Sclerotinia sclerotiorum hypovirulence associated DNA virus 1

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Genomoviridae; Gemycircularvirus; Gemycircularvirus sclero1; Sclerotinia gemycircularvirus 1

Average proteome isoelectric point is 7.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C7FFR1|C7FFR1_9VIRU Replication-associated protein OS=Sclerotinia sclerotiorum hypovirulence associated DNA virus 1 OX=664785 GN=AC1 PE=3 SV=1
MM1 pKa = 7.42TFDD4 pKa = 3.38FHH6 pKa = 8.1AKK8 pKa = 9.84YY9 pKa = 10.92VLLTYY14 pKa = 9.04AQCGEE19 pKa = 3.98LDD21 pKa = 3.5AFRR24 pKa = 11.84VMDD27 pKa = 4.21KK28 pKa = 11.15LSLLGAEE35 pKa = 4.59CIIGRR40 pKa = 11.84EE41 pKa = 4.01HH42 pKa = 7.75HH43 pKa = 6.83EE44 pKa = 4.72DD45 pKa = 3.5GGTHH49 pKa = 5.81LHH51 pKa = 6.57CFAEE55 pKa = 5.16FGRR58 pKa = 11.84KK59 pKa = 8.16FRR61 pKa = 11.84SRR63 pKa = 11.84KK64 pKa = 9.78ADD66 pKa = 3.3VFDD69 pKa = 4.55VDD71 pKa = 3.74GHH73 pKa = 6.53HH74 pKa = 7.33PNITKK79 pKa = 10.39SRR81 pKa = 11.84GTPEE85 pKa = 3.56KK86 pKa = 10.7GYY88 pKa = 10.89DD89 pKa = 3.47YY90 pKa = 10.69AIKK93 pKa = 10.71DD94 pKa = 3.36GDD96 pKa = 4.38VICGGLGRR104 pKa = 11.84PSVGRR109 pKa = 11.84VGTRR113 pKa = 11.84PSDD116 pKa = 3.87SKK118 pKa = 10.61WAIITSASNRR128 pKa = 11.84DD129 pKa = 3.71EE130 pKa = 4.26FWEE133 pKa = 4.4LVHH136 pKa = 7.01EE137 pKa = 5.14LDD139 pKa = 4.19PKK141 pKa = 10.74AAATSFSQLQRR152 pKa = 11.84YY153 pKa = 8.74CDD155 pKa = 3.02WKK157 pKa = 9.32YY158 pKa = 10.58QYY160 pKa = 10.22HH161 pKa = 5.55APEE164 pKa = 4.41YY165 pKa = 8.76EE166 pKa = 4.57SPAGAHH172 pKa = 6.75FIGAEE177 pKa = 3.59LDD179 pKa = 3.47GRR181 pKa = 11.84NRR183 pKa = 11.84WLEE186 pKa = 3.56QSGIGSSEE194 pKa = 3.79EE195 pKa = 3.33RR196 pKa = 11.84RR197 pKa = 11.84GRR199 pKa = 11.84VKK201 pKa = 10.81SLVLYY206 pKa = 10.59GPSQTGKK213 pKa = 8.63TSWARR218 pKa = 11.84SLGKK222 pKa = 9.77HH223 pKa = 6.1IYY225 pKa = 10.15CVGLVSGTEE234 pKa = 4.02CLKK237 pKa = 11.26APDD240 pKa = 3.26VEE242 pKa = 4.5YY243 pKa = 10.97AVFDD247 pKa = 5.14DD248 pKa = 3.44IRR250 pKa = 11.84GGIKK254 pKa = 10.03FFHH257 pKa = 6.71SFKK260 pKa = 10.56EE261 pKa = 3.93WLGCQPHH268 pKa = 6.79LSVKK272 pKa = 9.79EE273 pKa = 3.98LYY275 pKa = 10.11RR276 pKa = 11.84EE277 pKa = 3.96PKK279 pKa = 9.33VIEE282 pKa = 3.96WGKK285 pKa = 9.65PSIWCSNADD294 pKa = 3.54PRR296 pKa = 11.84NEE298 pKa = 3.86LLQVDD303 pKa = 4.54IDD305 pKa = 3.82WMEE308 pKa = 4.05MNCTFIEE315 pKa = 3.87ITEE318 pKa = 4.18PVVEE322 pKa = 4.81FNN324 pKa = 4.55

