Pseudomonas phage MR4

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Gajwadongvirus; unclassified Gajwadongvirus

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 70 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M3T997|A0A6M3T997_9CAUD Uncharacterized protein OS=Pseudomonas phage MR4 OX=2711171 GN=PssvBMR4_gp46 PE=4 SV=1
MM1 pKa = 7.27NFSFTRR7 pKa = 11.84DD8 pKa = 2.99TGDD11 pKa = 3.02GVKK14 pKa = 9.33TVFTMSFAGQDD25 pKa = 2.99EE26 pKa = 5.47GYY28 pKa = 10.14ISASNIHH35 pKa = 6.16VYY37 pKa = 10.65VNGVEE42 pKa = 4.44VPFTINPADD51 pKa = 3.85PNKK54 pKa = 10.15VYY56 pKa = 11.1LSTAPSNGADD66 pKa = 3.32VLIRR70 pKa = 11.84RR71 pKa = 11.84IMPKK75 pKa = 9.89NVPYY79 pKa = 11.04SDD81 pKa = 4.23FKK83 pKa = 11.33NGNPFSQDD91 pKa = 2.88TLNYY95 pKa = 8.15TQLQQLYY102 pKa = 7.85VTQEE106 pKa = 4.06LLDD109 pKa = 3.98GFLPDD114 pKa = 4.32GFYY117 pKa = 10.87FKK119 pKa = 10.84QDD121 pKa = 3.38VNMGGYY127 pKa = 9.72NIKK130 pKa = 10.66NLGEE134 pKa = 4.29AVDD137 pKa = 4.48PDD139 pKa = 3.89DD140 pKa = 4.44AVKK143 pKa = 10.91KK144 pKa = 10.37EE145 pKa = 3.99VTDD148 pKa = 3.57NLGNRR153 pKa = 11.84VSSLEE158 pKa = 4.29DD159 pKa = 3.32NLTDD163 pKa = 3.75TSLTTIPWRR172 pKa = 11.84FVATGGEE179 pKa = 4.5VTISPPYY186 pKa = 9.45TFTNALLYY194 pKa = 10.57INGVTQTLGADD205 pKa = 3.41YY206 pKa = 10.57AYY208 pKa = 9.39TVSGNQLHH216 pKa = 6.5LSEE219 pKa = 4.4PLRR222 pKa = 11.84YY223 pKa = 9.12GDD225 pKa = 3.63EE226 pKa = 4.12VFVIVGSNLQPPTDD240 pKa = 3.97IISYY244 pKa = 9.11DD245 pKa = 3.43GAMIAYY251 pKa = 9.45ASMVTGVPIQQVYY264 pKa = 10.14IPVIGLSVNNALVFIEE280 pKa = 4.23NGVIHH285 pKa = 6.41TLSSPATGTITSISWPDD302 pKa = 3.13IVISS306 pKa = 3.78

Molecular weight: 33.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M3T949|A0A6M3T949_9CAUD Uncharacterized protein OS=Pseudomonas phage MR4 OX=2711171 GN=PssvBMR4_gp30 PE=4 SV=1
MM1 pKa = 7.63IHH3 pKa = 6.88INEE6 pKa = 3.96LSIYY10 pKa = 10.08RR11 pKa = 11.84LRR13 pKa = 11.84KK14 pKa = 8.43LAKK17 pKa = 8.04EE18 pKa = 4.11KK19 pKa = 10.17RR20 pKa = 11.84WSGLGIAYY28 pKa = 9.97RR29 pKa = 11.84YY30 pKa = 10.64VKK32 pKa = 10.5FKK34 pKa = 10.66KK35 pKa = 10.02LSKK38 pKa = 9.62LRR40 pKa = 11.84PVIRR44 pKa = 11.84EE45 pKa = 3.82IQVGEE50 pKa = 4.21TLVYY54 pKa = 10.27AYY56 pKa = 7.56QTLLVQPCGGTRR68 pKa = 11.84AVTWVRR74 pKa = 11.84SLFGNEE80 pKa = 3.47YY81 pKa = 9.21TAVEE85 pKa = 4.22DD86 pKa = 4.08FKK88 pKa = 11.57GIRR91 pKa = 11.84KK92 pKa = 8.5CHH94 pKa = 4.68ATYY97 pKa = 10.66RR98 pKa = 11.84SGDD101 pKa = 3.7KK102 pKa = 10.66ILVVTGWRR110 pKa = 3.45

Molecular weight: 12.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

70

0

70

14090

29

1287

201.3

22.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.822 ± 0.489

1.121 ± 0.179

6.018 ± 0.216

6.331 ± 0.327

3.577 ± 0.197

7.104 ± 0.239

1.93 ± 0.224

4.982 ± 0.215

6.593 ± 0.304

8.673 ± 0.239

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.236 ± 0.175

4.414 ± 0.279

3.605 ± 0.168

4.592 ± 0.305

4.855 ± 0.222

6.224 ± 0.3

5.848 ± 0.293

6.863 ± 0.311

1.455 ± 0.151

3.754 ± 0.192
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski