Bat cyclovirus GF-4c

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; Cyclovirus; Bat associated cyclovirus 1

Average proteome isoelectric point is 8.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D9IZ81|D9IZ81_9CIRC Putative Cap OS=Bat cyclovirus GF-4c OX=795381 PE=4 SV=1
MM1 pKa = 7.37EE2 pKa = 5.26NKK4 pKa = 9.6TIRR7 pKa = 11.84RR8 pKa = 11.84FIFTWNNYY16 pKa = 8.38TDD18 pKa = 4.57DD19 pKa = 6.08DD20 pKa = 4.61YY21 pKa = 11.99DD22 pKa = 3.72KK23 pKa = 10.66TCAFIKK29 pKa = 10.29GNCKK33 pKa = 10.03YY34 pKa = 10.73GIVGKK39 pKa = 9.26EE40 pKa = 3.74HH41 pKa = 7.2APTTGVPHH49 pKa = 6.69LQGFCNLSKK58 pKa = 10.66PMRR61 pKa = 11.84FSAIKK66 pKa = 9.89KK67 pKa = 8.67CLSNSIHH74 pKa = 6.31IEE76 pKa = 3.76KK77 pKa = 10.55AIGSDD82 pKa = 3.42EE83 pKa = 4.04QNQKK87 pKa = 10.03YY88 pKa = 9.71CSKK91 pKa = 10.96AGDD94 pKa = 3.8FFEE97 pKa = 5.43EE98 pKa = 4.57GLPYY102 pKa = 9.93TQGKK106 pKa = 7.77RR107 pKa = 11.84TDD109 pKa = 3.5LEE111 pKa = 4.07AVVRR115 pKa = 11.84DD116 pKa = 3.94MEE118 pKa = 4.35QGTVRR123 pKa = 11.84LEE125 pKa = 4.59DD126 pKa = 3.15IAAKK130 pKa = 10.13HH131 pKa = 5.52PCAFIKK137 pKa = 8.34YY138 pKa = 9.62HH139 pKa = 6.87RR140 pKa = 11.84GIDD143 pKa = 3.5QLLKK147 pKa = 10.34LIHH150 pKa = 6.8PIKK153 pKa = 10.54PRR155 pKa = 11.84DD156 pKa = 3.56FQTYY160 pKa = 7.78VYY162 pKa = 9.12YY163 pKa = 10.77YY164 pKa = 8.96WGEE167 pKa = 4.08PGTGKK172 pKa = 10.02SRR174 pKa = 11.84RR175 pKa = 11.84AKK177 pKa = 10.62SEE179 pKa = 3.58ADD181 pKa = 3.28AVNSASIYY189 pKa = 8.73YY190 pKa = 9.87KK191 pKa = 10.51PRR193 pKa = 11.84GLWWDD198 pKa = 4.4GYY200 pKa = 9.51RR201 pKa = 11.84QQEE204 pKa = 4.04NVIIDD209 pKa = 4.38DD210 pKa = 4.37FYY212 pKa = 11.73GWIKK216 pKa = 10.75YY217 pKa = 10.28DD218 pKa = 4.1EE219 pKa = 4.44LLKK222 pKa = 10.71ICDD225 pKa = 3.53RR226 pKa = 11.84YY227 pKa = 9.88PYY229 pKa = 10.1KK230 pKa = 10.94VQIKK234 pKa = 10.43GGFEE238 pKa = 3.92EE239 pKa = 4.93FTSKK243 pKa = 10.88RR244 pKa = 11.84IWITSNVDD252 pKa = 2.54IYY254 pKa = 11.62SLYY257 pKa = 10.75KK258 pKa = 10.27FEE260 pKa = 5.17GYY262 pKa = 9.61NAEE265 pKa = 3.93AFKK268 pKa = 10.98RR269 pKa = 11.84RR270 pKa = 11.84ITCMVEE276 pKa = 3.78MNSNKK281 pKa = 10.17

Molecular weight: 32.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D9IZ81|D9IZ81_9CIRC Putative Cap OS=Bat cyclovirus GF-4c OX=795381 PE=4 SV=1
MM1 pKa = 7.28AAFRR5 pKa = 11.84RR6 pKa = 11.84RR7 pKa = 11.84RR8 pKa = 11.84RR9 pKa = 11.84VRR11 pKa = 11.84PRR13 pKa = 11.84KK14 pKa = 9.71NRR16 pKa = 11.84FGRR19 pKa = 11.84LRR21 pKa = 11.84RR22 pKa = 11.84RR23 pKa = 11.84RR24 pKa = 11.84FGRR27 pKa = 11.84RR28 pKa = 11.84IGRR31 pKa = 11.84RR32 pKa = 11.84KK33 pKa = 9.34VRR35 pKa = 11.84PNVLYY40 pKa = 10.73CKK42 pKa = 9.8LTRR45 pKa = 11.84TVTRR49 pKa = 11.84KK50 pKa = 9.13IDD52 pKa = 3.34LRR54 pKa = 11.84IATGSPNQIEE64 pKa = 4.25IQEE67 pKa = 3.97LRR69 pKa = 11.84INLNNFAEE77 pKa = 4.91HH78 pKa = 6.87INLASNFEE86 pKa = 4.37KK87 pKa = 10.53IKK89 pKa = 10.37ILKK92 pKa = 9.49QVVSVIPCQNVSNSTTSIQLHH113 pKa = 5.31YY114 pKa = 10.87AIVPYY119 pKa = 10.37KK120 pKa = 10.33KK121 pKa = 10.12EE122 pKa = 4.12SPALSTPFPAILSVDD137 pKa = 3.49KK138 pKa = 11.33AKK140 pKa = 10.36MISNTRR146 pKa = 11.84RR147 pKa = 11.84ASMALVPAAHH157 pKa = 6.66LHH159 pKa = 6.22SSGQTSDD166 pKa = 4.53DD167 pKa = 3.75MNKK170 pKa = 10.13LVYY173 pKa = 10.08KK174 pKa = 10.28PEE176 pKa = 3.86FSIPATGVSATIYY189 pKa = 9.83SGFVAFEE196 pKa = 4.21RR197 pKa = 11.84NSDD200 pKa = 3.53EE201 pKa = 4.48VFEE204 pKa = 5.15NKK206 pKa = 9.57QAWFVVKK213 pKa = 10.14QDD215 pKa = 3.28IYY217 pKa = 11.58VKK219 pKa = 10.75YY220 pKa = 9.75SVQRR224 pKa = 11.84SFII227 pKa = 3.98

Molecular weight: 26.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

508

227

281

254.0

29.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.299 ± 0.726

1.969 ± 0.663

4.724 ± 1.269

5.315 ± 0.823

5.118 ± 0.103

5.315 ± 1.361

1.969 ± 0.126

8.071 ± 0.086

8.465 ± 0.864

5.315 ± 0.52

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.772 ± 0.006

5.315 ± 0.52

4.134 ± 0.434

3.74 ± 0.137

8.268 ± 1.943

6.693 ± 1.291

4.921 ± 0.046

5.906 ± 1.503

1.378 ± 0.572

5.315 ± 1.361
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski