Corynebacterium sp. DNF00584

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Corynebacteriaceae; Corynebacterium; unclassified Corynebacterium

Average proteome isoelectric point is 5.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2623 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A133ZFT4|A0A133ZFT4_9CORY ABC transporter ATP-binding protein OS=Corynebacterium sp. DNF00584 OX=1384076 GN=HMPREF0307_01653 PE=3 SV=1
MM1 pKa = 7.26KK2 pKa = 10.39RR3 pKa = 11.84SITLAALVLTSTLALTACSDD23 pKa = 3.69AADD26 pKa = 4.22NSDD29 pKa = 4.81DD30 pKa = 4.95ADD32 pKa = 3.99TTSTTTATAEE42 pKa = 3.98THH44 pKa = 5.31EE45 pKa = 4.35THH47 pKa = 6.78QEE49 pKa = 3.75DD50 pKa = 4.25STTADD55 pKa = 3.84EE56 pKa = 4.16EE57 pKa = 4.82HH58 pKa = 6.94GGHH61 pKa = 6.94DD62 pKa = 3.56HH63 pKa = 7.03PADD66 pKa = 4.04GGAPPAGIEE75 pKa = 4.02EE76 pKa = 4.9AEE78 pKa = 4.43DD79 pKa = 3.24PTYY82 pKa = 10.65PVGTEE87 pKa = 4.03VILTADD93 pKa = 4.44HH94 pKa = 6.28MPGMDD99 pKa = 3.64GATATISGAFDD110 pKa = 3.15TTTYY114 pKa = 10.32SVSYY118 pKa = 9.77TPTEE122 pKa = 4.03GGAPVTDD129 pKa = 3.89HH130 pKa = 6.43RR131 pKa = 11.84WVVHH135 pKa = 6.18EE136 pKa = 4.48EE137 pKa = 3.95LVDD140 pKa = 4.02PGQAPLPDD148 pKa = 4.18GASVVLDD155 pKa = 3.87AEE157 pKa = 4.78HH158 pKa = 6.21MSGMKK163 pKa = 10.11GAEE166 pKa = 3.73ATIDD170 pKa = 3.59YY171 pKa = 8.79STEE174 pKa = 3.61EE175 pKa = 3.88TVYY178 pKa = 10.38MVDD181 pKa = 2.91ITVDD185 pKa = 3.17GMTMTNHH192 pKa = 5.38KK193 pKa = 9.32WVTEE197 pKa = 4.09SEE199 pKa = 4.23IQPAEE204 pKa = 3.8

Molecular weight: 21.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A133ZAR7|A0A133ZAR7_9CORY Uncharacterized protein OS=Corynebacterium sp. DNF00584 OX=1384076 GN=HMPREF0307_02280 PE=4 SV=1
MM1 pKa = 7.69AKK3 pKa = 10.06GKK5 pKa = 8.69RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84ARR17 pKa = 11.84KK18 pKa = 8.59HH19 pKa = 4.58GFRR22 pKa = 11.84TRR24 pKa = 11.84MSTRR28 pKa = 11.84AGRR31 pKa = 11.84AIVAARR37 pKa = 11.84RR38 pKa = 11.84KK39 pKa = 9.72KK40 pKa = 10.5GRR42 pKa = 11.84AKK44 pKa = 9.67LTAA47 pKa = 4.21

Molecular weight: 5.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2623

0

2623

796831

33

2986

303.8

32.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.507 ± 0.08

0.671 ± 0.012

6.032 ± 0.044

6.302 ± 0.053

3.414 ± 0.032

8.396 ± 0.042

2.179 ± 0.02

4.858 ± 0.043

3.224 ± 0.047

9.603 ± 0.063

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.24 ± 0.022

2.749 ± 0.03

5.049 ± 0.041

3.139 ± 0.031

6.055 ± 0.046

5.555 ± 0.037

6.227 ± 0.044

8.11 ± 0.041

1.419 ± 0.024

2.271 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski