Methanococcus maripaludis (strain S2 / LL)

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Methanomada group; Methanococci; Methanococcales; Methanococcaceae; Methanococcus; Methanococcus maripaludis

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1722 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q6LX80|RFRNP_METMP RNA-free ribonuclease P OS=Methanococcus maripaludis (strain S2 / LL) OX=267377 GN=MMP1472 PE=3 SV=1
MM1 pKa = 7.53SAPSKK6 pKa = 10.75LIALFILTVLCTLTISYY23 pKa = 10.52GMVVDD28 pKa = 5.27DD29 pKa = 4.74PLIVVNSEE37 pKa = 4.26KK38 pKa = 10.35VDD40 pKa = 3.28KK41 pKa = 10.54TYY43 pKa = 11.55AEE45 pKa = 4.41LLMDD49 pKa = 4.04KK50 pKa = 10.41YY51 pKa = 9.11YY52 pKa = 9.89TKK54 pKa = 10.77RR55 pKa = 11.84SIEE58 pKa = 4.11VNSDD62 pKa = 2.45NDD64 pKa = 3.35IVVNEE69 pKa = 4.1NIIYY73 pKa = 9.39NVPALDD79 pKa = 3.51EE80 pKa = 4.67FEE82 pKa = 5.24INDD85 pKa = 3.91GKK87 pKa = 11.41GKK89 pKa = 10.41LLVEE93 pKa = 4.61FTKK96 pKa = 10.74SGEE99 pKa = 4.11SVTYY103 pKa = 10.82KK104 pKa = 10.06NFEE107 pKa = 4.04YY108 pKa = 10.78EE109 pKa = 4.27EE110 pKa = 4.63EE111 pKa = 4.22IEE113 pKa = 5.0SDD115 pKa = 3.89DD116 pKa = 4.38EE117 pKa = 5.28GDD119 pKa = 3.41EE120 pKa = 4.14VTFMGKK126 pKa = 7.51TYY128 pKa = 11.21KK129 pKa = 10.59LIEE132 pKa = 4.18YY133 pKa = 10.53DD134 pKa = 3.63EE135 pKa = 4.88DD136 pKa = 3.69EE137 pKa = 5.89KK138 pKa = 11.14IVLGNKK144 pKa = 9.37VEE146 pKa = 5.62DD147 pKa = 3.53IDD149 pKa = 3.94TTEE152 pKa = 3.89EE153 pKa = 3.73FSYY156 pKa = 10.85DD157 pKa = 2.7IYY159 pKa = 10.54TFKK162 pKa = 10.77IIGKK166 pKa = 9.36DD167 pKa = 3.17STGEE171 pKa = 3.99VLVSVYY177 pKa = 10.68EE178 pKa = 4.12GTDD181 pKa = 2.95RR182 pKa = 11.84IEE184 pKa = 4.1NVKK187 pKa = 9.8MYY189 pKa = 10.36PDD191 pKa = 3.25DD192 pKa = 3.74TYY194 pKa = 10.98FVSGSTVSIYY204 pKa = 10.45YY205 pKa = 10.13DD206 pKa = 3.53EE207 pKa = 4.52YY208 pKa = 11.59LEE210 pKa = 5.08SGDD213 pKa = 3.15TAYY216 pKa = 10.74FFFEE220 pKa = 4.71IYY222 pKa = 10.43DD223 pKa = 3.64SLEE226 pKa = 4.21FEE228 pKa = 4.88DD229 pKa = 4.88GKK231 pKa = 11.28SISGYY236 pKa = 10.55SDD238 pKa = 3.82FDD240 pKa = 4.03TEE242 pKa = 5.35IDD244 pKa = 3.68GQTITLEE251 pKa = 3.9YY252 pKa = 10.62DD253 pKa = 3.33NPKK256 pKa = 10.4SLSEE260 pKa = 4.12NFGLLNYY267 pKa = 9.63KK268 pKa = 10.21VNLVDD273 pKa = 5.12VNGEE277 pKa = 3.9DD278 pKa = 3.15LTAFFTVTQNLNYY291 pKa = 9.96EE292 pKa = 4.07YY293 pKa = 11.41DD294 pKa = 3.44MDD296 pKa = 4.37EE297 pKa = 3.88GTKK300 pKa = 10.72YY301 pKa = 10.65FGNNIFAVKK310 pKa = 10.43LEE312 pKa = 4.08NDD314 pKa = 4.53EE315 pKa = 4.21EE316 pKa = 5.05DD317 pKa = 3.71DD318 pKa = 4.12WDD320 pKa = 5.14DD321 pKa = 4.41EE322 pKa = 4.87LYY324 pKa = 10.68LYY326 pKa = 10.81KK327 pKa = 10.82NNKK330 pKa = 9.62KK331 pKa = 9.55YY332 pKa = 11.17DD333 pKa = 3.93KK334 pKa = 10.71IVDD337 pKa = 3.86YY338 pKa = 10.78QGSVQLVDD346 pKa = 3.41QDD348 pKa = 4.23EE349 pKa = 4.48LLSASSDD356 pKa = 3.84LILIGGPVSNKK367 pKa = 6.09VTEE370 pKa = 4.66SIQNSLNIEE379 pKa = 4.06VTNDD383 pKa = 3.13YY384 pKa = 10.4PGEE387 pKa = 4.26GKK389 pKa = 10.73GVIQTITNPNNAEE402 pKa = 3.98STILVLAGSDD412 pKa = 3.51RR413 pKa = 11.84EE414 pKa = 4.43GTKK417 pKa = 10.61ACVLALNQGLYY428 pKa = 10.46SGTGALTVKK437 pKa = 9.67LTGEE441 pKa = 4.39DD442 pKa = 3.56SVSVII447 pKa = 4.39

Molecular weight: 50.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q6M0Y3|Q6M0Y3_METMP Threonine synthase OS=Methanococcus maripaludis (strain S2 / LL) OX=267377 GN=thrC PE=3 SV=1
MM1 pKa = 7.77AGNKK5 pKa = 9.37PLGKK9 pKa = 10.1KK10 pKa = 9.19IRR12 pKa = 11.84LAKK15 pKa = 10.25ALKK18 pKa = 9.04QNRR21 pKa = 11.84RR22 pKa = 11.84VPMFAIARR30 pKa = 11.84TKK32 pKa = 10.89GSVKK36 pKa = 8.79QHH38 pKa = 5.52PKK40 pKa = 7.54MRR42 pKa = 11.84HH43 pKa = 3.6WRR45 pKa = 11.84RR46 pKa = 11.84KK47 pKa = 7.42NLKK50 pKa = 9.64KK51 pKa = 10.79

Molecular weight: 6.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1722

0

1722

490599

32

2076

284.9

31.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.809 ± 0.071

1.367 ± 0.04

5.602 ± 0.048

8.131 ± 0.06

4.2 ± 0.05

6.753 ± 0.049

1.501 ± 0.023

9.401 ± 0.059

8.849 ± 0.067

9.088 ± 0.064

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.623 ± 0.031

5.637 ± 0.064

3.398 ± 0.031

1.871 ± 0.024

3.096 ± 0.043

6.257 ± 0.055

4.965 ± 0.044

6.982 ± 0.052

0.65 ± 0.019

3.82 ± 0.04
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski