Brochothrix thermosphacta (Microbacterium thermosphactum)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Listeriaceae; Brochothrix

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2406 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1D2LFT7|A0A1D2LFT7_BROTH Transcriptional regulator OS=Brochothrix thermosphacta OX=2756 GN=CNY62_10265 PE=4 SV=1
MM1 pKa = 7.42NWTNYY6 pKa = 9.27PDD8 pKa = 3.74SLSVDD13 pKa = 3.47EE14 pKa = 5.33LLEE17 pKa = 4.24KK18 pKa = 10.66LAADD22 pKa = 3.87QITDD26 pKa = 3.51YY27 pKa = 11.42KK28 pKa = 11.1EE29 pKa = 3.86YY30 pKa = 11.2LLFNAYY36 pKa = 9.01EE37 pKa = 4.15FQEE40 pKa = 4.35KK41 pKa = 10.32LCAIYY46 pKa = 10.35EE47 pKa = 4.15KK48 pKa = 10.84LKK50 pKa = 11.36AEE52 pKa = 4.16DD53 pKa = 3.75TVVLNLYY60 pKa = 9.87NPNLDD65 pKa = 3.72SGDD68 pKa = 4.05DD69 pKa = 3.82LLVAMTIDD77 pKa = 3.34GDD79 pKa = 4.62YY80 pKa = 10.65IIEE83 pKa = 4.18QNKK86 pKa = 6.16TTLVVPVSLEE96 pKa = 3.63AAAVEE101 pKa = 4.67VYY103 pKa = 9.9PYY105 pKa = 10.39PAVQFLKK112 pKa = 10.67AYY114 pKa = 6.98TNKK117 pKa = 10.31QITSNILADD126 pKa = 4.55IIDD129 pKa = 3.53

Molecular weight: 14.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1D2KFH0|A0A1D2KFH0_BROTH TatD family deoxyribonuclease OS=Brochothrix thermosphacta OX=2756 GN=CNY62_06120 PE=4 SV=1
MM1 pKa = 7.14KK2 pKa = 9.31QTYY5 pKa = 7.54QPKK8 pKa = 9.49KK9 pKa = 7.65RR10 pKa = 11.84KK11 pKa = 8.69HH12 pKa = 5.94SKK14 pKa = 8.54VHH16 pKa = 5.68GFRR19 pKa = 11.84ARR21 pKa = 11.84MSSKK25 pKa = 9.98NGRR28 pKa = 11.84RR29 pKa = 11.84VLAARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.81GRR39 pKa = 11.84KK40 pKa = 8.75VLSAA44 pKa = 4.05

Molecular weight: 5.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2406

0

2406

729719

12

2998

303.3

33.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.705 ± 0.063

0.622 ± 0.013

5.246 ± 0.046

6.824 ± 0.055

4.404 ± 0.046

6.445 ± 0.048

1.89 ± 0.022

7.929 ± 0.055

6.959 ± 0.055

9.745 ± 0.066

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.68 ± 0.027

4.699 ± 0.04

3.326 ± 0.029

3.706 ± 0.036

3.762 ± 0.041

6.002 ± 0.048

6.396 ± 0.044

7.218 ± 0.044

0.877 ± 0.017

3.568 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski