Amino acid dipepetide frequency for Peptoclostridium acidaminophilum DSM 3953

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.104AlaAla: 7.104 ± 0.143
1.0AlaCys: 1.0 ± 0.033
4.058AlaAsp: 4.058 ± 0.071
5.003AlaGlu: 5.003 ± 0.089
3.057AlaPhe: 3.057 ± 0.067
5.956AlaGly: 5.956 ± 0.101
1.129AlaHis: 1.129 ± 0.034
6.059AlaIle: 6.059 ± 0.085
5.115AlaLys: 5.115 ± 0.079
7.195AlaLeu: 7.195 ± 0.082
2.371AlaMet: 2.371 ± 0.061
2.708AlaAsn: 2.708 ± 0.055
1.852AlaPro: 1.852 ± 0.052
2.498AlaGln: 2.498 ± 0.057
3.435AlaArg: 3.435 ± 0.072
4.653AlaSer: 4.653 ± 0.078
3.175AlaThr: 3.175 ± 0.059
6.069AlaVal: 6.069 ± 0.105
0.493AlaTrp: 0.493 ± 0.026
2.66AlaTyr: 2.66 ± 0.057
0.0AlaXaa: 0.0 ± 0.0
Cys
0.847CysAla: 0.847 ± 0.033
0.179CysCys: 0.179 ± 0.016
0.696CysAsp: 0.696 ± 0.028
0.805CysGlu: 0.805 ± 0.029
0.442CysPhe: 0.442 ± 0.021
1.245CysGly: 1.245 ± 0.042
0.237CysHis: 0.237 ± 0.017
0.926CysIle: 0.926 ± 0.035
0.802CysLys: 0.802 ± 0.035
0.776CysLeu: 0.776 ± 0.027
0.312CysMet: 0.312 ± 0.019
0.483CysAsn: 0.483 ± 0.023
0.536CysPro: 0.536 ± 0.031
0.274CysGln: 0.274 ± 0.02
0.569CysArg: 0.569 ± 0.03
0.957CysSer: 0.957 ± 0.039
0.549CysThr: 0.549 ± 0.028
0.744CysVal: 0.744 ± 0.03
0.082CysTrp: 0.082 ± 0.01
0.342CysTyr: 0.342 ± 0.02
0.0CysXaa: 0.0 ± 0.0
Asp
3.971AspAla: 3.971 ± 0.077
0.636AspCys: 0.636 ± 0.03
2.792AspAsp: 2.792 ± 0.066
5.144AspGlu: 5.144 ± 0.092
2.857AspPhe: 2.857 ± 0.059
3.861AspGly: 3.861 ± 0.071
0.66AspHis: 0.66 ± 0.029
5.926AspIle: 5.926 ± 0.091
4.05AspLys: 4.05 ± 0.075
4.027AspLeu: 4.027 ± 0.07
2.165AspMet: 2.165 ± 0.055
2.064AspAsn: 2.064 ± 0.052
1.6AspPro: 1.6 ± 0.047
0.94AspGln: 0.94 ± 0.029
2.482AspArg: 2.482 ± 0.05
3.796AspSer: 3.796 ± 0.077
2.487AspThr: 2.487 ± 0.062
4.063AspVal: 4.063 ± 0.081
0.458AspTrp: 0.458 ± 0.024
2.459AspTyr: 2.459 ± 0.053
0.0AspXaa: 0.0 ± 0.0
Glu
5.636GluAla: 5.636 ± 0.085
0.767GluCys: 0.767 ± 0.033
4.274GluAsp: 4.274 ± 0.067
6.125GluGlu: 6.125 ± 0.107
2.938GluPhe: 2.938 ± 0.059
5.134GluGly: 5.134 ± 0.089
1.28GluHis: 1.28 ± 0.045
6.516GluIle: 6.516 ± 0.093
7.184GluLys: 7.184 ± 0.112
7.687GluLeu: 7.687 ± 0.105
2.429GluMet: 2.429 ± 0.051
4.219GluAsn: 4.219 ± 0.077
1.716GluPro: 1.716 ± 0.051
2.386GluGln: 2.386 ± 0.063
3.556GluArg: 3.556 ± 0.072
4.591GluSer: 4.591 ± 0.072
3.169GluThr: 3.169 ± 0.061
4.526GluVal: 4.526 ± 0.077
0.519GluTrp: 0.519 ± 0.03
3.096GluTyr: 3.096 ± 0.069
0.0GluXaa: 0.0 ± 0.0
Phe
2.997PheAla: 2.997 ± 0.064
0.565PheCys: 0.565 ± 0.024
2.544PheAsp: 2.544 ± 0.05
3.352PheGlu: 3.352 ± 0.06
1.801PhePhe: 1.801 ± 0.049
3.022PheGly: 3.022 ± 0.059
0.58PheHis: 0.58 ± 0.023
3.27PheIle: 3.27 ± 0.068
2.937PheLys: 2.937 ± 0.068
3.293PheLeu: 3.293 ± 0.063
1.253PheMet: 1.253 ± 0.041
1.745PheAsn: 1.745 ± 0.048
1.272PhePro: 1.272 ± 0.038
0.913PheGln: 0.913 ± 0.036
1.521PheArg: 1.521 ± 0.052
3.015PheSer: 3.015 ± 0.061
1.995PheThr: 1.995 ± 0.048
2.771PheVal: 2.771 ± 0.063
0.301PheTrp: 0.301 ± 0.02
1.406PheTyr: 1.406 ± 0.044
0.0PheXaa: 0.0 ± 0.0
Gly
5.368GlyAla: 5.368 ± 0.106
1.069GlyCys: 1.069 ± 0.037
3.755GlyAsp: 3.755 ± 0.076
5.15GlyGlu: 5.15 ± 0.081
3.422GlyPhe: 3.422 ± 0.065
5.09GlyGly: 5.09 ± 0.105
1.244GlyHis: 1.244 ± 0.041
7.091GlyIle: 7.091 ± 0.101
5.671GlyLys: 5.671 ± 0.087
6.161GlyLeu: 6.161 ± 0.094
2.538GlyMet: 2.538 ± 0.063
2.91GlyAsn: 2.91 ± 0.067
1.468GlyPro: 1.468 ± 0.051
1.727GlyGln: 1.727 ± 0.05
3.413GlyArg: 3.413 ± 0.068
4.678GlySer: 4.678 ± 0.079
3.622GlyThr: 3.622 ± 0.073
5.209GlyVal: 5.209 ± 0.096
0.552GlyTrp: 0.552 ± 0.028
3.227GlyTyr: 3.227 ± 0.063
0.0GlyXaa: 0.0 ± 0.0
His
1.079HisAla: 1.079 ± 0.041
0.214HisCys: 0.214 ± 0.016
0.797HisAsp: 0.797 ± 0.033
1.189HisGlu: 1.189 ± 0.035
0.695HisPhe: 0.695 ± 0.027
1.217HisGly: 1.217 ± 0.045
0.332HisHis: 0.332 ± 0.023
1.499HisIle: 1.499 ± 0.048
0.988HisLys: 0.988 ± 0.036
1.188HisLeu: 1.188 ± 0.036
0.529HisMet: 0.529 ± 0.024
0.615HisAsn: 0.615 ± 0.03
0.788HisPro: 0.788 ± 0.033
0.395HisGln: 0.395 ± 0.022
0.781HisArg: 0.781 ± 0.03
1.109HisSer: 1.109 ± 0.036
0.75HisThr: 0.75 ± 0.032
1.011HisVal: 1.011 ± 0.035
0.11HisTrp: 0.11 ± 0.013
0.63HisTyr: 0.63 ± 0.026
0.0HisXaa: 0.0 ± 0.0
Ile
6.56IleAla: 6.56 ± 0.096
0.941IleCys: 0.941 ± 0.038
5.372IleAsp: 5.372 ± 0.084
6.817IleGlu: 6.817 ± 0.098
3.11IlePhe: 3.11 ± 0.068
5.858IleGly: 5.858 ± 0.089
1.146IleHis: 1.146 ± 0.037
6.267IleIle: 6.267 ± 0.093
6.417IleLys: 6.417 ± 0.08
6.375IleLeu: 6.375 ± 0.093
2.38IleMet: 2.38 ± 0.058
3.583IleAsn: 3.583 ± 0.066
2.964IlePro: 2.964 ± 0.053
2.032IleGln: 2.032 ± 0.057
3.666IleArg: 3.666 ± 0.068
6.228IleSer: 6.228 ± 0.088
3.911IleThr: 3.911 ± 0.068
5.946IleVal: 5.946 ± 0.088
0.483IleTrp: 0.483 ± 0.025
2.567IleTyr: 2.567 ± 0.057
0.0IleXaa: 0.0 ± 0.0
Lys
5.862LysAla: 5.862 ± 0.089
0.695LysCys: 0.695 ± 0.035
4.194LysAsp: 4.194 ± 0.078
6.009LysGlu: 6.009 ± 0.1
2.313LysPhe: 2.313 ± 0.054
5.268LysGly: 5.268 ± 0.089
1.099LysHis: 1.099 ± 0.038
5.722LysIle: 5.722 ± 0.089
6.256LysLys: 6.256 ± 0.103
6.842LysLeu: 6.842 ± 0.103
2.212LysMet: 2.212 ± 0.052
3.831LysAsn: 3.831 ± 0.067
2.131LysPro: 2.131 ± 0.053
2.17LysGln: 2.17 ± 0.049
3.685LysArg: 3.685 ± 0.073
4.9LysSer: 4.9 ± 0.078
3.511LysThr: 3.511 ± 0.066
4.475LysVal: 4.475 ± 0.076
0.587LysTrp: 0.587 ± 0.03
2.799LysTyr: 2.799 ± 0.058
0.0LysXaa: 0.0 ± 0.0
Leu
6.501LeuAla: 6.501 ± 0.093
1.008LeuCys: 1.008 ± 0.04
5.281LeuAsp: 5.281 ± 0.074
7.699LeuGlu: 7.699 ± 0.098
3.352LeuPhe: 3.352 ± 0.074
6.708LeuGly: 6.708 ± 0.1
1.351LeuHis: 1.351 ± 0.041
6.258LeuIle: 6.258 ± 0.089
6.79LeuLys: 6.79 ± 0.106
7.958LeuLeu: 7.958 ± 0.114
2.624LeuMet: 2.624 ± 0.056
3.74LeuAsn: 3.74 ± 0.074
2.928LeuPro: 2.928 ± 0.063
2.322LeuGln: 2.322 ± 0.053
4.136LeuArg: 4.136 ± 0.072
6.982LeuSer: 6.982 ± 0.096
3.889LeuThr: 3.889 ± 0.069
5.825LeuVal: 5.825 ± 0.091
0.559LeuTrp: 0.559 ± 0.027
2.627LeuTyr: 2.627 ± 0.049
0.0LeuXaa: 0.0 ± 0.0
Met
2.631MetAla: 2.631 ± 0.064
0.311MetCys: 0.311 ± 0.019
1.941MetAsp: 1.941 ± 0.045
2.375MetGlu: 2.375 ± 0.054
1.039MetPhe: 1.039 ± 0.037
2.31MetGly: 2.31 ± 0.058
0.529MetHis: 0.529 ± 0.026
2.102MetIle: 2.102 ± 0.044
2.571MetLys: 2.571 ± 0.055
3.178MetLeu: 3.178 ± 0.066
0.8MetMet: 0.8 ± 0.029
1.531MetAsn: 1.531 ± 0.043
1.184MetPro: 1.184 ± 0.039
0.948MetGln: 0.948 ± 0.034
1.203MetArg: 1.203 ± 0.04
2.127MetSer: 2.127 ± 0.045
1.346MetThr: 1.346 ± 0.039
1.826MetVal: 1.826 ± 0.049
0.181MetTrp: 0.181 ± 0.014
0.804MetTyr: 0.804 ± 0.029
0.0MetXaa: 0.0 ± 0.0
Asn
3.151AsnAla: 3.151 ± 0.056
0.493AsnCys: 0.493 ± 0.024
1.99AsnAsp: 1.99 ± 0.055
3.008AsnGlu: 3.008 ± 0.068
1.701AsnPhe: 1.701 ± 0.046
2.965AsnGly: 2.965 ± 0.061
0.731AsnHis: 0.731 ± 0.032
4.162AsnIle: 4.162 ± 0.077
2.885AsnLys: 2.885 ± 0.059
3.702AsnLeu: 3.702 ± 0.063
1.453AsnMet: 1.453 ± 0.045
1.648AsnAsn: 1.648 ± 0.049
2.103AsnPro: 2.103 ± 0.053
1.095AsnGln: 1.095 ± 0.037
1.842AsnArg: 1.842 ± 0.048
2.77AsnSer: 2.77 ± 0.067
2.083AsnThr: 2.083 ± 0.047
3.055AsnVal: 3.055 ± 0.071
0.359AsnTrp: 0.359 ± 0.022
1.512AsnTyr: 1.512 ± 0.048
0.0AsnXaa: 0.0 ± 0.0
Pro
2.319ProAla: 2.319 ± 0.057
0.335ProCys: 0.335 ± 0.021
1.89ProAsp: 1.89 ± 0.047
2.765ProGlu: 2.765 ± 0.055
1.37ProPhe: 1.37 ± 0.04
2.461ProGly: 2.461 ± 0.058
0.615ProHis: 0.615 ± 0.026
2.057ProIle: 2.057 ± 0.052
1.956ProLys: 1.956 ± 0.057
2.69ProLeu: 2.69 ± 0.062
0.788ProMet: 0.788 ± 0.03
1.172ProAsn: 1.172 ± 0.038
0.878ProPro: 0.878 ± 0.034
1.046ProGln: 1.046 ± 0.033
1.133ProArg: 1.133 ± 0.037
2.034ProSer: 2.034 ± 0.048
1.444ProThr: 1.444 ± 0.043
2.694ProVal: 2.694 ± 0.062
0.291ProTrp: 0.291 ± 0.02
1.243ProTyr: 1.243 ± 0.041
0.0ProXaa: 0.0 ± 0.0
Gln
2.026GlnAla: 2.026 ± 0.053
0.233GlnCys: 0.233 ± 0.015
1.253GlnAsp: 1.253 ± 0.036
2.112GlnGlu: 2.112 ± 0.057
0.946GlnPhe: 0.946 ± 0.033
1.878GlnGly: 1.878 ± 0.044
0.439GlnHis: 0.439 ± 0.023
2.218GlnIle: 2.218 ± 0.054
2.315GlnLys: 2.315 ± 0.051
2.512GlnLeu: 2.512 ± 0.059
0.968GlnMet: 0.968 ± 0.032
1.299GlnAsn: 1.299 ± 0.043
0.759GlnPro: 0.759 ± 0.03
0.851GlnGln: 0.851 ± 0.036
1.43GlnArg: 1.43 ± 0.052
1.705GlnSer: 1.705 ± 0.049
1.328GlnThr: 1.328 ± 0.041
1.786GlnVal: 1.786 ± 0.041
0.217GlnTrp: 0.217 ± 0.018
0.804GlnTyr: 0.804 ± 0.034
0.0GlnXaa: 0.0 ± 0.0
Arg
3.018ArgAla: 3.018 ± 0.062
0.47ArgCys: 0.47 ± 0.028
2.554ArgAsp: 2.554 ± 0.062
3.777ArgGlu: 3.777 ± 0.07
1.83ArgPhe: 1.83 ± 0.054
2.811ArgGly: 2.811 ± 0.057
0.848ArgHis: 0.848 ± 0.033
4.02ArgIle: 4.02 ± 0.076
3.267ArgLys: 3.267 ± 0.065
4.782ArgLeu: 4.782 ± 0.083
1.408ArgMet: 1.408 ± 0.044
2.031ArgAsn: 2.031 ± 0.05
1.326ArgPro: 1.326 ± 0.047
1.367ArgGln: 1.367 ± 0.044
2.233ArgArg: 2.233 ± 0.061
2.484ArgSer: 2.484 ± 0.059
1.943ArgThr: 1.943 ± 0.051
3.048ArgVal: 3.048 ± 0.066
0.379ArgTrp: 0.379 ± 0.023
1.672ArgTyr: 1.672 ± 0.039
0.0ArgXaa: 0.0 ± 0.0
Ser
4.705SerAla: 4.705 ± 0.078
0.824SerCys: 0.824 ± 0.033
3.701SerAsp: 3.701 ± 0.07
4.845SerGlu: 4.845 ± 0.078
2.945SerPhe: 2.945 ± 0.062
5.764SerGly: 5.764 ± 0.093
1.122SerHis: 1.122 ± 0.036
5.714SerIle: 5.714 ± 0.089
4.765SerLys: 4.765 ± 0.087
6.028SerLeu: 6.028 ± 0.082
2.141SerMet: 2.141 ± 0.052
2.675SerAsn: 2.675 ± 0.062
2.091SerPro: 2.091 ± 0.058
1.989SerGln: 1.989 ± 0.058
3.329SerArg: 3.329 ± 0.079
4.779SerSer: 4.779 ± 0.103
2.961SerThr: 2.961 ± 0.067
4.529SerVal: 4.529 ± 0.071
0.534SerTrp: 0.534 ± 0.027
2.343SerTyr: 2.343 ± 0.058
0.0SerXaa: 0.0 ± 0.0
Thr
3.615ThrAla: 3.615 ± 0.075
0.554ThrCys: 0.554 ± 0.028
2.516ThrAsp: 2.516 ± 0.051
2.821ThrGlu: 2.821 ± 0.056
1.775ThrPhe: 1.775 ± 0.047
4.075ThrGly: 4.075 ± 0.074
0.805ThrHis: 0.805 ± 0.033
3.676ThrIle: 3.676 ± 0.076
2.672ThrLys: 2.672 ± 0.056
4.287ThrLeu: 4.287 ± 0.075
1.225ThrMet: 1.225 ± 0.037
1.722ThrAsn: 1.722 ± 0.047
1.883ThrPro: 1.883 ± 0.05
1.256ThrGln: 1.256 ± 0.037
1.988ThrArg: 1.988 ± 0.048
2.985ThrSer: 2.985 ± 0.069
2.315ThrThr: 2.315 ± 0.067
3.605ThrVal: 3.605 ± 0.068
0.343ThrTrp: 0.343 ± 0.022
1.606ThrTyr: 1.606 ± 0.044
0.0ThrXaa: 0.0 ± 0.0
Val
5.28ValAla: 5.28 ± 0.094
0.934ValCys: 0.934 ± 0.033
4.164ValAsp: 4.164 ± 0.077
5.331ValGlu: 5.331 ± 0.081
3.004ValPhe: 3.004 ± 0.063
4.534ValGly: 4.534 ± 0.09
1.05ValHis: 1.05 ± 0.04
5.62ValIle: 5.62 ± 0.09
5.014ValLys: 5.014 ± 0.086
6.321ValLeu: 6.321 ± 0.094
2.003ValMet: 2.003 ± 0.044
2.717ValAsn: 2.717 ± 0.062
2.301ValPro: 2.301 ± 0.053
1.833ValGln: 1.833 ± 0.042
2.907ValArg: 2.907 ± 0.059
4.808ValSer: 4.808 ± 0.076
3.211ValThr: 3.211 ± 0.058
5.117ValVal: 5.117 ± 0.09
0.501ValTrp: 0.501 ± 0.025
2.378ValTyr: 2.378 ± 0.059
0.0ValXaa: 0.0 ± 0.0
Trp
0.49TrpAla: 0.49 ± 0.027
0.091TrpCys: 0.091 ± 0.01
0.437TrpAsp: 0.437 ± 0.022
0.519TrpGlu: 0.519 ± 0.025
0.316TrpPhe: 0.316 ± 0.02
0.535TrpGly: 0.535 ± 0.029
0.153TrpHis: 0.153 ± 0.013
0.579TrpIle: 0.579 ± 0.027
0.501TrpLys: 0.501 ± 0.024
0.683TrpLeu: 0.683 ± 0.032
0.255TrpMet: 0.255 ± 0.019
0.441TrpAsn: 0.441 ± 0.025
0.182TrpPro: 0.182 ± 0.017
0.22TrpGln: 0.22 ± 0.015
0.352TrpArg: 0.352 ± 0.021
0.479TrpSer: 0.479 ± 0.024
0.363TrpThr: 0.363 ± 0.021
0.402TrpVal: 0.402 ± 0.021
0.073TrpTrp: 0.073 ± 0.008
0.252TrpTyr: 0.252 ± 0.017
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.43TyrAla: 2.43 ± 0.059
0.485TyrCys: 0.485 ± 0.026
2.229TyrAsp: 2.229 ± 0.06
2.899TyrGlu: 2.899 ± 0.059
1.641TyrPhe: 1.641 ± 0.048
2.672TyrGly: 2.672 ± 0.046
0.565TyrHis: 0.565 ± 0.027
2.94TyrIle: 2.94 ± 0.055
2.404TyrLys: 2.404 ± 0.056
2.853TyrLeu: 2.853 ± 0.064
1.084TyrMet: 1.084 ± 0.035
1.574TyrAsn: 1.574 ± 0.04
1.222TyrPro: 1.222 ± 0.04
0.815TyrGln: 0.815 ± 0.028
1.609TyrArg: 1.609 ± 0.037
2.704TyrSer: 2.704 ± 0.061
1.676TyrThr: 1.676 ± 0.047
2.325TyrVal: 2.325 ± 0.053
0.299TyrTrp: 0.299 ± 0.019
1.411TyrTyr: 1.411 ± 0.044
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2843 proteins (861762 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski