Klebsiella phage ST899-OXA48phi17.1
Average proteome isoelectric point is 6.57
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 51 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A482JAC8|A0A482JAC8_9CAUD Uncharacterized protein OS=Klebsiella phage ST899-OXA48phi17.1 OX=2555917 PE=4 SV=1
MM1 pKa = 7.39 NNVDD5 pKa = 3.07 WTIYY9 pKa = 8.99 AQYY12 pKa = 11.17 VNSTSLRR19 pKa = 11.84 SLIDD23 pKa = 3.21 TFNASVAPEE32 pKa = 3.82 DD33 pKa = 4.41 WIDD36 pKa = 3.37 TFYY39 pKa = 11.7 DD40 pKa = 3.51 LVFNIEE46 pKa = 3.93 TCGDD50 pKa = 3.75 YY51 pKa = 11.65 GLMCWGKK58 pKa = 9.3 IVDD61 pKa = 3.96 VEE63 pKa = 4.28 RR64 pKa = 11.84 LLTVTPSQQFLGFGEE79 pKa = 4.37 ATSTPAEE86 pKa = 4.21 LTDD89 pKa = 3.85 PQPFNQAPFYY99 pKa = 10.9 TGVQDD104 pKa = 3.58 TNTVVLTNDD113 pKa = 3.87 AYY115 pKa = 11.01 RR116 pKa = 11.84 KK117 pKa = 10.28 LIMCKK122 pKa = 10.34 AMANISDD129 pKa = 3.97 CTVPVMNRR137 pKa = 11.84 MLMYY141 pKa = 10.06 MFGASGRR148 pKa = 11.84 AYY150 pKa = 10.78 VRR152 pKa = 11.84 DD153 pKa = 4.24 DD154 pKa = 3.84 GNHH157 pKa = 4.05 VMSYY161 pKa = 10.26 VFEE164 pKa = 4.34 FQLSDD169 pKa = 3.61 SEE171 pKa = 4.3 LAIVQSSGALPSPPGVKK188 pKa = 10.25 VNIVQEE194 pKa = 4.31 VV195 pKa = 3.07
Molecular weight: 21.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.883
IPC2_protein 4.101
IPC_protein 4.062
Toseland 3.859
ProMoST 4.228
Dawson 4.05
Bjellqvist 4.202
Wikipedia 3.986
Rodwell 3.897
Grimsley 3.77
Solomon 4.05
Lehninger 3.999
Nozaki 4.164
DTASelect 4.406
Thurlkill 3.91
EMBOSS 3.999
Sillero 4.19
Patrickios 0.782
IPC_peptide 4.037
IPC2_peptide 4.164
IPC2.peptide.svr19 4.049
Protein with the highest isoelectric point:
>tr|A0A482J721|A0A482J721_9CAUD Uncharacterized protein OS=Klebsiella phage ST899-OXA48phi17.1 OX=2555917 PE=4 SV=1
MM1 pKa = 7.16 SRR3 pKa = 11.84 DD4 pKa = 3.47 VIEE7 pKa = 5.67 RR8 pKa = 11.84 ILDD11 pKa = 3.47 RR12 pKa = 11.84 WQKK15 pKa = 10.8 LRR17 pKa = 11.84 LCRR20 pKa = 11.84 HH21 pKa = 6.55 RR22 pKa = 11.84 GTVLVDD28 pKa = 2.9 YY29 pKa = 10.57 RR30 pKa = 11.84 ILKK33 pKa = 9.69 NFVRR37 pKa = 11.84 IYY39 pKa = 9.67 QASGEE44 pKa = 4.28 KK45 pKa = 10.31 AA46 pKa = 3.03
Molecular weight: 5.6 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.343
IPC2_protein 9.677
IPC_protein 10.584
Toseland 10.643
ProMoST 10.394
Dawson 10.76
Bjellqvist 10.496
Wikipedia 10.994
Rodwell 10.906
Grimsley 10.818
Solomon 10.891
Lehninger 10.862
Nozaki 10.643
DTASelect 10.482
Thurlkill 10.657
EMBOSS 11.052
Sillero 10.687
Patrickios 10.716
IPC_peptide 10.891
IPC2_peptide 9.589
IPC2.peptide.svr19 8.523
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
51
0
51
10778
39
872
211.3
23.23
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.584 ± 0.583
0.956 ± 0.133
5.771 ± 0.283
5.4 ± 0.362
3.795 ± 0.197
7.896 ± 0.517
1.475 ± 0.144
6.031 ± 0.295
5.372 ± 0.33
7.831 ± 0.275
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.821 ± 0.178
4.676 ± 0.208
3.915 ± 0.241
4.008 ± 0.211
4.992 ± 0.387
7.487 ± 0.341
6.253 ± 0.359
6.81 ± 0.21
1.512 ± 0.146
3.414 ± 0.229
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here