Laceyella sacchari (Thermoactinomyces thalpophilus)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Thermoactinomycetaceae; Laceyella

Average proteome isoelectric point is 6.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3145 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2I7SRQ4|A0A2I7SRQ4_LACSH Biotin carboxylase OS=Laceyella sacchari OX=37482 GN=accC PE=4 SV=1
MM1 pKa = 7.37FEE3 pKa = 4.27SIKK6 pKa = 10.38RR7 pKa = 11.84DD8 pKa = 3.21LSFIQGLLEE17 pKa = 4.24GDD19 pKa = 4.18VNHH22 pKa = 5.71QHH24 pKa = 5.62HH25 pKa = 6.24VEE27 pKa = 3.89YY28 pKa = 10.48RR29 pKa = 11.84GLSRR33 pKa = 11.84LVDD36 pKa = 3.73VVDD39 pKa = 4.6LLVEE43 pKa = 4.32SVEE46 pKa = 3.95QLEE49 pKa = 4.39RR50 pKa = 11.84RR51 pKa = 11.84LSEE54 pKa = 3.81LEE56 pKa = 4.35EE57 pKa = 4.01YY58 pKa = 11.17VEE60 pKa = 5.6TIDD63 pKa = 4.63EE64 pKa = 4.59DD65 pKa = 4.33LNEE68 pKa = 4.94LEE70 pKa = 4.7LLCYY74 pKa = 10.64DD75 pKa = 5.21DD76 pKa = 6.75DD77 pKa = 5.24EE78 pKa = 6.54EE79 pKa = 6.45DD80 pKa = 5.16DD81 pKa = 3.92EE82 pKa = 4.51WDD84 pKa = 3.46HH85 pKa = 6.59EE86 pKa = 4.45IEE88 pKa = 4.56VTCPEE93 pKa = 4.45CGEE96 pKa = 4.6DD97 pKa = 3.55VTIDD101 pKa = 3.88EE102 pKa = 5.95DD103 pKa = 5.26DD104 pKa = 4.86LDD106 pKa = 4.78DD107 pKa = 4.87EE108 pKa = 4.77NVEE111 pKa = 4.17LLCPKK116 pKa = 10.13CHH118 pKa = 5.81TVLDD122 pKa = 4.13LEE124 pKa = 5.06EE125 pKa = 5.58IDD127 pKa = 4.21EE128 pKa = 4.52EE129 pKa = 4.38DD130 pKa = 3.76VEE132 pKa = 4.82TVISEE137 pKa = 4.3DD138 pKa = 3.23

Molecular weight: 16.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2I7SWP1|A0A2I7SWP1_LACSH Cysteine--tRNA ligase OS=Laceyella sacchari OX=37482 GN=cysS PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.29QPSKK9 pKa = 9.77RR10 pKa = 11.84KK11 pKa = 9.37RR12 pKa = 11.84KK13 pKa = 8.63NVHH16 pKa = 4.9GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37NGRR28 pKa = 11.84KK29 pKa = 8.93VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.05GRR39 pKa = 11.84KK40 pKa = 8.7VLSAA44 pKa = 4.05

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3145

0

3145

918210

26

5032

292.0

32.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.217 ± 0.044

0.911 ± 0.015

4.924 ± 0.035

7.252 ± 0.048

3.968 ± 0.03

7.251 ± 0.044

2.439 ± 0.022

6.351 ± 0.043

5.73 ± 0.042

10.227 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.794 ± 0.019

3.234 ± 0.031

4.303 ± 0.03

4.252 ± 0.03

5.688 ± 0.041

5.237 ± 0.032

5.122 ± 0.034

7.57 ± 0.035

1.341 ± 0.022

3.189 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski