Trematomus pennellii polyomavirus 1
Average proteome isoelectric point is 5.81
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0E3JRJ3|A0A0E3JRJ3_9POLY Large T-antigen OS=Trematomus pennellii polyomavirus 1 OX=2170103 PE=4 SV=1
MM1 pKa = 6.24 QQEE4 pKa = 4.18 YY5 pKa = 8.21 TADD8 pKa = 3.36 ANGRR12 pKa = 11.84 NRR14 pKa = 11.84 NLLEE18 pKa = 4.16 TNKK21 pKa = 9.54 EE22 pKa = 3.71 VAMANSTTFTSNVYY36 pKa = 10.04 ASKK39 pKa = 10.7 LIKK42 pKa = 10.6 ALGSSVFPGATMEE55 pKa = 5.32 DD56 pKa = 4.82 NMITWNADD64 pKa = 2.42 MDD66 pKa = 4.26 ALSFQDD72 pKa = 3.41 SQGNKK77 pKa = 7.89 EE78 pKa = 3.98 DD79 pKa = 4.99 RR80 pKa = 11.84 ILLRR84 pKa = 11.84 EE85 pKa = 4.13 KK86 pKa = 11.25 VNDD89 pKa = 3.19 LWKK92 pKa = 10.52 GYY94 pKa = 8.89 QAAEE98 pKa = 3.77 AVLADD103 pKa = 4.86 FAPSDD108 pKa = 4.03 TPKK111 pKa = 10.15 MAGPVDD117 pKa = 4.35 FCADD121 pKa = 3.2 VTEE124 pKa = 5.59 FLDD127 pKa = 4.2 PDD129 pKa = 3.98 CTSASAYY136 pKa = 10.94 LFMAPASLYY145 pKa = 8.2 PQLMRR150 pKa = 11.84 IAKK153 pKa = 9.97 GYY155 pKa = 10.55 RR156 pKa = 11.84 LDD158 pKa = 4.43 DD159 pKa = 4.08 YY160 pKa = 11.45 YY161 pKa = 11.77 GVAHH165 pKa = 6.81 TDD167 pKa = 3.65 EE168 pKa = 4.56 KK169 pKa = 10.55 WGEE172 pKa = 3.59 IQYY175 pKa = 10.81 FGFKK179 pKa = 8.83 TRR181 pKa = 11.84 YY182 pKa = 8.84 RR183 pKa = 11.84 LARR186 pKa = 11.84 HH187 pKa = 4.73 NMLRR191 pKa = 11.84 KK192 pKa = 9.33 MEE194 pKa = 4.46 ASVSNKK200 pKa = 9.08 GWVKK204 pKa = 10.23 GCPVVSKK211 pKa = 10.35 KK212 pKa = 10.66 FNMCKK217 pKa = 9.92 SHH219 pKa = 7.14 LFNCAGAEE227 pKa = 4.16 VTEE230 pKa = 4.55 GAVEE234 pKa = 4.23 FDD236 pKa = 3.67 VLCFNQQTLARR247 pKa = 11.84 YY248 pKa = 9.37 AKK250 pKa = 9.59 DD251 pKa = 3.02 TGTCDD256 pKa = 3.5 PLLLMGDD263 pKa = 3.7 YY264 pKa = 9.81 MAFSGLVHH272 pKa = 7.37 ACTQCHH278 pKa = 4.6 TEE280 pKa = 3.72 RR281 pKa = 11.84 RR282 pKa = 11.84 RR283 pKa = 11.84 GGQCHH288 pKa = 4.91 ATYY291 pKa = 10.34 HH292 pKa = 6.72 KK293 pKa = 10.17 EE294 pKa = 3.85 HH295 pKa = 6.44 QGNAKK300 pKa = 10.36 AFMMVSNKK308 pKa = 9.52 KK309 pKa = 9.78 SAATNACDD317 pKa = 3.56 EE318 pKa = 4.47 SCAVLRR324 pKa = 11.84 RR325 pKa = 11.84 ASKK328 pKa = 10.37 LPRR331 pKa = 11.84 YY332 pKa = 9.77 DD333 pKa = 3.54 RR334 pKa = 11.84 LKK336 pKa = 11.29 DD337 pKa = 3.57 NMDD340 pKa = 3.0 IAMKK344 pKa = 10.44 VLEE347 pKa = 4.9 EE348 pKa = 4.06 SDD350 pKa = 3.24 ITNSQVAAAMLLSEE364 pKa = 5.78 LIPGSVCDD372 pKa = 5.66 FFDD375 pKa = 3.6 QVISALLKK383 pKa = 10.77 AEE385 pKa = 4.19 PKK387 pKa = 9.66 KK388 pKa = 10.63 QGFIFRR394 pKa = 11.84 GPYY397 pKa = 8.32 NTGKK401 pKa = 8.58 STVAAAVMSLLGGSSLNVNSPRR423 pKa = 11.84 EE424 pKa = 3.98 RR425 pKa = 11.84 LWVEE429 pKa = 4.21 FGLAMDD435 pKa = 4.52 RR436 pKa = 11.84 FAVCFEE442 pKa = 4.39 DD443 pKa = 3.75 VQGIPSPGEE452 pKa = 3.45 PCLPFGEE459 pKa = 4.93 GWSNLDD465 pKa = 3.36 HH466 pKa = 6.53 FRR468 pKa = 11.84 EE469 pKa = 4.17 YY470 pKa = 11.27 LDD472 pKa = 4.13 GLFKK476 pKa = 11.1 VGLEE480 pKa = 4.05 KK481 pKa = 10.57 KK482 pKa = 9.21 HH483 pKa = 5.37 QNKK486 pKa = 6.99 VTQNFPPWIATTNNYY501 pKa = 9.77 IIPEE505 pKa = 4.33 SVLEE509 pKa = 4.07 RR510 pKa = 11.84 AVVIPFKK517 pKa = 10.9 RR518 pKa = 11.84 MSFDD522 pKa = 3.14 VQKK525 pKa = 10.91 YY526 pKa = 9.4 IEE528 pKa = 4.71 EE529 pKa = 4.16 FDD531 pKa = 3.25 VDD533 pKa = 4.75 LRR535 pKa = 11.84 FLSSGPCLFLLWLLEE550 pKa = 4.36 DD551 pKa = 4.13 SVDD554 pKa = 5.48 DD555 pKa = 5.45 IDD557 pKa = 6.66 DD558 pKa = 4.0 EE559 pKa = 4.63 LLDD562 pKa = 3.86 RR563 pKa = 11.84 AVVLSLAAKK572 pKa = 7.55 MIWNAEE578 pKa = 3.63 MRR580 pKa = 11.84 DD581 pKa = 4.24 FIKK584 pKa = 10.73 APPPDD589 pKa = 3.97 VLTAVYY595 pKa = 9.89 VAEE598 pKa = 5.22 DD599 pKa = 3.36 GGIRR603 pKa = 11.84 CTTGPEE609 pKa = 4.08 KK610 pKa = 10.82 EE611 pKa = 4.14 NTPFTVSKK619 pKa = 9.63 VWRR622 pKa = 11.84 AIEE625 pKa = 4.08 KK626 pKa = 10.6 EE627 pKa = 4.1 EE628 pKa = 4.03 EE629 pKa = 3.92 QLPEE633 pKa = 5.1 DD634 pKa = 3.67 IHH636 pKa = 5.79 WTDD639 pKa = 3.65 SEE641 pKa = 4.39 SDD643 pKa = 3.72 SVKK646 pKa = 10.83 SVLATPLPFTPPDD659 pKa = 4.41 PGLGSSDD666 pKa = 3.8 EE667 pKa = 4.29 EE668 pKa = 4.47 EE669 pKa = 4.29 EE670 pKa = 4.35 VPPRR674 pKa = 11.84 QIPKK678 pKa = 9.77 KK679 pKa = 9.29 RR680 pKa = 11.84 KK681 pKa = 9.48 CPFIDD686 pKa = 3.89 EE687 pKa = 4.74 AATEE691 pKa = 4.22 CADD694 pKa = 4.95 DD695 pKa = 4.08 EE696 pKa = 4.63 QSDD699 pKa = 3.82 SSEE702 pKa = 4.25 PYY704 pKa = 9.54 PVPFEE709 pKa = 4.27 KK710 pKa = 10.62 KK711 pKa = 8.87 KK712 pKa = 10.8 GPRR715 pKa = 11.84 VWVVVRR721 pKa = 11.84 EE722 pKa = 4.19 DD723 pKa = 3.51 GAIGDD728 pKa = 4.5 IEE730 pKa = 4.8 NGPAPPPDD738 pKa = 3.69 EE739 pKa = 4.18 QSTSGFTDD747 pKa = 3.49 ASGTTDD753 pKa = 3.87 KK754 pKa = 11.22 DD755 pKa = 3.88 DD756 pKa = 5.08 PDD758 pKa = 4.06 VEE760 pKa = 4.61 TSS762 pKa = 3.02
Molecular weight: 84.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.759
IPC2_protein 4.749
IPC_protein 4.724
Toseland 4.571
ProMoST 4.838
Dawson 4.673
Bjellqvist 4.825
Wikipedia 4.546
Rodwell 4.571
Grimsley 4.482
Solomon 4.673
Lehninger 4.635
Nozaki 4.787
DTASelect 4.965
Thurlkill 4.571
EMBOSS 4.571
Sillero 4.838
Patrickios 3.961
IPC_peptide 4.685
IPC2_peptide 4.838
IPC2.peptide.svr19 4.806
Protein with the highest isoelectric point:
>tr|A0A0E3JE56|A0A0E3JE56_9POLY VP1 OS=Trematomus pennellii polyomavirus 1 OX=2170103 PE=3 SV=1
MM1 pKa = 7.58 GIVIGILAGVSAGIEE16 pKa = 4.29 VAVVTAAAAAAEE28 pKa = 4.72 AIGAALATAADD39 pKa = 3.46 IGLAAEE45 pKa = 4.31 VGIAAAEE52 pKa = 4.06 TAGEE56 pKa = 4.25 LFGEE60 pKa = 4.43 TAMAGDD66 pKa = 3.52 IVGFGGLIEE75 pKa = 4.87 GATEE79 pKa = 4.79 PIAAAGMGVGGALQLAGIGATVASAVGTTVAALARR114 pKa = 11.84 TRR116 pKa = 11.84 AEE118 pKa = 3.69 GDD120 pKa = 3.59 VAHH123 pKa = 6.31 VLVNAGTEE131 pKa = 3.73 IQTAVTNIDD140 pKa = 3.27 ASSTNARR147 pKa = 11.84 VPIWLTAIMGTDD159 pKa = 3.17 EE160 pKa = 4.21 VLTNLGEE167 pKa = 4.71 LEE169 pKa = 4.18 NQFSNGRR176 pKa = 11.84 LSYY179 pKa = 11.28 DD180 pKa = 3.26 FTLDD184 pKa = 2.99 QATAIAAEE192 pKa = 4.31 AEE194 pKa = 4.09 QAGRR198 pKa = 11.84 GGVHH202 pKa = 5.26 YY203 pKa = 10.64 HH204 pKa = 6.1 PVEE207 pKa = 4.65 LDD209 pKa = 3.11 TALFEE214 pKa = 5.16 KK215 pKa = 9.77 MIKK218 pKa = 10.26 HH219 pKa = 5.08 STQTTFYY226 pKa = 10.39 LPAKK230 pKa = 10.44 NGMLLSKK237 pKa = 10.45 RR238 pKa = 11.84 DD239 pKa = 3.7 TEE241 pKa = 4.3 KK242 pKa = 11.16 VINDD246 pKa = 2.52 VHH248 pKa = 6.68 ARR250 pKa = 11.84 NTGGVDD256 pKa = 3.29 AQVLGGKK263 pKa = 9.41 NPFEE267 pKa = 4.71 SWRR270 pKa = 11.84 SMASAEE276 pKa = 3.88 QDD278 pKa = 2.73 KK279 pKa = 9.77 WLKK282 pKa = 10.78 DD283 pKa = 3.27 IEE285 pKa = 4.29 KK286 pKa = 10.54 EE287 pKa = 3.75 EE288 pKa = 4.29 GKK290 pKa = 10.62 AKK292 pKa = 9.88 EE293 pKa = 3.85 RR294 pKa = 11.84 EE295 pKa = 3.91 RR296 pKa = 11.84 RR297 pKa = 11.84 LEE299 pKa = 3.75 NQLRR303 pKa = 11.84 HH304 pKa = 5.2 LTEE307 pKa = 3.5 EE308 pKa = 3.9 HH309 pKa = 6.02 RR310 pKa = 11.84 RR311 pKa = 11.84 KK312 pKa = 10.25 SRR314 pKa = 11.84 AHH316 pKa = 6.42 RR317 pKa = 11.84 LLDD320 pKa = 3.51 EE321 pKa = 4.89 RR322 pKa = 11.84 EE323 pKa = 4.12 WEE325 pKa = 4.07 NDD327 pKa = 2.79 DD328 pKa = 4.75 RR329 pKa = 11.84 LAKK332 pKa = 10.51 KK333 pKa = 10.44 LEE335 pKa = 4.08 KK336 pKa = 10.43 DD337 pKa = 3.47 LQKK340 pKa = 11.32 VKK342 pKa = 10.88 DD343 pKa = 4.2 DD344 pKa = 3.59 NDD346 pKa = 3.17 KK347 pKa = 11.25 RR348 pKa = 11.84 EE349 pKa = 3.94 QFLEE353 pKa = 4.15 DD354 pKa = 2.96 AVHH357 pKa = 6.3 YY358 pKa = 10.39 RR359 pKa = 11.84 RR360 pKa = 11.84 EE361 pKa = 4.12 DD362 pKa = 3.48 LFHH365 pKa = 6.46 SRR367 pKa = 11.84 SMAVFRR373 pKa = 11.84 GAEE376 pKa = 4.11 YY377 pKa = 10.14 PEE379 pKa = 3.81 EE380 pKa = 4.65 WIGKK384 pKa = 4.72 MTRR387 pKa = 11.84 IRR389 pKa = 11.84 DD390 pKa = 3.56 EE391 pKa = 3.89 WRR393 pKa = 11.84 HH394 pKa = 4.59 GVQAFEE400 pKa = 4.17 VVLRR404 pKa = 11.84 KK405 pKa = 9.83 HH406 pKa = 6.44 SEE408 pKa = 3.65 HH409 pKa = 6.65 PQRR412 pKa = 11.84 GDD414 pKa = 3.1 IPVSNAYY421 pKa = 9.91 HH422 pKa = 6.88 PALIRR427 pKa = 11.84 YY428 pKa = 7.48 RR429 pKa = 11.84 RR430 pKa = 11.84 RR431 pKa = 11.84 HH432 pKa = 4.56 WRR434 pKa = 11.84 LKK436 pKa = 9.09 KK437 pKa = 10.59 AKK439 pKa = 9.27 LVAVNTSDD447 pKa = 3.58 GRR449 pKa = 11.84 AHH451 pKa = 6.9 EE452 pKa = 4.73 VTLSWYY458 pKa = 8.93 TPVGEE463 pKa = 4.49 KK464 pKa = 9.96 PVPAGLDD471 pKa = 3.06 KK472 pKa = 11.18 GFKK475 pKa = 9.56 FVRR478 pKa = 11.84 EE479 pKa = 4.11 LTEE482 pKa = 3.94 SQLSVLTDD490 pKa = 3.04 YY491 pKa = 11.22 LRR493 pKa = 11.84 YY494 pKa = 8.91 HH495 pKa = 5.46 QAGFEE500 pKa = 4.09 ISGGALNIPQRR511 pKa = 11.84 LLVGALTLQTARR523 pKa = 11.84 HH524 pKa = 5.25 QSFKK528 pKa = 11.1 APRR531 pKa = 11.84 KK532 pKa = 9.54 RR533 pKa = 11.84 KK534 pKa = 9.61 AIHH537 pKa = 6.35 QDD539 pKa = 2.91 APDD542 pKa = 3.27 QGRR545 pKa = 11.84 IRR547 pKa = 11.84 SSKK550 pKa = 8.45 RR551 pKa = 11.84 RR552 pKa = 11.84 KK553 pKa = 9.62 RR554 pKa = 11.84 RR555 pKa = 11.84 TT556 pKa = 3.14
Molecular weight: 61.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.72
IPC2_protein 7.761
IPC_protein 7.907
Toseland 8.682
ProMoST 8.273
Dawson 8.843
Bjellqvist 8.434
Wikipedia 8.975
Rodwell 9.033
Grimsley 8.887
Solomon 9.033
Lehninger 8.99
Nozaki 8.521
DTASelect 8.536
Thurlkill 8.697
EMBOSS 9.033
Sillero 8.77
Patrickios 5.13
IPC_peptide 9.019
IPC2_peptide 7.322
IPC2.peptide.svr19 7.429
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
1697
55
762
424.3
46.86
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.546 ± 1.562
1.591 ± 0.737
6.305 ± 0.789
6.953 ± 0.984
3.889 ± 0.534
7.484 ± 0.788
2.121 ± 0.548
3.712 ± 0.453
5.598 ± 0.354
8.014 ± 0.174
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.005 ± 0.745
3.771 ± 0.442
5.48 ± 1.169
4.007 ± 0.836
5.303 ± 1.263
6.6 ± 1.143
5.48 ± 0.265
6.895 ± 0.107
1.65 ± 0.154
2.593 ± 0.452
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here