Botrytis cinerea mitovirus 3
Average proteome isoelectric point is 8.56
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0S4GAV3|A0A0S4GAV3_9VIRU RNA dependent RNA polymerase OS=Botrytis cinerea mitovirus 3 OX=1629666 GN=RdRp PE=4 SV=1
MM1 pKa = 7.76 KK2 pKa = 9.36 ITRR5 pKa = 11.84 TYY7 pKa = 11.31 KK8 pKa = 10.6 NLNSLLKK15 pKa = 10.68 SSIVKK20 pKa = 7.38 TKK22 pKa = 10.85 KK23 pKa = 9.28 MFSIRR28 pKa = 11.84 DD29 pKa = 3.78 SISNSLSIPLRR40 pKa = 11.84 QIQYY44 pKa = 10.93 LSFGKK49 pKa = 10.05 IRR51 pKa = 11.84 GLANRR56 pKa = 11.84 ALITRR61 pKa = 11.84 DD62 pKa = 2.91 FMTFVLKK69 pKa = 10.01 MKK71 pKa = 9.87 KK72 pKa = 9.35 NHH74 pKa = 6.37 GADD77 pKa = 4.26 FTIKK81 pKa = 9.34 WLKK84 pKa = 8.89 CCYY87 pKa = 9.82 VALQKK92 pKa = 11.13 SLGKK96 pKa = 10.45 DD97 pKa = 3.02 NLEE100 pKa = 4.03 SLRR103 pKa = 11.84 DD104 pKa = 3.83 LEE106 pKa = 4.96 PNLPLPRR113 pKa = 11.84 LINGIPAIISSNDD126 pKa = 2.94 RR127 pKa = 11.84 KK128 pKa = 10.47 LIRR131 pKa = 11.84 EE132 pKa = 3.89 MDD134 pKa = 3.56 SRR136 pKa = 11.84 IIIYY140 pKa = 9.45 WSSLFSLYY148 pKa = 10.18 RR149 pKa = 11.84 VLKK152 pKa = 10.38 CSYY155 pKa = 9.35 KK156 pKa = 10.85 LKK158 pKa = 10.29 ISSITDD164 pKa = 3.46 PFKK167 pKa = 11.33 GSINEE172 pKa = 4.11 FNDD175 pKa = 3.92 IIQGPVFSIFFDD187 pKa = 3.6 RR188 pKa = 11.84 LNGFPEE194 pKa = 4.29 LVKK197 pKa = 10.73 KK198 pKa = 11.08 SNLAPSKK205 pKa = 10.62 VRR207 pKa = 11.84 LLRR210 pKa = 11.84 SSSSSNNVSWHH221 pKa = 6.44 GIITDD226 pKa = 3.07 SWNISNSKK234 pKa = 9.86 EE235 pKa = 3.69 MSSNVSNYY243 pKa = 9.54 LASIKK248 pKa = 10.72 ALGWNTMWFNSKK260 pKa = 10.03 LNEE263 pKa = 4.17 MIEE266 pKa = 4.22 LGDD269 pKa = 3.86 RR270 pKa = 11.84 LNQVGSLKK278 pKa = 9.81 TKK280 pKa = 10.44 KK281 pKa = 10.31 SLSGQFGQFSLKK293 pKa = 10.46 EE294 pKa = 3.73 EE295 pKa = 4.19 AAGKK299 pKa = 10.03 LRR301 pKa = 11.84 IFAIVDD307 pKa = 4.02 SITQSLLSPLHH318 pKa = 7.02 DD319 pKa = 4.61 FMFDD323 pKa = 3.56 LLKK326 pKa = 10.64 KK327 pKa = 10.03 IPNDD331 pKa = 3.47 GTFDD335 pKa = 3.24 QDD337 pKa = 3.69 LSVKK341 pKa = 10.13 RR342 pKa = 11.84 SQVKK346 pKa = 10.2 SLSSGKK352 pKa = 10.26 AFSFDD357 pKa = 3.28 LSAATDD363 pKa = 3.94 RR364 pKa = 11.84 LPVDD368 pKa = 3.99 LTVKK372 pKa = 10.03 ILSKK376 pKa = 10.49 IFSDD380 pKa = 4.48 EE381 pKa = 4.98 FGTSWKK387 pKa = 10.27 QLMVNRR393 pKa = 11.84 DD394 pKa = 3.51 FFFSLTNQKK403 pKa = 10.99 DD404 pKa = 3.48 YY405 pKa = 11.34 GAPEE409 pKa = 3.99 SLRR412 pKa = 11.84 YY413 pKa = 9.59 SVGQPMGALSSWPALALTHH432 pKa = 6.47 HH433 pKa = 7.57 WILQYY438 pKa = 10.7 CSNILGRR445 pKa = 11.84 TGWEE449 pKa = 3.65 EE450 pKa = 3.73 NYY452 pKa = 10.47 EE453 pKa = 4.03 ILGDD457 pKa = 3.9 DD458 pKa = 4.07 LVVFDD463 pKa = 5.41 SALADD468 pKa = 3.65 KK469 pKa = 10.42 YY470 pKa = 11.59 LEE472 pKa = 3.89 IAKK475 pKa = 10.24 ILGVEE480 pKa = 3.95 INLTKK485 pKa = 10.57 SISSHH490 pKa = 5.94 DD491 pKa = 3.43 RR492 pKa = 11.84 PVFEE496 pKa = 4.56 FAKK499 pKa = 9.26 RR500 pKa = 11.84 TCFGNSDD507 pKa = 4.53 LSPISIKK514 pKa = 10.42 QLLSNDD520 pKa = 3.48 QLSEE524 pKa = 3.63 RR525 pKa = 11.84 TMNVVSFLKK534 pKa = 10.55 RR535 pKa = 11.84 GLLVSRR541 pKa = 11.84 SHH543 pKa = 8.04 IGILLSKK550 pKa = 10.18 FGSWQILKK558 pKa = 10.26 NKK560 pKa = 9.58 KK561 pKa = 9.56 LSKK564 pKa = 9.81 TPLLAILGILNSMNLISHH582 pKa = 6.94 RR583 pKa = 11.84 WLTEE587 pKa = 3.33 ALIDD591 pKa = 4.43 PKK593 pKa = 11.32 SDD595 pKa = 3.26 FDD597 pKa = 3.84 NLKK600 pKa = 10.76 DD601 pKa = 3.16 ITIPQEE607 pKa = 4.27 SIIKK611 pKa = 10.16 LIRR614 pKa = 11.84 EE615 pKa = 4.01 VGKK618 pKa = 10.56 VLNGEE623 pKa = 4.3 SLALQYY629 pKa = 10.24 PYY631 pKa = 11.42 SKK633 pKa = 10.06 MDD635 pKa = 3.77 DD636 pKa = 3.33 RR637 pKa = 11.84 GEE639 pKa = 4.19 IYY641 pKa = 10.54 DD642 pKa = 4.27 DD643 pKa = 3.91 YY644 pKa = 11.43 LVEE647 pKa = 4.25 FANVIANTAYY657 pKa = 9.39 TKK659 pKa = 11.02 AKK661 pKa = 10.17 ALEE664 pKa = 4.19 ANMMNLISKK673 pKa = 7.38 EE674 pKa = 3.88 AKK676 pKa = 9.21 NLYY679 pKa = 10.45 NSFEE683 pKa = 4.16 KK684 pKa = 11.04 SQDD687 pKa = 3.21 PTLASAIEE695 pKa = 4.42 GWFEE699 pKa = 4.15 DD700 pKa = 4.06 LLYY703 pKa = 10.91 SDD705 pKa = 5.01 GVFDD709 pKa = 4.72 IDD711 pKa = 4.14 EE712 pKa = 4.63 LVDD715 pKa = 4.28 SIEE718 pKa = 4.39 SNKK721 pKa = 10.04 AFYY724 pKa = 10.4 YY725 pKa = 10.3 RR726 pKa = 11.84 QLDD729 pKa = 3.68 MEE731 pKa = 4.53 KK732 pKa = 10.65 ALAIEE737 pKa = 4.15 DD738 pKa = 3.78 QVNKK742 pKa = 10.41 FIFNYY747 pKa = 9.95 EE748 pKa = 3.85 LTPQEE753 pKa = 4.11 FNSINVEE760 pKa = 3.89 SAPIMKK766 pKa = 9.71 ILSKK770 pKa = 10.53 IVGGHH775 pKa = 4.61 SSRR778 pKa = 11.84 YY779 pKa = 10.18 LKK781 pKa = 10.14 IQRR784 pKa = 11.84 PNN786 pKa = 2.89
Molecular weight: 89.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.561
IPC2_protein 8.478
IPC_protein 8.331
Toseland 9.165
ProMoST 8.916
Dawson 9.428
Bjellqvist 9.151
Wikipedia 9.575
Rodwell 9.75
Grimsley 9.487
Solomon 9.458
Lehninger 9.428
Nozaki 9.282
DTASelect 9.107
Thurlkill 9.282
EMBOSS 9.604
Sillero 9.385
Patrickios 4.94
IPC_peptide 9.458
IPC2_peptide 7.614
IPC2.peptide.svr19 7.692
Protein with the highest isoelectric point:
>tr|A0A0S4GAV3|A0A0S4GAV3_9VIRU RNA dependent RNA polymerase OS=Botrytis cinerea mitovirus 3 OX=1629666 GN=RdRp PE=4 SV=1
MM1 pKa = 7.76 KK2 pKa = 9.36 ITRR5 pKa = 11.84 TYY7 pKa = 11.31 KK8 pKa = 10.6 NLNSLLKK15 pKa = 10.68 SSIVKK20 pKa = 7.38 TKK22 pKa = 10.85 KK23 pKa = 9.28 MFSIRR28 pKa = 11.84 DD29 pKa = 3.78 SISNSLSIPLRR40 pKa = 11.84 QIQYY44 pKa = 10.93 LSFGKK49 pKa = 10.05 IRR51 pKa = 11.84 GLANRR56 pKa = 11.84 ALITRR61 pKa = 11.84 DD62 pKa = 2.91 FMTFVLKK69 pKa = 10.01 MKK71 pKa = 9.87 KK72 pKa = 9.35 NHH74 pKa = 6.37 GADD77 pKa = 4.26 FTIKK81 pKa = 9.34 WLKK84 pKa = 8.89 CCYY87 pKa = 9.82 VALQKK92 pKa = 11.13 SLGKK96 pKa = 10.45 DD97 pKa = 3.02 NLEE100 pKa = 4.03 SLRR103 pKa = 11.84 DD104 pKa = 3.83 LEE106 pKa = 4.96 PNLPLPRR113 pKa = 11.84 LINGIPAIISSNDD126 pKa = 2.94 RR127 pKa = 11.84 KK128 pKa = 10.47 LIRR131 pKa = 11.84 EE132 pKa = 3.89 MDD134 pKa = 3.56 SRR136 pKa = 11.84 IIIYY140 pKa = 9.45 WSSLFSLYY148 pKa = 10.18 RR149 pKa = 11.84 VLKK152 pKa = 10.38 CSYY155 pKa = 9.35 KK156 pKa = 10.85 LKK158 pKa = 10.29 ISSITDD164 pKa = 3.46 PFKK167 pKa = 11.33 GSINEE172 pKa = 4.11 FNDD175 pKa = 3.92 IIQGPVFSIFFDD187 pKa = 3.6 RR188 pKa = 11.84 LNGFPEE194 pKa = 4.29 LVKK197 pKa = 10.73 KK198 pKa = 11.08 SNLAPSKK205 pKa = 10.62 VRR207 pKa = 11.84 LLRR210 pKa = 11.84 SSSSSNNVSWHH221 pKa = 6.44 GIITDD226 pKa = 3.07 SWNISNSKK234 pKa = 9.86 EE235 pKa = 3.69 MSSNVSNYY243 pKa = 9.54 LASIKK248 pKa = 10.72 ALGWNTMWFNSKK260 pKa = 10.03 LNEE263 pKa = 4.17 MIEE266 pKa = 4.22 LGDD269 pKa = 3.86 RR270 pKa = 11.84 LNQVGSLKK278 pKa = 9.81 TKK280 pKa = 10.44 KK281 pKa = 10.31 SLSGQFGQFSLKK293 pKa = 10.46 EE294 pKa = 3.73 EE295 pKa = 4.19 AAGKK299 pKa = 10.03 LRR301 pKa = 11.84 IFAIVDD307 pKa = 4.02 SITQSLLSPLHH318 pKa = 7.02 DD319 pKa = 4.61 FMFDD323 pKa = 3.56 LLKK326 pKa = 10.64 KK327 pKa = 10.03 IPNDD331 pKa = 3.47 GTFDD335 pKa = 3.24 QDD337 pKa = 3.69 LSVKK341 pKa = 10.13 RR342 pKa = 11.84 SQVKK346 pKa = 10.2 SLSSGKK352 pKa = 10.26 AFSFDD357 pKa = 3.28 LSAATDD363 pKa = 3.94 RR364 pKa = 11.84 LPVDD368 pKa = 3.99 LTVKK372 pKa = 10.03 ILSKK376 pKa = 10.49 IFSDD380 pKa = 4.48 EE381 pKa = 4.98 FGTSWKK387 pKa = 10.27 QLMVNRR393 pKa = 11.84 DD394 pKa = 3.51 FFFSLTNQKK403 pKa = 10.99 DD404 pKa = 3.48 YY405 pKa = 11.34 GAPEE409 pKa = 3.99 SLRR412 pKa = 11.84 YY413 pKa = 9.59 SVGQPMGALSSWPALALTHH432 pKa = 6.47 HH433 pKa = 7.57 WILQYY438 pKa = 10.7 CSNILGRR445 pKa = 11.84 TGWEE449 pKa = 3.65 EE450 pKa = 3.73 NYY452 pKa = 10.47 EE453 pKa = 4.03 ILGDD457 pKa = 3.9 DD458 pKa = 4.07 LVVFDD463 pKa = 5.41 SALADD468 pKa = 3.65 KK469 pKa = 10.42 YY470 pKa = 11.59 LEE472 pKa = 3.89 IAKK475 pKa = 10.24 ILGVEE480 pKa = 3.95 INLTKK485 pKa = 10.57 SISSHH490 pKa = 5.94 DD491 pKa = 3.43 RR492 pKa = 11.84 PVFEE496 pKa = 4.56 FAKK499 pKa = 9.26 RR500 pKa = 11.84 TCFGNSDD507 pKa = 4.53 LSPISIKK514 pKa = 10.42 QLLSNDD520 pKa = 3.48 QLSEE524 pKa = 3.63 RR525 pKa = 11.84 TMNVVSFLKK534 pKa = 10.55 RR535 pKa = 11.84 GLLVSRR541 pKa = 11.84 SHH543 pKa = 8.04 IGILLSKK550 pKa = 10.18 FGSWQILKK558 pKa = 10.26 NKK560 pKa = 9.58 KK561 pKa = 9.56 LSKK564 pKa = 9.81 TPLLAILGILNSMNLISHH582 pKa = 6.94 RR583 pKa = 11.84 WLTEE587 pKa = 3.33 ALIDD591 pKa = 4.43 PKK593 pKa = 11.32 SDD595 pKa = 3.26 FDD597 pKa = 3.84 NLKK600 pKa = 10.76 DD601 pKa = 3.16 ITIPQEE607 pKa = 4.27 SIIKK611 pKa = 10.16 LIRR614 pKa = 11.84 EE615 pKa = 4.01 VGKK618 pKa = 10.56 VLNGEE623 pKa = 4.3 SLALQYY629 pKa = 10.24 PYY631 pKa = 11.42 SKK633 pKa = 10.06 MDD635 pKa = 3.77 DD636 pKa = 3.33 RR637 pKa = 11.84 GEE639 pKa = 4.19 IYY641 pKa = 10.54 DD642 pKa = 4.27 DD643 pKa = 3.91 YY644 pKa = 11.43 LVEE647 pKa = 4.25 FANVIANTAYY657 pKa = 9.39 TKK659 pKa = 11.02 AKK661 pKa = 10.17 ALEE664 pKa = 4.19 ANMMNLISKK673 pKa = 7.38 EE674 pKa = 3.88 AKK676 pKa = 9.21 NLYY679 pKa = 10.45 NSFEE683 pKa = 4.16 KK684 pKa = 11.04 SQDD687 pKa = 3.21 PTLASAIEE695 pKa = 4.42 GWFEE699 pKa = 4.15 DD700 pKa = 4.06 LLYY703 pKa = 10.91 SDD705 pKa = 5.01 GVFDD709 pKa = 4.72 IDD711 pKa = 4.14 EE712 pKa = 4.63 LVDD715 pKa = 4.28 SIEE718 pKa = 4.39 SNKK721 pKa = 10.04 AFYY724 pKa = 10.4 YY725 pKa = 10.3 RR726 pKa = 11.84 QLDD729 pKa = 3.68 MEE731 pKa = 4.53 KK732 pKa = 10.65 ALAIEE737 pKa = 4.15 DD738 pKa = 3.78 QVNKK742 pKa = 10.41 FIFNYY747 pKa = 9.95 EE748 pKa = 3.85 LTPQEE753 pKa = 4.11 FNSINVEE760 pKa = 3.89 SAPIMKK766 pKa = 9.71 ILSKK770 pKa = 10.53 IVGGHH775 pKa = 4.61 SSRR778 pKa = 11.84 YY779 pKa = 10.18 LKK781 pKa = 10.14 IQRR784 pKa = 11.84 PNN786 pKa = 2.89
Molecular weight: 89.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.561
IPC2_protein 8.478
IPC_protein 8.331
Toseland 9.165
ProMoST 8.916
Dawson 9.428
Bjellqvist 9.151
Wikipedia 9.575
Rodwell 9.75
Grimsley 9.487
Solomon 9.458
Lehninger 9.428
Nozaki 9.282
DTASelect 9.107
Thurlkill 9.282
EMBOSS 9.604
Sillero 9.385
Patrickios 4.94
IPC_peptide 9.458
IPC2_peptide 7.614
IPC2.peptide.svr19 7.692
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
786
786
786
786.0
89.12
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.835 ± 0.0
0.636 ± 0.0
5.98 ± 0.0
4.707 ± 0.0
5.216 ± 0.0
4.707 ± 0.0
1.145 ± 0.0
8.651 ± 0.0
8.397 ± 0.0
12.468 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.29 ± 0.0
6.234 ± 0.0
3.181 ± 0.0
3.053 ± 0.0
4.198 ± 0.0
11.959 ± 0.0
3.562 ± 0.0
4.198 ± 0.0
1.654 ± 0.0
2.926 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here