Paenibacillus sp. MY03

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus; unclassified Paenibacillus

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7078 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Y5JY59|A0A1Y5JY59_9BACL Aminotransferase DegT OS=Paenibacillus sp. MY03 OX=302980 GN=B1748_29955 PE=3 SV=1
MM1 pKa = 6.58QTVTYY6 pKa = 8.93PDD8 pKa = 3.4YY9 pKa = 11.84VFFCKK14 pKa = 10.38AFQEE18 pKa = 4.28WNLFDD23 pKa = 5.75FEE25 pKa = 4.44EE26 pKa = 5.46SDD28 pKa = 3.41IKK30 pKa = 10.94QEE32 pKa = 4.08PGEE35 pKa = 4.24ALSYY39 pKa = 10.52TYY41 pKa = 10.98DD42 pKa = 3.04ATFRR46 pKa = 11.84DD47 pKa = 3.5EE48 pKa = 5.64SNYY51 pKa = 8.07KK52 pKa = 8.45TNVVISFDD60 pKa = 3.33GAAITWVIADD70 pKa = 3.73GWEE73 pKa = 3.86DD74 pKa = 3.57AYY76 pKa = 10.79EE77 pKa = 4.52EE78 pKa = 4.57ISTLYY83 pKa = 11.0DD84 pKa = 3.35SMMQLKK90 pKa = 10.51ASGRR94 pKa = 11.84QLVLL98 pKa = 3.34

Molecular weight: 11.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Y5JQ02|A0A1Y5JQ02_9BACL Uncharacterized protein OS=Paenibacillus sp. MY03 OX=302980 GN=B1748_33790 PE=4 SV=1
MM1 pKa = 7.9RR2 pKa = 11.84PTFKK6 pKa = 10.84PNVSKK11 pKa = 10.73RR12 pKa = 11.84AKK14 pKa = 8.72VHH16 pKa = 5.43GFRR19 pKa = 11.84KK20 pKa = 10.04RR21 pKa = 11.84MSTKK25 pKa = 10.18NGRR28 pKa = 11.84KK29 pKa = 8.78VLAARR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.23GRR39 pKa = 11.84KK40 pKa = 8.56VLSAA44 pKa = 4.05

Molecular weight: 5.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7078

0

7078

2658388

25

3026

375.6

41.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.66 ± 0.036

0.7 ± 0.009

5.374 ± 0.02

6.767 ± 0.027

4.095 ± 0.02

7.833 ± 0.03

2.011 ± 0.014

6.343 ± 0.028

4.755 ± 0.024

9.915 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.812 ± 0.016

3.887 ± 0.022

4.144 ± 0.018

3.587 ± 0.017

5.255 ± 0.027

6.449 ± 0.021

5.366 ± 0.024

6.835 ± 0.019

1.481 ± 0.012

3.732 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski