Bacillus phage vB_BhaS-171

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 66 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0U4K541|A0A0U4K541_9CAUD Uncharacterized protein OS=Bacillus phage vB_BhaS-171 OX=1775140 GN=BhaS171_00027 PE=4 SV=1
MM1 pKa = 7.67NITPDD6 pKa = 2.95ILRR9 pKa = 11.84EE10 pKa = 4.15FKK12 pKa = 11.05NRR14 pKa = 11.84MHH16 pKa = 6.85LGDD19 pKa = 4.39YY20 pKa = 10.92EE21 pKa = 4.75DD22 pKa = 5.81DD23 pKa = 3.53NLARR27 pKa = 11.84ILSASNQALLKK38 pKa = 10.75ACGPYY43 pKa = 10.27DD44 pKa = 3.62INTDD48 pKa = 3.42EE49 pKa = 4.36TFKK52 pKa = 11.07EE53 pKa = 4.39LVFEE57 pKa = 4.52RR58 pKa = 11.84SRR60 pKa = 11.84YY61 pKa = 8.86VYY63 pKa = 11.06NDD65 pKa = 2.85ALEE68 pKa = 4.1YY69 pKa = 10.89FNTNFLSQINSLGIEE84 pKa = 4.1KK85 pKa = 10.35ALEE88 pKa = 4.36EE89 pKa = 4.66IVLEE93 pKa = 4.54DD94 pKa = 5.28DD95 pKa = 5.09ADD97 pKa = 3.67ATLL100 pKa = 4.07

Molecular weight: 11.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0U4IBD0|A0A0U4IBD0_9CAUD MazG nucleotide pyrophosphohydrolase domain protein OS=Bacillus phage vB_BhaS-171 OX=1775140 GN=BhaS171_00062 PE=4 SV=1
MM1 pKa = 7.03TQFKK5 pKa = 10.31EE6 pKa = 4.38LIEE9 pKa = 4.26TVQMTTGLSKK19 pKa = 10.62RR20 pKa = 11.84DD21 pKa = 3.51IQRR24 pKa = 11.84HH25 pKa = 5.31LDD27 pKa = 3.55VSEE30 pKa = 4.43HH31 pKa = 7.81SMFIWQRR38 pKa = 11.84SGPPSSKK45 pKa = 10.0MPLIMSKK52 pKa = 10.53LKK54 pKa = 10.59EE55 pKa = 4.5LIHH58 pKa = 5.78TPKK61 pKa = 9.74TINVSAYY68 pKa = 8.42YY69 pKa = 9.74SFRR72 pKa = 11.84RR73 pKa = 11.84RR74 pKa = 11.84GMFDD78 pKa = 3.17YY79 pKa = 11.0QIAQKK84 pKa = 10.19WGICKK89 pKa = 10.33SSLRR93 pKa = 11.84KK94 pKa = 8.88WKK96 pKa = 9.55RR97 pKa = 11.84DD98 pKa = 3.17HH99 pKa = 7.2SIGRR103 pKa = 11.84RR104 pKa = 11.84GAA106 pKa = 3.26

Molecular weight: 12.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

66

0

66

11782

34

694

178.5

20.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.018 ± 0.456

0.696 ± 0.118

6.018 ± 0.24

8.157 ± 0.441

4.303 ± 0.313

6.62 ± 0.456

1.961 ± 0.254

6.968 ± 0.209

8.751 ± 0.438

8.335 ± 0.267

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.699 ± 0.193

5.602 ± 0.362

2.988 ± 0.192

3.785 ± 0.216

4.439 ± 0.367

5.882 ± 0.217

5.602 ± 0.31

6.221 ± 0.262

1.239 ± 0.141

3.718 ± 0.227
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski