Bat mastadenovirus WIV11
Average proteome isoelectric point is 6.52
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 33 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A161DYT5|A0A161DYT5_9ADEN E4 ORF3 OS=Bat mastadenovirus WIV11 OX=1788433 PE=4 SV=1
MM1 pKa = 7.44 KK2 pKa = 10.21 KK3 pKa = 9.68 IEE5 pKa = 4.43 LVLDD9 pKa = 4.15 DD10 pKa = 4.96 SVLNLADD17 pKa = 3.46 VLLGQDD23 pKa = 3.7 HH24 pKa = 7.15 PEE26 pKa = 4.17 EE27 pKa = 4.52 EE28 pKa = 4.31 PQEE31 pKa = 3.96 LSIFSGEE38 pKa = 4.42 DD39 pKa = 3.24 PPTLHH44 pKa = 7.55 DD45 pKa = 4.74 LFDD48 pKa = 3.78 VVLGGDD54 pKa = 3.82 HH55 pKa = 7.63 PDD57 pKa = 3.47 YY58 pKa = 11.44 SPDD61 pKa = 3.29 VNLIFPTAEE70 pKa = 3.93 LEE72 pKa = 4.06 QAEE75 pKa = 4.4 AEE77 pKa = 4.21 NEE79 pKa = 4.11 EE80 pKa = 4.35 EE81 pKa = 4.42 EE82 pKa = 4.73 VRR84 pKa = 11.84 CEE86 pKa = 4.18 TPVVQVDD93 pKa = 4.12 LEE95 pKa = 4.66 CNEE98 pKa = 4.27 SMISTTPEE106 pKa = 3.82 GSVGSPEE113 pKa = 3.92 RR114 pKa = 11.84 EE115 pKa = 3.95 EE116 pKa = 4.58 PEE118 pKa = 4.22 EE119 pKa = 4.66 GPSSRR124 pKa = 11.84 WGPLEE129 pKa = 4.73 PGDD132 pKa = 4.25 SPRR135 pKa = 11.84 PCRR138 pKa = 11.84 DD139 pKa = 3.06 CTYY142 pKa = 10.79 HH143 pKa = 7.25 RR144 pKa = 11.84 VEE146 pKa = 5.27 SGDD149 pKa = 3.79 PSLKK153 pKa = 10.62 CGLCYY158 pKa = 10.27 MKK160 pKa = 9.96 DD161 pKa = 2.87 TYY163 pKa = 10.49 YY164 pKa = 10.67 QVYY167 pKa = 10.29 SPVSPATPLTTEE179 pKa = 3.91 EE180 pKa = 4.99 DD181 pKa = 4.18 VVNDD185 pKa = 3.39 QPEE188 pKa = 4.33 EE189 pKa = 4.32 VVTSAPSSPVAEE201 pKa = 4.19 EE202 pKa = 3.81 TGRR205 pKa = 11.84 GARR208 pKa = 11.84 RR209 pKa = 11.84 RR210 pKa = 11.84 PAEE213 pKa = 3.75 AAEE216 pKa = 4.35 PLDD219 pKa = 3.7 LSLPKK224 pKa = 9.94 RR225 pKa = 11.84 RR226 pKa = 11.84 RR227 pKa = 11.84 QQQ229 pKa = 2.85
Molecular weight: 25.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.217
IPC2_protein 4.139
IPC_protein 4.088
Toseland 3.923
ProMoST 4.202
Dawson 4.037
Bjellqvist 4.19
Wikipedia 3.897
Rodwell 3.923
Grimsley 3.834
Solomon 4.037
Lehninger 3.986
Nozaki 4.151
DTASelect 4.279
Thurlkill 3.935
EMBOSS 3.923
Sillero 4.202
Patrickios 3.389
IPC_peptide 4.037
IPC2_peptide 4.19
IPC2.peptide.svr19 4.104
Protein with the highest isoelectric point:
>tr|A0A163HKY0|A0A163HKY0_9ADEN Hexon protein OS=Bat mastadenovirus WIV11 OX=1788433 GN=L3 PE=2 SV=1
MM1 pKa = 7.83 AKK3 pKa = 10.26 RR4 pKa = 11.84 MVCRR8 pKa = 11.84 VRR10 pKa = 11.84 VPIRR14 pKa = 11.84 TASVRR19 pKa = 11.84 RR20 pKa = 11.84 RR21 pKa = 11.84 RR22 pKa = 11.84 RR23 pKa = 11.84 VRR25 pKa = 11.84 RR26 pKa = 11.84 RR27 pKa = 11.84 RR28 pKa = 11.84 AGTIASCYY36 pKa = 9.27 SACQRR41 pKa = 11.84 RR42 pKa = 11.84 FGVTGKK48 pKa = 10.05 RR49 pKa = 11.84 SRR51 pKa = 11.84 RR52 pKa = 11.84 GRR54 pKa = 11.84 RR55 pKa = 11.84 RR56 pKa = 11.84 RR57 pKa = 11.84 GRR59 pKa = 11.84 RR60 pKa = 11.84 IRR62 pKa = 11.84 GGFLPALVPIIAAAIGAIPGIAGVAIQASQRR93 pKa = 11.84 HH94 pKa = 4.76
Molecular weight: 10.55 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.401
IPC2_protein 10.935
IPC_protein 12.471
Toseland 12.618
ProMoST 13.13
Dawson 12.632
Bjellqvist 12.618
Wikipedia 13.1
Rodwell 12.164
Grimsley 12.661
Solomon 13.13
Lehninger 13.027
Nozaki 12.618
DTASelect 12.618
Thurlkill 12.618
EMBOSS 13.13
Sillero 12.618
Patrickios 11.886
IPC_peptide 13.13
IPC2_peptide 12.12
IPC2.peptide.svr19 9.143
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
29
4
33
12668
58
1648
383.9
43.06
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.815 ± 0.479
1.926 ± 0.27
4.721 ± 0.24
6.228 ± 0.538
3.9 ± 0.279
6.252 ± 0.447
2.25 ± 0.173
4.113 ± 0.349
4.16 ± 0.442
9.591 ± 0.48
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.029 ± 0.147
4.705 ± 0.454
6.434 ± 0.395
4.61 ± 0.278
6.876 ± 0.687
7.302 ± 0.315
5.565 ± 0.27
6.481 ± 0.314
1.366 ± 0.171
3.679 ± 0.255
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here