Bos taurus papillomavirus 17
Average proteome isoelectric point is 6.14
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1B2K203|A0A1B2K203_9PAPI Replication protein E1 OS=Bos taurus papillomavirus 17 OX=1887215 GN=E1 PE=3 SV=1
MM1 pKa = 7.59 RR2 pKa = 11.84 GEE4 pKa = 4.67 APTIPDD10 pKa = 3.38 IEE12 pKa = 5.64 LDD14 pKa = 3.78 LRR16 pKa = 11.84 DD17 pKa = 4.21 LVTAVDD23 pKa = 4.31 LQCNEE28 pKa = 4.04 TLSPDD33 pKa = 3.94 FEE35 pKa = 4.78 AVPEE39 pKa = 4.35 PPSPYY44 pKa = 10.01 KK45 pKa = 10.76 VVSSCFNCGCKK56 pKa = 10.19 LRR58 pKa = 11.84 IFVVASSGSIRR69 pKa = 11.84 NFHH72 pKa = 6.37 QLLLGNLSFLCVTCGKK88 pKa = 10.26 VVFSRR93 pKa = 3.8
Molecular weight: 10.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.013
IPC2_protein 5.308
IPC_protein 5.054
Toseland 4.952
ProMoST 5.207
Dawson 5.041
Bjellqvist 5.181
Wikipedia 4.927
Rodwell 4.952
Grimsley 4.876
Solomon 5.041
Lehninger 5.003
Nozaki 5.169
DTASelect 5.321
Thurlkill 4.991
EMBOSS 4.965
Sillero 5.232
Patrickios 3.541
IPC_peptide 5.054
IPC2_peptide 5.232
IPC2.peptide.svr19 5.267
Protein with the highest isoelectric point:
>tr|A0A1B2K202|A0A1B2K202_9PAPI Protein E7 OS=Bos taurus papillomavirus 17 OX=1887215 GN=E7 PE=3 SV=1
MM1 pKa = 7.66 TSLQDD6 pKa = 3.5 RR7 pKa = 11.84 FDD9 pKa = 4.07 AVQSALMDD17 pKa = 3.42 IYY19 pKa = 10.05 EE20 pKa = 4.34 TAEE23 pKa = 3.85 QTIEE27 pKa = 3.98 TQIKK31 pKa = 7.9 HH32 pKa = 5.23 WEE34 pKa = 4.13 LIRR37 pKa = 11.84 RR38 pKa = 11.84 EE39 pKa = 3.95 NVLLYY44 pKa = 10.08 SARR47 pKa = 11.84 QHH49 pKa = 5.6 NLSRR53 pKa = 11.84 LGFRR57 pKa = 11.84 PVPPLKK63 pKa = 10.61 VSEE66 pKa = 4.39 NKK68 pKa = 10.23 AKK70 pKa = 10.19 EE71 pKa = 4.03 AIGISLYY78 pKa = 10.68 LQSLKK83 pKa = 10.73 NSPFGTEE90 pKa = 3.41 RR91 pKa = 11.84 WTLQDD96 pKa = 3.22 TSSEE100 pKa = 4.02 NFNNSPSGTFKK111 pKa = 10.98 KK112 pKa = 10.36 NPRR115 pKa = 11.84 LATVVYY121 pKa = 10.5 DD122 pKa = 4.11 NNPMNAMEE130 pKa = 4.16 YY131 pKa = 9.44 PVWGNIYY138 pKa = 10.37 LLSDD142 pKa = 3.64 DD143 pKa = 5.43 DD144 pKa = 3.91 VWHH147 pKa = 7.04 KK148 pKa = 10.86 YY149 pKa = 9.04 VSSVDD154 pKa = 3.57 YY155 pKa = 11.34 DD156 pKa = 3.78 GVFYY160 pKa = 10.66 TDD162 pKa = 2.9 EE163 pKa = 4.81 HH164 pKa = 6.42 GNKK167 pKa = 9.4 VYY169 pKa = 10.66 YY170 pKa = 10.5 VQFADD175 pKa = 3.82 DD176 pKa = 3.74 AQMFSKK182 pKa = 10.65 LGQWEE187 pKa = 4.24 VTFANTVFSAPVTSSQSSSRR207 pKa = 11.84 PPGGPDD213 pKa = 2.8 TSQRR217 pKa = 11.84 EE218 pKa = 4.26 NTQGSSPSSSTAHH231 pKa = 6.3 RR232 pKa = 11.84 PEE234 pKa = 4.55 SQSSWRR240 pKa = 11.84 SASRR244 pKa = 11.84 GSPEE248 pKa = 3.84 TSEE251 pKa = 4.5 GRR253 pKa = 11.84 QSRR256 pKa = 11.84 NRR258 pKa = 11.84 HH259 pKa = 3.84 RR260 pKa = 11.84 HH261 pKa = 4.12 SRR263 pKa = 11.84 SRR265 pKa = 11.84 TRR267 pKa = 11.84 SRR269 pKa = 11.84 SRR271 pKa = 11.84 SRR273 pKa = 11.84 EE274 pKa = 3.87 SEE276 pKa = 4.1 PNSVRR281 pKa = 11.84 SRR283 pKa = 11.84 SYY285 pKa = 10.08 RR286 pKa = 11.84 SRR288 pKa = 11.84 SRR290 pKa = 11.84 SRR292 pKa = 11.84 EE293 pKa = 3.88 SRR295 pKa = 11.84 SSSAGGDD302 pKa = 3.16 RR303 pKa = 11.84 LSDD306 pKa = 3.29 RR307 pKa = 11.84 GRR309 pKa = 11.84 GRR311 pKa = 11.84 QRR313 pKa = 11.84 PEE315 pKa = 3.56 PGGRR319 pKa = 11.84 TPPAPWEE326 pKa = 3.81 VGQRR330 pKa = 11.84 SRR332 pKa = 11.84 TPSRR336 pKa = 11.84 KK337 pKa = 8.76 SQTRR341 pKa = 11.84 LEE343 pKa = 4.09 QLISEE348 pKa = 4.58 ARR350 pKa = 11.84 DD351 pKa = 3.29 PPIILVRR358 pKa = 11.84 GPQNTLKK365 pKa = 10.49 CWRR368 pKa = 11.84 NRR370 pKa = 11.84 SKK372 pKa = 10.87 HH373 pKa = 4.72 RR374 pKa = 11.84 WSGYY378 pKa = 6.6 FQSMSTTFNWICSTSHH394 pKa = 4.94 QRR396 pKa = 11.84 ASGSRR401 pKa = 11.84 MLISFNSVQQRR412 pKa = 11.84 EE413 pKa = 4.49 LFLKK417 pKa = 10.18 HH418 pKa = 6.34 VKK420 pKa = 9.84 FPKK423 pKa = 9.35 NTTHH427 pKa = 6.22 VFGFIDD433 pKa = 3.85 GLL435 pKa = 3.88
Molecular weight: 49.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.31
IPC2_protein 9.545
IPC_protein 10.277
Toseland 10.35
ProMoST 10.087
Dawson 10.511
Bjellqvist 10.233
Wikipedia 10.73
Rodwell 10.687
Grimsley 10.584
Solomon 10.584
Lehninger 10.54
Nozaki 10.321
DTASelect 10.233
Thurlkill 10.379
EMBOSS 10.745
Sillero 10.438
Patrickios 10.277
IPC_peptide 10.584
IPC2_peptide 9.092
IPC2.peptide.svr19 8.714
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
2328
93
609
388.0
43.85
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.026 ± 0.591
2.32 ± 1.109
5.455 ± 0.462
6.357 ± 0.332
5.198 ± 0.399
6.014 ± 0.712
1.89 ± 0.287
4.768 ± 0.654
5.069 ± 0.913
7.818 ± 0.703
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.761 ± 0.333
5.369 ± 0.3
6.1 ± 0.868
4.553 ± 0.503
6.271 ± 1.123
9.192 ± 1.45
6.186 ± 0.836
6.1 ± 0.692
1.632 ± 0.417
2.921 ± 0.239
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here