Pseudoglutamicibacter albus
Average proteome isoelectric point is 6.02
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1813 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2I1J8L4|A0A2I1J8L4_9MICC ABC transporter ATP-binding protein OS=Pseudoglutamicibacter albus OX=98671 GN=CJ193_03140 PE=4 SV=1
MM1 pKa = 7.79 SDD3 pKa = 2.88 VKK5 pKa = 11.25 KK6 pKa = 10.44 NLRR9 pKa = 11.84 YY10 pKa = 9.44 SAEE13 pKa = 4.25 HH14 pKa = 6.17 EE15 pKa = 4.34 WVDD18 pKa = 3.64 DD19 pKa = 4.15 SNPVKK24 pKa = 10.94 VGISQVAADD33 pKa = 3.85 ALGDD37 pKa = 3.73 VVYY40 pKa = 11.25 VDD42 pKa = 4.38 LPEE45 pKa = 5.44 AGSEE49 pKa = 4.14 VTAGEE54 pKa = 4.19 TCGEE58 pKa = 4.21 VEE60 pKa = 4.36 STKK63 pKa = 10.75 SVSDD67 pKa = 3.7 LYY69 pKa = 11.43 APVSGTIVEE78 pKa = 4.26 INQAVIDD85 pKa = 5.06 DD86 pKa = 4.31 PALLNSDD93 pKa = 4.14 PYY95 pKa = 11.33 GEE97 pKa = 4.43 GWLFTVEE104 pKa = 4.15 VTSEE108 pKa = 4.34 GEE110 pKa = 3.91 LLSAEE115 pKa = 5.08 DD116 pKa = 3.76 YY117 pKa = 11.46 ASANGGNLVV126 pKa = 3.42
Molecular weight: 13.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.773
IPC2_protein 3.706
IPC_protein 3.656
Toseland 3.465
ProMoST 3.821
Dawson 3.643
Bjellqvist 3.795
Wikipedia 3.567
Rodwell 3.49
Grimsley 3.376
Solomon 3.617
Lehninger 3.579
Nozaki 3.77
DTASelect 3.935
Thurlkill 3.516
EMBOSS 3.579
Sillero 3.783
Patrickios 1.825
IPC_peptide 3.617
IPC2_peptide 3.757
IPC2.peptide.svr19 3.722
Protein with the highest isoelectric point:
>tr|A0A2I1J9P1|A0A2I1J9P1_9MICC Thiamine-phosphate synthase OS=Pseudoglutamicibacter albus OX=98671 GN=thiE PE=3 SV=1
MM1 pKa = 7.51 TKK3 pKa = 9.09 RR4 pKa = 11.84 TFQPNNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 SKK15 pKa = 10.39 KK16 pKa = 9.03 HH17 pKa = 4.36 GFRR20 pKa = 11.84 LRR22 pKa = 11.84 MRR24 pKa = 11.84 TRR26 pKa = 11.84 AGRR29 pKa = 11.84 AILSTRR35 pKa = 11.84 RR36 pKa = 11.84 NKK38 pKa = 9.43 GRR40 pKa = 11.84 KK41 pKa = 8.9 SLSAA45 pKa = 3.86
Molecular weight: 5.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.705
Grimsley 13.086
Solomon 13.539
Lehninger 13.451
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.427
IPC_peptide 13.554
IPC2_peptide 12.53
IPC2.peptide.svr19 9.21
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1813
0
1813
615517
32
1666
339.5
36.71
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.103 ± 0.079
0.573 ± 0.016
5.941 ± 0.05
6.683 ± 0.06
3.105 ± 0.038
8.432 ± 0.045
2.172 ± 0.028
4.706 ± 0.039
3.422 ± 0.049
9.313 ± 0.059
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.224 ± 0.021
2.695 ± 0.03
5.125 ± 0.042
3.414 ± 0.032
6.426 ± 0.053
5.763 ± 0.037
5.999 ± 0.048
8.445 ± 0.062
1.389 ± 0.02
2.07 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here