Molecular weight: 36.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C7FFR1|C7FFR1_9VIRU Replication-associated protein OS=Sclerotinia sclerotiorum hypovirulence associated DNA virus 1 OX=664785 GN=AC1 PE=3 SV=1
MM1 pKa = 7.83AYY3 pKa = 10.26ARR5 pKa = 11.84YY6 pKa = 9.19RR7 pKa = 11.84SSRR10 pKa = 11.84FRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84PIKK18 pKa = 7.98TSRR21 pKa = 11.84RR22 pKa = 11.84GGVSKK27 pKa = 10.3PRR29 pKa = 11.84RR30 pKa = 11.84RR31 pKa = 11.84SYY33 pKa = 10.12SAKK36 pKa = 9.66KK37 pKa = 8.24RR38 pKa = 11.84TYY40 pKa = 9.61RR41 pKa = 11.84KK42 pKa = 8.9KK43 pKa = 10.36ASMSKK48 pKa = 10.34RR49 pKa = 11.84SILNTTSRR57 pKa = 11.84KK58 pKa = 9.22KK59 pKa = 10.51RR60 pKa = 11.84NGMLTFSNTTNSGASTAPAAAPVYY84 pKa = 10.39VNAVLGGTFIWCPTAMNLVAQSLISNMSSHH114 pKa = 6.65TATTCYY120 pKa = 9.61MRR122 pKa = 11.84GLSEE126 pKa = 5.06HH127 pKa = 6.59IRR129 pKa = 11.84IQTSSGIPWFHH140 pKa = 6.81RR141 pKa = 11.84RR142 pKa = 11.84VCFTWKK148 pKa = 10.48GLGPFNVMATEE159 pKa = 4.68SPTSDD164 pKa = 4.33FPWLPYY170 pKa = 9.71QDD172 pKa = 3.82SSNGVQRR179 pKa = 11.84LFFNEE184 pKa = 3.94NVNNMPQTINRR195 pKa = 11.84QRR197 pKa = 11.84NILFKK202 pKa = 10.77GAEE205 pKa = 4.22NVDD208 pKa = 3.07WTDD211 pKa = 4.24PIIAPLDD218 pKa = 3.41TGRR221 pKa = 11.84ITVKK225 pKa = 10.33FDD227 pKa = 3.36KK228 pKa = 10.0TWTMQSGNTNGIVRR242 pKa = 11.84EE243 pKa = 4.01RR244 pKa = 11.84KK245 pKa = 8.63LWHH248 pKa = 6.56PMNHH252 pKa = 5.87NLVYY256 pKa = 10.89DD257 pKa = 4.1DD258 pKa = 5.94DD259 pKa = 4.85EE260 pKa = 6.18YY261 pKa = 11.99GDD263 pKa = 4.17IEE265 pKa = 4.49SGSWFSTDD273 pKa = 2.8SKK275 pKa = 11.63AGMGDD280 pKa = 4.16YY281 pKa = 11.03YY282 pKa = 11.64VMDD285 pKa = 5.83IIQGGQGGTASDD297 pKa = 4.69LLRR300 pKa = 11.84MTANSTMYY308 pKa = 9.24WHH310 pKa = 7.07EE311 pKa = 4.05KK312 pKa = 9.5

Molecular weight: 35.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

636

312

324

318.0

36.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.289 ± 0.084

2.044 ± 0.75

5.503 ± 0.703

5.346 ± 1.926

4.403 ± 0.385

8.333 ± 0.666

2.987 ± 0.737

5.189 ± 0.042

5.503 ± 0.26

5.975 ± 0.808

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.83 ± 1.147

4.403 ± 1.612

4.403 ± 0.059

2.673 ± 0.147

7.39 ± 0.875

8.491 ± 1.001

6.447 ± 1.75

5.189 ± 0.486

2.83 ± 0.038

3.774 ± 0.05
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski