Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (Yeast) (Eremothecium gossypii)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Eremothecium; Eremothecium gossypii

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4760 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D8FGD7|D8FGD7_ASHGO AER270C-Ap OS=Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) OX=284811 GN=AGOS_AER270CA PE=4 SV=1
MM1 pKa = 7.78SDD3 pKa = 3.2ILEE6 pKa = 4.29LDD8 pKa = 3.09IVRR11 pKa = 11.84NDD13 pKa = 3.05IGPVLEE19 pKa = 5.03EE20 pKa = 4.17YY21 pKa = 9.22FTDD24 pKa = 3.67RR25 pKa = 11.84EE26 pKa = 4.23QFVLFAQEE34 pKa = 3.18QDD36 pKa = 3.57YY37 pKa = 11.34DD38 pKa = 3.47IDD40 pKa = 3.89TCMEE44 pKa = 3.97LCDD47 pKa = 3.99TVRR50 pKa = 11.84CLNEE54 pKa = 4.56AMDD57 pKa = 4.38EE58 pKa = 4.3TDD60 pKa = 3.18SSHH63 pKa = 7.13GGRR66 pKa = 11.84NPP68 pKa = 3.15

Molecular weight: 7.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q75AK0|Q75AK0_ASHGO Polyprenol reductase OS=Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) OX=284811 GN=AGOS_ADL073W PE=3 SV=1
MM1 pKa = 7.1RR2 pKa = 11.84AKK4 pKa = 9.09WRR6 pKa = 11.84KK7 pKa = 8.87KK8 pKa = 8.73RR9 pKa = 11.84VRR11 pKa = 11.84RR12 pKa = 11.84LKK14 pKa = 9.71RR15 pKa = 11.84KK16 pKa = 8.27RR17 pKa = 11.84RR18 pKa = 11.84KK19 pKa = 9.19VRR21 pKa = 11.84ARR23 pKa = 11.84SKK25 pKa = 10.98

Molecular weight: 3.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4758

2

4760

2330700

25

4899

489.6

54.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.87 ± 0.042

1.357 ± 0.014

5.655 ± 0.025

6.579 ± 0.029

3.939 ± 0.022

5.951 ± 0.034

2.388 ± 0.014

5.04 ± 0.028

5.664 ± 0.035

10.029 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.185 ± 0.013

4.107 ± 0.021

4.863 ± 0.034

4.261 ± 0.036

5.82 ± 0.026

7.966 ± 0.041

5.44 ± 0.032

6.492 ± 0.027

1.122 ± 0.011

3.272 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski