Lake Sarah-associated circular virus-12

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 8.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A140AQK4|A0A140AQK4_9VIRU Coat protein OS=Lake Sarah-associated circular virus-12 OX=1685738 PE=4 SV=1
MM1 pKa = 8.01AYY3 pKa = 10.22NFCFTCNNPTDD14 pKa = 4.92AIEE17 pKa = 4.41TYY19 pKa = 9.46IQSITDD25 pKa = 3.15VRR27 pKa = 11.84FIGYY31 pKa = 8.68AHH33 pKa = 6.81EE34 pKa = 4.45VGEE37 pKa = 4.85LGTPHH42 pKa = 6.27LQGVVVLNKK51 pKa = 9.9KK52 pKa = 10.01SRR54 pKa = 11.84LTAVRR59 pKa = 11.84KK60 pKa = 9.53ILPKK64 pKa = 10.4CHH66 pKa = 6.5ISQMRR71 pKa = 11.84GSFAEE76 pKa = 3.94AWDD79 pKa = 4.7GYY81 pKa = 10.62ILTPHH86 pKa = 7.14AKK88 pKa = 9.6HH89 pKa = 6.11NKK91 pKa = 8.37PEE93 pKa = 4.26PEE95 pKa = 3.8VVYY98 pKa = 11.02AFGTPPGVRR107 pKa = 11.84GSNTRR112 pKa = 11.84DD113 pKa = 3.3VYY115 pKa = 11.02EE116 pKa = 3.95EE117 pKa = 3.83AMAIAKK123 pKa = 8.47TGTTEE128 pKa = 4.56GISAQMMLRR137 pKa = 11.84FGKK140 pKa = 10.18ALHH143 pKa = 6.39EE144 pKa = 4.46VAQKK148 pKa = 10.58HH149 pKa = 5.29SPKK152 pKa = 9.81PPHH155 pKa = 7.02IDD157 pKa = 3.51GDD159 pKa = 3.99LDD161 pKa = 3.77NYY163 pKa = 9.53WIYY166 pKa = 11.27GPTGSGKK173 pKa = 10.69SKK175 pKa = 9.47TVYY178 pKa = 10.49DD179 pKa = 3.35KK180 pKa = 10.76WPRR183 pKa = 11.84DD184 pKa = 3.26TLYY187 pKa = 10.59IKK189 pKa = 10.4LHH191 pKa = 5.32NKK193 pKa = 6.68WWEE196 pKa = 4.0NYY198 pKa = 9.85DD199 pKa = 3.62GEE201 pKa = 4.42EE202 pKa = 3.69TVYY205 pKa = 10.6IEE207 pKa = 5.87DD208 pKa = 4.71LGNDD212 pKa = 3.72SKK214 pKa = 11.7LLTHH218 pKa = 6.87IKK220 pKa = 9.72HH221 pKa = 5.44WADD224 pKa = 3.54RR225 pKa = 11.84YY226 pKa = 10.57AFRR229 pKa = 11.84AEE231 pKa = 4.14VKK233 pKa = 10.27FGSILIRR240 pKa = 11.84PKK242 pKa = 10.6RR243 pKa = 11.84LVVTSNHH250 pKa = 6.21TIRR253 pKa = 11.84DD254 pKa = 4.0SFPTASVADD263 pKa = 3.86VEE265 pKa = 4.23ALEE268 pKa = 4.26RR269 pKa = 11.84RR270 pKa = 11.84FKK272 pKa = 10.39IQYY275 pKa = 9.44IGPQIPAFTSVTKK288 pKa = 10.79

Molecular weight: 32.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A140AQK4|A0A140AQK4_9VIRU Coat protein OS=Lake Sarah-associated circular virus-12 OX=1685738 PE=4 SV=1
MM1 pKa = 7.56PAVKK5 pKa = 8.92KK6 pKa = 8.07TFRR9 pKa = 11.84KK10 pKa = 9.68GKK12 pKa = 7.8SAKK15 pKa = 8.82RR16 pKa = 11.84TSNKK20 pKa = 7.77STKK23 pKa = 7.68TKK25 pKa = 10.17KK26 pKa = 9.99PKK28 pKa = 9.64ASKK31 pKa = 9.89VFKK34 pKa = 10.59KK35 pKa = 10.38KK36 pKa = 9.4VEE38 pKa = 4.01RR39 pKa = 11.84VIASQAEE46 pKa = 4.4VKK48 pKa = 10.55HH49 pKa = 5.84ISTWGAPAYY58 pKa = 9.96YY59 pKa = 10.33NSAIAGAGDD68 pKa = 3.94INPILPPMFQGTKK81 pKa = 9.2DD82 pKa = 3.32RR83 pKa = 11.84EE84 pKa = 4.37RR85 pKa = 11.84IGSKK89 pKa = 10.23VNFRR93 pKa = 11.84SFRR96 pKa = 11.84LNICISQAPYY106 pKa = 11.44ALLTDD111 pKa = 4.25LDD113 pKa = 4.06RR114 pKa = 11.84PVLVRR119 pKa = 11.84LLVLTYY125 pKa = 10.64KK126 pKa = 10.34RR127 pKa = 11.84GKK129 pKa = 8.5YY130 pKa = 9.23QPDD133 pKa = 3.49FTTSRR138 pKa = 11.84IVTDD142 pKa = 3.55VNEE145 pKa = 4.42SLLHH149 pKa = 5.73QDD151 pKa = 3.65ASAPAQPYY159 pKa = 10.03DD160 pKa = 3.95GSILHH165 pKa = 6.7HH166 pKa = 6.77LFPVNNDD173 pKa = 2.95LFTVIKK179 pKa = 10.24DD180 pKa = 3.13ISFTILPNEE189 pKa = 4.36LNDD192 pKa = 3.39NTTNSQTTALGKK204 pKa = 9.96NFRR207 pKa = 11.84TFSVNVPGPSHH218 pKa = 6.86LVYY221 pKa = 11.02GEE223 pKa = 5.7DD224 pKa = 3.46INQYY228 pKa = 9.56PNNFAPFFSVGYY240 pKa = 9.1CYY242 pKa = 10.09PDD244 pKa = 4.11GDD246 pKa = 4.57LADD249 pKa = 3.83VLNTGLRR256 pKa = 11.84VTWEE260 pKa = 3.93SRR262 pKa = 11.84LSYY265 pKa = 10.38TDD267 pKa = 3.32EE268 pKa = 4.03

Molecular weight: 29.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

556

268

288

278.0

31.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.014 ± 0.209

0.899 ± 0.107

5.576 ± 0.277

4.496 ± 1.062

4.496 ± 0.511

6.115 ± 0.626

3.058 ± 0.837

6.115 ± 0.626

7.554 ± 0.198

7.014 ± 0.839

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.259 ± 0.36

5.216 ± 1.054

6.295 ± 0.296

2.698 ± 0.202

5.216 ± 0.006

6.475 ± 0.956

7.734 ± 0.072

6.835 ± 0.179

1.439 ± 0.486

4.496 ± 0.275
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski