Aeromonas phage 4_L372X

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 82 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5J6U3C6|A0A5J6U3C6_9CAUD Uncharacterized protein OS=Aeromonas phage 4_L372X OX=2588519 GN=4L372X_020 PE=4 SV=1
MM1 pKa = 7.79SDD3 pKa = 3.77HH4 pKa = 6.7EE5 pKa = 4.37MSDD8 pKa = 3.49VYY10 pKa = 10.9QAVSNCINTCADD22 pKa = 3.39WPDD25 pKa = 3.44MEE27 pKa = 4.79IAGHH31 pKa = 5.99CVVEE35 pKa = 4.22MLTALGFDD43 pKa = 4.35DD44 pKa = 5.86DD45 pKa = 5.59AIIDD49 pKa = 4.0ALQVASGQSKK59 pKa = 9.77EE60 pKa = 3.93ADD62 pKa = 3.45DD63 pKa = 6.49FIDD66 pKa = 4.42EE67 pKa = 4.28MIARR71 pKa = 11.84IKK73 pKa = 10.54GG74 pKa = 3.35

Molecular weight: 8.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5J6U3C1|A0A5J6U3C1_9CAUD Uncharacterized protein OS=Aeromonas phage 4_L372X OX=2588519 GN=4L372X_010 PE=4 SV=1
MM1 pKa = 7.45SKK3 pKa = 10.46SFALIADD10 pKa = 3.74IKK12 pKa = 9.91MRR14 pKa = 11.84WFAKK18 pKa = 10.21PILFALLLVKK28 pKa = 10.43LSPPTWLVKK37 pKa = 10.34RR38 pKa = 11.84LFSIKK43 pKa = 9.9VRR45 pKa = 11.84SDD47 pKa = 3.18EE48 pKa = 4.3KK49 pKa = 11.19SCC51 pKa = 4.54

Molecular weight: 5.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

82

0

82

13397

47

892

163.4

18.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.719 ± 0.583

1.418 ± 0.169

5.874 ± 0.209

7.061 ± 0.333

3.538 ± 0.201

7.173 ± 0.268

1.724 ± 0.209

6.121 ± 0.222

6.337 ± 0.307

7.128 ± 0.274

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.292 ± 0.254

4.105 ± 0.201

3.254 ± 0.203

4.09 ± 0.273

5.27 ± 0.279

6.77 ± 0.312

5.322 ± 0.342

6.852 ± 0.241

1.485 ± 0.122

3.456 ± 0.209
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski