMPSMFFRR132 pKa = 11.84 YY133 pKa = 9.45 HH134 pKa = 5.3 SAIGKK139 pKa = 8.97 AQLLLVRR146 pKa = 11.84 DD147 pKa = 4.28 RR148 pKa = 11.84 DD149 pKa = 3.56 WPTLVIVCYY158 pKa = 9.96 GASGTGKK165 pKa = 10.18 SRR167 pKa = 11.84 WARR170 pKa = 11.84 DD171 pKa = 3.22 YY172 pKa = 11.62 CDD174 pKa = 3.83 ASNSYY179 pKa = 8.48 WVSNTKK185 pKa = 9.54 WFDD188 pKa = 3.5 GYY190 pKa = 10.77 EE191 pKa = 4.11 SQPVVVLDD199 pKa = 5.18 DD200 pKa = 3.86 FRR202 pKa = 11.84 GWLPLHH208 pKa = 5.84 QLLRR212 pKa = 11.84 LCDD215 pKa = 3.96 RR216 pKa = 11.84 YY217 pKa = 10.41 PYY219 pKa = 8.73 TVEE222 pKa = 3.51 WKK224 pKa = 9.32 GGSRR228 pKa = 11.84 KK229 pKa = 9.06 FVAHH233 pKa = 6.3 VIIITSTRR241 pKa = 11.84 RR242 pKa = 11.84 PDD244 pKa = 2.78 EE245 pKa = 4.07 WYY247 pKa = 10.02 NWEE250 pKa = 4.94 DD251 pKa = 3.37 LHH253 pKa = 6.94 EE254 pKa = 5.3 DD255 pKa = 3.37 PVQLFRR261 pKa = 11.84 RR262 pKa = 11.84 IDD264 pKa = 3.36 QFYY267 pKa = 10.57 HH268 pKa = 6.8 FIPGDD273 pKa = 3.05 ICQRR277 pKa = 11.84 DD278 pKa = 3.5 AATFCYY284 pKa = 9.88 EE285 pKa = 3.77 RR286 pKa = 11.84 AHH288 pKa = 6.28 PEE290 pKa = 3.68 EE291 pKa = 4.44 LRR293 pKa = 11.84 LLEE296 pKa = 4.25 HH297 pKa = 6.76 HH298 pKa = 6.34 VTMM301 pKa = 5.89
Molecular weight: 35.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.583
IPC2_protein 5.601
IPC_protein 5.639
Toseland 6.008
ProMoST 5.97
Dawson 5.881
Bjellqvist 5.893
Wikipedia 5.881
Rodwell 5.855
Grimsley 6.135
Solomon 5.881
Lehninger 5.868
Nozaki 6.135
DTASelect 6.326
Thurlkill 6.326
EMBOSS 6.287
Sillero 6.237
Patrickios 3.808
IPC_peptide 5.893
IPC2_peptide 6.249
IPC2.peptide.svr19 6.171
Protein with the highest isoelectric point:
>tr|A0A2I2MP62|A0A2I2MP62_9CIRC ATP-dependent helicase Rep OS=Molossus molossus circovirus 1 OX=1959842 GN=Rep PE=3 SV=1
MM1 pKa = 7.17 QARR4 pKa = 11.84 AYY6 pKa = 10.61 CFTLNNPTEE15 pKa = 4.18 LLDD18 pKa = 5.06 LEE20 pKa = 5.18 DD21 pKa = 3.29 GHH23 pKa = 7.6 VRR25 pKa = 11.84 YY26 pKa = 9.79 AIYY29 pKa = 10.29 QEE31 pKa = 4.56 EE32 pKa = 4.31 IGDD35 pKa = 3.51 NGTNHH40 pKa = 5.68 FQGYY44 pKa = 9.77 IEE46 pKa = 4.43 FDD48 pKa = 3.01 RR49 pKa = 11.84 PTRR52 pKa = 11.84 LGYY55 pKa = 9.86 CKK57 pKa = 10.22 RR58 pKa = 11.84 LIPGAHH64 pKa = 5.94 FEE66 pKa = 4.09 QRR68 pKa = 11.84 RR69 pKa = 11.84 GTRR72 pKa = 11.84 EE73 pKa = 3.43 EE74 pKa = 3.57 ARR76 pKa = 11.84 EE77 pKa = 3.98 YY78 pKa = 10.47 CRR80 pKa = 11.84 KK81 pKa = 9.25 PDD83 pKa = 3.44 TRR85 pKa = 11.84 VGEE88 pKa = 4.23 VFEE91 pKa = 4.37 TGVWNPTGQGNRR103 pKa = 11.84 TDD105 pKa = 3.88 YY106 pKa = 11.57 DD107 pKa = 3.56 GLKK110 pKa = 10.21 QLLVDD115 pKa = 4.32 GATTRR120 pKa = 11.84 EE121 pKa = 4.32 VLDD124 pKa = 3.72 QMPSMFFRR132 pKa = 11.84 YY133 pKa = 9.45 HH134 pKa = 5.3 SAIGKK139 pKa = 8.97 AQLLLVRR146 pKa = 11.84 DD147 pKa = 4.28 RR148 pKa = 11.84 DD149 pKa = 3.56 WPTLVIVCYY158 pKa = 9.96 GASGTGKK165 pKa = 10.18 SRR167 pKa = 11.84 WARR170 pKa = 11.84 DD171 pKa = 3.22 YY172 pKa = 11.62 CDD174 pKa = 3.83 ASNSYY179 pKa = 8.48 WVSNTKK185 pKa = 9.54 WFDD188 pKa = 3.5 GYY190 pKa = 10.77 EE191 pKa = 4.11 SQPVVVLDD199 pKa = 5.18 DD200 pKa = 3.86 FRR202 pKa = 11.84 GWLPLHH208 pKa = 5.84 QLLRR212 pKa = 11.84 LCDD215 pKa = 3.96 RR216 pKa = 11.84 YY217 pKa = 10.41 PYY219 pKa = 8.73 TVEE222 pKa = 3.51 WKK224 pKa = 9.32 GGSRR228 pKa = 11.84 KK229 pKa = 9.06 FVAHH233 pKa = 6.3 VIIITSTRR241 pKa = 11.84 RR242 pKa = 11.84 PDD244 pKa = 2.78 EE245 pKa = 4.07 WYY247 pKa = 10.02 NWEE250 pKa = 4.94 DD251 pKa = 3.37 LHH253 pKa = 6.94 EE254 pKa = 5.3 DD255 pKa = 3.37 PVQLFRR261 pKa = 11.84 RR262 pKa = 11.84 IDD264 pKa = 3.36 QFYY267 pKa = 10.57 HH268 pKa = 6.8 FIPGDD273 pKa = 3.05 ICQRR277 pKa = 11.84 DD278 pKa = 3.5 AATFCYY284 pKa = 9.88 EE285 pKa = 3.77 RR286 pKa = 11.84 AHH288 pKa = 6.28 PEE290 pKa = 3.68 EE291 pKa = 4.44 LRR293 pKa = 11.84 LLEE296 pKa = 4.25 HH297 pKa = 6.76 HH298 pKa = 6.34 VTMM301 pKa = 5.89
Molecular weight: 35.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.583
IPC2_protein 5.601
IPC_protein 5.639
Toseland 6.008
ProMoST 5.97
Dawson 5.881
Bjellqvist 5.893
Wikipedia 5.881
Rodwell 5.855
Grimsley 6.135
Solomon 5.881
Lehninger 5.868
Nozaki 6.135
DTASelect 6.326
Thurlkill 6.326
EMBOSS 6.287
Sillero 6.237
Patrickios 3.808
IPC_peptide 5.893
IPC2_peptide 6.249
IPC2.peptide.svr19 6.171
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
301
301
301
301.0
35.44
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.983 ± 0.0
2.658 ± 0.0
7.641 ± 0.0
6.977 ± 0.0
4.651 ± 0.0
7.309 ± 0.0
3.654 ± 0.0
3.987 ± 0.0
2.658 ± 0.0
8.306 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.329 ± 0.0
2.99 ± 0.0
4.651 ± 0.0
4.319 ± 0.0
9.635 ± 0.0
3.322 ± 0.0
6.312 ± 0.0
5.98 ± 0.0
2.99 ± 0.0
5.648 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here